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A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

Journal of Integrative Agriculture 2024年第3期23卷 923-937页

作者： Sihua Yang Junyi Li Shuai Yang Shiqiao Tang huizhong wang Chunling Xu Hui Xie Laboratory of Plant Nematology andResearch Centerof Nematodes of Plant Quarantine Department of Plant Patholo of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China

In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study,based on transcriptome data, a chorismate mutase gene of Radopholus similis(RsCM) was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity(PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference(RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid(SA) pathway genes(PR1 and PR5) in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

关键词： Radopholus similis chorismate mutase plant defense transgenic tomato

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基于改进YOLOv4算法的遥感图像飞机目标检测

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计算机与数字工程 2024年第2期52卷 416-422页

作者：王惠中文学兰州理工大学电气工程与信息工程学院兰州730050 兰州理工大学甘肃省工业过程先进控制重点实验室兰州730050

针对在遥感图像上对飞机目标检测的精度低问题,论文通过对PANet特征融合网络结构的加深使得YOLOv4算法对小目标的检测更加敏感,进而提高算法的平均检测精度;另外,利用K-means++算法产生了能够自适应与数据集的检测先验框以减少YOLOv4检... 详细信息

针对在遥感图像上对飞机目标检测的精度低问题,论文通过对PANet特征融合网络结构的加深使得YOLOv4算法对小目标的检测更加敏感,进而提高算法的平均检测精度;另外,利用K-means++算法产生了能够自适应与数据集的检测先验框以减少YOLOv4检测算法对边界框回归损失计算过程中的冗余。在RSOD(Remote Sensing Object Detection)数据集上的对比实验表明,综合改进后的YOLOv4算法AP值达到了80.25%。特别地,改进后的YOLOv4算法对小目标检测的置信度得分较高。

关键词：遥感图像目标检测 YOLOv4 特征融合

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基于深度学习的电机故障诊断

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计算机与数字工程 2024年第5期52卷 1536-1540页

作者：王晓兰马泽娟王惠中兰州理工大学兰州730050

故障诊断在保证电机的稳定运行中占据着非常重要的地位,因此,故障诊断在当前的研究中是一个热点。该研究利用短时傅里叶变换把一维的振动信号转换成二维的时频图,进而解决电机轴承的振动信号的非线性和不稳定性问题,并且作为卷积神经网... 详细信息

故障诊断在保证电机的稳定运行中占据着非常重要的地位,因此,故障诊断在当前的研究中是一个热点。该研究利用短时傅里叶变换把一维的振动信号转换成二维的时频图,进而解决电机轴承的振动信号的非线性和不稳定性问题,并且作为卷积神经网络的输入,通过对故障特征信号的直接提取,来形成样本数据集,通过卷积神经网络与softmax多分类器来建立故障诊断模型,在Python中验证该算法优化的准确性,证明了该算法可以提高电机故障诊断的准确率。

关键词：卷积神经网络 softmax多分类器故障诊断短时傅里叶变换

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Microbial community structure and dynamics of starch-fed and glucose-fed chemostats during two years of continuous operation

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Frontiers of Environmental Science & Engineering 2016年第2期10卷 368-380页

作者： Min GOU Jing ZENG huizhong wang Yueqin TANG Toru SHIGEMATSU Shigeru MORIMURA Kenji KIDA College of Architecture and Environment Sichuan University Chengdu 610065 China Department of Food Science Faculty of Applied Life Sciences Niigata University of Pharmacy and Applied Life Sciences (NUPALS) Niigata 956-8603 Japan Graduate School of Science and Technology Kumamoto University Kumamoto 860-0862 Japan

The microbial community structures of two mesophilic anaerobic chemostats, one fed with glucose, the other with starch as sole carbon sources, were studied at various dilution rates （0.05-0.25 d-1 for glucose and 0.025-0.1 d-1 for starch） during two years continuous operation. In the glucose-fed chemostat, the aceticlastic methanogen Methanosaeta spp. and hydrogenotrophic methanogen Methanoculleus spp. predominated at low dilution rates, whereas Methanosaeta spp. and the hydrogenotrophic Methanobacterium spp. predominated together when dilution rates were greater than 0.1 d 1. Bacteria affiliated with the phyla Bacteroidetes, Spiro- chaetes, and Actinobacteria predominated at dilution rates of 0.05, 0.1, and 0.15 d-l, respectively, while Firmicutes predominated at higher dilution rates （0.2 and 0.25 d-l）. In the starch-fed chemostat, the aceticlastic and hydrogeno- trophic methanogens coexisted at all dilution rates. Although bacteria belonging to only two phyla were mainly responsible for starch degradation （Spirochaetes at the dilution rate of 0.08 d-1 and Firmicutes at other dilution rates）, different bacterial genera were identified at different dilution rates. With the exception of Archaea in the glucose-fed chemostat, the band patterns revealed by denaturing gradient gel electrophoresis （DGGE） of the microbial communities in the two chemostats displayed marked changes during long-term operation at a constant dilution rate. The bacterial community changed with changes in the dilution rate, and was erratic during long- term operation in both glucose-fed and starch-fed chemo- stats.

关键词： microbial community glucose degradation,starch degradation dilution rate continuous methanefermentation phylogenetic analysis

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Salt tolerance of transgenic rice (Oryza sativa L.) with mtlD gene and gutD gene

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Chinese Science Bulletin 2000年第18期45卷 1685-1690页

作者： wang huizhong HUANG Danian LU Ruifang LIU Junjun QIAN Qian PENG Xuexian Department of Biology Hangzhou Teacher’s CollegeHangzhou 310012China China National Rice Research Institute Hangzhou 310006China Institute of Microbiology Chinese Academy of SciencesBeijing 100080China

Southern blot analysis indicated that mtID gene (encoding mannitol-1 -phosphate dehydrogenase) and gutD gene (encoding glucitol-6-phosphate dehydrogenase) had been integrated into the rice genome mediated by Agrobacterium tumefaciens LBA4404(pBIGM). The expression of the above two genes in transgenic rice plants was demonstrated by Northern blot analysis and enzymatic activity assay. Analysis of sugar alcohol showed that transgenic rice plants could produce and accumulate mannitol and sorbitol. The salt tolerance of transgenic plants was much higher than that of their controls.

关键词： Oryza sativa L. Agrobacterium tumefaciens D-mannitol-1-phosphate dehydrogenase D-glucitol-6-phosphate dehydrogenase salt tolerance.

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Genome-wide identification, expression analysis of auxin-responsive GH_3 family genes in maize(Zea mays L.) under abiotic stresses

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Journal of Integrative Plant Biology 2015年第9期57卷 783-795页

作者： Shangguo Feng Runqing Yue Sun Tao Yanjun Yang Lei Zhang Mingfeng Xu huizhong wang Chenjia Shen College of Life and Environmental Sciences Hangzhou Normal University Henan Academy of Agricultural Sciences Department of Plant Pathology Washington State University

Auxin is involved in different aspects of plant growth and development by regulating the expression of auxin-responsive family genes. As one of the three major auxin-responsive families, GH3 (Gretchen Hagen3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free indole-3-acetic acid (IAA) to amino acids. However, how GH3 genes function in responses to abiotic stresses and various hormones in maize is largely unknown. Here, the latest updated maize (Zea mays L.) reference genome sequence was used to characterize and analyze the ZmGH3 family genes from maize. The results showed that 13 ZmGH3 genes were mapped on five maize chromosomes (total 10 chromosomes). Highly diversified gene structures and tissue-specific expression patterns suggested the possibility of function diversification for these genes in response to environmental stresses and hormone stimuli. The expression patterns of ZmGH3 genes are responsive to several abiotic stresses (salt, drought and cadmium) and major stress-related hormones (abscisic acid, salicylic acid and jasmonic acid). Various environmental factors suppress auxin free IAA contents in maize roots suggesting that these abiotic stresses and hormones might alter GH3-mediated auxin levels. The respon-siveness of ZmGH3 genes to a wide range of abiotic stresses and stress-related hormones suggested that ZmGH3s are involved in maize tolerance to environmental stresses.

关键词： Abiotic stress auxin auxin responsive gene GH3 gene family maize

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The linkage maps of Dendrobium species based on RAPD and SRAP markers

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Journal of Genetics and Genomics 2010年第3期37卷 197-204页

作者： Dawei Xue Shangguo Feng Hongyan Zhao Hua Jiang Bo Shen Nongnong Shi Jiangjie Lu Junjun Liu huizhong wang College o fLife and Environment Sciences Hangzhou Normal University Hangzhou 310036 China Institute of Plant Protection and Microbiology Zhejiang Academy of Agricultural Science Hangzhou 310021 China

Dendrobium plants are used commonly as tonic herbs and health food in many Asian countries, especially in China. Here we report the genetic map construction of two Dendrobium species with a double pseudo-testcross strategy using random amplified polymorphic DNA （RAPD） and sequence-related amplified polymorphism （SRAP） markers. A F1 mapping population of 90 individuals was developed from a cross between D. officinale and D. hercoglossum. A total of 307 markers, including 209 RAPD and 98 SRAP, were identified and used for genetic linkage group （LG） analysis. The D. officinale linkage map consisted of 11 major linkage groups and 3 doublets, which covered 629.4 cM by a total of 62 markers with an average locus distance of 11.2 cM between two adjacent markers. The D. hercoglos- sum linkage map contained 112 markers mapped on 15 major and 4 minor linkage groups, spanning a total length of 1,304.6 cM with an average distance of I 1.6 cM between two adjacent markers. The maps constructed in this study covered 92.7% and 82.7% of the D. hercoglossum and D. officinale genomes respectively, providing an important basis for the mapping of horticultural and medicinal traits and for the application of marker-assisted selection in Dendrobium breeding program.

关键词： Dendrobium genetic linkage map RAPD SRAP

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食管癌新辅助治疗研究新进展

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食管疾病 2024年第2期6卷 107-114,123页

作者：银嘉鑫王慧中贾国霞全强赵建夫暨南大学第一附属医院广东广州510632

食管癌作为全球常见肿瘤之一,其发病率及死亡率较高,发现时多为中晚期,实现有效治疗目标仍具挑战性。当前新辅助治疗策略,包括化疗、放疗、分子靶向、免疫治疗及综合治疗,在临床中的价值得到广泛认可,但不同方案的选择存在一定争议。本... 详细信息

食管癌作为全球常见肿瘤之一,其发病率及死亡率较高,发现时多为中晚期,实现有效治疗目标仍具挑战性。当前新辅助治疗策略,包括化疗、放疗、分子靶向、免疫治疗及综合治疗,在临床中的价值得到广泛认可,但不同方案的选择存在一定争议。本文结合相关文献对食管癌新辅助治疗方法的研究现状及进展进行了总结和概述,并对其应用前景和存在的问题进行了分析,为进一步优化临床诊疗策略提供参考。

关键词：食管肿瘤新辅助治疗新辅助放化疗新辅助靶向/免疫治疗

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我国退役军人焦虑症状特点及影响因素分析

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职业与健康 2024年第3期40卷 358-364页

作者：李媛媛冯正直廖兴亚赵梦雪王慧中夏凡陆军军医大学研究生院重庆400038 陆军军医大学医学心理系重庆400038 解放军总医院京西医疗区高井门诊部北京100042 解放军第92985部队福建厦门361100

目的分析我国退役军人焦虑症状特点及影响因素。方法采用分层方便抽样,于2021年3月起,历时6个月对我国12个城市2400名退役军人进行问卷调查,调查工具有自编一般情况问卷、广泛性焦虑量表(General anxiety disorder-7,GAD-7),并对数据进... 详细信息

目的分析我国退役军人焦虑症状特点及影响因素。方法采用分层方便抽样,于2021年3月起,历时6个月对我国12个城市2400名退役军人进行问卷调查,调查工具有自编一般情况问卷、广泛性焦虑量表(General anxiety disorder-7,GAD-7),并对数据进行分析。结果我国退役军人焦虑症状总体发生率为30.8%,轻度症状发生率为19.0%,中度症状发生率为5.8%,重度症状发生率为6.0%。人口学因素中,焦虑症状发生率及得分在性别、年龄、居住地、受教育程度、婚姻状况、目前是否患病方面比较,差异均有统计学意义(均P<0.05);军事经历因素中,焦虑症状发生率及得分在服役年限、服役军种、是否特殊环境服役、军事作业类型、立功受奖、服役期间是否患病、退役原因、退役方式方面比较,差异均有统计学意义(均P<0.05)。焦虑症状危险因素为女性、25~<31岁、31~<45岁、职业(农民、商业服务业人员)、目前患重病(均P<0.05),保护因素为较高教育程度(P<0.05)。结论我国退役军人焦虑症状发生率高于现役军人和非军人群体,受到生理状态、目前职业、受教育程度等因素影响。

关键词：退役军人焦虑影响因素

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Hereditary Behavior of bar Gene Cassette is Complex in Rice Mediated by Particle Bombardment

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Journal of Genetics and Genomics 2007年第9期34卷 824-835页

作者：赵艳钱前王慧中黄大年浙江工商大学食品生物与环境工程学院杭州310035 中国水稻研究所水稻生物学国家重点实验室杭州310006 杭州师范学院生命科学学院杭州310036

Particle bombardment transformation using minimal gene cassette （containing the promoter, open reading frame and terminator） is the novel trend in plant genetic transformation, and its use helps to alleviate the undesirable effects of plasmid vector backbone sequences on transgenic plants. In the present article, studies related to the hereditary behavior of bar gene cassette in T1 to T3 generations of the transgenic rice （Oryza sativa L.） lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behaviors including the presence of ‘false homozygotes’, with abnormal segregation ratios ranging from 35：1 to 144：1 （Basta-resistant： sensitive plants） in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny. The homozygotes were obtained in three transgenic lines in T1 generation regardless of the multiple-copy integration patterns of bar gene. Southern blotting analysis showed that multiple copies of bar gene cassette were linked, which formed transgene arrays in the host rice genome. The authors also observed stable transmission of integration patterns of bar gene cassette, as obtained from Southern blotting analysis, in the regularly segregated transgenic rice lines and loss of gene in an irregularly segregated transgenic line. The segregation behavior varied among the transgenic progenies that exhibited similar Southern hybridization patterns of bar gene. On the basis of these results, the multiple-copy integration, gene lost, and gene expres- sion interaction were the major reasons for the complex segregation behaviors of bar gene cassette in transgenic rice plants.

关键词： bar gene cassette particle bombardment hereditary behavior rice

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