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袖套式包皮环切术联合Z改形治疗包皮过长

组织工程与重建外科杂志 2010年第5期6卷 290-291页

作者：吴春新程开祥刘阳王善良福建省莆田市涵江区医院整形美容科 351111 上海交通大学医学院附属第九人民医院整复外科上海市200011

目的介绍袖套式包皮环切术联合Z改形关闭切口治疗包皮过长与包茎的方法和效果。方法 1%利多卡因阴茎根部浸润麻醉后,根据包皮过长的程度单纯去除相应包皮内、外板的皮肤层,保留完整的肉膜,如果局部存在环形筋膜缩窄环则给予适当松解,缝... 详细信息

目的介绍袖套式包皮环切术联合Z改形关闭切口治疗包皮过长与包茎的方法和效果。方法 1%利多卡因阴茎根部浸润麻醉后,根据包皮过长的程度单纯去除相应包皮内、外板的皮肤层,保留完整的肉膜,如果局部存在环形筋膜缩窄环则给予适当松解,缝合时在切口两侧各作1个Z成形术关闭切口。结果利用该术式治疗包皮过长、包茎20例,术中出血少,术后组织水肿反应轻,并发症少,效果满意。结论袖套式包皮环切术联合切口Z改形关闭切口治疗包皮过长具有手术创伤轻、术后组织水肿轻微,切口愈合快,愈合后皮肤不与白膜粘连或出现切口瘢痕挛缩等优点。

关键词：包皮过长袖套式包皮环切术阴茎筋膜切口Z改形

元宝湾煤矿39102工作面自然发火规律试验研究

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煤炭技术 2024年第9期43卷 162-165页

作者：米会超赵尤信祁云王善良卢金山中煤华昱公司元宝湾煤业山西朔州036999 煤科通安(北京)智控科技有限公司北京100013 煤炭科学技术研究院有限公司北京100013 北京市煤矿安全工程技术研究中心北京100013 智能开采与岩层控制全国重点实验室北京100013 辽宁工业大学机械工程与自动化学院辽宁锦州121001

元宝湾煤矿9煤层具有自燃倾向性,39102工作面在回采时常有CO生成。为了保证矿井安全生产,对煤层自然发火规律进行研究,现场测定39102工作面采空区温度和气体浓度的变化规律,由此划分采空区自燃“三带”。结果表明:不燃带66.6 m;采空区... 详细信息

元宝湾煤矿9煤层具有自燃倾向性,39102工作面在回采时常有CO生成。为了保证矿井安全生产,对煤层自然发火规律进行研究,现场测定39102工作面采空区温度和气体浓度的变化规律,由此划分采空区自燃“三带”。结果表明:不燃带66.6 m;采空区温度在31℃~37℃,未出现自燃;高温范围、CO和CO_(2)高浓度区均出现在自燃带区域内;采空区预防自燃的工作面最低推进速度为60 m/月。测试结果为制定合理的防灭火技术方案提供科学基础参数,保证工作面安全生产。

关键词：自燃倾向性工作面自然发火自燃“三带”

Expression and localization of vitellogenin genes(VTG)and receptor(VGR)in the gonad development of silver pomfret Pampus argenteus

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Journal of Oceanology and Limnology 2023年第4期41卷 1575-1592页

作者： Zitao XIONG Jiazhe YANG Shun ZHANG Yajun wang shanliang XU Chunyang GUO Danli wang School of Marine Sciences Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China

Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus *** qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then *** expression levels of vgr in the testis were low and only 1%-3%of that in *** results show that during the ovarian development of ***,VTG in liver,serum,and ovary all showed a trend from increasing to ***,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA *** VGR content in the testis was extremely low and stable,consistent with vgr *** results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the ***,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the de

关键词： Pampus argenteus vitellogenin(VTG) vitellogenin receptor(VGR) vitellogenesis

葡萄糖氧化酶电极的研制和血清中葡萄糖的测定

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分析化学 1987年第11期 1036-1038页

作者：张国雄刘善良王素芳顾青吴忠华中国科学院上海冶金研究所

葡萄糖氧化酶电极是研究得最多的酶电极之一。本文以牛血清白蛋白-戊二醛为交联剂,将葡萄糖氧化酶固定于醋酸纤维膜上,自制氧电极作电化学敏感元件,研制成葡萄糖氧化酶电极,并用流动注射法分析血清中葡萄糖量,线性响应范围10-6—10-3M,... 详细信息

葡萄糖氧化酶电极是研究得最多的酶电极之一。本文以牛血清白蛋白-戊二醛为交联剂,将葡萄糖氧化酶固定于醋酸纤维膜上,自制氧电极作电化学敏感元件,研制成葡萄糖氧化酶电极,并用流动注射法分析血清中葡萄糖量,线性响应范围10-6—10-3M,每张酶膜可连续测定400次血样,用该电极测定血清中葡萄糖,所得结果与酶光度法一致。

关键词：葡萄糖氧化酶电极醋酸纤维膜氧电极戊二醛胶醛脂肪族醛质控血清己糖葡萄糖右旋糖

Optimal Culture Capacity of White Shrimp (Litopenaeus vannamei) and Razor Clam (Sinonovacula constricta) in a New Series-Connection Culture Model

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Journal of Ocean University of China 2022年第6期21卷 1641-1648页

作者： HU Jiabao ZHAO Chunpu BAO Gege LUO Yunhui wang Danli XU Jilin XU shanliang School of Marine Sciences Ningbo UniversityNingbo 315211China

To construct an effective circular mix-culture model and investigate its culture capacity,we established one small and one large systems,with white shrimp(Litopenaeus vannamei)and razor clam(Sinonovacula constricta)cultured in separate *** culture water from the *** was pumped to the ***,and the culture water from the *** overflowed back into the *** via *** trial I,we tested four culture densities(groups 1–4),and monitored water quality,growth indices,and digestive and immune enzyme *** the results,the nitrogen and phosphorus levels generally increased then declined after 56 days,and were lower in groups 1 and *** specific growth rate of group 2 was the *** 56 days,activities of four digestive enzymes were increased in group 2,and lysozyme activity was significantly decreased in *** in groups 1–4;Alkaline phosphatase activity of *** was increased in group 3,but decreased in *** in groups 2–4;Acid phosphatase activity was significantly higher in groups 1–3(P<0.05),while SOD and CAT activities were significantly elevated in group 2(P<0.05).Thus,we applied group 2 density for trial *** trial II,nitrogen and phosphorus concentrations declined significantly during the latter stages,and growth indices were higher than the control(P<0.05).The yields of *** and *** were significantly higher than the control(P<0.05).The results revealed a good circular mix-culture system with the optimal culture capacity for ***(40 ind m^(−2))and ***(300 ind m^(−2)),which provided a reference for the future culture of them.

关键词： Litopenaeus vannamei Sinonovacula constricta culture capacity series connection culture model water quality

Tbx 4 and Tbx 5 gene expression associated with appendage development and its relationship with the absence of the pelvic fin in Pampus argenteus

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Journal of Oceanology and Limnology 2024年第2期42卷 580-593页

作者： Shun ZHANG Xiaojing ZHU Lingzhu HU Kai LIAO shanliang XU Danli wang Chunyang GUO School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China

The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the *** argenteus is one of the important economical marine fishes widely distributed in offshore ***,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in *** this study,we cloned Tbx4 and Tbx5 cDNA sequence of ***(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of *** addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of *** show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin *** different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult ***,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium.

关键词： Stromateidae pelvic fin protein translation T-box aquaculture broodstock

Numerical prediction of fiber motion in a branching channel flow of fiber suspensions

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Acta Mechanica Sinica 2005年第4期21卷 322-329页

作者： Jianzhong Lin shanliang Zhang James A. Olson Department of Mechanics The State Key Laboratory of Fluid Power Transmission and ControlZhejiang University: Ha ngzhou 310027 China Department of Mechanical Engineering Pulp and Paper CenterThe University of British ColumbiaVancouver BC Canada V6T IZ4

Fiber orientation and dispersion in the dilute fiber suspension that flows through a T-shaped branching channel are simulated numerically based on the slender-body theory. The simulated results are consistent qualitatively with the experimental data available in the literature. The results show that the spatial distribution of fibers is dependent on the fiber aspect ratio, but has no relation with the volume fraction of fiber. The content ratio of fibers near the upper wall increases monotonically with an increasing Re number, and the situation is reverse for the region near the bottom wall. The orientation of fibers depends on Re number, however, the function of fiber volume fraction and aspect ratio is negligible. The fibers near the wall and in the central region of the channel align along the flow direction at all times, but the fibers in the other parts of the channel tend to align along the flow direction only in the downstream region.

关键词： Fiber suspension Distribution Orientation ,Numerical simulation Branching flow

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河北省滦县地区大红峪组沉积特征分析

城市地质 2015年第S1期10卷 252-256页

作者：胡福根王山亮李良景张翊超宋庆伟姚康北京市地质研究所北京100120

河北省滦县地区大红峪组为野鸡坨镇至响棠镇一带中元古界沉积盖层的最底部层位,这与滦县地区在元古宙地处山海关古陆边缘的地理构造格局有关。根据"岩相一层序一沉积环境"的研究思路与方法,将大红峪组地层划分为碎屑岩型和碳酸盐岩型两... 详细信息

河北省滦县地区大红峪组为野鸡坨镇至响棠镇一带中元古界沉积盖层的最底部层位,这与滦县地区在元古宙地处山海关古陆边缘的地理构造格局有关。根据"岩相一层序一沉积环境"的研究思路与方法,将大红峪组地层划分为碎屑岩型和碳酸盐岩型两种相序,两个由TST-HST体系域构成的层序。沉积环境表现为东南侧为山海关古陆,北西侧为浅海台地,沉积作用初期以河流作用为主,进而发展为潮汐作用为主,偶为浅水风暴作用。

关键词：岩相类型层序特征沉积环境大红峪组滦县地区

Rational design of a visible-light photochromic diarylethene:a simple strategy by extending conjugation with electron donating groups

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Science China Chemistry 2019年第4期62卷 451-459页

作者： shanliang Tang Fengling Song Meiheng Lu Keli Han Xiaojun Peng State Key Laboratory of Fine Chemicals Dalian University of Technology State Key Laboratory of Molecular Reaction Dynamics Dalian Institute of Chemical Physics Chinese Academy of Sciences

Photochromic diarylethenes have been widely used in many fields. However, their cyclization process must be induced by UV light. In this article, a simple strategy is developed by extending π-conjugation with electron donating groups. The modified dirylethene derivative can photocyclolize under 405-nm light with a good photochromic efficiency. Meanwhile, its absorption and moderate fluorescence can be switched effectively in both directions by visible lights(405 and 520 nm, respectively) in different solutions and in living cells. We believe that this simple method will become a versatile strategy for developing various dirylethylenes with visible-light photochromism.

关键词： diarylethylenes visible-light photochromism photoreaction quantum yield fluorescence photoswitching