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研究生细胞生物学大实验课程改革探索

实验室研究与探索 2016年第10期35卷 166-169页

作者：卢存福荆艳萍万迎朗北京林业大学生物科学与技术学院北京100083

细胞生物学是现代生命科学中的重要基础前沿学科,新技术新方法的迅速发展是学科发展的动力源泉。因此,细胞生物学实验课程体系的改革和建设成为培养研究生综合能力的重要环节。以研究生细胞生物学大实验课程体系的改革为依托,通过采用... 详细信息

细胞生物学是现代生命科学中的重要基础前沿学科,新技术新方法的迅速发展是学科发展的动力源泉。因此,细胞生物学实验课程体系的改革和建设成为培养研究生综合能力的重要环节。以研究生细胞生物学大实验课程体系的改革为依托,通过采用模块式教学内容重构研究生实验教学体系,改进教学方法和手段。通过本课程的学习,使研究生能够在入学后尽快掌握科学研究的方法和科学研究的新思路,综合科研素质获得提高,从而为学位论文的研究工作奠定良好的基础。

关键词：细胞生物学综合大实验教学改革

Infiltration by monocytes of the central nervous system and its role in multiple sclerosis: reflections on therapeutic strategies

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Neural Regeneration Research 2025年第3期20卷 779-793页

作者： Guangyong Zhang Qing Yao Chubing Long Pengcheng Yi Jiali Song Luojia Wu Wei wan Xiuqin Rao Yue Lin Gen Wei Jun ying Fuzhou Hua Department of Anesthesiology the Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi ProvinceChina Key Laboratory of Anesthesiology of Jiangxi Province NanchangJiangxi ProvinceChina

Mononuclear macrophage infiltration in the central nervous system is a prominent feature of neuroinflammation. Recent studies on the pathogenesis and progression of multiple sclerosis have highlighted the multiple roles of mononuclear macrophages in the neuroinflammatory process. Monocytes play a significant role in neuroinflammation, and managing neuroinflammation by manipulating peripheral monocytes stands out as an effective strategy for the treatment of multiple sclerosis, leading to improved patient outcomes. This review outlines the steps involved in the entry of myeloid monocytes into the central nervous system that are targets for effective intervention: the activation of bone marrow hematopoiesis, migration of monocytes in the blood, and penetration of the blood–brain barrier by monocytes. Finally, we summarize the different monocyte subpopulations and their effects on the central nervous system based on phenotypic differences. As activated microglia resemble monocyte-derived macrophages, it is important to accurately identify the role of monocyte-derived macrophages in disease. Depending on the roles played by monocyte-derived macrophages at different stages of the disease, several of these processes can be interrupted to limit neuroinflammation and improve patient prognosis. Here, we discuss possible strategies to target monocytes in neurological diseases, focusing on three key aspects of monocyte infiltration into the central nervous system, to provide new ideas for the treatment of neurodegenerative diseases.

关键词： blood–brain barrier macrophages monocytes multiple sclerosis neuroinflammation review therapy

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植物光合作用循环电子传递的研究进展

植物生理学报 2017年第2期53卷 145-158页

作者：薛娴许会敏吴鸿洋沈应柏肖建伟万迎朗北京林业大学生物科学与技术学院北京100083 河南科技大学农学院河南洛阳471023

在植物光合作用的光反应中,由叶绿素等色素分子收集的光能通过光系统I(PSI)和光系统II(PSII)驱动了光合电子传递进程。当电子最终被传递到高能化合物NADPH和ATP中,完成光能到化学能的转换并为固碳反应提供能量的过程,被称为线形电子流(L... 详细信息

在植物光合作用的光反应中,由叶绿素等色素分子收集的光能通过光系统I(PSI)和光系统II(PSII)驱动了光合电子传递进程。当电子最终被传递到高能化合物NADPH和ATP中,完成光能到化学能的转换并为固碳反应提供能量的过程,被称为线形电子流(LEF)。而电子仅绕着PSI传递,通过形成跨膜质子梯度而驱动ATP酶合成ATP的过程,被称为循环电子流(CEF)。近年来,CEF的催化机制、生理作用和调控机制等吸引了研究者们的关注,特别是反向遗传学的发展,为更深入理解循环电子传递带来契机。本文综述了CEF的研究历史和最新进展,并从植物逆境适应以及植物进化等角度进一步分析了CEF的形成与生理意义,为今后的相关研究提供参考。

关键词：循环电子流线形电子流光合作用光合磷酸化

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海南省首次发现光合软体动物绒毛海天牛

动物学杂志 2022年第5期57卷 775-781页

作者：章可兰李歆毓余冬万迎朗海南大学热带作物学院海口570228

本文首次报道了光合软体动物Elysia tomentosa ***,1997在我国海南省的分布情况。2021年9月,本课题组在海南省文昌市云龙湾采集到该物种,通过线粒体基因组核糖体16S rRNA以及组蛋白H3基因(H3)位点部分DNA序列比对,确定该物种为Elysia to... 详细信息

本文首次报道了光合软体动物Elysia tomentosa ***,1997在我国海南省的分布情况。2021年9月,本课题组在海南省文昌市云龙湾采集到该物种,通过线粒体基因组核糖体16S rRNA以及组蛋白H3基因(H3)位点部分DNA序列比对,确定该物种为Elysia tomentosa。参考该物种学名并根据其外部形态特征,建议该物种中文学名为“绒毛海天牛”。通过聚合酶链式反应(PCR)鉴定其生活环境中的藻类,确定了该物种共生叶绿体的来源为总状蕨藻(Caulerpa racemose);利用叶绿素荧光诱导曲线,检测了绒毛海天牛的光合荧光特征。这是本课题组在海南省沿海地区发现分布的又一种光合软体动物,进一步丰富了对海南岛沿岸海天牛分布的认知,也为研究光合作用的进化与内共生机理提供新的研究材料。

关键词：海天牛属系统发育树分布新记录种绒毛海天牛

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海南省海口市发现白边侧足海天牛

动物学杂志 2021年第4期56卷 631-635页

作者：李歆毓崔闯安启源万迎朗海南大学热带作物学院海口570228

本文介绍了一种海天牛(Elysia leucolegnote)在海南岛的新分布情况。***属于软体动物门囊舌目(Sacoglossa)海天牛超科(Elysioidea)海天牛属。其以某种丝状绿藻(Boodleopsis sp.)为食,从中摄取叶绿体,并具有在动物细胞中保持叶绿体光合... 详细信息

本文介绍了一种海天牛(Elysia leucolegnote)在海南岛的新分布情况。***属于软体动物门囊舌目(Sacoglossa)海天牛超科(Elysioidea)海天牛属。其以某种丝状绿藻(Boodleopsis sp.)为食,从中摄取叶绿体,并具有在动物细胞中保持叶绿体光合活性的独特能力。这种海天牛类群在进化生物学、光合生物学和生理学研究中受到广泛关注。2020年5月,在海南省海口市的东寨港国家级红树林自然保护区的旗调村发现这种海天牛,通过形态对比和分子标记分析确认为***,因其在国内尚无报道,建议根据学名和外观特征命名为白边侧足海天牛。通过线粒体COⅠ基因的序列比对,构建了我国已知海天牛属物种的进化树。并且在2020年5月到11月间,在白边侧足海天牛生长地跟踪观察并描述其形态特征变化和行为特征。

关键词：光合软体动物海天牛属分布新记录种红树林白边侧足海天牛

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植物中活性氧信号转导及其检测方法研究进展

电子显微学报 2014年第2期33卷 188-196页

作者：赵晓玉薛娴卢存福林金星万迎朗北京林业大学生物科学与技术学院北京100083

活性氧(ROS)作为一种重要信号分子在植物体生命活动的多方面发挥着关键作用。为对活细胞内的ROS分子进行研究分析,人们发展出了多种检测手段。其中,荧光探针作为一种高度灵敏、直观的标记技术,在植物ROS研究中得到广泛应用。近年来,通... 详细信息

活性氧(ROS)作为一种重要信号分子在植物体生命活动的多方面发挥着关键作用。为对活细胞内的ROS分子进行研究分析,人们发展出了多种检测手段。其中,荧光探针作为一种高度灵敏、直观的标记技术,在植物ROS研究中得到广泛应用。近年来,通过蛋白质工程和基因工程技术,研究者们基于绿色荧光蛋白创造出一批新型的特异性荧光探针,为进一步探索植物体内ROS信号途径提供更有力的工具。本文概述了植物细胞中ROS信号转导通路,并总结针对不同ROS组分的多种研究方法,尤其对利用荧光探针标记技术检测胞内ROS的研究进展进行详细介绍。

关键词：活性氧信号传导植物细胞荧光标记显微探测

The Subcellular Localization and Blue-Light- Induced Movement of Phototropin 1-GFP in Etiolated Seedlings of Arabidopsis thalianaw

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Molecular Plant 2008年第1期1卷 103-117页

作者： ying-lang wan William Eisinger David Ehrhardt Ulrich Kubitscheck Frantisek Baluska Winslow Briggs Dept Plant Cell Biology Institute of Cellular and Molecular Botany University of Bonn Kirschallee 1 D-53115 Bonn Germany Dept Biology Santa Clara University Santa Clara CA 95053 Dept Plant Biology Carnegie Institution of Washington Stanford CA 94305 Institute for Physical and Theoretical Chemistry Wegelerstr. 12 D-53115 Bonn Germany

Phototropin 1 （photl） is a photoreceptor for phototropism, chloroplast movement, stomatal opening, leaf expansion, and solar tracking in response to blue light. Following earlier work with PHOTI：：GFP （Sakamoto and Briggs, 2002）, we investigated the pattern of cellular and subcellular localization of photl in 3-4 d old etiolated seedlings of Arabidopsis thalinana. As expressed from native upstream sequences, the PHOTT：GFP fusion protein is expressed strongly in the abaxial tissues of the cotyledons and in the elongating regions of the hypocotyl. It is moderately expressed in the shoot/root transition zone and in cells near the root apex. A fluorescence signal is undetectable in the root epidermis, root cap, and root apical meristem itself. The plasma membranes of mesophyll cells near the cotyledon margin appear labeled uniformly but cross-walls created by recent cell divisions are more strongly labeled. The pattern of labeling of individual cell types varies with cell type and developmental stage. Blue-light treatment causes PHOTI：：GFP, initially relatively evenly distributed at the plasma membrane, to become reorganized into a distinct mosaic with strongly labeled punctate areas and other areas completely devoid of fluorescenco a phenomenon best observed in cortical cells in the hypocotyl elongation region. Concomitant with or following this reorganization, PHOTT：GFP moves into the cytoplasm in all cell types investigated except for guard cells. It disappears from the cytoplasm by an unidentified mechanism after several hours in darkness. Neither its appearance in the cytoplasm nor its eventual disappearance in darkness is prevented by the translation inhibitor cycloheximide, although the latter process is retarded. We hypothesize that blue-light-induced photl relocalization modulates blue-light-activated signal transduction.

关键词： Phototropin 1 (phot1) PHOT1::GFP Arabidopsis thalianaw

DBST:a lightweight block cipher based on dynamic S-box

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Frontiers of Computer Science 2023年第3期17卷 177-185页

作者： Liuyan YAN lang LI ying GUO College of Computer Science and Technology Hengyang Normal UniversityHengyang 421002China Hunan Provincial Key Laboratory of Intelligent Information Processing and Application Hengyang Normal UniversityHengyang 421002China

IoT devices have been widely used with the advent of *** devices contain a large amount of private data during *** is primely important for ensuring their ***,we proposed a lightweight block cipher based on dynamic S-box named *** is introduced for devices with limited hardware resources and high throughput *** is a 128-bit block cipher supporting 64-bit key,which is based on a new generalized Feistel variant *** retains the consistency and significantly boosts the diffusion of the traditional Feistel *** SubColumns of round function is implemented by combining bit-slice technology with *** S-box is dynamically associated with the *** has been demonstrated that DBST has a good avalanche effect,low hardware area,and high *** S-box has been proven to have fewer differential features than RECTANGLE *** security analysis of DBST reveals that it can against impossible differential attack,differential attack,linear attack,and other types of attacks.

关键词： internet of things 5G dynamic S-box bit-slice technology lightweight block cipher

Rutin pretreatment promotes microglial M1 to M2 phenotype polarization

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Neural Regeneration Research 2021年第12期16卷 2499-2504页

作者： Guang-Ping lang Can Li ying-ying Han Key Laboratory of Basic Pharmacology of Ministry of Education and Joint International Research Laboratory of Ethnomedicine of Ministry of Education Zunyi Medical UniversityZunyiGuizhou ProvinceChina Special Key Laboratory of Oral Diseases Research Higher Education Institutions of Guizhou ProvinceZunyi Medical UniversityZunyiGuizhou ProvinceChina

Microglial cells are important resident innate immune components in the central nervous system that are often activated during *** microglia can display one of two phenotypes,M1 or M2,which each play distinct roles in ***,a dietary flavonoid,exhibits protective effects against ***,whether rutin is able to influence the M1/M2 polarization of microglia remains *** this study,in vitro BV-2 cell models of neuroinflammation were established using 100 ng/mL lipopolysaccharide to investigate the effects of 1-hour rutin pretreatment on microglial *** results revealed that rutin pretreatment reduced the expression of the proinflammatory cytokines tumor necrosis factor-α,interleukin-1β,and interleukin-6 and increased the secretion of *** pretreatment also downregulated the expression of the M1 microglial markers CD86 and inducible nitric oxide synthase and upregulated the expression of the M2 microglial markers arginase 1 and *** pretreatment inhibited the expression of Toll-like receptor 4 and myeloid differentiation factor 88 and blocked the phosphorylation of I kappa B kinase and nuclear factor-kappa *** results showed that rutin pretreatment may promote the phenotypic switch of microglia M1 to M2 by inhibiting the Toll-like receptor 4/nuclear factor-kappa B signaling pathway to alleviate lipopolysaccharide-induced neuroinflammation.

关键词： BV-2 cells flavonoid M1 microglia M2 microglia microglia activation microglia polarization neuroinflammation proinflammatory cytokines rutin TLR-4 signaling