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Mutation analyses of integrated HBV genome in hepatitis B patients

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Journal of Genetics and Genomics 2008年第2期35卷 85-90页

作者： Peilin wang xiuhai wang Shuying Cong Hongming Ma Xuecheng Zhang Laboratory of Medical Genetics Medical College of Qingdao University Qingdao 266021 China Laboratory of Molecular Genetics College of Marine Life Science Ocean University of China Qingdao 266003 China

Little has been learnt in the last 30 years about detection of HBV genome as well as its mutation analysis between hepatitis B fathers （HBF） and their children. In this study, we used nest polymerase chain reaction （PCR）, fluorescence in situ hybridization （FISH）, and DNA sequencing analysis, to examine the integrated HBV genome in paraffin-embedded testis tissues, which were taken as samples from HBE and in peripheral blood mononuclear cells （PBMC） from 74 cases of HBFs and their children who were born after their fathers＇ HBV infection （caHBF）. We found that HBV DNA existed in testis tissues, mainly in the basilar parts of the seminiferous tubules, and also in PBMC of HBE It was also documented that there were point mutations of poly-loci, insertions and deletions of nucleotides in integrated HBV genomes, and the types of gene mutations in the HBFs were similar to those in caHBE This study addresses the major types of gene mutations in integrated HBV genome in human patients and also presents reliable evidence of possible genetic transmission of hepatitis B.

关键词： genome hepatitis B virus (HBV) gene mutation vertical transmission

Development and Application of an in Situ Penetrator for Rapid Strength Testing of Submarine Sediment

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Journal of Ocean University of China 2019年第2期18卷 328-338页

作者： ZHANG Minsheng wang Zhenhao wang xiuhai WEN Mingzheng HONG Bo School of Transportation Science and Engineering Beihang University Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering Ocean University of China

In marine engineering, the strength of a submarine sediment is an indispensable parameter for assessment of construction. In this study, a free-fall cone penetrator named IPen was developed to realize a rapid and efficient measurement of sediment strength. The equipment is characterized by modular design and self-contained data acquisition. It is equipped with an acceleration sensor, a water pressure sensor, and a piezocone penetration test(CPTu) probe. It is designed to be released from near seabed surface with a releaser and then fall freely to provide a higher penetration velocity. Its maximum working depth is approximately 2500 m and maximum penetration depth is approximately 3 m. To derive the correlation between penetration resistance and sediment strength, a calibrator was devised to determine the penetration-rate factor. In addition, the factor applicable to in situ test points was determined in laboratory experiments. In June 2016, the IPen was tested in situ in the South Yellow Sea, China, during a shared voyage funded by the National Science Foundation. Meanwhile, undisturbed column samples were collected for laboratory tests. Based on the in situ test results, it was demonstrated that the IPen could accurately record the working states of various sensors during the freely falling course. IPen test results reliably reflected the sediment strength at all the testing points when compared with laboratory calibration tests, in situ vane tests and penetration tests, laboratory penetration tests, and unconsolidated and undrained triaxial compression tests.

关键词： submarine sediment free-fall cone penetration test sediment strength the Yellow Sea penetration-rate factor

An Innovative Approach to Predicting Scour Depth Around Foundations Under Combined Waves and Currents in Large-Scale Tests Based on Small-Scale Tests

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Journal of Ocean University of China 2023年第3期22卷 637-648页

作者： HU Ruigeng LIU Hongjun LU Yao wang xiuhai SHI Wei College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Key Laboratory of Marine Environment and Ecology Ministry of EducationQingdao 266100China Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering Qingdao 266100China School of Civil Engineering Qingdao University of TechnologyQingdao 266033China

This study presents an innovative theoretical approach to predicting the scour depth around a foundation in large-scale model tests based on small-scale model tests under combined waves and *** the present approach,the hydrodynamic parameters were designed based on the Froude similitude *** avoid the cohesive behavior,we scaled the sediment size based on the settling velocity similarity,i.e.,the suspended load ***,a series of different scale model tests was conducted to obtain the scour depth around the pile in combined waves and *** fitting formula of scour depth from the small-scale model tests was used to predict the results of large-scale *** accuracy of the present approach was validated by comparing the prediction values with experimental data of large-scale ***,the correctness and accuracy of the present approach for foundations with complex shapes,e.g.,the tripod foundation,was further *** results indicated that the fitting line from small-scale model tests slightly overestimated the experimental data of large-scale model tests,and the errors can be *** general,the present approach was applied to predict the maximum or equilibrium scour depth of the large-scale model tests around single piles and tripods.

关键词： scour scour depth prediction Froude similarity scale effects combined waves and currents

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Research on Corrosion Effect of Sulfate Ions on Concrete Under Wetting-Drying Cycle

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Journal of Ocean University of China 2022年第1期21卷 124-130页

作者： wang xiuhai TIAN Zhuangcai ZHANG Yanan SU Xiuting LIU Hongjun LIU Tao College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering Ocean University of ChinaQingdao 266100China State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China

Saline soil is widely distributed in the marine sediments along the coast of the world and the arid-semi-arid areas of the Middle East and Iraq,and calcium sulfate erosion has become one of the important factors affecting the durability of concrete in this *** order to clarify the mechanism of sulfate ion damage to concrete,this paper mainly takes saline soil with high sulfate content in coastal area as well as arid-semi-arid area as the research object,and uses indoor geotechnical test,field test and numerical simulation to study the influence of different dry-wet cycle times on the unconfined compressive strength of concrete test blocks,and puts forward the relationship between the erosion arrival depth and time of sulfate ion in concrete,so as to predict the long-term erosion depth by using the erosion depth of sulfate ion in concrete in short *** results show that the shorter the erosion time when the erosion reaches a certain depth,and the larger the erosion reaches when the erosion time is the same,the faster the erosion reaches the depth with the increase of erosion *** with rectangular section concrete,circular section concrete penetrates *** results of this study can provide a reference for the durability design of concrete in saline soil sites containing sulfate.

关键词： wetting-drying cycle sulfate ion concrete corrosion

Impact of seepage flow on sediment resuspension by internal solitary waves:parameterization and mechanism

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Journal of Oceanology and Limnology 2023年第2期41卷 444-457页

作者： Zhuangcai TIAN Chao LIU Ziyin REN Xiujun GUO Mingwei ZHANG xiuhai wang Lei SONG Yonggang JIA State Key Laboratory for Geomechanics and Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China Key Laboratory of Coastal Science and Integrated Management Ministry of Natural ResourcesQingdao 266061China Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering Ocean University of ChinaQingdao 266100China

Sediment incipient motion is the first step in sediment *** studies ignored the effect of seepage flow on the mobility of sediment particles and simplified the seabed surface as a rigid boundary.A flume experiment was designed to innovatively divide the seabed into two parts to control the dynamic response of the seabed and control the seepage *** the experiment,the seabed sediments and the amplitude of internal solitary waves(ISWs)were changed to compare and analyze the impact of seepage flow on the sediment resuspension by shoaling ***,parametric research and verification were carried *** indicate that seepage flow can greatly influence fine sand,promote sediment resuspension,and increase the amount of suspension by two times on ***,seepage flow had a little effect on the suspension of clayey silt and sandy ***,seepage force was added to the traditional gravity,drag force,and uplift force,and the parameterization of threshold starting shear stress of coarse-grained sediments was *** results of this parameterization were verified,and seepage force was critical to *** threshold starting shear stress was reduced by 54.6%after increasing the seepage *** physical mechanism of this process corresponded to the vertical reciprocating transient seepage in and out the seabed interface caused by the wave-induced transient excess pore water *** quantitative study on seepage flow for shear stress of coarse-grained sediments induced by ISWs is critical to geohazard assessment.

关键词： internal waves seepage flow sediment resuspension parameterization mechanism

Selective special concentration and allelopathic effects of plant hormone Indole-3-acetic on growth promotion of marine photosynthetic bacteria

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Selective special concentration and allelopathic effects of ...

Allelopathic Effects of Three Plant Growth Hormones on the Growth of Photosynthetic Bacteria

中国第四届植物化感作用学术研讨会

作者： xiuhai wang LANSHENG LI YING LIU Key Lab of Marine Environmental Science and Ecology(Ministry of Education)Ocean University of China

We studied the effects of Indole-3-acetic(plant growth hormone)on the growth of photosynthetic bacteria(PSB).Indole-3-acetic promoted the growth of PSB at an optimal concentration of 1.0 mg/*** the presence of Indole-3-acetic,a deferred logarithmic growth phase *** the optimal concentration 1.0 mg/L in 0-5days,the slopes of the treated samples were 0.1121,0.1042,0.1208 and 0.0926 AU/day respectively,while the slope of the control sample was 0.1023 AU/*** OD660nm results representing growth promotion of PSB were statistically analyzed with SPSS:the treated samples were specially compared with control sample at the concentration 1.0 mg/L,and obviously the treated sample were higher than the control sample(P<0.05).In the deferred logarithmic growth phase(5-13 days),especially at the optimal concentration 0.1 mg/L(571.4 nmol/1), the slopes of the treated samples were 0.5928,0.3629,0.4851 and 0.4937 AU/day respectively,while the slope of the control sample was 0.4947AU/*** OD660nm results of promoting the growth of PSB were also statistically analyzed with SPSS:the treated sample were specially compare with control sample at the optimal concentration 0.1 mg/L,and obviously the treated sample were still higher than the control sample(P<0.01).Therefore,there was a significant growth promotion effect,a vivid phenomenon of allelopathic *** most significant growth promotion concentration is a selective special concentration,and the effect is named the selective special concentration allelopathic *** is first report on the allelopathic effects of Indole-3-acetic on growth promotion of PSB.

关键词： Plant hormone Indole-3-acetic selective special concentration selective special concentration allelopathic effect Photosynthetic Bacterium promotion culture promotion efficiency nanomolar.

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Allelopathic Effects of Three Plant Growth Hormones on the G...

中国第二届植物化感作用学术研讨会暨中国植物保护学会植物化感作用专业委员会成立大会

作者： Liu Ying Li Lansheng Li Fengmin wang xiuhai College of Environmental Science and Engineering Ocean University of China

Photosynthetic bacteria (PSB) is one kind of hydrosphere microorganisms. It grows with photo-heterotrophically light and anaerobic conditions and metabolizes various organic compounds aselectron donors and carbon sources. PSB is significant in both natural ecosystem and practical application. It has been used as an active microbial feed additive and applied in the treatment of organic waster water. Due to its important applied value, it has been receiving increasingly attention. Nevertheless the chief problem for PSB in practical application is how to obtain PSB culture solution with high concentration in shorter time and lower cost. Therefore the study about mass culture of PSB becomes important for application of PSB widely. In this paper, the growth promotion of triacontanol(TA), gibberellin(GA3) and α-naphthylacetic acid (α-NAA) on PSB was studied. The results showed that the three plant growth hormones could accelerate growth of PSB. Of the three, gibberellin exhibited the best promoting effect with optimum concentration of 0.5 mg/L. Optimum concentrations of triacontanol and α-naphthylacetic acid were both 1.0 mg/L. This indicated that it was feasible and practical to promote growth of PSB by adding some plant growth hormones.

关键词： Photosynthetic Bacteria (PSB) Triacontanol (TA) Gibberellin(GA3) α-NaphthylaceticAcid (α-NAA) Allelopathic Effect

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e型电子束蒸发源的原理及维修

半导体技术 2012年第5期37卷 386-389页

作者：王秀海中国电子科技集团公司第十三研究所石家庄050051

电子束蒸发由于其显著的优点应用日趋广泛,成为工业镀膜工艺中最主要的技术之一。基于真空镀膜技术,首先对电子束蒸发的定义进行了阐述,介绍了电子束蒸发源的特点及优势,详细说明了e型电子束蒸发源的结构及工作原理。重点分析了电子枪... 详细信息

电子束蒸发由于其显著的优点应用日趋广泛,成为工业镀膜工艺中最主要的技术之一。基于真空镀膜技术,首先对电子束蒸发的定义进行了阐述,介绍了电子束蒸发源的特点及优势,详细说明了e型电子束蒸发源的结构及工作原理。重点分析了电子枪灯丝与聚束极的相对位置对束斑及束流大小的影响。结合维修经验对e型电子束蒸发源的日常维护进行了总结,对更换电子枪灯丝步骤及注意事项进行了细致说明,着重提出了腔室及蒸发源的洁净度对工艺质量的影响。最后对于蒸发过程中常见故障现象及解决方法进行说明。

关键词：电子束电子枪束斑故障现象维修

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快速退火炉中影响工艺质量的因素分析

半导体技术 2012年第7期37卷 528-530,561页

作者：王秀海中国电子科技集团公司第十三研究所石家庄050051

基于快速热处理技术,对快速退火炉的特点进行了阐述,介绍了快速退火炉的腔室结构,从综合分析设备的基础上阐述了影响工艺质量的主要因素。加热灯使用寿命会影响腔室的温度均匀性,更换加热灯和调整各灯组的功率因子可以提高温度均匀性。... 详细信息

基于快速热处理技术,对快速退火炉的特点进行了阐述,介绍了快速退火炉的腔室结构,从综合分析设备的基础上阐述了影响工艺质量的主要因素。加热灯使用寿命会影响腔室的温度均匀性,更换加热灯和调整各灯组的功率因子可以提高温度均匀性。保护气体流量对晶片的边缘效应会导致晶片受热不均匀进而影响到工艺的均匀性,调整保护气体流量也是保证工艺质量的关键。金属腔体的洁净度会影响到热辐射的均匀性和升温速率,必须定期清理。石英腔体的洁净程度会影响到温度测量的精度,应定期清理腔体并进行温度校正。

关键词：快速退火加热灯温度测量红外辐射均匀性