Genome-Wide Association Study for Certain Carcass Traits and Organ Weights in a Large White×Minzhu Intercross Porcine Population

作     者：LIU Xin WANG Li-gang LIANG Jing YAN Hua ZHAO Ke-bin LI Na ZHANG Long-chao WANG Li-xian 

作者机构：Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation Ministry of Agriculture/Institute of Animal Science C hinese Academy of Agricultural Sciences Jilin Academy of Agricultural Sciences 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2014年第13卷第12期

页      面：2721-2730页

核心收录：

学科分类：0710[理学-生物学] 0832[工学-食品科学与工程（可授工学、农学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0901[农学-作物学] 0703[理学-化学] 0902[农学-园艺学] 0713[理学-生态学] 

基　　金：supported by the Agricultural Science and Technology Innovation Program, China (ASTIPIAS02) the National Key Technology R&D Program of China (2011BAD28B01) the National Natural Science Foundation of China (31201781) the Earmarked Fund for Modern Agroindustry Technology Research System, National Technology Program of China (2011ZX08006-003) the Chinese Academy of Agricultural Sciences Foundation (2011cj-5, 2012ZL069 and 2014ywf-yb-8) 

主　　题：genome-wide association study (GWAS) carcass trait HMGA1 gene organ weight pig 

摘      要：Porcine carcass traits and organ weights have important economic roles in the swine industry. A total of 576 animals from a Large White×Minzhu intercross population were genotyped using the Illumina PorcineSNP60K Beadchip and were phenotyped for 10 traits, speciifcally, backfat thickness （6-7 libs）, carcass length, carcass weight, foot weight, head weight, heart weight, leaf fat weight, liver weight, lung weight and slaughter body weight. The genome-wide association study （GWAS） was assessed by Genome Wide Rapid Association using the mixed model and regression-genomic control approach. A total of 31 single nucleotide polymorphisms （SNPs） （with the most signiifcant SNP being MARC0033464, P value=6.80×10-13） were located in a 9.76-Mb （31.24-41.00 Mb） region on SSC7 and were found to be signiifcantly associated with one or more carcass traits and organ weights. High percentage of phenotypic variance explanation was observed for each trait ranging from 31.21 to 67.42%. Linkage analysis revealed one haplotype block of 495 kb, in which the most signiifcant SNP being MARC0033464 was contained, on SSC7 at complete linkage disequilibrium. Annotation of the pig reference genome suggested 6 genes （GRM4, HMGA1, NUDT3, RPS10, SPDEF and PACSIN1） in this candidate linkage disequilibrium （LD） interval. Functional analysis indicated that the HMGA1 gene presents the prime biological candidate for carcass traits and organ weights in pig, with potential application in breeding programs.