Delivery of surface-mediated non-viral gene nanoparticles from ultrathin layer-by-layer multilayers

作     者：WANG XueFei,REN KeFeng,LIN QuanKui & JI Jian Key Laboratory of Macromolecule Synthesis and Functionalization,Ministry of Education Department of Polymer Science and Engineering,Zhejiang University,Hangzhou 310027,China 

作者机构：Key Laboratory of Macromolecule Synthesis and Functionalization Ministry of Education Department of Polymer Science and Engineering Zhejiang University Hangzhou China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2010年第53卷第3期

页      面：508-513页

核心收录：

学科分类：07[理学] 070205[理学-凝聚态物理] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学] 

基　　金：supported by the National Natural Science Foundation of China (Grant No 50830106) the 863 Programm (Grant No 2006AA03Z329) Science and Technology Plan of Zhejiang Province of China (Grant No 2007C24008) 

主　　题：DNA nanoparticles layer-by-layer self-assembly biodegradability in situ transfection poly(lactic acid) scaffold 

摘      要：An efficient and safe gene delivery system remains a challenge in the development of gene ***-based gene nanoparticles are a typical non-viral gene delivery system,which are able to transfect cells in vitro and in *** paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly,in which the polycation-based gene nanoparticles were *** this surface-mediated delivery system,adherent cells on the multilayer could be transfected in *** nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the *** nanoparticles/PGA multilayers constructed on the scaffold surface could also realize in situ transfection on the adherent *** well-structured,easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles,which may have potential applications for gene therapy in tissue engineering and medical implants.