Establishment of a Tumor-bearing Mouse Model Stably Expressing Human Tumor Antigens Survivin and MUC1 VNTRs

作     者：ZHANG Li-xing DU Jian-shi WANG Yu-qian LIU Chen-lu XIA Qiu ZHANG Xi-zhen CONG Xian-ling ZHANG Hai-hong 

作者机构：China-Japan Union Hospital of Jilin UniversityChangchun 130033P.R.China National Engineering Lab of AIDS VaccineCollege of Life ScienceJilin UniversityChangchun 130012P.R.China 

出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)

年 卷 期：2012年第28卷第2期

页      面：259-263页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Natural Science Foundation of China(No.30872396) the Scientific Research Foundation of Jilin Province,China(Nos.20080709,200905169) the Jilin University Basic Research Project,China(No.200903255) 

主　　题：Survivin MUC1 variable-number tandem repeat(MUC1 VNTR) Tumor antigen Tumor model Tumor vaccine 

摘      要：The eukaryotic vectors VR1012 expressing survivin or 33 tandem repeats of human mucin 1（MUC1）（VNTRs）,namely,VR1012-S and VR1012-VNTR（VNTR=variable number of tandem repeat）,were constructed by cloning survivin and VNTR genes into VR1012,*** eukaryotic vector pEGFP expressing survivin and MUC1 VNTRs fusion gene pEGFP-MS was also *** melanoma cell line（B16） stably expressing survivin and MUC1 VNTRs（MS ＋ B16） was established by Lipofectamine-mediated transfection of pEGFP-MS into B16 *** expression in MS ＋ B16 cells was observed using a fluorescent microscope and survivin and MUC1 VNTRs（MS） expression was confirmed by means of Western blot analysis.A syngenic graft tumor model was generated by subcutaneous injection of MS ＋ B16 cells into C57/BL6 mice and tumor size increased rapidly with time in a cell number dependent *** the third immunization,mice were challenged subcutaneously with 5×l0 5 MS ＋ B16 *** with that of the negative control immunized with phosphate-buffered saline（PBS）,a significant reduction of tumor growth was observed in groups immunized with survivin plasmid DNA and MUC1 VNTRs plasmid ***,the suppression of subcutaneous tumor was *** model is useful for the development of tumor vaccines targeting survivin and MUCI VNTRs.