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Science China Chemistry 2011年第8期54卷 1273-1276页

作者： GE ZhiLei PEI Hao wang lihua SONG ShiPing FAN ChunHai Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 China

An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM.

关键词： DNA nanostructure surface density electrochemical

A new dynamic method for measuring hydrogen partial pressure in molten aluminum alloy

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China Foundry 2011年第1期8卷 1-4页

作者： Sun Qian Li Dayong wang lihua Zhang Jianlei Harbin University of Science ＆ Technology Harbin 150080 China

Hydrogen partial pressure is an important parameter to calculate hydrogen concentration levels in molten aluminum alloy. A new dynamic method for measuring hydrogen partial pressure in molten aluminum alloy is studied. Dynamic and rapid measurement is realized through changing the volume of the vacuum chamber and calculating the pressure difference ΔP between the theoretical and measured pressures in the vacuum chamber. Positive ΔP indicates hydrogen transmits from melt to vacuum chamber and negative ΔP means the reverse. When ΔP is equal to zero, hydrogen transmitted from both sides reached a state of dynamical equilibrium and the pressure in the vacuum chamber is equal to the hydrogen partial pressure in the molten aluminum alloy. Compared with other existing measuring methods, the new method can significantly shorten the testing time and reduce measuring cost.

关键词： molten aluminum alloy hydrogen partial pressure dynamic measurement rapid measurement

Antiferromagnetic element Mn modified PtCo truncated octahedral nanoparticles with enhanced activity and durability for direct methanol fuel cells

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Nano Research 2019年第10期12卷 2520-2527页

作者： Qiqi Zhang Jialong Liu Tianyu Xia Jie Qi Haochang Lyu Baoyuan Luo Rongming wang Yizhong Guo lihua wang and Shouguo wang Beijing Advanced Innovation Center for Materials Genome Engineering School of Materials Science and EngineeringUniversity of Science and Technology BeijingBeijing 100083China Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China Key Laboratory of Material Physics of Ministry of Education School of Physics and EngineeringZhengzhou UniversityZhengzhou 450052China Institute far multidisciplinary Innovation University of Science and Technology BeijingBeijing 100083China Beijng Key Lab cf Microstructure and Property of Advanced Materials Beijing University of TechnologyBeijing 100124China

Pt-based magnetic nano catalysts are one of the most suitable cand idates for electrocatalytic materials due to their high electrochemistry activity and ***,the inferior durability prevents them from being scaled-up,limiting their commercial ***,an antiferromagnetic element Mn was introduced into PtCo nanostructured alloy to synthesize uniform Mn-PtCo truncated octahedral nanoparticles(TONPs)by one-pot *** results show that Mn can tune the blocking temperature of Mn-PtCo TONPs due to its an tiferromag *** low temperatures,Mn-PtCo TONPs are ferromag netic,and the coercivity in creases gradually with in creasi ng Mn *** room temperature,the Mn-PtCo TONPs display superparamag netic behavior,which is greatly helpful for in dustrial *** doping can not only modify the electronic structure of PtCo TONPs but also enhanee electrocatalytic performance for methanol oxidation *** maximum specific activity of Mn-PtCo-3 reaches 8.1 A`m^-2,3.6 times of commercial Pt/C(2.2 A·m^-2)and 1.4 times of PtCo TONPs(5.6 A`m^-2),*** mass activity decreases by only 30%after 2,000 cycles,while it is 45%and 99%(nearly inactive)for PtCo TONPs and commercial Pt/C catalysts,respectively.

关键词： Mn-PtCo trun cated octahedral nano particles an tiferromag netic eleme nt magnetic properties electrocatalytic performa nee

Trace nitrogen-doped hierarchical porous biochar nanospheres:Waste corn roots derived superior adsorbents for high concentration single and mixed organic dyes removal

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Nano Research 2023年第2期16卷 1846-1858页

作者： lihua wang Hongwei Li Mingyue Li Liyun Zhang Heng Zhang Zi-Yu Liu Wancheng Zhu Department of Chemical Engineering Qufu Normal UniversityQufu 273165China Institute of Engineering Thermophysics Chinese Academy of SciencesBeijing 100190China

Seeking high performance adsorbents for highly efficient treatment of wastewater containing organic dyes has become increasingly imperative ***,with a specific surface area(SSA)of 2,745.4 m^(2)·g^(−1),trace N-doped porous biochar nanospheres(NPBs)are derived for the first time from affluent waste corn roots,via a hydrothermal conversion followed by a mild calcined activation by K2CO3(KC)in the presence of low virulent *** acts as N source and synergistic activator for significant promotion in SSA,pore volume,and surface *** obtained NPBs(CHC-0.5N-4KC-900)are confirmed as superior adsorbents for removal of organic dyes rhodamine B(RhB,qm=1,630.7 mg·g^(−1))and Congo red(CR,qm=1,766.2 mg·g^(−1))as well as their mixtures,within not only a low(50,esp.250–1500 mg·L^(−1))concentration *** values for qm are far beyond commercially activated carbon(AC)as well as most reported biomass derived carbons,undoubtedly revealing the NPBs as great promising candidate adsorbents for disposal of real industrial *** addition,the adsorption of RhB is fitted by Langmuir,Freundlich,Temkin,and Dubinin–Radushkevich isotherm *** kinetic analysis indicates that the adsorption before equilibrium conforms to the pseudo-second-order model,and the hydrogen bonding,electrostatic attraction,and esp.π–πinteraction have contributed to the superior adsorption performance of the NPBs.

关键词： biomass corn roots N-doped carbons biochar organic dyes adsorption

Phenotypic Characterization of Porcine IFNc-Producing Lymphocytes in Porcine Reproductive and Respiratory Syndrome Virus Vaccinated and Challenged Pigs

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Virologica Sinica 2018年第6期33卷 524-530页

作者： Xiangdong Li Zengyang Pei Yilin Bai lihua wang Jishu Shi Kegong Tian National Research Center for Veterinary Medicine Zhengzhou 450002China Department of Anatomy and Physiology College of Veterinary MedicineKansas State UniversityLuoyang 471003China Department of Veterinary Medicine College of Animal ScienceZhejiang UniversityManhattan 66506USA College of Veterinary Medicine Northwest A&F UniversityYangling 712100China College of Animal Science and Veterinary Medicine Henan Agricultural UniversityHangzhou 310058China OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory China Animal Disease Control CenterBeijing 100125China

Porcine reproductive and respiratory syndrome(PRRS) continues to be one of the most important swine diseases worldwide. Interferon-γ(IFNγ)-mediated type Ⅰ cell-mediated immune response plays an important role in protection from,and clearance of, PRRS virus(PRRSV). Several lymphocyte subsets including T-helper, CTLs, Th/memory cells, and cd T lymphocytes were previously reported to produce IFNc during PRRSV infection. However, the proportion and phenotypic characterization of these IFNγ-secreting lymphocytes have not been explored. In this study, IFNc producted by different lymphocyte subsets was assessed by multi-color flow cytometry after vaccination with PRRSV modified live vaccine(PRRSV-MLV) and challenge with homogeneous or heterogeneous PRRSV. The results showed that T-helper cells were the major IFNγ-secreting cell population after PRRSV-MLV vaccination and PRRSV challenge. Additionally, the proportion of IFNγ producing Th/memory cells and γδ T cells increased after PRRSV challenge. This difference was accounted for an enhanced ability to produce IFNγ in Th/memory cells and an enlarged quantity of γδ T cells. The results presented here could contribute to our understanding of the roles of IFNγ in protective immunity against PRRSV infection and may be useful for assessment of cell-mediated immunity in vaccine tests.

关键词： Porcine reproductive and respiratory syndrome virus(PRRSV) Lymphocyte Interferon-γ(IFNγ)

Recent Advances and Future Directions of Diagnostic and Prognostic Prediction Models in Ovarian Cancer

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Journal of Shanghai Jiaotong university(Science) 2021年第1期26卷 10-16页

作者： ZENG Judan CAO Wenjiao wang lihua Department of Obstetrics and Gynecology the International Peace Maternity&Child Health Hospital of China Welfare InstituteShanghai Jiao Tong University School of MedicineShanghai 200030China

Ovarian cancer has one of the highest mortality rates among gynecological *** disease has a low early detection rate,a high postoperative recurrence rate,and a 5-year survival rate of only 40%.Hence,there is an urgent need to improve the early diagnosis and prognosis of ovarian *** models can effectively estimate the risk of disease occurrence,as well as its ***,many studies have established multiple ovarian cancer prediction models based on different regions and *** models can improve the detection rate and optimize the prognosis management to a certain ***,the construction principle of the ovarian cancer risk prediction model and its validation are summarized;furthermore,comprehensive reviews and comparisons of the different types of these models are ***,our review may be of great significance for the whole course of ovarian cancer management.

关键词： ovarian cancer diagnostic prediction model prognostic prediction model

Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope

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Science China Chemistry 2016年第11期59卷 1519-1524页

作者： Shaopeng wang Suhui Deng Xiaoqing Cai Shangguo Hou Jiajun Li Zhaoshuai Gao Jiang Li lihua wang Chunhai Fan Division of Physical Biology&Bioimaging Center Shanghai Synchrotron Radiation FacilityCAS Key Laboratory of Interfacial Physics and TechnologyShanghai Institute of Applied PhysicsChinese Academy of SciencesShanghai 201800China University of Chinese Academy of Sciences Beijing 100049China School of Information Engineering Nanchang UniversityNanchang 330031China

The significant role of telomeres in cells has attracted much attention since they were *** imaging is an effective method to study subcellular structures like ***,the diffraction limit of traditional optical microscope hampers further investigation on *** progress on superresolution fluorescence microscopy has broken this *** this work,we used stimulated emission depletion(STED) microscope to observe fluorescence-labeled telomeres in interphase cell *** results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal *** adjacent telomeres were clearly separated via STED imaging,which could hardly be discriminated by confocal microscopy due to the diffraction *** conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres.

关键词： telomere superresolution imaging stimulated emission depletion(STED) microscopy fluorescence in situ hybridization(FISH)

Umbilical cord-derived mesenchymal stem cells regulate thymic epithelial cell development and function in Foxn 1^-/- mice

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Cellular & Molecular Immunology 2014年第3期11卷 275-284页

作者： Guangyang Liu lihua wang Tianxiang Pang Delin Zhu Yi Xu Hanyu wang Xiuli Cong Yongjun Liu Key Laboratory of Systems Bioengineering School of Chemical Engineering and Technology Tianjin University Tianjin China AIliancells Institute of Stem Cells and Translational Regenerative Medicine Tianjin China The State Key Laboratory of Experimental Hematology Institute of Hematology and Hospital of Blood Diseases Graduate School of Chinese Academy of Medical Sciences and Peking Union of Medical College Tianjin China University of Florida Department of Medicine Gainesville FL USA GL and LW are cofirst authors and contributed equally to this study

Thymic microenvironments are essential for the maturation of thymocytes, which can be anatomically compartmentalized into cortical and medullar regions. The absence of the gene encoding the transcription factor forkhead box nl （Foxnl） causes epithelial differentiation to stall in the precursor stage, resulting in the formation of an abnormal thymus. In this study, we used human umbilical cord-derived mesenchymal stem cells （UC-MSCs） to treat Foxn1^-/- mice, and then analyzed the maturation and distribution of thymic epithelial cells in the Foxn1^-/- thymic rudiment and the thymopoiesis of this newly developed rudiment. Our data showed a well-organized cortex-medulla architecture and an obvious improvement in the maturation of thymic epithelial ceils along with the appearance of UEA-1^＋MHCIIhi thymic epithelial cells in the rudiment. We further demonstrated improved thymopoiesis and the enhanced export of mature T cells with increased numbers of regulatory T cells into the peripheral blood. Furthermore, we observed that MSCs can engraft into thymic tissue and express many cytokines or proteins, particularly keratinocyte growth factor （KGF） and CD248, which are essential for thymic development. Collectively, our data identified a new mechanism for MSCs, which may provide a proper microenvironment for the reconstitution and functional maturation of the thymus in Foxn1^-/- mice. Additionally, we elicited additional insights into the therapeutic efficacy of MSCs in several autoimmune diseases.

关键词： keratinocyte growth factor mesenchymal stem cells thymus development regulatory T cells

Structurally reconfigurable designer RNA structures for nanomachines

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Biophysics Reports 2021年第1期7卷 21-34页

作者： Kai Jiao Yaya Hao Fei wang lihua wang Chunhai Fan Jiang Li Division of Physical Biology CAS Key Laboratory of Interfacial Physics and Technology Shanghai Institute of Applied Physics Chinese Academy of Sciences The Interdisciplinary Research Center Shanghai Synchrotron Radiation Facility Zhangjiang Laboratory Shang-hai Advanced Research Institute Chinese Academy of Sciences University of Chinese Academy of Sciences School of Chemistry and Chemical Engineering Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine Shanghai Jiao Tong University

Structurally reconfigurable RNA structures enable dynamic transitions of the functional states in response to diverse molecular stimuli, which are fundamental in genetic and epigenetic regulations. Inspired by nature, rationally designed RNA structures with responsively reconfigurable motifs have been developed to serve as switchable components for building engineered nanomachines, which hold promise in synthetic biological applications. In this review, we summarize recent progress in the design, synthesis, and integration of engineered reconfigurable RNA structures for nanomachines. We highlight recent examples of their targeted applications such as biocomputing and smart *** also discuss their advantages, challenges as well as possible solutions. We further provide an outlook of their potential in future synthetic biology.

关键词： RNA nanomachine Structure Reconfiguration Biocomputing Theranostics

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Chinese Medical Sciences Journal 2017年第1期32卷 65-68页

作者： Xing Chen lihua wang Lixian Zhang Caihong Zhao Department of Urology China-Japan Friendship Hospital Department of Gastroenterology Harrison International Peace Hospital

IGG4-RELATED autoimmune pancreatitis (AIP) is themost common type of IgG4-related diseases. Theobservation of a diffused and localized enlargementof the pancreas and strictures in the main pancreaticduct on radiological imaging is essential fordiagnosing AIP. The clinical features of AIP which presentas a localized pancreatic mass greatly mimic pancreaticcancer. Cases with AIP mimicking pancreatic cancer arereported numerously. However, cases of IgG4-related AIPmanifesting acute pancreatitis symptoms and extensiveperipancreatic effusion are rarely observed. Meanwhile,acute pancreatitis is a common disease. If misdiagnosed asacute pancreatitis, IgG4-related AIP may progressseriously. Here, we report a rare case of IgG4-related AIPsuspected of acute pancreatitis because of epigastric painand extensive peripancreatic effusion on computed tomography(CT) images.

关键词： autoimmune pancreatitis IgG4-related diseases computed tomography