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Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large White×Minzhu Intercross Porcine Population

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Journal of Integrative Agriculture 2014年第12期13卷 2721-2730页

作者： liU Xin wang li-gang liANG Jing YAN Hua ZHAO Ke-bin li Na ZHANG Long-chao wang li-xian Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation Ministry of Agriculture/Institute of Animal Science C hinese Academy of Agricultural Sciences Jilin Academy of Agricultural Sciences

Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip and were phenotyped for 10 traits, speciifcally, backfat thickness （6-7 libs）, carcass length, carcass weight, foot weight, head weight, heart weight, leaf fat weight, liver weight, lung weight and slaughter body weight. The genome-wide association study （GWAS） was assessed by Genome Wide Rapid Association using the mixed model and regression-genomic control approach. A total of 31 single nucleotide polymorphisms （SNPs）（with the most signiifcant SNP being MARC0033464, P value=6.80×10-13） were located in a 9.76-Mb （31.24-41.00 Mb） region on SSC7 and were found to be signiifcantly associated with one or more carcass traits and organ weights. High percentage of phenotypic variance explanation was observed for each trait ranging from 31.21 to 67.42%. linkage analysis revealed one haplotype block of 495 kb, in which the most signiifcant SNP being MARC0033464 was contained, on SSC7 at complete linkage disequilibrium. Annotation of the pig reference genome suggested 6 genes （GRM4, HMGA1, NUDT3, RPS10, SPDEF and PACSIN1） in this candidate linkage disequilibrium （LD） interval. Functional analysis indicated that the HMGA1 gene presents the prime biological candidate for carcass traits and organ weights in pig, with potential application in breeding programs.

关键词： genome-wide association study (GWAS) carcass trait HMGA1 gene organ weight pig

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Identification and Molecular Mapping of the Rs Dm R Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish(Raphanus sativus L.)

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Journal of Integrative Agriculture 2014年第11期13卷 2362-2369页

作者： XU liang JIANG Qiu-wei WU Jian wang Yan GONG Yi-qin wang xian-li limera Cecilia liU li-wang National Key Laboratory of Crop Genetics and Germplasm Enhancement/Engineering Research Center of Horticultural Crop Germplasm Enhancement and Utilization Ministry of Education/College of Horticulture Nanjing Agricultural University Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences

Downy mildew （DM）, caused by the fungus Peronospora parasitica, is a destructive disease of radish （Raphanus sativus L.） worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically iftted a 3：1 ratio in two F2 populations （F2（SR） and F2（RS））, and 1：1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs （1 036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR） were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers （Em9/ga24370, NAUISSR826700 and Me7/em10400） linked to the RsDmR locus within a 10.0 cM distance were identiifed using bulked segregant analysis （BSA）. The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs.

关键词： Raphanus sativus L. molecular mapping downy mildew seedling-stage resistance marker assisted selection

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考虑海底静摩擦的深水悬链锚泊线静力特性分析

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工程与建设 2024年第1期38卷 1-6页

作者：王硕汪利先万展宏王福硕牛小万武汉理工大学船海与能源动力工程学院湖北武汉430063 水沙科学与水灾害防治湖南省重点实验室湖南长沙410114

为探究海底静摩擦对于锚泊线张力分布的影响机制,基于悬链线模型,建立考虑海底静摩擦的锚泊线系统静力分析模型,开发锚泊线静力计算程序StaticMoor并完成锚泊线静力分析。研究表明:同一顶端激励下,海底静摩擦系数对锚泊线构型与锚泊线... 详细信息

为探究海底静摩擦对于锚泊线张力分布的影响机制,基于悬链线模型,建立考虑海底静摩擦的锚泊线系统静力分析模型,开发锚泊线静力计算程序StaticMoor并完成锚泊线静力分析。研究表明:同一顶端激励下,海底静摩擦系数对锚泊线构型与锚泊线悬垂段张力分布没有影响,而对锚泊线躺底段张力分布与锚固点张力存在明显的影响。

关键词：海底静摩擦顶端激励顶端张力悬链线

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Genome-wide association study for rib eye muscle area in a Large White×Minzhu F_2 pig resource population

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Journal of Integrative Agriculture 2015年第12期14卷 2590-2597页

作者： GUO Yun-yan ZHANG Long-chao wang li-xian liU Wen-zhong College of Animal Science and Veterinary Medicine Shanxi Agricultural University Institute of Animal Science Chinese Academy of Agricultural Sciences

Rib eye muscle area（REMA） is an economically important trait and one of the main selection criteria for breeding in the swine industry. In the genome-wide association study（GWAS）, the Illumina Porcine SNP60 Bead Chip containing 62 163 single nucleotide polymorphisms（SNPs） was used to genotype 557 pigs from a porcine Large White×Minzhu intercross population. The REMA（at the 5th–6th, 10th–11th and the last ribs） was measured after slaughtered at the age of（240±7） d for each animal. Association tests between REMA trait and SNPs were performed via the Genome-Wide Rapid Association using the Mixed Model and Regression-Genomic Control（GRAMMAR-GC） approach. From the Ensembl porcine database, SNP annotation was implemented using Sus scrofa Build 10.2. Thirty-three SNPs on SSC12 and 3 SNPs on SSC2 showed significant association with REMA at the last rib at the chromosome-wide significance level. None of the SNPs of REMA at the 5th–6th rib and only a few numbers of the SNPs of REMA at the 10th–11th ribs were found in this study. The Haploview V3.31 program and the *** R package were used to detect and visualize haplotype blocks and to analyze the association of the detected haplotype blocks with REMA at the last rib. A linkage analysis revealed that 4 haplotype blocks contained 4, 4, 2, and 4 SNPs, respectively. Annotations from pig reference genome suggested 2 genes（NOS2, NLK） in block 1（266 kb）, one gene（TMIGD1） in block 2（348 kb）, and one gene（MAP2K4） in block 3（453 kb）. A functional analysis indicated that MYH3 and MYH13 genes are the potential genes controlling REMA at the last rib. We screened several candidate intervals and genes based on the SNPs location and the gene function, and inferred that NOS2 and NLK genes maybe the main genes of REMA at the last ribs.

关键词： genome-wide association study rib eye muscle area pig SNP

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An adaptive 3D reconstruction method for asymmetric dual-angle multispectral stereo imaging system on UAV platform

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Science China(Information Sciences) 2024年第8期67卷 285-299页

作者： Chen wang xian li Yanfeng GU Zixu wang School of Electronics and Information Engineering Harbin Institute of Technology Beijing Institute of Control and Electronic Technology

A multispectral imaging system often cannot capture 3D spatial information owing to hardware limitations, which diminishes the effectiveness across various domains. To address this problem, we have developed a multispectral stereo imaging system along with an adaptive 3D reconstruction algorithm. Unlike existing unmanned aerial vehicle stereo imaging systems, our multispectral stereo imaging system uses two multispectral cameras with asymmetric spectral bands positioned at different angles. This design enables the acquisition of a higher number of bands and lateral spatial information while maintaining a lightweight structure. This system introduces challenges such as large geometric distortions and intensity differences between multiple bands. To accurately recover 3D spatial information, we propose an adaptive 3D reconstruction method. This method employs a position and orientation system-assisted projection transformation and a normalized threshold adjustment strategy. Finally, mutual information is used to reconstruct the multispectral images densely, effectively addressing nonlinear differences and generating a comprehensive multispectral point cloud. Our stereo system was used for two real data collections in different regions, and the efficacy of the proposed 3D reconstruction method was validated by comparing it with existing methods and commercial software.

关键词： multispectral images 3D reconstruction stereo imaging system unmanned aerial vehicle feature extraction

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超声细微特征鉴别卵巢多房囊性、囊实性肿瘤来源的价值

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河北医科大学学报 2024年第10期45卷 1212-1218页

作者：王礼贤魏晓轩王楠魏佳梁雅坤王翠菊河北医科大学第四医院妇产超声科河北石家庄050011

目的探讨超声细微特征在鉴别卵巢多房囊性、囊实性肿瘤来源的价值。方法选取河北医科大学第四医院超声诊断为卵巢多房囊性、囊实性肿瘤的患者246例作为研究对象,观察并记录肿瘤的超声细微特征。结合其临床特征和组织学分类,分析超声细... 详细信息

目的探讨超声细微特征在鉴别卵巢多房囊性、囊实性肿瘤来源的价值。方法选取河北医科大学第四医院超声诊断为卵巢多房囊性、囊实性肿瘤的患者246例作为研究对象,观察并记录肿瘤的超声细微特征。结合其临床特征和组织学分类,分析超声细微特征与卵巢原发性肿瘤、转移瘤的关系。结果246例卵巢肿瘤的组织学类型:原发性肿瘤217例(88.21%)和转移瘤29例(11.79%);在原发性肿瘤中,良性肿瘤62例(25.20%),以浆液性和黏液性囊腺瘤为主;交界性肿瘤27例(10.98%);恶性肿瘤128例(52.03%),以浆液性和黏液性囊腺癌为主;在转移瘤中,以原发部位结-直肠为主。临床特征:与原发性肿瘤相比,转移瘤的发病年龄较高,差异有统计学意义[(60.80±8.04)岁vs.(51.29±12.32)岁,P=0.035];原发性肿瘤组均无其他部位肿瘤的确诊病史,而转移瘤组中有9例在发现卵巢肿瘤时已有其他部位肿瘤确诊史(P0.05)。超声细微特征:与原发性肿瘤相比,卵巢转移瘤具有瘤体较大[(15.7±4.80)cm vs.(9.54±3.96)cm,P<0.001]、瘤内囊腔个数≥10者较多(55.17%vs.18.89%,P<0.001)、壁结节少见(6.90%vs.21.66%,P=0.007)但直径较大[(1.44±0.36)cm vs.(0.97±0.39)cm,P=0.031]以及彩色多普勒血流阻力指数低(0.46±0.07 vs.0.61±0.13,P<0.001)的特点。结论当超声诊断卵巢多房囊性、囊实性肿物时,特别是同时伴发其他部位恶性肿瘤,利用超声细微特征可提高鉴别卵巢肿物来源的能力。

关键词：卵巢肿瘤超声检查转移瘤

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天鲲号绞吸挖泥船挖岩作业配套船舶技术研究

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中国港湾建设 2024年第1期44卷 93-97页

作者：毛建辉费龙王显力丁勇张莹中国船舶与海洋工程设计研究院上海200011

随着全球经济增长和疏浚业的扩大,大型绞吸挖泥船已成为这一领域的关键装备。文章专注于提升超大型绞吸挖泥船“天鲲号”的效能。通过分析该船在挖岩工程中的挑战,引入了“挖-运分离”的高效物流策略。为实现这一目标,建议开发一艘专门... 详细信息

随着全球经济增长和疏浚业的扩大,大型绞吸挖泥船已成为这一领域的关键装备。文章专注于提升超大型绞吸挖泥船“天鲲号”的效能。通过分析该船在挖岩工程中的挑战,引入了“挖-运分离”的高效物流策略。为实现这一目标,建议开发一艘专门与天鲲号配套使用的开体式耙吸挖泥船。文章详细讨论了开体式耙吸挖泥船在主尺度和舱容、快速靠泊、泥舱结构和强度、疏浚系统和船舶布局等方面与天鲲号的匹配问题。提出了一个新颖的开体式耙吸挖泥船方案,能够完全满足配合天鲲号挖岩装驳和运输的需求,实现“挖-运分离”物流策略;能够开展后期扫浅挖泥作业,减少天鲲号的占用期,从而显著提升整体经济效益。

关键词：天鲲号绞吸挖泥船开体式耙吸挖泥船 “挖-运分离”策略装驳扫浅

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Multi-sensor multispectral reconstruction framework based on projection and reconstruction

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Science China(Information Sciences) 2024年第3期67卷 246-262页

作者： Tianshuai li Tianzhu liU xian li Yanfeng GU Yukun wang Yushi CHEN School of Electronics and Information Engineering Harbin Institute of Technology Heilongjiang Province Key Laboratory of Space-Air-Ground Integrated Intelligent Remote Sensing

The scarcity and low spatial resolution of hyperspectral images(HSIs) have become a major problem limiting the application of the images. In recent years, spectral reconstruction(SR) has been applied to convert multispectral images(MSIs) with abundant quantities and high spatial resolution into HSIs. With the launch of several new multispectral(MS) satellites with a short repeat period, the simultaneous acquisition of images from multiple MS sensors in the same area is gradually becoming feasible. Unfortunately, existing SR methods only consider the reconstruction of the MSIs of a single sensor without considering using MSIs from different MS sensors to obtain a better construction effect through their complementary bands. However,multi-sensor SR is characterized by two problems: inconsistency in the amplitude information of real multisensor imaging and difficulty in the extraction of the complex correlations of bands from different *** solve these problems, this paper proposes a multi-sensor SR framework based on a two-step approach in which the problems of amplitude inconsistency and band information extraction are solved using an ideal projection network and an ideal multi-sensor SR network, respectively. The effectiveness of the proposed method is verified by experiments on three datasets.

关键词： spectral reconstruction spectral superresolution hyperspectral image multispectral image neural networks multi-sensor

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Synthesis of highly concentrated,carrier free ^(188)Re-mercaptoacetyltriglycine

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Nuclear Science and Techniques 2002年第4期13卷 237-243页

作者： ZHANG Xiu-li, wang Yong-xian, li Jun-ling, ZHANG Chun-Fu, wang li-Hua,YIN Duan-Zhi(Shanghai Institute of Nuclear Research, the Chinese Academy of Sciences, Shanghai 201800) Shanghai Institute of Nuclear Research the Chinese Academy of Sciences 201800

Chelation of 188Re to compounds such as MAG3 will further reduce the radiation dose to the patient in case of balloon rupture through the rapid excretion from the body. In order to prepare highly concentrated, carrier free 188Re-MAG3, Sbenzoyl mercaptoacetyltriglycine (S-Bz-MAG3) was synthesized, labeled with carrier free 188Re. The overall yield of S-Bz-MAG3 is higher than those published in theliterature. Dependence of the labeling yield of 1ssRe-MAG3 upon concentrations of reducing agent, pH, reaction time, etc. was examined and optimum conditions were confirmed. The concentration procedure was succeeded with Sep-Pak C18 cartridge to obtain highly concentrated 1s8Re-MAG3. In the case of optimun conditions, the labeling yield of 188Re-MAG3 was more than 98%. Radiochemical purity of 188ReMAG3 was more than 92% after 24 hour storage at room temperature.

关键词：放射同位素 ^188Re 放射免疫疗法

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Upgrading waste NO_(x) into amino acids via electrocatalysis on Co nanoparticles encapsulated in hollow carbon nanofibers

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Science China Chemistry 2024年第6期67卷 1946-1952页

作者： Jiahui xian Kaixing Cai Peisen liao Shihan wang Guangqin li Key Laboratory of Bioinorganic and Synthetic Chemistry of Ministry of Education LIFMIGCMESchool of ChemistrySun Yat-Sen UniversityGuangzhou510275China

Electrocatalytic conversion of waste nitrogen oxide(NO_(x))into value-added nitrogenous chemicals,especially amino acid,is an emerging strategy to alleviate NO_(x)***,this process suffers from the coexistence of multiple competitive reactions leading to complex product distribution and low efficiency,and thus the rational design and modulation of efficient catalysts remains a formidable ***,well-designed Co nanoparticles encapsulated in hollow carbon nanofiber(CoHCNF)were constructed to promote the amino acid electrosynthesis via dealing waste NO_(x)coupling with biomass-derived keto *** to the rapid reactant diffusion,electron transport and efficient contact with abundant accessible Co catalytic sites stemmed from the unique hollow nanotubular structure,Co-HCNF manifested impressive glycine synthesis performance in one-pot adopting NO as the nitrogen source with Faradaic efficiency(FE)of 41.7%and a corresponding yield rate of 222.3μmol h^(-1)as well as 120 h robust ***,it also demonstrates an excellent performance with the FE of 42.8%for NO_(3)~-solution as the nitrogen ***,this strategy displays a broad universality for the synthesis of other amino *** work not only highlights the importance of hollow-structuring electrocatalyst towards upgrading wasted NO_(x)species into amino acids,but also promotes the further development of electrosynthetic systems for high-valued N-containing organic chemicals.

关键词： electrocatalysis NO_(x)reduction amino acid porous materials hollow structure

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