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COVID-19: a new challenge for human beings

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Cellular & Molecular Immunology 2020年第5期17卷 555-557页

作者： Penghui Yang xiliang wang The Fifth Medical Center of Chinese PLA General Hospital BeijingChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina

Since December 2019,just a month before the Chinese Spring Festival,multiple cases of pneumonia of unknown etiology appeared in Wuhan,Hubei Province,***,a novel coronavirus was identified in a bronchoalveolar lavage fluid sample from the Wuhan Seafood Market using metagenomic next-generation sequencing technology.1 On February 11,2020,the virus was named severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)by the International Committee on Taxonomy of Viruses(ICTV).SARS-CoV-2 is the seventh member of the coronavirus family that can infect humans after the emergence of severe acute respiratory syndrome coronavirus(SARS-CoV)and Middle East respiratory syndrome coronavirus(MERS-CoV).

关键词： technology. Wuhan Middle

Direct Comparative Analyses of 10X Genomics Chromium and Smart-seq2

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Genomics, Proteomics & Bioinformatics 2021年第2期19卷 253-266页

作者： xiliang wang Yao He Qiming Zhang Xianwen Ren Zemin Zhang BIOPIC Beijing Advanced Innovation Center for Genomicsand School of Life SciencesPeking UniversityBeijing 100871China Peking-Tsinghua Center for Life Sciences Academy for Advanced Interdisciplinary StudiesPeking UniversityBeijing 100871China

Single-cell RNA sequencing(scRNA-seq)is generally used for profiling transcriptome of individual *** droplet-based 10X Genomics Chromium(10X)approach and the plate-based Smart-seq2 full-length method are two frequently used scRNA-seq platforms,yet there are only a few thorough and systematic comparisons of their advantages and ***,by directly comparing the scRNA-seq data generated by these two platforms from the same samples of CD45cells,we systematically evaluated their features using a wide spectrum of ***-seq2 detected more genes in a cell,especially low abundance transcripts as well as alternatively spliced transcripts,but captured higher proportion of mitochondrial *** composite of Smart-seq2 data also resembled bulk RNA-seq data *** 10X-based data,we observed higher noise for mRNAs with low expression *** 10%30%of all detected transcripts by both platforms were from non-coding genes,with long non-coding RNAs(lncRNAs)accounting for a higher proportion in 10X.10X-based data displayed more severe dropout problem,especially for genes with lower expression ***,10X-data can detect rare cell types given its ability to cover a large number of *** addition,each platform detected distinct groups of differentially expressed genes between cell clusters,indicating the different characteristics of these *** study promotes better understanding of these two platforms and offers the basis for an informed choice of these widely used technologies.

关键词： Single-cell RNA sequencing 10X Smart-seq2 Bulk RNA-seq Comparison

Protection against lethal subcutaneous challenge of virulent Y. pestis strain 141 using an F1-V subunit vaccine

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Science China(Life Sciences) 2007年第5期50卷 600-604页

作者： wang Dong JIA Nuan LI Peng XING Li wang xiliang Institute of Microbiology and Epidemiology State Key Laboratory of Pathogen and BiosecurityAcademy of Military Medical Sci-encesBeijing 100071China School of Pharmacy Second Military Medical UniversityShanghai 200433China Hospital Attached to Aeromedicine Institute of PLA Beijing 100089China

In this study,we designed and engineered a two-component recombinant fusionprotein antigen as a vaccine candidate against the possible biological threat of Yersinia *** F1-V protein was formulated with Alhydrogel.A four-time injection with a dosage of10,20 and 50 ug/mouse in about two months was adopted for *** antibodies and subclassof T helper cells were measured and *** the final vaccination,the mice were challenged by141 strain with 25- 600 *** conclusion,the recombinant vaccine was capable of inducingprotective immunity against subcutaneous *** level of serum IgG was supposed to be a mainfactor that affected the final protection of challenge.20 ug recombinant protein could induce anendpoint titre of serum IgG as high as 51200,which was enough to afford 100% protection against 400LD50 virulent 141 *** antibody isotype analysis showed that the vaccine inducedpredominantly an lgG1 rather than lgG2a *** cytometric analysis revealed that Alhydrogelsignificantly helped induce a stronger humoral immunity instead of CTL cellular *** suggested that the plague F1-V subunit vaccine is promising for the next plague vaccine.

关键词： Yersinia pestis F1-V fusion protein challenge subunit vaccine

Identification of immunoreactive proteins of Brucella melitensis by immunoproteomics

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Science China(Life Sciences) 2011年第9期54卷 880-887页

作者： ZHAO ZhongPeng YAN Fang JI WenHui LUO DeYan LIU Xin XING Li DUAN YueQiang YANG PengHui SHI XiuMin LI Zhong wang xiliang State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China College of Animal Science and Veterinary Medicine Shanxi Agricultural University Taigu 030801 China

Infection with Brucella causes brucellosis, a chronic disease in humans, which induces abortion and sterility in livestock. Among the different Brucella species, Brucella melitensis is considered the most virulent and is the predominant species associated with outbreaks in China. To date, no safe human vaccine is available against Brucella infection. The currently used live vaccines against Brucella in livestock induce antibodies that interfere with the diagnosis of field infection in vaccinated ani- mals, which is harmful to eradication programs. However, there is as yet no complete profile of immunogenic proteins of B. melitensis. Towards the development of a safer, equally efficacious, and field infection-distinguishable vaccine, we used immunoproteomics to identify novel candidate immunogenic proteins from B. melitensis M5. Eighty-eight immunoreactive protein spots from B. melitensis M5 were identified by Western blotting and were assigned to sixty-one proteins by mass spectrometry, including many new immunoreactive proteins such as elongation factor G, FOFI ATP synthase subunit beta, and OMPI. These provide many candidate immunoreactive proteins for vaccine development.

关键词： Brucella immunoproteomics immunoreactive protein

Basic fibroblast growth factor protects against influenza A virus-induced acute lung injury by recruiting neutrophils

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Journal of Molecular Cell Biology 2018年第6期10卷 573-585页

作者： Keyu wang Chengcai Lai Tieling Li Cheng wang Wei wang Bing Ni Changqing Bai Shaogeng Zhang Lina Han Hongjing Gu Zhongpeng Zhao Yueqiang Duan Xiaolan Yang Li Xing Lingna Zhao Shanshan Zhou Min Xia Chengyu Jiang xiliang wang Penghui Yang State Key Laboratory of Pathogens and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Chinese PLA General Hospital Beijing 100853China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical SciencesBeijing 100005China Institute of Immunology Third Military Medical UniversityChongqing 400038China Beijing 307 Hospital of PLA Affiliated with the Chinese Academy of Medical Sciences Beijing 100070China Beijing 302 Hospital of PLA Beijing 100039China

Influenza virus (IAV)infection is a major cause of severe respiratory illness that affects almost every country in the *** infections result in respiratory illness and even acute lung injury and death,but the underlying mechanisms responsible for IAV pathogenesis have not yet been fully *** this study,the basic fibroblast growth factor 2 (FGF2)level was markedly increased in H1N1 virus-infected humans and ***2,which is predominately derived from epithelial cells,recruits and activates neutrophils via the FGFR2-PI3K-AKT-NFKB signaling ***2 depletion or knockout exacerbated influenzaassociated disease by impairing neutrophil recruitment and *** importantly,administration of the recombinant FGF2 protein significantly aUeviated the severity of IAV-induced lung injury and promoted the survival of IAV-infected *** on the results from experiments in which neutrophils were depleted and adoptively transferred,FGF2 protected mice against IAV , infection by recruiting ***,FGF2 plays a critical role in preventing IAV-induced lung injury,and FGF2 is a promising potential therapeutic target during IAV infection.

关键词： influenza H1N1 virus recombinant FGF2 protein neutrophil recruitment FGFR2-PI3K-AKT-NFκB signaling therapeutic target

The 2009 pandemic A/Wenshan/01/2009 H1N1 induces apoptotic cell death in human airway epithelial cells

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Journal of Molecular Cell Biology 2011年第4期3卷 221-229页

作者： Ning Yang Xiaoxu Hong Penghui Yang Xiangwu Ju Yuguo wang Jun Tang Chenggang Li Quanshui Fan Fuqiang Zhang Zhongwei Chen Li Xing Zhongpeng Zhao Xiao Gao Guoyang Liao Qihan Li xiliang wang Dangsheng Li Chengyu Jiang State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesPeking Union Medical CollegeTsinghua UniversityChinese Academy of Medical SciencesBeijing 100005China Department of Viral Immunology Institute of Medical BiologyChinese Academy of Medicine SciencePeking Union Medical CollegeKunming 650118China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China Centre for Disease Prevention and Control Cheng Du Military RegionKunming 650032China Shanghai Institutes for Biological Sciences Chinese Academy of SciencesShanghai 200031China

In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including *** 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly ***,we studied the infection and pathogenesis of a new 2009 pandemic strain,A/Wenshan/01/2009 H1N1,in China in human airway epithelial cell lines compared with contemporary seasonal H1N1 influenza *** results showed that viral infection by the A/Wenshan H1N1 induced significant apoptotic cell death in both the human nasopharyngeal carcinoma cell line CNE-2Z and the human lung adenocarcinoma cell line *** A/Wenshan H1N1 virus enters both of these cell types more efficiently than the seasonal influenza *** entry in both cell lines was shown to be mediated by clathrin-and dynamin-dependent ***,we discovered that the 2009 pandemic H1N1 strain,A/Wenshan/01/2009,can induce apoptotic cell death in epithelial cells of the human respiratory tract,suggesting a molecular pathogenesis for the 2009 pandemic H1N1.

关键词： apoptosis respiratory S-OIV H1N1 influenza virus

The Three Gorges Power Project Will PromotePower Network Interconnections throughout China

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Electricity 1998年第3期 6-8页

作者： wang xiliang(China Electric Power Information Center)

Immunological dynamics in response to two anthrax vaccines in mice

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Science China(Life Sciences) 2008年第10期51卷 872-878页

作者： Lü Jin, HE Rui, DONG Mei, ZHANG LiangYan & wang xiliang State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China 1. State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China

In order to understand the variation of humoral and cellular immune responses to A16R live spore and AVA vaccine and to identify efficient immunological parameters for the early evaluation of post immu- nization in mice, we dynamically monitored the antibody production and cellular responses after the vaccination of Balb/C mice with the anthrax vaccines. The results show that both anti-AVA and anti-Spore antibodies were detectable in the A16R live spore vaccinated group while high titers of anti-AVA antibodies but not anti-Spore antibodies existed in the AVA-immunized group. IgG1 and IgG2 were the major subtypes of IgG in both of the two groups. However, the IgG2a level was significantly higher in the A16R group than in the AVA group. At the cellular level, responses of antigen-specific TH2, TH1 and plasma cells were detected. The peripheral TH2 responses could be seen on day 5 after vac- cination, and remained at a high level throughout the experiment (from day 5 post primary immuniza- tion to day 60 post the tertiary immunization); the TH1 responses to A16R vaccine appeared on day 5, while the responses to AVA could only be detected by day 7 after the secondary immunization; a low level of TH1 responses could be observed at the end of the experiment. Antigen-specific plasma cells could be found in the peripheral blood of both the immunized groups, however, the responses in the A16R group appeared earlier, lasted longer, and shown an ascending tendency until the end of the ex- periment when the plasma cell responses in the AVA group were reduced to a very low level. The re- sults suggest that the multiple antigen containing A16R live spore vaccine induces better immune re- sponses than AVA. Combined with serum antibody titers, TH2, TH1 and plasma cell responses could be used as immunological parameters for the evaluation of vaccine efficacy. These findings may afford new insight into the early evaluation of vaccination as well as being a powerful strategy for vacci

关键词： anthrax vaccine adsorbed live spore vaccine antigen secreting cells immunological response ELISPOT

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日本民间追究战争责任运动及其影响

日本学刊 2006年第4期 62-72页

作者：王希亮

上世纪80年代以来,在日本民间出现了追究日本战争责任的市民运动,这对于抵制日本右翼势力的倒行逆施,制约日本政治右倾化倾向,维护亚洲和太平洋地区的和平起到了不可替代的作用。

关键词：市民运动战争责任战争遗留问题