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Parent-offspring relationship recognition based on SSR and mtDNA confirmed resource supplement effect of Fenneropenaeus chinensis release

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Acta Oceanologica Sinica 2024年第2期43卷 156-160页

作者： Song Sun Ding Lyu Xianshi Jin Xiujuan Shan weiji wang Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and *** order to supplement the fishing yield and restore resources of ***,the relevant authorities have carried out the activities of stock enhancement and *** can increase biomass and recover ***,compared with increasing biomass,there were still few reports on its effect on the recovery of *** recovery is a process related to whether the released individuals can form a reproductive *** to now,there has been a lack of evidence whether the released *** can complete the entire life history,and form reproduction *** this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow *** identifying parentage relationships using simple sequence repeat(SSR)and mtDNA haplotype,it was finally confirmed that there were eight released individuals in the recapture *** was confirmed for the first time that at least part of the released *** can complete overwintering and reproductive migration,and maintain the migration habits as their wild ***,we infered that the released shrimp can reproduce under natural conditions,these *** can form reproductive populations theoretically if without human *** results indicated that enhancenment and release activities have a positive effect on resource recovery.

关键词： Fenneropenaeus chinensis release simple sequence repeat(SSR) mtDNA resource supplement

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塔里木盆地深部寒武系复杂地层钻井液技术

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石油钻探技术 2024年第2期52卷 165-173页

作者：金军斌董晓强王伟吉张杜杰国家能源碳酸盐岩油气重点实验室北京102206 中石化石油工程技术研究院有限公司北京102206

塔里木盆地寒武系复杂地层具有超深、超高温、超高压及岩性、流体和应力复杂等地质特征,钻井过程中白云岩破碎性地层垮塌、薄层膏岩溶解导致井壁失稳及钻井液污染、钻井液高温稳定性差等问题突出。基于理论分析,提出了“合理密度支撑、... 详细信息

塔里木盆地寒武系复杂地层具有超深、超高温、超高压及岩性、流体和应力复杂等地质特征,钻井过程中白云岩破碎性地层垮塌、薄层膏岩溶解导致井壁失稳及钻井液污染、钻井液高温稳定性差等问题突出。基于理论分析,提出了“合理密度支撑、随钻多级封堵及封闭浆静态强化”破碎性地层防塌、“欠饱和、低滤失”含膏白云岩地层防卡、“聚合物、磺化材料协同抗温”调控钻井液高温性能的钻井液技术对策。采用抗高温封堵防塌剂、抗高温抗盐聚合物降滤失剂、多尺度宽尺度粒径随钻封堵防塌处理剂、磺化处理剂和抗高温高效润滑剂作为关键处理剂,通过室内试验优选了SMDP-2抗高温抗盐降滤失剂,确定了封堵防塌材料配方和SMJH-1润滑剂最优加量,构建了耐温200℃的抗高温强封堵欠饱和盐水钻井液。该钻井液在QSH1井等3口井进行了应用,表现出良好的抑制性、封堵防塌性和高温稳定性,实现了复杂地层快速钻进。其中,QSH1井下丘里塔格组及阿瓦塔格组井段日平均渗漏量较邻井降低45.6%,井眼扩大率为9.52%,电测、下套管均一次顺利到底,钻井周期缩短30.32%。研究结果表明,该钻井液技术可有效解决温度200℃以内的塔里木盆地寒武系复杂地层钻井液技术难点,为该类地层安全快速钻井提供了技术保障。

关键词：寒武系破碎地层白云岩井壁垮塌欠饱和盐水钻井液塔里木盆地

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聚酰亚胺复合膜的制备及其在沼气提纯中的应用

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天津工业大学学报 2024年第4期43卷 7-11,18页

作者：丁晓莉王巍骥张乾坤张智铭赵红永天津工业大学材料科学与工程学院天津300387 天津工业大学化学工程与技术学院天津300387

为解决沼气分离过程中单级膜过程产物CO_(2)纯度低及CH4的损失率较高等问题,通过界面聚合法制备了具有超高分离性能的具有聚酰亚胺分离层的复合膜,研究了操作温度、进料侧压力及切割比等因素对分离效果的影响。结果表明:该膜适用的温度... 详细信息

为解决沼气分离过程中单级膜过程产物CO_(2)纯度低及CH4的损失率较高等问题,通过界面聚合法制备了具有超高分离性能的具有聚酰亚胺分离层的复合膜,研究了操作温度、进料侧压力及切割比等因素对分离效果的影响。结果表明:该膜适用的温度及压力范围较广,不同操作条件下的膜均表现出了优异的分离性能,产出的CO_(2)纯度高,CH4的损失率低。低温、低压、低切割比有利于渗透侧高纯CO_(2)的输出;高温、高压、低切割比则有利于渗余侧CH4的回收。使用该膜模拟沼气分离,在切割比为0.1下操作,当进气压力为0.2 MPa、温度为25℃时,通过单级膜过程,渗透侧可得到99%的CO_(2),渗余侧CH4回收率可高达99%。在很宽的操作范围内,本文制备的复合膜在沼气中CO_(2)组分分离时,CH4损失率可控制在1%范围内,是理想的CO_(2)/CH4分离膜。

关键词：复合膜二氧化碳捕集聚酰亚胺界面聚合沼气提纯

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Research in migration route of hatchery released Chinese shrimp(Fenneropenaeus chinensis) in the Bohai Bay using method of SSR marker

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Acta Oceanologica Sinica 2020年第12期39卷 76-81页

作者： weiji wang Ding Lyu Mosang wang Kefeng Liu Jie Kong Xiujuan Shan Xianshi Jin Key Laboratory of Sustainable Development of Marine Fisheries of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China Bohai Fisheries Research Institute of Tianjin Tianjin 300457China

This study provides new insights for the hatchery released Chinese shrimp(Fenneropenaeus chinensis),including proportion,dynamic migration route,after they were released into nature for stock enhancement using a new strategy quite different than *** shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in *** 289 sampled individuals during the second voyage,totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR(simple sequence repeats)markers,and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71%in each station with an average value 53.63%,which verified a previous view point that up to 90%of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery ***,the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at midOctober when they initiate over-wintering *** unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring,it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.

关键词： Fenneropenaeus chinensis migration route hatchery release stock enhancement SSR marker

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Estimation of genetic parameters for growth trait of turbot using Bayesian and REML approaches

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Acta Oceanologica Sinica 2017年第6期36卷 47-51页

作者： GUAN Jiantao wang weiji HU Yulong wang Mosang TIAN Tao KONG Jie Ocean University of China Qingdao 266003China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery SciencesQingdao 266071China Shanghai Ocean University Shanghai 201306China

Bayesian and restricted maximum likelihood （REML） approaches were used to estimate the genetic parameters in a cultured turbot Scophthalmus maximus stock. The data set consisted of harvest body weight from 2 462 progenies （17 months old） from 28 families that were produced through artificial insemination using 39 parent fish. An animal model was applied to partition each weight value into a fixed effect, an additive genetic effect, and a residual effect. The average body weight of each family, which was measured at 110 days post-hatching, was considered as a covariate. For Bayesian analysis, heritability and breeding values were estimated using both the posterior mean and mode from the joint posterior conditional distribution. The results revealed that for additive genetic variance, the posterior mean estimate （σa^2 =9 320） was highest but with the smallest residual variance, REML estimates （σa^28 088） came second and the posterior mode estimate （σa^2=7 849） was lowest. The corresponding three heritability estimates followed the same trend as additive genetic variance and they were all high. The Pearson correlations between each pair of the three estimates of breeding values were all high, particularly that between the posterior mean and REML estimates （0.996 9）. These results reveal that the differences between Bayesian and REML methods in terms of estimation of heritability and breeding values were small. This study provides another feasible method of genetic parameter estimation in selective breeding programs of turbot.

关键词： turbot growth traits heritability breeding values REML Bayesian

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Motion control design for unmanned ground vehicle in dynamic environment using intelligent controller

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International Journal of Intelligent Computing and Cybernetics 2017年第4期10卷 530-548页

作者： Auday Almayyahi weiji wang Alaa Adnan Hussein Phil Birch Department of Engineering and Design University of SussexBrightonUK

Purpose–The motion control of unmanned ground vehicles(UGV)is a challenge in the industry of *** purpose of this paper is to propose a fuzzy inference system(FIS)based on sensory information for solving the navigation challenge of UGV in cluttered and dynamic ***/methodology/approach–The representation of the dynamic environment is a key element for the operational field and for the testing of the robotic navigation *** dynamic obstacles move randomly in the operation field,the navigation problem becomes more complicated due to the coordination of the elements for accurate navigation and collision-free path within the environmental *** paper considers the construction of the FIS,which consists of two *** first controller uses three sensors based on the obstacles distances from the front,right and *** second controller employs the angle difference between the heading of the vehicle and the targeted angle to obtain the optimal route based on the environment and reach the desired destination with minimal running power and *** proposed design shows an efficient navigation strategy that overcomes the current navigation challenges in dynamic ***–Experimental analyses are conducted for three different scenarios to investigate the validation and effectiveness of the introduced controllers based on the *** reported simulation results are obtained using MATLAB software *** results show that the controllers of the FIS consistently perform the manoeuvring task and manage the route plan efficiently,even in a complex environment that is populated with dynamic *** paper demonstrates that the destination was reached optimally using the shortest free *** limitations/implications–The paper represents efforts toward building a dynamic environment filled with dynamic obstacles that move at various speeds and *** methodology of designing the FIS is accom

关键词： Fuzzy inference system(FIS) Dynamic obstacles avoidance Intelligent controller Unmanned ground vehicle(UGV)

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An estimation of genetic parameters of growth traits in juvenile turbot (Scophthalmus maximus L.) using parental molecular relatedness

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Acta Oceanologica Sinica 2016年第2期35卷 126-130页

作者： HU Yulong GUAN Jiantao MA Yu KONG Jie wang weiji Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences College of Marine Life Ocean University of China

The estimation of genetic parameters has played an important role in animal selective breeding for growth *** studies show that molecular markers can be incorporated into genetic evaluations. In order to improve the performance of an incomplete pedigree（i.e, only parents are known） in the genetic evaluations, 12 microsatellite markers have been applied in the estimation of the genetic parameters for body weight in a farmed population（n=1 890） of juvenile turbot（Scophthalmus maximus L.）. A new relatedness called parental molecular relatedness（PMR） is estimated based on results of genotyping of 48 parents（31 males, 17 females） with microsatellites markers. The feasibility of PMR in estimation of genetic parameters is verified by comparison with pedigree related（PR） which is obtained from a complete pedigree. The results demonstrate that a high correlation（0.872） between them is found. Heritabilities are estimated using the PMR（0.52±0.13） and PR（0.55±0.22） with the same animal model. A cross-validation shows that the predictive abilities of models using the PMR and the PR are identical（0.81）. From that, a conclusion can be made that PMR and PR predicted genetic values equally well in a population of juvenile turbot. Therefore PMR can be applied as an alternative of the PR when only parents are known. However, for a better performance, more markers and more families should be included in a further study.

关键词： parental molecular relatedness pedigree relatedness turbot genetic parameters

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Estimating genetic parameters with molecular relatedness and pedigree reconstruction for growth traits in early mixed breeding of juvenile turbot

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Acta Oceanologica Sinica 2021年第9期40卷 66-73页

作者： Song Sun weiji wang Yulong Hu Sheng Luan Ding Lyu Jie Kong College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266071China Key Laboratory for Sustainable Development of Marine Fisheries Resources of Ministry of Agriculture Yellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China

An introduced turbot population was used to establish families and to estimate genetic parameters of the offspring. However, there is a lack of pedigree information, and common environmental effects can be introduced when each full-sib family is raised in a single tank. Therefore, in the genetic evaluation, SSRs(simple sequence repeats) were used to reconstruct the pedigree and to calculate molecular relatedness between individuals, and the early mixed-family culture model was used to remove the impact of the common environmental effects. After100 d of early mixed culture, twenty SSRs were used to cluster 20 families and to calculate paired molecular relationships(n=880). Additive genetic matrices were constructed using molecular relatedness(MR) and pedigree reconstruction(PR) and were then applied to the same animal model to estimate genetic parameters. Based on PR, the heritabilities for body weight and body length were 0.214±0.124 and 0.117±0.141, and based on MR they were 0.101±0.031 and 0.102±0.034, respectively. Cross validation showed that the accuracies of the estimated breeding values based on MR(body weight and body length of 0.717±0.045 and 0.629±0.141, respectively) were higher than those of PR(body weight and body length of 0.692±0.052 and 0.615±0.060, respectively). The MR method ensure availability of all genotyped selection candidates, thereby improving the accuracy of the breeding value estimation.

关键词： turbot SSR genetic parameter mixed breeding

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Genetic parameter estimation for juvenile growth and upper thermal tolerance in turbot(Scophthalmus maximus Linnaeus)

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Acta Oceanologica Sinica 2014年第8期33卷 106-110页

作者： ZHANG Tianshi KONG Jie LIU Baosuo wang Qingyin CAO Baoxiang LUAN Sheng wang weiji Key Laboratory for Sustainable Utilization of Marine Fisheries Resources Ministry of AgricultureYellow Sea Fisheries Research InstituteChinese Academy of Fishery Sciences College of Fisheries and Life Science Shanghai Ocean University

Twenty-six half-sib groups （53 full-sib families） of turbot,Scophthalmus maximus Linnaeus, were obtained by artificial insemination. We measured growth in the offspring （40-50 individuals/family） and subjected them to a thermal tolerance challenge over a period of 34 d. There was no significant difference in daily mor-tality （range： 0.580%-1.391%） between Days 1-13 during the thermal tolerance challenge. However, daily cumulative mortality increased rapidly between Days 14 and 29, especially on Days 15 and 16 （20.232% and 34.377%, respectively）. Mortality was highest on Day 16 （14.145%）. We estimated the genetic parameters using the average information restricted maximum likelihood method. We used a likelihood ratio test to evaluate the significance of effects in models with and without identity as an effect, and compared the final log-likelihoods （maximum log L）. Lastly, we estimated phenotypic and genetic correlation between the up-per thermal tolerance limit （UTT） and body weight （BW）. In this study, the positive phenotypic correlation was low between UTT and BW （0.093±0.029）. The genetic correlation between UTT and BW was negative （-0.044±0.239）. The heritability for upper thermal tolerance was low （0.087±0.032）, which is of approximate-ly moderate heritability. The heritability for body weight was high （0.303±0.074）. Our results suggest there is significant potential for improvement in the culture of turbot by selective breeding.

关键词： Scophthalmus maximus Linnaeus growth upper thermal tolerance genetic parameter

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Analysis of Genetic Diversity and Differentiation of Seven Stocks of Litopenaeus vannamei Using Microsatellite Markers

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Journal of Ocean University of China 2014年第4期13卷 647-656页

作者： ZHANG Kai wang weiji LI Weiya ZHANG Quanqi KONG Jie College of Marine Life Sciences Key Laboratory of Marine Genetics and Breeding (Ministry of Education) Ocean University of China Key Laboratory for Sustainable Utilization of Marine Fisheries Resources (Ministry of Agriculture) Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Shandong Marine Fishing and Production Management Station

Seven microsatellite markers were used to evaluate the genetic diversity and differentiation of seven stocks of Litopenaeus vannamei, which were introduced from Central and South America to China. All seven microsatellite loci were polymorphic, with polymorphism information content(PIC) values ranging from 0.593 to 0.952. Totally 92 alleles were identified, and the number of alleles(Na) and effective alleles(Ne) varied between 4 and 21 and 2.7 and 14.6, respectively. Observed heterozygosity(Ho) values were lower than the expected heterozygosity(He) values(0.526–0.754), which indicated that the seven stocks possessed a rich genetic diversity. Thirty-seven tests were detected for reasonable significant deviation from Hardy-Weinberg equilibrium. Fis values were positive at five loci, suggesting that there was a relatively high degree of inbreeding within stocks. Pairwise Fst values ranged from 0.0225 to 0.151, and most of the stock pairs were moderately differentiated. Genetic distance and cluster analysis using UPGMA revealed a close genetic relationship of L. vannamei between Pop2 and Pop3. AMOVA indicated that the genetic variation among stocks(11.3%) was much lower than that within stocks(88.7%). Although the seven stocks had a certain degree of genetic differentiation and a rich genetic diversity, there is an increasing risk of decreased performance due to inbreeding in subsequent generations.

关键词：遗传多样性分析微卫星标记凡纳滨对虾遗传分化 Hardy-Weinberg平衡多态信息含量等位基因近亲繁殖

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