CEA and AFP expression in human hepatoma cells transfected with antisense IGF-I gene

作     者：ZHANG Li LI Shu-Nong WANG Xiao-Ning 

作者机构：Department of Immunology First Military Medical University Guangzhou 510515 Guangdong Province China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences Guangzhou 510089 Guangdong Province China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences China 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：1998年第4卷第1期

页      面：35-37页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：insulinlike growth factor-I carcinoembryonic antigen αfetoprotein carcinoma,hepatocellular tumor cells,cultured radioimmunoassay 

摘      要：IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using Lipofectin reagent. Northern blot analysis confirmed the antisense IGFI RNA of the transfected cells. CEA and AFP levels were measured using radioimmunoassay.RESULTS Human hepatoma cell lines (HepG2) were transfected with antisense IGFI gene. Northern blot analysis confirmed that antisense IGFI RNA was expressed in the transfected cells. The effect of antisense IGFI gene on CEA and AFP expression was demonstrated by the fact that the CEA and AFP levels in the supernatant of transfected cell culture were significantly lower as compared with the parent cells, 〔CEA 70μg/L±076μg/L and 329μg/L±180μg/L (P005) and AFP 5363μg/L±602μg/L and 90μg/L±526μg/L (P001), respectively〕.CONCLUSION The malignant potentiality of the transfected cells was partially suppressed. Antisense IGFI gene can modulate the expression of CEA and AFP in human hepatoma cell lines (HepG2).