Expression and localization of vitellogenin genes(VTG)and receptor(VGR)in the gonad development of silver pomfret Pampus argenteus

作     者：Zitao XIONG Jiazhe YANG Shun ZHANG Yajun WANG Shanliang XU Chunyang GUO Danli WANG Zitao XIONG;Jiazhe YANG;Shun ZHANG;Yajun WANG;Shanliang XU;Chunyang GUO;Danli WANG

作者机构：School of Marine SciencesNingbo UniversityNingbo 315211China Key Laboratory of Applied Marine BiotechnologyNingbo UniversityChinese Ministry of EducationNingbo 315211China 

出 版 物：《Journal of Oceanology and Limnology》 (海洋湖沼学报（英文）)

年 卷 期：2023年第41卷第4期

页      面：1575-1592页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0707[理学-海洋科学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 090501[农学-动物遗传育种与繁殖] 

基　　金：Supported by the National Natural Science Foundation of China(Nos.31872586,42076118) the Major Project of Science,Technology and Innovation 2025 in Ningbo City(No.2021Z003) the K.C.Wong Magna Fund in Ningbo University 

主　　题：Pampus argenteus vitellogenin(VTG) vitellogenin receptor(VGR) vitellogenesis 

摘      要：Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus *** qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then *** expression levels of vgr in the testis were low and only 1%-3%of that in *** results show that during the ovarian development of ***,VTG in liver,serum,and ovary all showed a trend from increasing to ***,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA *** VGR content in the testis was extremely low and stable,consistent with vgr *** results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the ***,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the de