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CEA and AFP expression in human hepatoma cells transfected with antisense IGF-I gene

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World Journal of Gastroenterology 1998年第1期4卷 35-37页

作者： ZHANG Li LI Shu-Nong wang xiao-ning Department of Immunology First Military Medical University Guangzhou 510515 Guangdong Province China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences Guangzhou 510089 Guangdong Province China

IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using Lipofectin reagent. Northern blot analysis confirmed the antisense IGFI RNA of the transfected cells. CEA and AFP levels were measured using radioimmunoassay.RESULTS Human hepatoma cell lines (HepG2) were transfected with antisense IGFI gene. Northern blot analysis confirmed that antisense IGFI RNA was expressed in the transfected cells. The effect of antisense IGFI gene on CEA and AFP expression was demonstrated by the fact that the CEA and AFP levels in the supernatant of transfected cell culture were significantly lower as compared with the parent cells, 〔CEA 70μg/L±076μg/L and 329μg/L±180μg/L (P<005) and AFP 5363μg/L±602μg/L and 90μg/L±526μg/L (P<001), respectively〕.CONCLUSION The malignant potentiality of the transfected cells was partially suppressed. Antisense IGFI gene can modulate the expression of CEA and AFP in human hepatoma cell lines (HepG2).

关键词： insulinlike growth factor-I carcinoembryonic antigen αfetoprotein carcinoma,hepatocellular tumor cells,cultured radioimmunoassay

输尿管软镜碎石术联合可弯曲负压输尿管导引鞘与经皮肾镜取石术治疗2~3cm肾结石的疗效比较

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赣南医学院学报 2024年第6期44卷 580-584,621页

作者：冷松李方志王晓宁赣南医科大学第一临床医学院江西赣州341000 赣南医科大学第一附属医院泌尿外科江西赣州341000

目的:比较输尿管软镜碎石术(Flexible ureteroscopy lithotripsy,FURL)联合可弯曲负压输尿管导引鞘与经皮肾镜取石术(Percutaneous nephrolithotomy,PCNL)治疗2~3 cm肾结石的效果。方法:分析2022年1月至2023年2月收治的151例2~3 cm肾结石患者的临床资料。根据手术方式分为FURL组(86例)与PCNL组(65例)。比较2组患者术前资料、总手术时间、清石时间、术后住院时间、血红蛋白下降值、结石清除率及术后并发症。结果:2组患者术前一般资料比较差异无统计学意义(P>0.05)。FURL组与PCNL组术后1天结石清除率分别为81.40%、86.15%,术后1月结石清除率分别为87.21%、89.23%,差异均无统计学意义(P>0.05)。与PCNL组相比,FURL组清石时间较长[(66.78±25.87)min vs(47.23±16.70)min],术后住院时间更短(3 d vs 5 d),差异均有统计学意义(P0.05)。与PCNL组相比,FURL组血红蛋白下降更少[(5.16±7.48)g·L^(-1) vs(10.32±7.48)g·L^(-1)],术后并发症发生率更低,差异均有统计学意义(P<0.05)。结论:FURL联合可弯曲输尿管导引鞘是一种安全有效的手术方式,可作为治疗2~3 cm肾结石的选择方案。

关键词：输尿管鞘输尿管软镜经皮肾镜取石术肾结石

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墨苋涂膜剂的制备及质量控制

中国药物经济学 2024年第8期19卷 104-110页

作者：孔鹃亓恒芹王小宁济宁市皮肤病防治院药剂科制剂室山东济宁272100 山东大学药学院济南250100

目的对墨苋涂膜剂制备优选并进行质量控制研究。方法以成膜时间、成膜性状、黏度为考察指标,成膜性能综合评分为评价指标,考察成膜材料、增塑剂、促透剂,利用L9(3^(4))正交试验,筛选最优制备工艺;采用薄层色谱法对墨旱莲、马齿苋、冰片... 详细信息

目的对墨苋涂膜剂制备优选并进行质量控制研究。方法以成膜时间、成膜性状、黏度为考察指标,成膜性能综合评分为评价指标,考察成膜材料、增塑剂、促透剂,利用L9(3^(4))正交试验,筛选最优制备工艺;采用薄层色谱法对墨旱莲、马齿苋、冰片进行鉴别;利用高效液相色谱法对蟛蜞菊内酯含量进行测定。结果最优制备工艺处方为:6%PVA0588,2%甘油,0.5%吐温-80;3味药材薄层色谱斑点清晰,专属性好,阴性无干扰;蟛蜞菊内酯在4.04~101.09μg/ml范围内线性关系良好(R^(2)=0.9996),平均回收率为100.6%,RSD为1.97%(n=9)。结论墨苋涂膜剂制备工艺简单可行,建立的质控方法科学准确、易操作,可用于墨苋涂膜剂的质量控制。

关键词：墨苋涂膜剂制备工艺正交试验质量控制蟛蜞菊内酯

亚甲基四氢叶酸脱氢酶2是异质性细胞叠套结构形成的负调控分子

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中国生物化学与分子生物学报 2024年第6期40卷 819-826页

作者：冯鹏飞刘辰瑜黄一诺孙卓然张揚易黄红艳王晨曦王小宁解放军医学院研究生院北京100853 解放军总医院第二医学中心老年医学研究所衰老与相关疾病研究北京市重点实验室国家老年疾病临床医学研究中心北京100853 军事医学研究院生物工程研究所北京100071 首都医科大学附属北京世纪坛医院肿瘤治疗性疫苗北京市重点实验室北京100038

异质性细胞叠套结构(heterotypic cell-in-cell structure,heCICs)介导独特的非自主性细胞死亡,广泛参与肿瘤发生、发展和临床预后等多种重要的病理学过程。亚甲基四氢叶酸脱氢酶2(methylenetetrahydrofolata dehydrogenase 2,MTHFD2)作为一碳代谢的关键酶之一,在多种肿瘤细胞中高表达。在本研究中,为了探究MTHFD2对heCICs形成能力的影响,首先应用活细胞染料对肝癌细胞和免疫细胞分别进行标记,利用荧光显微镜对细胞进行拍摄分析建立heCICs模型。进一步通过RNAi技术瞬时敲低细胞中的MTHFD 2,结果显示,MTHFD 2敲低后,PLC/PRF/5和Hep3B分别与免疫细胞形成heCICs的能力显著升高(均P<0.01)。通过同源重组方法构建MTHFD2重组表达质粒,进一步构建MTHFD2过表达细胞系;将过表达细胞系与免疫细胞共培养检测MTHFD2过表达对heCICs形成能力的影响,结果显示,过表达MTHFD2后heCICs形成率显著降低(均P<0.001)。综上,本研究表明,MTHFD2是heCICs形成的负调控因子,为靶向MTHFD2促进heCICs形成增强免疫细胞胞内杀伤提供了研究基础。

关键词：亚甲基四氢叶酸脱氢酶2 异质性细胞叠套结构肝癌免疫治疗

Ethanol promotes saturated fatty acid-induced hepatoxicity through endoplasmic reticulum(ER) stress response

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Chinese Journal of Natural Medicines 2015年第4期13卷 250-256页

作者： YI Hong-Wei MA Yu-Xiang wang xiao-ning wang Cui-Fen LU Jian CAO Wei WU Xu-Dong Department of Pharmacology Medical School Southeast University State Key Laboratory of Pharmaceutical Biotechnology School of Life Sciences Nanjing University Center for Diagnostic Nanosystems Marshall University

Serum palmitic acid(PA), a type of saturated fatty acid, causes lipid accumulation and induces toxicity in ***(Et OH) is metabolized by the liver and induces hepatic injury and inflammation. Herein, we analyzed the effects of Et OH on PA-induced lipotoxicity in the liver. Our results indicated that Et OH aggravated PA-induced apoptosis and lipid accumulation in primary rat hepatocytes in dose-dependent manner. Et OH intensified PA-caused endoplasmic reticulum(ER) stress response in vitro and in vivo, and the expressions of CHOP, ATF4, and XBP-1 in nucleus were significantly increased. Et OH also increased PA-caused cleaved caspase-3 in cytoplasm. In wild type and CHOP–/– mice treated with Et OH and high fat diet(HFD), Et OH worsened the HFD-induced liver injury and dyslipidemia, while CHOP knockout blocked toxic effects of Et OH and PA. Our study suggested that targeting UPR-signaling pathways is a promising, novel approach to reducing Et OH and saturated fatty acid-induced metabolic complications.

关键词： Ethanol(Et OH) Palmitic acid(PA) ER stress CHOP knockout Liver toxicity

Microbial transformation of diosgenin by Syncephalastrum racemosum(Cohn) Schroeter

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Chinese Chemical Letters 2010年第1期21卷 76-80页

作者： Zhao, Ying Sun, Ling Mei wang, xiao ning Shen, Tao Ji, Mei Li, Xia Lou, Hong Xiang Shandong Univ Sch Pharmaceut Sci Dept Nat Prod Chem Jinan 250012 Peoples R China

Microbial transformation of diosgenin(1) by Syncephalastrum racemosum yielded five new polar metabolites,which wereidentified as(25R)-spirost-5-en-3β,7α,9α-triol-12-one(2),(25R)-spirost-5-en-3β,9α,12α-triol-7-one(3),(25R)-spirost-5-en-3β,9α-diol-7,12-dione(4),(25R)-spirost-4-en-9α,12β,14α-triol-3-one(5),and(25S)-spirost-4-en-9α,14α,25β-triol-3-one(6).Compounds 1-6 exhibited moderate cytotoxicity against K562 cells and among them compounds 2,3,and 6 were more potentthan the parent compound 1.

关键词： Biotransformation Diosgenin Syncephalastrum racemosum Cytotoxicity

Evaluation of soil flame disinfestation(SFD) for controlling weeds,nematodes and fungi

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Journal of Integrative Agriculture 2020年第1期19卷 164-172页

作者： wang xiao-ning CAO Ao-cheng YAN Dong-dong wang Qian HUANG Bin ZHU Jia-hong wang Qiu-xia LI Yuan OUYANG Can-bin GUO Mei-xia wang Qian Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Foreign Economic Cooperation Office Ministry of Ecological EnvironmentBeijing 100035P.R.China

Soil flame disinfestation(SFD) is a form of physical disinfestation that can be used both in greenhouses and on field crops. Its use for soil disinfestation in different crop growing conditions makes it increasingly attractive for controlling soilborne pathogens and weeds. But little is known about the effect on weeds and soilbrone diseases. This study reports on greenhouses and field crops in China that determined the efficacy of SFD to control weeds, nematodes and fungi. It also determined the impact of SFD on the soil physical and chemical properties(water content, bulk density, NO3^–-N content, NH4^+-N content, conductivity and organic matter) in three field trials. A second generation SFD machine was used in these trials. SFD treatment significantly reduced weeds(>87.8%) and root-knot nematodes(Meloidogyne incognita)(>98.1%). Plant height and crop yield was significantly increased with SFD treatment. NO3^–-N and NH4^+-N increased after the SFD treatment, and there was also an increase in soil conductivity. Water content, bulk density and organic matter decreased significantly in the soil after the SFD treatment compared to the control. Soil flame disinfestation is a potential technique for controlling weeds and diseases in greenhouses or in fields. SFD is a non-chemical, safe, environmentally-friendly soil disinfection method.

关键词： soil flame disinfestation weeds pests control soil properties field application sustainable

Effect of Y_(2)O_(3) on microstructure and properties of CoCrFeNiTiNb high entropy alloy coating on Ti-6Al-4V surface by laser cladding

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Journal of Rare Earths 2024年第3期42卷 586-599,I0006页

作者： Zhen Li Wei Zhao Kedong Yu ning Guo Guangchun xiao Zhiming wang Hui Zhang College of Mechanical Engineering Qilu University of Technology(Shandong Academy of Sciences)Jinan250353China Shandong Institute of Mechanical Design and Research Jinan250031China

The effects of Y_(2)O_(3) on the microstructure, microhardness, wear resistance, high-temperature oxidation resistance, hot corrosion resistance, and electrochemical corrosion behaviour of CoCrFeNiTiNb high entropy alloy coatings formed on Ti-6Al-4V alloy surfaces were studied. The results show that the addition of Y_(2)O_(3) changes the proportion of the phase but does not change its type. The average grain size is only 1/4.7 of that of the high entropy alloy(HEA) coating, and the fine-grained strengthening leads to increases in the microhardness and wear resistance of 21.8% and 26.9%, respectively. The addition of Y_(2)O_(3) enhances the denseness and bonding properties of the oxide and corrosion product layers, reducing the oxidation and hot corrosion rates by 60.3% and 40.3%, respectively. The addition of Y_(2)O_(3) doubles the corrosion resistance which is attributed to the refinement of the grains, the increased proportion of HCP and TiN, and the weakening of galvanic coupling corrosion.

关键词： Laser cladding High entropy alloy coatings Rare earths Wear properties High temperature properties Electrochemical corrosion

The synergistic advantage of combining chloropicrin or dazomet with fosthiazate nematicide to control root-knot nematode in cucumber production

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Journal of Integrative Agriculture 2019年第9期18卷 2093-2106页

作者： HUANG Bin wang Qian GUO Mei-xia FANG Wen-sheng wang xiao-ning wang Qiu-xia YAN Dong-dong OUYANG Can-bin LI Yuan CAO Ao-cheng Institute of Plant Protection Chinese Academy of Agricultural SciencesBeijing 100193P.R.China Beijing Innovation Consortium of Agriculture Research System Beijing 100193P.R.China

The highly-damaging root-knot nematode(Meloidogyne spp.,RKN)cannot be reliably controlled using only a nematicide such as fosthiazate because of increasing pest *** laboratory and greenhouse trials,we showed that chloropicrin(CP)or dazomet(DZ)synergized the efficacy of fosthiazate against *** combination significantly extended the degradation half-life of fosthiazate by an average of about 1.25 *** or DZ with fosthiazate reduced the time for fosthiazate to penetrate the RKN cuticle compared to fosthiazate *** or DZ combined with low or medium rate of fosthiazate increased the total cucumber yield,compared to the use of each product alone.A low-dose fosthiazate with DZ improved total yield more than a low dose fosthiazate with *** the half-life of fosthiazate and reducing the time for fosthiazate or fumigant to penetrate the RKN cuticle were the two features that gave the fumigant-fosthiazate combination its synergistic advantage over these products used *** synergy provides the opportunity for farmers to use a low dose of fosthiazate which lowers the risk of RKN *** could combine DZ at 30 g m^-2 with fosthiazate at a low rate of 0.375 g m^-2 to control RKN and adequately control two major soil-borne diseases in cucumber greenhouses.

关键词： dazomet chloropicrin fosthiazate cucumber root-knot nematode