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Agricultural Sciences in China 2010年第6期9卷 799-805页

作者： wang Xiao-li wang yu-qian TAO yue-yue FENG Ke College of Environmental Science and Engineering Yangzhou University Yangzhou 225009 P.R. China

Nitrate uptake characteristics and ammonium effects on nitrate uptake were compared between upland rice （Brazilian upland rice） and paddy rice （Wuyujing 3 and Yangdao 6） through the glass microelectrode technique and the concentration gradient method of uptake *** indicated that nitrate uptake by rice seedlings and ammonium effects were depending on membrane potential of root *** upland rice and paddy rice presented obviously different *** all cultivars,the nitrate treatments induced rapid depolarization and then slow repolarization of membrane potential in root epidermal cells,and even hyperpolarization was observed when nitrate concentration was *** membrane potential of epidermal cells in Brazilian upland rice roots was larger and its response to NO3- was bigger than those of two paddy rice *** of membrane potential was amplified when ammonium was simultaneously added with nitrate into the measure medium,but repolarization was reduced,even *** upland rice seedlings had high Vmax of nitrate uptake and low Km,furthermore,Vmax and Km were little affected by ammonium,but Vmax of Wuyujing 3 was reduced ***,inhibition of NH4＋ differed obviously between upland rice and paddy rice.

关键词： nitrate uptake membrane potential ammonium upland rice paddy rice

Membrane-Potential Alteration During K+ Uptake of Different Salt-Tolerant Wheat Varieties

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Agricultural Sciences in China 2010年第7期9卷 974-979页

作者： wang yu-qian wang Xiao-li SHAN yu-hua SHENG Hai-jun FENG Ke Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou 225009 P.R. China School of Life Sciences Guizhou Normal University Guiyang 550001 P.R.China

K＋ is the most abundant cation in plant cells and plays an important role in many ways.K＋ uptake of plant has respect to its salt resistant *** are two categories of channel transportation for plants to uptake K＋,one is through K＋ channels and the other is through nonselective cation channels（NSCCs）.The transmembrane localization of K＋ may change membrane potential（MP）.In this paper,three wheat varieties with different salt tolerance were selected and the MP was measured by microelectrode during K＋ *** results showed that the effects of K＋ uptake on MP through K＋ channels or NSCCs were distinct.K＋ influx through K＋ channels led to MP hyperpolarization,while K＋ influx through NSCCs resulted in *** MP alteration of wheat varieties with different salt tolerance was mainly due to NSCCs-mediated K＋ *** with the salt-tolerant wheat,the MP hyperpolarization during K＋ uptake of saltsensitive wheat was much more evident,probably because of the cation outflux through NSCCs during this process.

关键词： wheat K^+ uptake nonselective cation channels (NSCCs) membrane-potential

A case report of neck, chest and upper limb cutaneous metastasis from synchronous colorectal cancer

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Chinese Medical Journal 2009年第14期122卷 1716-1717页

作者： wang Jian SHI yu-qian WU Zhi-yong Division of General Surgery Ren Ji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200001 China

Cutaneous metastasis from colorectal carcinoma is uncommon, occurring in less than 4% of the whole patients. The most frequently involved are incision scar or abdominal skin, while cutaneous metastasis to neck and chest is very rare. We hereby report a case of synchronous, postoperative cutaneous metastasis from colorectal carcinoma to neck, upper limb and chest skin, which were confirmed by biopsy.

关键词： colorectal cancer cutaneous metastasis

In vivo Confocal Microscopy Evaluation of Meibomian Gland Dysfunction in Dry Eye Patients with Different Symptoms

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Chinese Medical Journal 2016年第21期129卷 2617-2622页

作者： Hui Zhao Jing-Yao Chen yu-qian wang Zhi-Rong Lin Shen wang Eye Institute of Xiamen University Xiamen Fujian 361005 China Department of Ophthalmology & Visual Science Medical College of Xiamen University Xiamen Fujian 361005 China Fujian Provincial Key Laboratory of Ophthalmology and Visual Science Xiamen Fujian 361005 China Department of Corneal & Ocular Surface Diseases Xiamen University Affiliated Xiamen Eye Center Xiamen Fujian 361005 China

Background：Dry eye patients suffer from all kinds of ***,the clinical signs evaluation does not disclose any obvious difference in routine examination;in vivo confocal microscopy （IVCM） is a powerful tool for ocular surface *** study aimed to clarify meibomian gland （MG） alterations in dry eye patients with different symptoms and to compare the findings using ***：A total of sixty patients were recruited,all subjected to Ocular Surface Disease Index （OSDI） and Salisbury Eye Evaluation Questionnaire （SEEQ）,and questionnaires for the assessment of dry eye symptoms before clinical sign examinations were given to the ***,IVCM was applied to observe MG＇s *** analysis was performed using the t-test,Mann-Whitney U-test and Spearman correlation *** differences were statistically significant when P 〈 ***：In the severe symptom group,OSDI and SEEQ scores were significantly higher （P 〈 0.05） compared with the mild symptoms *** other clinical sign examinations had no statistical difference in the two groups （P 〉 0.05）.However,all the IVCM-observed data showed that patients with severe symptoms had more significant fibrosis in MG （acinar unit area 691.87 ± 182.01 μm^2 for the severe,992.17 ± 170.84 μm^2 for the mild;P 〈 0.05） and severer decrease in the size of MG acinar units than those observed in patients with mild symptoms （MG acinar unit density [MGAUD] 70.08 ± 18.78 glands/mm2,MG acinar unit longest diameter [MGALD] 51.50 ± 15.51 μm,MG acinar unit shortest diameter [MGASD] 20.30 ± 11.85 μm for the severe,MGAUD 89.53 ± 39.88 glands/mm2,MGALD 81.57 ± 21.14 μm,MGASD 42.37 ± 14.55 μm for the mild;P 〈 0.05）.Dry eye symptoms were negatively correlated with MG confocal microscopic parameters and positively correlated with conjunctival inflammatory cells and Langerhans cells （P 〈 0.05）.Conclusions：IVCM application provides a strong support to differentiate dry eye patients with different symptoms

关键词： Confocal Microscopy Dry Eye Meibomian Gland

Studies on Blends of Nylon 6 and a Thermotropic Liquid Crystalline Polyarylester(Ⅱ)——Morphology and Rheology

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Chemical Research in Chinese Universities 1992年第2期8卷 121-125页

作者： wang Xia-yu and XIONG qian-zheng (Department of Chemistry, Xiangtan University, Xiangtan, 411005)

Blends of nylon 6 (PA6) and a semirigid thermotropic liquid crystalline polyarylester (THH) were prepared by coprecipitation. The changing of the morphology of the blends with their compositions was observed by means of a polarizing microscope and scanning electron microscope. The THH phases in the blends changed gradually from spherical droplets to fibrils with the increase of THH content. The flow behavior of the blends are quite different from that of the parent polymers and a very dramatic reduction in the melt viscosity of blends containing 5% THH was observed. The formation possibility of in-situ reinforcement composites of PA6 blends with a semirigid LCP is discussed in the present paper.

关键词： Blend Thermotropic Morphology Rheology

Establishment of a Tumor-bearing Mouse Model Stably Expressing Human Tumor Antigens Survivin and MUC1 VNTRs

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Chemical Research in Chinese Universities 2012年第2期28卷 259-263页

作者： ZHANG Li-xing DU Jian-shi wang yu-qian LIU Chen-lu XIA Qiu ZHANG Xi-zhen CONG Xian-ling ZHANG Hai-hong China-Japan Union Hospital of Jilin University Changchun 130033P.R.China National Engineering Lab of AIDS Vaccine College of Life ScienceJilin UniversityChangchun 130012P.R.China

The eukaryotic vectors VR1012 expressing survivin or 33 tandem repeats of human mucin 1（MUC1）（VNTRs）,namely,VR1012-S and VR1012-VNTR（VNTR=variable number of tandem repeat）,were constructed by cloning survivin and VNTR genes into VR1012,*** eukaryotic vector pEGFP expressing survivin and MUC1 VNTRs fusion gene pEGFP-MS was also *** melanoma cell line（B16） stably expressing survivin and MUC1 VNTRs（MS ＋ B16） was established by Lipofectamine-mediated transfection of pEGFP-MS into B16 *** expression in MS ＋ B16 cells was observed using a fluorescent microscope and survivin and MUC1 VNTRs（MS） expression was confirmed by means of Western blot analysis.A syngenic graft tumor model was generated by subcutaneous injection of MS ＋ B16 cells into C57/BL6 mice and tumor size increased rapidly with time in a cell number dependent *** the third immunization,mice were challenged subcutaneously with 5×l0 5 MS ＋ B16 *** with that of the negative control immunized with phosphate-buffered saline（PBS）,a significant reduction of tumor growth was observed in groups immunized with survivin plasmid DNA and MUC1 VNTRs plasmid ***,the suppression of subcutaneous tumor was *** model is useful for the development of tumor vaccines targeting survivin and MUCI VNTRs.

关键词： Survivin MUC1 variable-number tandem repeat(MUC1 VNTR) Tumor antigen Tumor model Tumor vaccine

The Carriage of Escherichia coli Resistant to Antibiotics in Healthy Populations in Shanghai

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Biomedical and Environmental Sciences 1998年第4期11卷 314-320页

作者： ZHANG XIAO-LIN wang FU ZHU DE-MEI WU SHI WU PEI-CHENG CHEN YOU-DI wang yu-qian AND ZHOU LE(Institute of Antibiotics, Huashan Hopital, Shanghai Medical University, Shanghai 200040, China) Institute of Antibiotics Huashan Hospital Shanghai Medical University China.

Healthy populations represent the largest reservoir of bacteria resistant to *** investigated the resistance of Escherichta coli to 12 antibiotics in fecal samples from untreated healthy populations in Shanghai, China by using Kirby-Bauer (K-B) method. The results showed that: (i) All subjects carried resistant strains of Escherichta coli. (ii) The carriage rates of Escherichta coli resistant to various antibiotics were different, less than 10 % to amikacin and 30 % to 100% to others. (iii) In the elder children group aged 10-11 years, the percentages of stralns resistant to gentamicin, streptomycin, chloramphenicol, tetracycline, trimethoprim, and sulfamethoxazole were significantly lower than those in the younger group aged 5-6 years. In the adult group, the percentages of strains resistant to ampicillin, piperacillin, arnikacin, streptomycin, chloramphenicol, tetracycline, trimethoprim, and sulfamethoxarole were significantly lower than those in the elder children group. (iv) The number of strains resistant to five or more antibiotics accounted for 31. 8 % in the younger children group, 23. 7 % in the elder children group,and 12. 1 % in the adult group. These findings suggest that all healthy people in Shanghal carry resistant strains of Escherichta coli in the intestine. The younger the populations, the higher the level of resistance of fecal Escherichta coli to antibiotics. Improvement of health behaviors and environmental sanitation and rational use of antibiotics could remarkedly decrease the resistant level of bacteria

关键词： coli The Carriage of Escherichia coli Resistant to Antibiotics in Healthy Populations in Shanghai

长江经济带新型城镇化与水环境耦合协调及其驱动因素研究

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长江流域资源与环境 2024年第3期33卷 572-583页

作者：张宁王昱倩杭州电子科技大学管理学院浙江杭州310018 杭州电子科技大学法学院浙江杭州310018

新型城镇化进程在促进社会经济快速发展的同时也导致水环境问题日益严峻。以长江经济带11个省份(直辖市)为研究对象,采用熵值法与耦合协调度模型测算新型城镇化与水环境的耦合协调度,并借助空间自相关模型与灰色关联度模型探讨二者耦合... 详细信息

新型城镇化进程在促进社会经济快速发展的同时也导致水环境问题日益严峻。以长江经济带11个省份(直辖市)为研究对象,采用熵值法与耦合协调度模型测算新型城镇化与水环境的耦合协调度,并借助空间自相关模型与灰色关联度模型探讨二者耦合协调度的时空演化及驱动因素。结果显示:(1) 2009~2020年,长江经济带水环境综合水平指数保持平稳,新型城镇化与二者间的耦合协调度整体发展趋势尚好,从空间演变来看,耦合协调度呈现“下游>中游>上游”的分布格局,且区域间差异有所缓和,但整体增长速率较为缓慢。(2)二者的耦合协调度具有显著的正向空间自相关性,且局部的空间自相关表现为聚集趋同特征,空间关联性较强。(3)影响二者耦合协调度的因素按作用强度从大到小依次为产业结构、人口聚集、技术创新、经济驱动和政府能力,且各因素对耦合协调度的作用程度具有明显的地区差异。因此,需充分发挥长江上中下游区域资源优势,推动新型城镇化与水环境协调发展。

关键词：新型城镇化水环境耦合协调度灰色关联度

Relationship between bed shear stress and suspended sediment concentration:annular flume experiments

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International Journal of Sediment Research 2011年第4期26卷 513-523页

作者： yunwei wang,qian yu,and Shu GAO 1,2 Dr.,3 Prof.,Ministry of Education Key Laboratory for Coast and Island Development,Nanjing University, Hankou Road 22,210093 Nanjing,China, [a] Dr. Ministry of Education Key Laboratory for Coast and Island Development Nanjing University Hankou Road 22 210093 Nanjing China [b] Dr. Ministry of Education Key Laboratory for Coast and Island Development Nanjing University Hankou Road 22 210093 Nanjing China [c] Prof. Ministry of Education Key Laboratory for Coast and Island Development Nanjing University Hankou Road 22 210093 Nanjing China

In this study,annular flume experiments were carried out,using the sediment samples collected from the lower part of the inter-tidal zone at Xiaoyangkou,Jiangsu coast,*** Ariathurai-Partheniades equation was used to determine the bed shear stress,by evaluating variations in the suspended sediment concentration within the water *** derived relation between the bed shear stress and suspended sediment concentration shows that,at various stages of seabed erosion, suspended sediment concentration increases rapidly when the flow velocity is increased,but the pattern of change in the bed shear stress does not follow *** low concentrations,bed shear stress initially increases markedly with increasing flow ***,when the concentration reaches an apparently critical level around 0.55 kg m"3,the rate of change in the bed shear stress abruptly slows down,or becomes almost constant,in response to further increases in the flow *** of experiments indicate that,from a critical level onward,suspended sediment concentration has a strong influence on the bed shear stress.

关键词： Bed shear stress Suspended sediment concentration Annular flume

Effects of Methylated Soybean Oil Adjuvant on Fomesafen Efficacy to Weeds

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Journal of Northeast Agricultural University(English Edition) 2014年第3期21卷 17-22页

作者： Han yu-jun Fu Jiu-cai wang qian-yu Tao Bo Mao Zi-jun Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme Northeast Forestry University Postdoctoral Programme College of Agriculture Northeast Agricultural University Jiamusi Branch Heilongjiang Academy of Agricultural Sciences

Tank-mix adjuvant has the potential to improve the weed control efficacy of post-emergence herbicides. In order to study the synergistic effect of adjuvant, the effects of different rates of fomesafen alone or applied methylated soybean oil adjuvant（MSO） were sprayed on redroot pigweed, abutilon and black nightshade under greenhouse condition. The results showed that fomesafen had different performance on the three weeds, and MSO adjuvant could effectively increase the control. The nightshade control was lower than other two weeds with all the fomesafen doses from 131.25 to 506.25 ga.i. · hm-2 with or without adjuvant. The control of abutilon was between the black nightshade and the redroot pigweed, and had better control at 375 ga.i. · hm-2 with adjuvant or 506.25 ga.i. · hm-2 alone or with adjuvant respectively. The results indicated that mixing adjuvant with fomesafen improved the control on weeds, especially at the low rate. Black nightshade was more difficult to control. The redroot pigweed had the most susceptibility to fomesafen alone or with adjuvant.

关键词： fomesafen methylated soybean oil adjuvant(MSO) weed control synergistic effect