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Development of a High-throughput Sequencing Platform for Detection of Viral Encephalitis Pathogens Based on Amplicon Sequencing

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Biomedical and Environmental Sciences 2024年第3期37卷 294-302页

作者： ZHANG Ya Li SU Wen Zhe wang Rui Chen LI Yan ZHANG Jun Feng LIU Sheng Hui HU Dan He XU Chong Xiao YIN Jia yu YIN Qi Kai HE Ying LI Fan FU Shi Hong NIE Kai LIANG Guo Dong TAO Yong XU Song Tao MA Chao Feng wang huan yu Shaanxi University of Chinese Medicine Xianyang 712000ShaanxiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Guangzhou Center for Disease Control and Prevention Guangzhou 510000GuangdongChina Shandong Center for Disease Control and Prevention Jinan 250000ShandongChina Department of Ophthalmology Beijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Xi'an Center for Disease Control and Prevention Xi’an 710054ShaanxiChina

Objective Viral encephalitis is an infectious disease severely affecting human health.It is caused by a wide variety of viral pathogens,including herpes viruses,flaviviruses,enteroviruses,and other viruses.The laboratory diagnosis of viral encephalitis is a worldwide challenge.Recently,high-throughput sequencing technology has provided new tools for diagnosing central nervous system infections.Thus,In this study,we established a multipathogen detection platform for viral encephalitis based on amplicon sequencing.Methods We designed nine pairs of specific polymerase chain reaction(PCR)primers for the 12 viruses by reviewing the relevant literature.The detection ability of the primers was verified by software simulation and the detection of known positive samples.Amplicon sequencing was used to validate the samples,and consistency was compared with Sanger sequencing.Results The results showed that the target sequences of various pathogens were obtained at a coverage depth level greater than 20×,and the sequence lengths were consistent with the sizes of the predicted amplicons.The sequences were verified using the National Center for Biotechnology Information BLAST,and all results were consistent with the results of Sanger sequencing.Conclusion Amplicon-based high-throughput sequencing technology is feasible as a supplementary method for the pathogenic detection of viral encephalitis.It is also a useful tool for the high-volume screening of clinical samples.

关键词： Viral encephalitis Amplicon sequencing High-throughput sequencing Multipathogen detection

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Deliberate self-harm among pediatric psychiatric inpatients in China:A single-center retrospective study

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World Journal of Psychiatry 2024年第3期14卷 398-408页

作者： Xing-Zhi Jiang huan-huan Li Zhen-Zhen yu Chen wang Department of Child and Adolescent Psychiatry Shenzhen Kangning HospitalShenzhen Mental Health CenterShenzhen Key Laboratory for Psychological Healthcare and Shenzhen Institute of Mental HealthShenzhen 518020Guangdong ProvinceChina Department of Psychiatry Xiamen Xianyue HospitalXiamen 361012Fujian ProvinceChina Department of Geriatric Medicine Dalian Medical UniversityDalian 116000Liaoning ProvinceChina Department of Neurology The Second Affiliated Hospital of Xiamen Medical CollegeXiamen 361000Fujian ProvinceChina Department of Neurology and Department of Neuroscience The First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361000Fujian ProvinceChina

BACKGROUND For children and adolescents,deliberate self-harm(DSH)is becoming a mental health problem of concern.Despite several studies on the prevalence and factors of DSH in the world,there is little information on DSH among children and adolescents in China.This study explores the prevalence,types,associated risk factors and tendency of DSH in pediatric psychiatric inpatients in China.AIM To understand the situation of DSH among hospitalized children and adolescents and its related factors.METHODS In this study,we retrospectively studied 1414 hospitalized children and adolescents with mental illness at Xiamen Mental Health Center from 2014 to 2019,extracted the demographic and clinical data of all patients,and analyzed clinical risk factors of DSH.RESULTS A total of 239(16.90%)patients engaged in at least one type of DSH in our study.Cutting(n=115,48.12%)was the most common type of DSH.Females(n=171,71.55%)were more likely to engage in DSH than males(n=68,28.45%).DSH was positively associated with depressive disorders[OR=3.845(2.196-6.732);P<0.01],female[OR=2.536(1.815-3.542);P<0.01],parental marital status[OR=5.387(2.254-12.875);P<0.01]and negative family history of psychiatric illness[OR=7.767(2.952-20.433);P<0.01],but not with occupation,substance use and history of physical abuse.CONCLUSION Our findings suggest that for patients with depression,females,an abnormal marriage of parents,and no history of mental illness,attention should be paid to the occurrence of DSH.

关键词： Deliberate self-harm Children Adolescent Psychiatric inpatients Retrospective study

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Effect of relative humidity on the desulfurization performance of calcium-based desulfurizer

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Journal of Environmental Sciences 2024年第4期138卷 179-188页

作者： Juan Lǖ yu Fu Haiyan yu huanwang Zhe wang Haiyan Chen School of Environment and Resource Southwest University of Science and TechnologyMianyang 621010China Guangyuan Emergency Management bureau Guangyuan 628000China

Low desulfurization efficiency impedes the wide application of dry desulfurization technology,which is a low-cost and simple process,and one significant solution is the development and manufacture of high-performance desulfurizers.In this study,firstly,a steam jet mill was used to digest quicklime;then,we utilized numerical simulation to study the flow field distribution and analyze the driving factors of quicklime digestion;and lastly,the desulfurization performance of the desulfurizer was evaluated under different relative humidities.The results show that the desulfurizer prepared via the steam jet mill had better apparent activity than traditional desulfurizers.Also,the entire jet flow field of the steam jet mill is in a supersonic and highly turbulent flow state,with high crushing intensity and good particle acceleration performance.Sufficient contact with the nascent surface maximizes the formation of slaked lime.The experiments demonstrated that the operating time with 100%desulfurization efficiency and the“break-through”time for the desulfurizer prepared via the steam jet mill is longer than that of traditional desulfurizers,and has significant advantages,especially at low flue gas relative humidity.Compared with traditional desulfurizers,the desulfurizer prepared via steam jet mill expands the range of acceptable flue gas temperature,and the failure temperature is 1.625 times that of traditional desulfurizers.This work breaks through the technical bottleneck of low dry desulfurization efficiency,which is an important step in pushing forward the application of dry desulfurization.

关键词： Dry desulfurization Relative humidity Desulfurization efficiency High-efficiency desulfurizer Quicklime digestion

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Cadaverine and putrescine exposure influence carbon and nitrogen cycling genes in water and sediment of the Yellow River

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Journal of Environmental Sciences 2024年第8期142卷 236-247页

作者： wanghong Su Qiaoling yu Jiawei Yang Qian Han Sijie wang Petr Hedenec Xiaochen wang Ruijun Wan-Yan huan Li School of Public Health Lanzhou UniversityLanzhou 730000China 2 Institute for Tropical Biodiversity and Sustainable DevelopmentUniversity Malaysia Terengganu21030Kuala NerusTerengganuMalaysia State Key Laboratory of Grassland Agro-ecosystems Center for Grassland MicrobiomeCollege of pastoral agriculture science and technology Lanzhou UniversityLanzhou 730000China

The response patterns of microbial functional genes involved in biogeochemical cycles to cadaver decay is a central topic of recent environmental sciences.However,the response mechanisms and pathways of the functional genes associated with the carbon(C)and nitrogen(N)cycling to cadaveric substances such as cadaverine and putrescine remain unclear.This study explored the variation of functional genes associated with C fixation,C degradation and N cycling and their influencing factors under cadaverine,putrescine and mixed treatments.Our results showed only putrescine significantly increased the alpha diversity of C fixation genes,while reducing the alpha diversity of N cycling genes in sediment.For the C cycling,the mixed treatment significantly decreased the total abundance of reductive acetyl-CoA pathway genes(i.e.,acsB and acsE)and lig gene linked to lignin degradation in water,while only significantly increasing the hydroxypropionate-hydroxybutylate cycle(i.e.,accA)gene abundance in sediment.For the N cycling,mixed treatment significantly decreased the abundance of the nitrification(i.e.,amoB),denitrification(i.e.,nirS3)genes in water and the assimilation pathway gene(i.e.,gdhA)in sediment.Environmental factors(i.e.,total carbon and total nitrogen)were all negatively associated with the genes of C and N cycling.Therefore,cadaverine and putrescine exposure may inhibit the pathway in C fixation and N cycling,while promoting C degradation.These findings can offer some new insight for the management of amine pollution caused by animal cadavers.

关键词： Carbon cycling Nitrogen cycling Cadaverine Putrescine Pollution management

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Possible Role of Cellular Polyamine Metabolism in Neuronal Apoptosis

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Current Medical Science 2024年第2期44卷 281-290页

作者： Xin-tong JI Wen-lei yu Meng-jia JINI Lin-jie LU Hong-ping YIN huan-huan wang School of Basic Medical Sciences Affiliated Hospital of Hangzhou Normal UniversityHangzhou Normal UniversityHangzhou 311121China School of Medicine Chongqing UniversityChongqing 400030China Department of Stomatology Huzhou Wuxing District People's HospitalHuzhou Wuxing District Maternal and Child Health HospitalHuzhou 313008China School of Pharmacy Zhejiang UniversityHangzhou 310030 China'Department of Stomatology Haining Hospital of Traditional Chinese MedicineJiaxing 314400China

Recent studies have shown that cellular levels of polyamines(PAs)are significantly altered in neurodegenerative diseases.Evidence from in vivo animal and in vitro cell experiments suggests that the cellular levels of various PAs may play important roles in the central nervous system through the regulation of oxidative stress,mitochondrial metabolism,cellular immunity,and ion channel functions.Dysfunction of PA metabolism related enzymes also contributes to neuronal injury and cognitive impairment in many neurodegenerative diseases.Therefore,in the current work,evidence was collected to determine the possible associations between cellular levels of PAs,and related enzymes and the development of several neurodegenerative diseases,which could provide a new idea for the treatment of neurodegenerative diseases in the future.

关键词： polyamine apoptosis mitochondria neurodegenerative disease

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The Coarse l^(p)-Novikov Conjecture and Banach Spaces with Property(H)

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Chinese Annals of Mathematics,Series B 2024年第2期45卷 193-220页

作者： huan wang Qin wang Research Center for Operator Algebras School of Mathematical SciencesEast China Normal UniversityShanghai 200241China Key Laboratory of MEA Ministry of Educationand Shanghai Key Laboratory of PMMPEast China Normal UniversityShanghai 200241China

In this paper,for 1yu’s Property(H).

关键词： The coarse l^(p)-Novikov conjecture Banach spaces with Property(H) Coarse geometry K-Theory

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Simultaneously enhanced energy storage performance and luminance resistance in (K_(0.5)Na_(0.5))NbO_(3) -based ceramics via synergistic optimization strategy

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Journal of Advanced Ceramics 2024年第1期13卷 34-43页

作者： yu huan Diyu Gui Changxiao Li Tao Wei Lingzhi Wu Xinjian wang Xiaozhi wang Zhenxiang Cheng School of Materials Science and Engineering University of JinanJinan 250022China Yangtze Delta Region Transformation Center for Advanced Technological Achievements Suzhou 215000China Electronic Materials Research Laboratory Key Laboratory of the Ministry of Education and International Center for Dielectric ResearchXi’an Jiaotong UniversityXi’an 710032China Institute for Superconducting and Electronic Materials Australian Institute for Innovative MaterialsUniversity of WollongongInnovation CampusNorth Wollongong 2500Australia

The rapidly advancing energy storage performance of dielectric ceramics capacitors has garnered significant interest for applications in fast charge/discharge and high-power electronic techniques.Exploring the exceptional electrical properties in harsh environments can further promote their practical applications.Defect carriers can be excited under luminance irradiation,thereby leading to degradation of energy storage performance.Herein,a synergic optimization strategy is proposed to enhance energy storage properties and luminance resistance of(K_(0.5)Na_(0.5))NbO_(3)-base(KNN)ceramics.First,the introduction of Bi(Zn0.5Ti0.5)O_(3) solid solution and La3+ions disrupts the long-range polar orders and enhances super paraelectric relaxation characteristics.Additionally,doping La3+ions can increase the band gap and reduce oxygen vacancy concentration,resulting in excellent luminance resistance.Finally,the viscous polymer process is employed to suppress the grain growth and promote chemical homogeneity.As a result,ultrahigh recoverable energy storage density(Wrec)of 8.11 J/cm3 and high efficiency(η)of 80.98%are achieved under an electric field of 568 kV/cm.Moreover,the variations in Wrec andηare only 12.45%and 1.75%,respectively,under 500 W xenon lamp irradiation compared to the performance under a dark environment.These findings hold great potential in facilitating the practical application of dielectric ceramic capacitors in luminance irradiation environments.

关键词： potassium-sodium niobate energy storage performance illumination resistance oxygen vacancy viscous polymer processing

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Large isolated fibrous tumors in the upper esophagus: A case report

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World Journal of Gastrointestinal Oncology 2024年第2期16卷 543-549页

作者： Jia-Jia yu huan-Shuang Pei yu Meng Department of Anesthesiology The Fourth Hospital of Hebei Medical UniversityShijiazhuang 050000Hebei ProvinceChina

BACKGROUND Solitary fibrous tumors(SFT)are rare spindle cell tumors that are usually benign.A total of 10 cases of SFTs in the upper esophagus have ever been reported.Here,we report the anesthetic management of a patient with a large isolated fibrous tumor of the upper esophagus compressing the tracheal membrane.We also provide a literature review of the current research.CASE SUMMARY We report the case of a 49 year old male with“cough aggravation and wheezing after exercise”,who underwent esophagectomy for a large isolated fibrous tumor compressing the tracheal membrane in the upper esophagus.We advise the use of a single-lumen tube with a blocker in patients with difficult airways to reduce the incidence of airway injury and fibrinoscopy at all stages of the perioperative period to guide airway management.This case study is the first report of the anesthetic management of a large,isolated fibrous tumor compressing the tracheal membrane in the upper esophagus.CONCLUSION This rare case emphasizes the importance of perioperative management of anesthesia in patients with large isolated fibrous tumors of the upper esophagus that compress the tracheal membrane.The use of blocker reduce the incidence of airway injury and fibrinoscopy at the perioperative period to guide airway mana-gement.

关键词： Upper esophagus Isolated fibrous tumor Tracheal membrane Peak airway pressure Case report

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Aspartoacylase suppresses prostate cancer progression by blocking LYN activation

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Military Medical Research 2024年第2期11卷 180-205页

作者： Hong Weng Kang-Ping Xiong wang wang Kai-yu Qian Shuai yuan Gang wang Fang yu Jun Luo Meng‑Xin Lu Zhong‑Hua Yang Tao Liu Xing huang Hang Zheng Xing-huan wang Department of Urology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Evidence‑Based and Translational Medicine Zhongnan Hospital of Wuhan UniversityWuhan 430071China Department of Biological Repositories Zhongnan Hospital of Wuhan UniversityWuhan 430071China Hubei Key Laboratory of Tumor Biological Behaviors Wuhan 430071China Department of Pathology Zhongnan Hospital of Wuhan UniversityWuhan 430071China Center for Pathology and Molecular Diagnostics Wuhan UniversityWuhan 430071China Wuhan Research Center for Infectious Diseases and Cancer Chinese Academy of Medical SciencesWuhan 430071China

Background:Globally,despite prostate cancer(PCa)representing second most prevalent malignancy in male,the precise molecular mechanisms implicated in its pathogenesis remain unclear.Consequently,elucidating the key molecular regulators that govern disease progression could substantially contribute to the establishment of novel therapeutic strategies,ultimately advancing the management of PCa.Methods:A total of 49 PCa tissues and 43 adjacent normal tissues were collected from January 2017 to December 2021 at Zhongnan Hospital of Wuhan University.The advanced transcriptomic methodologies were employed to identify differentially expressed mRNAs in PCa.The expression of aspartoacylase(ASPA)in PCa was thoroughly evaluated using quantitative real-time PCR and Western blotting techniques.To elucidate the inhibitory role of ASPA in PCa cell proliferation and metastasis,a comprehensive set of in vitro and in vivo assays were conducted,including orthotopic and tumor-bearing mouse models(n=8 for each group).A combination of experimental approaches,such as Western blotting,luciferase assays,immunoprecipitation assays,mass spectrometry,glutathione S-transferase pulldown experiments,and rescue studies,were employed to investigate the underlying molecular mechanisms of ASPA's action in PCa.The Student‘s t-test was employed to assess the statistical significance between two distinct groups,while one-way analysis of variance was utilized for comparisons involving more than two groups.A two-sided P<0.05 was deemed to indicate statistical significance.Results:ASPA was identified as a novel inhibitor of PCa progression.The expression of ASPA was found to be significantly down-regulated in PCa tissue samples,and its decreased expression was independently associated with patients’prognosis(HR=0.60,95%CI 0.40–0.92,P=0.018).Our experiments demonstrated that modulation of ASPA activity,either through gain-or loss-of-function,led to the suppression or enhancement of PCa cell proliferation,migration,and invasion,respectively.The inhibitory

关键词： Prostate cancer Aspartoacylase LYN JNK AP-1 C-Jun Phosphorylation

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Low profile cavity-embedded ultra-wideband UHF array antenna with end-fire beams

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Journal of Electronic Science and Technology 2024年第2期22卷 24-35页

作者： huan He Wen-Song wang Southwest China Institute of Electronic Technology Chengdu610036China School of Electrical and Electronic Engineering Department Nanyang Technological UniversitySingapore639798Singapore

A low-profile,vertically polarized,ultra-wideband array antenna with end-fire beams operating in an ultra-high frequency(UHF)band is developed in this paper.The array antenna consists of 1×16 log-periodic top-hat loaded monopole antenna arrays and is feasible to embed into a shallow cavity to further reduce the array height.Capacitance is introduced in the proposed antenna element to reduce profile height and the rectangular top hats are carefully designed to minimize the transverse dimension.Simulated results show that when the antenna array operates in a frequency range of 300 MHz-900 MHz,the end-fire radiation pattern achieves±45°scanning range in the horizontal plane.Then prototypes of the proposed end-fire antenna element and a uniformly spaced linear array(1×2)are fabricated and validated.The end-fire antenna array should be suitable for airborne applications where low-profile and conformal scanning phased antenna arrays with end-fire radiations are required.This design is attractive for airborne platform applications that are used to search,discover,identify,and scout the aerial target with vertically polarized beams.

关键词： End-fire radiation Low-profile Vertical polarization Log-periodic array

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