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Biomedical and Environmental Sciences 2011年第4期24卷 364-373页

作者： YAN Wei ZHAO ZhenMin ZHANG LiLi wang dunmei YAN Li YIN NingBei WU Di ZHANG Feng Plastic Surgery Hospital Chinese Academy of Medical Science Peking Union Medical College Beijing 100144 China CAS Key Laboratory of Genome Sciences and Information Beijing Institute of Genomics Chinese Academy of Sciences Beijing 100029 China

Objective Estrogens play an important role in intrinsic skin *** associated changes in global gene expression are poorly *** We used the Illumina microarray platform to obtain comprehensive gene expression profiles in female Chinese Han skin,and confirmed the data by quantitative real-time PCR （Q-RT-PCR）.Results We found 244 genes significantly related to estrogen-associated intrinsic skin aging,and some of these genes were confirmed by *** also performed functional analysis by both Gene Ontology annotation and enrichment of the Kyoto Encyclopedia of Genes and Genomes （KEGG） pathways *** functional analysis revealed 11 biological pathways （including the KEGG pathways,the mitogen-activated protein kinase signaling pathway and metabolic pathways）,that were associated with multiple cellular functions which may be involved in intrinsic skin *** This study suggests that estrogen-associated intrinsic skin aging is a complicated biological process involving many genes and pathways.

关键词： Estrogen Intrinsic aging Microarray Metabolism

Robust Timing Constraints for Granitic Magmatism and Hydrothermal Mineralization in the Tieshanlong W-Sn Ore Field,Eastern Nanling Range,South China

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Acta Geologica Sinica(English Edition) 2024年第5期98卷 1255-1269页

作者： LI Wei TANG Juxing GUO Na LANG Xinghai SONG Shiwei WU Zhongru PENG Linlin FANG Xiang CHEN Binfeng LIAN dunmei LU Jie The Seventh Geological Brigade of Jiangxi Bureau of Geology Key Laboratory of Ionic Rare Earth Resources and EnvironmentGanzhouJiangxi 341000China College of Earth and Planetary Sciences Chengdu University of TechnologyChengdu 610059China Jiangxi Province Key Laboratory of Exploration and Development of Critical Mineral Resources Nanchang 330036China MNR Key Laboratory of Metallogeny and Mineral Resource Assessment Institute of Mineral ResourcesCAGSBeijing 100037China Institute of Earth Sciences China University of GeosciencesBeijing 100083China Jiangxi Geological Bureau Nanchang 330036China Chinese Academy of Geological Sciences Beijing 100037China

The Tieshanlong ore field is an important part of the Nanling Range,which is famous worldwide for its W-Sn ***,the mineralization age of the Tieshanlong ore field is not well constrained,and our field investigation reveals that granitic emplacement occurred at different ***,previous studies have not distinguished these multiple stages of *** Tieshanlong granite complex is closely related to the Huangsha quartz vein-type W-Sn deposit and Tongling skarn-type Cu-W-Sn deposit in this *** field investigations and isotopic age analyses,this work studies the relationship between multistage magmatic activity and mineralization in the Tieshanlong ore ***-ICP-MS zircon U-Pb isotope analyses revealed that the first-and second-staged granites formed at 154.2±0.6 Ma(MSDW=1.4)and 151.2±0.4 Ma(MSDW=1.5),with zirconε_(Hf)(t)values ranging from-13.1 to-10.5 and from-14.7 to-11.1,*** data suggest that the Tieshanlong granite complex was derived from the partial melting of ancient crustal ***-ICP-MS U-Pb dating of wolframite and cassiterite reveals that W-Sn mineralization occurred at 160-150 Ma,which agrees well with the U-Pb dating results of the second-staged granite within analytical *** magmatic activity in this ore field can be divided into three stages:175-154 Ma,154-150 Ma and 150-145 *** quartz vein-and skarn-type W-Sn mineralization is closely related to second-staged fine-grained twomica granite,and formed earlier than skarn-type *** study establishes a metallogenic model for the Tieshanlong ore field,and this model has important practical significance for identifying concealed W-Sn(-Cu)deposits around other granitic complexes in the Nanling Range.

关键词： zircon U-Pb-Hf isotopes wolframite U-Pb ages cassiterite U-Pb ages metallogenic model Tieshanlong ore field Nanling Range

An Analysis of the Lack of Modern Educational Technology in the Teaching of Secondary Vocational English Subjects and its Teaching Strategies--Take Pingli Vocational Education Center as an Example

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Journal of Contemporary Educational Research 2021年第2期5卷 1-3页

作者： dunmei Yuan Ping Li Vocational Education Centre China

The report of the Educational sector in China advocates modern education technology,and now a new round of curriculum reform has thoroughly revised the curriculum standards of middle school students in various subjects,and penetrated modern educational technology into the teaching of various *** recent years,the education department has raised the importance of vocational education,changed the concept of education for teachers and students,vocational education is in the fastest-growing period,the majority of secondary vocational education students has greatly improved,the curriculum is *** should make good use of all kinds of the up-to-dated educational technologies in various ***,in the process of education,the basic level of secondary vocational students is relatively poor phenomenon slowly appeared,which is a common problem in secondary vocational education,so we should strengthen the modern education technology in English subjects of the value of ***,like our mother tongue,has a wealth of humanistic knowledge and spiritual *** English teachers in secondary vocational school simply teach basic English knowledge aspects and English *** only lets the most vocational students to learn only the most basic English textbook knowledge,and then English teachers find many bad aspects,such as:students do not understand foreign culture;Therefore,secondary vocational English teachers in the English classroom to penetrate modern education technology,is the most important ***,as an English teacher,how to modernize secondary school students in classroom teaching?This article mainly talks about the teaching of secondary English subjects lack modern education technology and its teaching strategies.

关键词： modern educational technology seco-ndary vocational English students English teachers missing analysis

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高等植物传粉方式的多样性

生物学教学 2007年第12期32卷 8-9页

作者：林敦梅华中师范大学生命科学学院武汉430079

传粉是高等植物受精的必经阶段,高等植物在漫长的演化过程中形成了多种多样的适应自身生存环境的传粉方式,主要有生物传粉、非生物传粉以及一些特殊的传粉方式。

关键词：高等植物传粉方式多样性

缙云山亚热带森林林下常见蕨类叶与细根分解碳氮磷释放动态

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科学通报 2019年第23期64卷 2430-2440页

作者：杨术芳豆鹏鹏王红娟王芳杨光蓉林敦梅重庆大学三峡库区生态环境教育部重点实验室重庆400045 重庆市农业科学院生物技术研究中心重庆401329

凋落物分解是森林碳和养分循环的关键环节.长期以来森林凋落物分解研究主要关注森林优势树种.虽然蕨类植物是亚热带森林林下层的重要组分,占林下层大量的生物量,但其凋落物分解过程很少被关注.本研究用缙云山亚热带常绿阔叶林林下12种... 详细信息

凋落物分解是森林碳和养分循环的关键环节.长期以来森林凋落物分解研究主要关注森林优势树种.虽然蕨类植物是亚热带森林林下层的重要组分,占林下层大量的生物量,但其凋落物分解过程很少被关注.本研究用缙云山亚热带常绿阔叶林林下12种常见的蕨类叶和细根进行分解实验,监测分解113, 198, 386和586 d后的碳氮磷释放动态.结果表明:蕨类叶与细根初始碳含量无显著差异,但叶的初始氮磷含量均显著高于细根.多数物种叶的碳氮磷释放速率显著快于细根,且叶的碳氮磷主要表现为简单的直接释放模式,而细根的碳氮磷释放则表现出直接释放、富集-释放、富集-释放-富集、始终富集等复杂模式.氮、磷残余率随碳残余率的变化格局分别受初始氮、磷含量的影响.叶与细根间碳氮磷残余率的相关性表现出不同的格局:碳残余率在分解113, 198和386 d后均为显著正相关,但586 d后关系不显著;而磷残余率除在113 d时关系不显著外在其他时间点均显著正相关;但氮残余率在整个分解过程均无显著关系.该结果表明地上地下分解速率是否存在显著相关性与元素类型及分解时间有关.本研究结果为进一步量化蕨类植物对森林生态系统碳和养分循环的重要性提供参考.

关键词：蕨类植物叶分解细根分解养分释放化学计量学地上地下协变

重庆市主城区22种常见绿化树种凋落叶的持水特性

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中国城市林业 2021年第6期19卷 15-20页

作者：王芳林敦梅重庆大学三峡库区生态环境教育部重点实验室重庆400044

文章以重庆市主城区22个常见绿化树种为研究对象,通过蒸馏水浸泡法测定凋落叶的持水特性;再通过测定不同树种凋落叶的性状,分析其与凋落叶持水特性之间的关系。结果表明:随着浸泡时间的增加,所有树种凋落叶的吸水量呈对数增加;不同树种... 详细信息

文章以重庆市主城区22个常见绿化树种为研究对象,通过蒸馏水浸泡法测定凋落叶的持水特性;再通过测定不同树种凋落叶的性状,分析其与凋落叶持水特性之间的关系。结果表明:随着浸泡时间的增加,所有树种凋落叶的吸水量呈对数增加;不同树种凋落叶的标准持水力(浸泡1 h)和最大持水力(浸泡24 h)均存在显著差异(P<0.001),其中黄葛树的标准持水力最大(90.5±2.1)%,鸳鸯茉莉的最大持水力最大(268.0±22.5)%,而蚊母树的标准持水力(5.8±0.4)%和最大持水力(21.3±2.3)%均最小;标准持水力和最大持水力均与叶干物质含量呈显著负相关关系,最大持水力还与比叶面积呈显著正相关关系,说明叶的性状可以预测其凋落叶的持水力特性。基于研究结果,在城市绿地中选择种植鸳鸯茉莉、红背桂、金丝桃、栀子花等灌木,以及黄葛树、广玉兰等乔木将更有利于提高城市森林凋落物层的水源涵养能力。研究结果可为提升城市绿地水源涵养功能的树种选择提供参考。

关键词：绿化树种凋落叶叶片性状持水特性水源涵养

Targeted bisulfite sequencing identified a panel of DNA methylation-based biomarkers for esophageal squamous cell carcinoma(ESCC)

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Targeted bisulfite sequencing identified a panel of DNA meth...

Identification of hyper-methylated tumor suppressor genes-based diagnostic panel for esophageal squamous cell carcinoma(ESCC) in a Chinese Han population

2017中国长三角遗传学大会

作者： Weilin Pu Chenji wang Sidi Chen dunmei Zhao Yinghui Zhou Yanyun Ma Ying wang Caihua Li Zebin Huang Li Jin Shicheng Guo Jiucun wang Minghua wang State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life Sciences and Institutes of Biomedical SciencesFudan University Department of Biochemistry and Molecular Biology Medical CollegeSoochow University Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan University

Background:DNA methylation has been implicated as a promising biomarker for precise cancer ***,limited DNA methylation-based biomarkers have been described in esophageal squamous cell carcinoma(ESCC).Method:A high-throughput DNA methylation dataset(100 samples) of ESCC from the cancer genome atlas(TCGA) project was analyzed and validated along with another independent dataset(12 samples) from the gene expression omnibus(GEO) *** methylation status of peripheral blood mononuclear cells and peripheral blood leucocytes from healthy controls was also utilized for biomarker *** candidate CpG sites as well as their adjacent regions were further validated in 94 pairs of ESCC tumor and adjacent normal tissues from the Chinese Han population using the targeted bisulfite sequencing *** regression and several machine learning methods were applied for evaluation of the diagnostic ability of our *** the discovery stage,five CpG sites were selected as candidate biomarkers for further analysis as shown below:cg05249644,P=2.20 × 10;cgl5830431,P = 3.60 × 10;cg20655070,P = 3.60 × 10;cg26671652,P = 5.77 × 10;and cg27062795,P = 3.60 × *** the validation stage,the methylation status of both the five CpG sites and their adjacent genomic regions were *** diagnostic model based on the combination of these five genomic regions yielded a robust performance(Sensitivity = 0.75,Specificity = 0.88,AUC = 0.85).Eight statistical models along with five-fold cross-validation were further applied,in which the SVM model reached the best accuracy in both training and test dataset(Accuracy = 0.82 and 0.80,respectively).In addition,subgroup analyses revealed a significant difference in diagnostic performance between the alcohol use and non-alcohol use ***:Methylation profiles of the five genomic regions covering cg05249644(STK3),cgl5830431,cg20655070,cg26671652(ZNF418) and cg27062795(ZNF542) can be used for effective met

关键词： Esophageal squamous cell carcinoma DNA methylation Biomarker Diagnosis Targeted bisulfite sequencing

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Identification of hyper-methylated tumor suppressor genes-ba...

金佛山亚热带常绿阔叶林地表土壤动物群落特征及其影响因素

2017中国长三角遗传学大会

作者： Chenji wang Weilin Pu dunmei Zhao Sidi Chen Yinghui Zhou Zhenglei He Xulong Feng Ying wang Caihua Li Li Jin Shicheng Guo Jiucun wang Minghua wang Department of Biochemistry and Molecular Biology Medical CollegeSoochow University State Key Laboratory of Genetic Engineering Collaborative Innovation Center for Genetics and DevelopmentSchool of Life SciencesFudan University Ministry of Education Key Laboratory of Contemporary Anthropology School of Life SciencesFudan University

Background:DNA methylation-based biomarkers were suggested to be promising for early cancer ***,DNA methylation-based biomarkers for esophageal squamous cell carcinoma(ESCC),especially in Chinese Han populations have not been identified and evaluated ***:Candidate tumor suppressor genes(N=65) were selected through literature searching and four public high-throughput DNA methylation microarray datasets including 136 samples totally were collected for initial *** bisulfite sequencing was applied in an independent cohort of 94 pairs of ESCC and normal tissues from a Chinese Han population for eventual ***:ADHFE1,EOMES,SALL1 and TFPI2 were identified and validated in the ESCC samples from a Chinese Han *** four candidate regions were validated to be significantly hypermethylated in ESCC samples(ADHFE1,p = 1.70×10;EOMES,p = 2.90×10;SALLl,p = 3.90×10;TFPI2,p = 3.40 × 10).Logistic regression based prediction model shown a robust ESCC classification performance(Sensitivity = 66%,Specificity = 87%,AUC = 0.81).Moreover,advanced classification method had better performances(random forest and naive Bayes).Interestingly,the diagnostic performance could be improved in non-alcohol use subgroup(AUC = 0.84).Conclusion:Methylation panels of ADHFEl,EOMES,SALL1 and TFPI2 could be an effective methylation-based assay for ESCC diagnosis.

关键词： Esophageal squamous cell carcinoma DNA methylation Biomarker Diagnosis Targeted bisulfite sequencing

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生态学报 2020年第21期40卷 7602-7610页

作者：杨光蓉豆鹏鹏马瑜王红娟林敦梅重庆大学三峡库区生态环境教育部重点实验室重庆400045 重庆市农业科学院生物技术研究中心重庆401329

为探讨小尺度下不同微生境的土壤动物群落特征及其与环境因子之间的关系,于2018年10月在金佛山西坡亚热带常绿阔叶林样带内,对其凋落物层及腐殖质层两类微生境的土壤动物群落进行调查及相应环境因子的测定。此次调查共捕获地表土壤动物1... 详细信息

为探讨小尺度下不同微生境的土壤动物群落特征及其与环境因子之间的关系,于2018年10月在金佛山西坡亚热带常绿阔叶林样带内,对其凋落物层及腐殖质层两类微生境的土壤动物群落进行调查及相应环境因子的测定。此次调查共捕获地表土壤动物12381头,隶属于3门9纲22目。其中优势类群为蜱螨目和长角虫兆目,个体数占比为75.24%;常见类群为原虫兆目、愈腹虫兆目、短角虫兆目、双翅目、鞘翅目和膜翅目,个体数占比为21.23%。同时,土壤动物的密度(M)、Shannon-Wiener多样性指数(H)、Simpson优势度指数(D)及Pielou均匀度指数(E)均表现为腐殖质层极显著高于凋落物层(P<0.01)。根据回归分析及冗余分析结果发现,两类微生境的土壤动物群落特征与环境因子的关系存在一定差异;影响凋落物层土壤动物群落特征的重要环境因子为凋落物的总有机碳、碳氮比、湿度及pH,而影响腐殖质层土壤动物群落特征的重要环境因子为腐殖质的干重、总氮、总磷、湿度、pH及微生物生物量氮。研究表明,常绿阔叶林生态系统的不同微生境间土壤动物多样性特征存在显著差异,小尺度下环境因子对土壤动物群落特征具有重要影响。

关键词：土壤动物群落特征环境因子小尺度金佛山

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肝硬化与胰岛素抵抗关系

中国交通医学杂志 2005年第3期19卷 225-226页

作者：赵敦美如皋市薛窑医院江苏226541

目的:进一步了解肝性糖尿病发病原因和特点。方法:测定18例肝硬化患者(肝硬化组)及23例健康人(对照组)空腹胰岛素浓度,计算胰岛素敏感性指数。结果:对照组FPG(5.25±0.04)mmol/L,FINS(7.3±4.4)mU/L,胰岛素敏感性指数-1.37±0.01;肝硬化... 详细信息

目的:进一步了解肝性糖尿病发病原因和特点。方法:测定18例肝硬化患者(肝硬化组)及23例健康人(对照组)空腹胰岛素浓度,计算胰岛素敏感性指数。结果:对照组FPG(5.25±0.04)mmol/L,FINS(7.3±4.4)mU/L,胰岛素敏感性指数-1.37±0.01;肝硬化组FPG(5.37±0.06)mmol/L,FINS(16.2±13.6)mU/L,胰岛素敏感性指数-1.61±0.05。结论:肝硬化患者存在高胰岛素血症及胰岛素抵抗,这可能是肝性糖尿病的发病原因。

关键词：肝性糖尿病肝硬化胰岛素抵抗