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Optimization of Transformation Efficiency of Suspension Cultured Vitis vinifera *** Embryogenic Cells

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Journal of Integrative Agriculture 2012年第3期11卷 387-396页

作者： WU Jiao ZHANG Ya-li HE Rong-rong ZHU Lei wang chao-xia XU xia LU Jiang College of Food Science and Nutritional Engineering China Agricultural University Beijing 100083 P.R. China Center for Viticulture and Small Fruit Research Florida A&M University Tallahassee 32317 USA

Vitis vinifera *** suspension cultures were established from proembryogenic mass and employed for optimizing Agrobacterium-mediated transformation ***-factor-at-a-time experiment revealed that OD600 of Agrobacterium,time of inoculation,co-cultivation,and cell-drying before inoculation significantly affected the transformation efficiency which reached maximum 21.5% at the following conditions：0.8 of OD600,25 min of inoculation,2 d of co-cultivation,and 10 min of cell *** surface methodology experiments based on a five-level,four-factor central-composite rotatable design were then used to optimize these selected *** optimized conditions for Chardonnay grape transformation were：0.8711 of OD600,28.9 min of inoculation,2.25 d of co-cultivation and 11.76 min of cell *** optimization,transformation efficiency was 26.2% and there were no interactions among different factors.

关键词： transformation Chardonnay suspension cultures response surface methodology

Cistanche deserticola decoction alleviates the testicular toxicity induced by hydroxyurea in male mice

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Asian Journal of Andrology 2013年第6期15卷 838-840,I0011页

作者： Li Gu Wen-Ting Xiong chao wang Hong-xia Sun Guo-Fu Li Xin Liu Food and Health Engineering Research Center of State Education Ministry Sun Yat-Sen University Guangzhou 510275 China

This study aimed to evaluate testicular toxicity induced by hydroxyurea （HU） and the possible counteracting effect of an aqueous extract of Cistanche deserticola （CD）. HU is an antineoplastic drug that has potential reproductive toxicity, and Herba Cistanche has been used as a tonic for the reproductive system for thousands of years. Sixty mice were randomly divided into five groups. Except mice in normal group, the rest received HU （400 mg kg^-1 body weight） intragastrically. Meanwhile, mice in normal and HU control groups received purified water, and the rest received intragastrically three doses of CD decoctions （1.5, 3.0 and 6.0 g crude drug kg^-1 body weight, respectively） daily for 4 weeks. Severe testes lesions were observed, testes weight （P〈0.01） and serum luteinising hormone levels （P〈0.0 1） were also decreased significantly, in the HU groups. Three doses of CD decoctions alleviated the spermatogenetic cell degeneration induced by HU and modulated the serum sex hormones levels to some extent.

关键词： Cistanche deserticola hormones hydroxyurea semin iferous tubule

Effect of mechanical stresses on degradation behavior of high-purity magnesium in bone environments

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Journal of Materials Science & Technology 2024年第4期171卷 252-261页

作者： Yan Yao Jie xia Lizhen wang Yuanming Gao Xili Ding chao wang Yubo Fan Key Laboratory of Biomechanics and Mechanobiology(Beihang University) Ministry of EducationBeijing Advanced Innovation Center for Biomedical EngineeringSchool of Biological Science and Medical EngineeringSchool of Engineering MedicineBeihang UniversityBeijing 100083China

High-purity(HP)magnesium(Mg)has emerged as a promising biomaterial for supporting functional bone *** previous study found that mechanical stresses and the surrounding fibrotic tissue(subcuta-neous)both play crucial roles in the degradation of HP ***,due to challenges in the degradation and regeneration process in vivo,it remains unclear how stress affects HP Mg degradation in bone en-vironments,limiting its further *** this study,novel loading devices were designed and the effects of tensile and compressive stresses on HP Mg degradation in vivo and in vitro bone environments were quantitatively *** addition,bone osteointegration around HP Mg was explored *** stress increases the degradation rate of HP Mg in vivo and in *** Mg degradation in vivo is more sensitive to stress factors than in vitro,but the sensitivity decreases with corrosion *** volume loss rate of HP Mg is multilinear with the applied stress and degradation *** volume of bone tissue surrounding HP Mg is larger in the no-stress group compared to the stressed groups,which is more pronounced with increasing implantation *** results provide valuable insights for optimiz-ing the design of HP Mg-based implants considering load *** will help to achieve a balance between the degradation rate of the implant and the regeneration rate of the surrounding bone.

关键词： High-purity magnesium Quantitative stresses Degradation Bone environments In vivo and in vitro

A new fluorocarbon adhesive:Inhibiting agglomeration during combustion of propellant via efficient F-Al_(2)O_(3) preignition reaction

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Carbon Energy 2024年第6期6卷 292-305页

作者： Qifa Yao Min xia chao wang Fanzhi Yang Wei Yang Yunjun Luo School of Materials Science and Engineering Beijing Institute of TechnologyBeijingChina Key Laboratory of High Energy Density Materials Ministry of EducationBeijing Institute of TechnologyBeijingChina School of Aerospace Engineering Beijing Institute of TechnologyBeijingChina Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijingChina

Inhibiting the agglomeration of molten aluminum particles packed in the binder network is a promising scheme to achieve efficient combustion of solid *** this investigation,the hydroxyl-terminated structured fluorinated alcohol compound(PFD)was introduced to modify the traditional polyethylene glycol/polytetrahydrofuran block copolymerization(HTPE)binder;that is,a unique fluorinated polyether(FTPE)binder was synthesized by embedding fluorinated organic segments into the HTPE binder via crosslinking *** FTPE was applied in aluminum-based propellants for the first *** to the complete release of fluorinated organic active segments in the range of 300℃to 400℃,the burning rate of FTPEbased propellant increased from 4.07(0%PFD)to 6.36 mm/s(5%PFD),increased by 56.27%under 1 *** reaction heat of FTPE propellants increased from 5.95(0%PFD)to 7.18 MJ/kg(5%PFD)under 3.0 MPa,indicating that HTPE binder modified with PFD would be conducive to inhibiting the D90 of condensed combustion products(CCPs)dropped by 81.84%from 75.46(0%PFD)to 13.71μm(5%PFD)under 3.0 MPa,in consistent with the significant reduction of aluminum agglomerates observed on the quenched burning surface of the *** results demonstrated that a novel FTPE binder with PFD can release fluorinated organic active segments,which motivate preignition reaction with the alumina shell in the early stage of aluminum combustion,and then enhance the melting diffusion effect of aluminum to inhibit the agglomeration.

关键词： agglomeration characteristics aluminum particles combustion fluorine alcohol compounds HTPE propellants

基于循证理论的营养管理在宫颈癌放疗患者中的应用

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海南医学 2024年第10期35卷 1494-1498页

作者：宋方方祝宾华王朝霞李俊灵顾浩郑州大学第一附属医院放疗科河南郑州450052

目的探讨基于循证理论的营养管理在宫颈癌放疗患者中的应用效果。方法选取2021年1月至2023年4月于郑州大学第一附属医院就诊的134例宫颈癌放疗患者纳入研究,按随机数表法分为循证营养组和常规组各67例。放疗期间,常规组患者给予常规的... 详细信息

目的探讨基于循证理论的营养管理在宫颈癌放疗患者中的应用效果。方法选取2021年1月至2023年4月于郑州大学第一附属医院就诊的134例宫颈癌放疗患者纳入研究,按随机数表法分为循证营养组和常规组各67例。放疗期间,常规组患者给予常规的护理干预,循证营养组患者在常规护理的基础上给予基于循证理论的营养管理。放疗结束后(干预后)比较两组患者的营养状态[转铁蛋白(TRF)、白蛋白(ALB)、血红蛋白(Hb)]、心理状态[焦虑自评量表(SAS)、抑郁自评量表(SDS)]和生存质量[生活质量测定核心量表(QLQ-C30)],同时比较两组患者干预期间的不良反应及放疗耐受性。结果干预后,循证营养组患者的血清TRF、ALB、Hb水平分别为(2.07±0.14)g/L、(33.79±1.03)g/L、(119.25±1.14)g/L,明显高于常规组的(1.78±0.11)g/L、(28.63±0.94)g/L、(103.71±1.09)g/L,差异均有统计学意义(P<0.05);干预后,循证营养组患者的SAS评分、SDS评分分别为(47.21±1.49)分、(45.85±2.28)分,明显低于常规组的(59.85±5.33)分、(54.36±3.15)分,差异均有统计学意义(P<0.05);干预后,循证营养组患者的躯体、社会、认知、情绪功能评分分别为(90.15±1.05)分、(70.35±3.51)分、(92.03±1.43)分、(87.42±3.35)分,明显高于常规组的(85.42±1.01)分、(64.89±2.86)分、(87.14±1.27)分、(78.14±2.52)分,差异均有统计学意义(P<0.05);循证营养组患者的骨髓抑制、急性放射性直肠炎、急性胃肠道反应发生率分别为70.15%、52.24%、76.12%,明显低于常规组的85.07%、73.13%、89.55%,差异均有统计学意义(P<0.05);循证营养组患者放疗中断率为0,明显低于常规组的8.96%,差异有统计学意义(P<0.05)。结论基于循证理论的营养管理可改善宫颈癌放疗患者的营养状态,调节其情绪状态,提升其放疗耐受性,降低不良反应的发生率,从而提高生存质量。

关键词：宫颈癌循证理论营养管理放疗营养状态生存质量

FERONIA-mediated TIR1/AFB2 oxidation stimulates auxin signaling in Arabidopsis

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Molecular Plant 2024年第5期17卷 772-787页

作者： Baiyan Lu Shengnan wang Hanqian Feng Jing wang Kaixing Zhang Yilin Li Ping Wu Minmin Zhang Yanshu xia chao Peng chao Li School of Life Sciences East China Normal UniversityShanghai 200241China National Facilty for Protein Science Shanghai Zhangjang LabShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China

The phytohormone auxin plays a pivotal role in governing plant growth and *** the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX(TIR1/AFB)receptors function in both the nucleus and cytoplasm,the mechanism governing the distribution of TIR1/AFBs between these cellular compartments remains *** this study,we demonstrate that auxin-mediated oxidation of TIR1/AFB2 is essential for their targeting to the *** showed that small active molecules,reactive oxygen species(ROS)and nitric oxide(NO),are indispensable for the nucleo-cytoplasmic distribution of TIR1/AFB2 in trichoblasts and root *** studies revealed that this process is regulated by the FERONIA receptor kinase–NADPH oxidase signaling ***,ROS and NO initiate oxidative modifications in TIR1C140/516 and AFB2C135/511,facilitating their subsequent nuclear *** oxidized forms of TIR1C140/516 and AFB2C135/511 play a crucial role in enhancing the function of TIR1 and AFB2 in transcriptional auxin ***,our study reveals a novel mechanism by which auxin stimulates the transport of TIR1/AFB2 from the cytoplasm to the nucleus,orchestrated by the FERONIA–ROS signaling pathway.

关键词： FERONIA TIR1/AFBs oxidative modification nucleo-cytoplasmic distribution root hair development

Development of advanced anion exchange membrane from the view of the performance of water electrolysis cell

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Journal of Energy Chemistry 2024年第3期90卷 348-369,I0009页

作者： chao Liu Zhen Geng Xukang wang Wendong Liu Yuwei wang Qihan xia Wenbo Li Liming Jin Cunman Zhang Clean Energy Automotive Engineering Center Tongji UniversityShanghai 201804China School of Automotive Studies Tongji UniversityShanghai 201804China

Green hydrogen produced by water electrolysis combined with renewable energy is a promising alternative to fossil fuels due to its high energy density with zero-carbon *** water electrolysis technologies,the anion exchange membrane(AEM) water electrolysis has gained intensive attention and is considered as the next-generation emerging technology due to its potential advantages,such as the use of low-cost non-noble metal catalysts,the relatively mature stack assembly process,***,the AEM water electrolyzer is still in the early development stage of the kW-level stack,which is mainly attributed to severe performance decay caused by the core component,i.e.,***,the review comprehensively presents the recent progress of advanced AEM from the view of the performance of water electrolysis ***,fundamental principles and critical components of AEM water electrolyzers are introduced,and work conditions of AEM water electrolyzers and AEM performance improvement strategies are *** challenges and perspectives are also analyzed.

关键词： Hydrogen Water electrolysis Anion exchange membrane Electrolysis cell

p38 MAPK inhibitor SB202190 suppresses ferroptosis in the glutamate-induced retinal excitotoxicity glaucoma model

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Neural Regeneration Research 2024年第10期19卷 2299-2309页

作者： Lemeng Feng chao wang Cheng Zhang Wulong Zhang Weiming Zhu Ye He Zhaohua xia Weitao Song National Clinical Research Center for Geriatric Diseases Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina Eye Center of xiangya Hospital Central South UniversityChangshaHunan ProvinceChina Hunan Key Laboratory of Ophthalmology ChangshaHunan ProvinceChina

Glutamate excitotoxicity has been shown to play an important role in glaucoma, and glutamate can induce ferroptosis. The p38 mitogenactivated protein kinase(MAPK) pathway inhibitor SB202190 has a potential ability to suppress ferroptosis, and its downstream targets, such as p53, have been shown to be associated with ferroptosis. However, whether ferroptosis also occurs in retinal ganglion cells in response to glutamate excitotoxicity and whether inhibition of ferroptosis reduces the loss of retinal ganglion cells induced by glutamate excitotoxicity remain unclear. This study investigated ferroptosis in a glutamate-induced glaucoma rat model and explored the effects and molecular mechanisms of SB202190 on retinal ganglion cells. A glutamate-induced excitotoxicity model in R28 cells and an N-methyl-D-aspartate-induced glaucoma model in rats were used. In vitro experiments showed that glutamate induced the accumulation of iron and lipid peroxide and morphological changes of mitochondria in R28 cells, and SB202190 inhibited these changes. Glutamate induced the levels of p-p38 MAPK/p38 MAPK and SAT1 and decreased the expression levels of ferritin light chain, SLC7A11, and GPX4. SB202190 inhibited the expression of iron death-related proteins induced by glutamate. In vivo experiments showed that SB202190 attenuated N-methyl-D-aspartate-induced damage to rat retinal ganglion cells and improved visual function. These results suggest that SB202190 can inhibit ferroptosis and protect retinal ganglion cells by regulating ferritin light chain, SAT1, and SLC7A11/Gpx4 pathways and may represent a potential retina protectant.

关键词： ferroptosis glaucoma glutamate excitotoxicity p38 MAPK retinal ganglion cell SB202190

Starlet:Network defense resource allocation with multi-armed bandits for cloud-edge crowd sensing in IoT

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Digital Communications and Networks 2024年第3期10卷 586-596页

作者： Hui xia Ning Huang Xuecai Feng Rui Zhang chao Liu College of Computer Science and Technology Ocean University of ChinaQingdao266100China

The cloud platform has limited defense resources to fully protect the edge servers used to process crowd sensing data in Internet of *** guarantee the network's overall security,we present a network defense resource allocation with multi-armed bandits to maximize the network's overall ***,we propose the method for dynamic setting of node defense resource thresholds to obtain the defender(attacker)benefit function of edge servers(nodes)and ***,we design a defense resource sharing mechanism for neighboring nodes to obtain the defense capability of ***,we use the decomposability and Lipschitz conti-nuity of the defender's total expected utility to reduce the difference between the utility's discrete and continuous arms and analyze the difference ***,experimental results show that the method maximizes the defender's total expected utility and reduces the difference between the discrete and continuous arms of the utility.

关键词： Internet of things Defense resource sharing Multi-armed bandits Defense resource allocation