检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

大陆与台湾刑事诉讼中回避制度之比较研究

西安建筑科技大学学报(社会科学版) 2000年第2期19卷 48-50页

作者：杨粉米西安建筑科技大学社科系!西安 710055 讲师

回避制度是世界各国刑事诉讼的重要制度之一，本文通过对大陆与台湾刑事诉讼中回避制度的比较研究，以期互相吸收借鉴彼此的优点，改革与完善刑事司法回避制度。

关键词：刑事诉讼回避制度比较

Chinese Prescription kangen-karyu as Potential Anti-Alzheimer’s Disease Therapeutic:Analyses of BACE1 and GSk-3βInhibitory Activities

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Medicine（双语） 2021年第2期10卷 37-42页

作者： Chan hum Park Min Jo kim Hyun Ah Jung Jae Sue Choi Jin Pyeong Jeon Takako Yokozawa Institute of New Frontier Research Team Hallym Clinical and Translational Science InstituteHallym UniversityChuncheon24252Republic of Korea Department of Medicinal Crop Research National Institute of Horticultural and Herbal ScienceRural Development AdministrationEumseong369-873Republic of Korea Department of Food Science and human Nutrition Chonbuk National UniversityJeonju561-756Republic of Korea Department of Food and Life Science Pukyong National UniversityBusan 608-737Republic of Korea Institute of New Frontier Stroke Research Team College of MedicineHallym UniversityChuncheon24252Republic of Korea Department of Neurosurgery College of MedicineHallym UniversityChuncheon24252Republic of Korea Graduate School of Science and Engineering for Research University of ToyamaToyama930-8555Japan

Inhibition ofβ-site amyloid precursor protein-cleaving enzyme 1(BACE1)or glycogen synthase kinase-3β(GSk-3β)is estimated to be the central therapeutic approach for Alzheimer’s disease(AD).In this study,water extract of kangenkaryu,its crude drug and chemical composition used in oriental medicine were evaluated regarding their BACE1 and GSk-3βinhibitory *** resonance energy transfer was used to characterize the BACE1 inhibitory effect of kangen-karyu,its crude drug and chemical ***-3βactivity was determined using the kinase-Glo Luminescent kinase Assay *** water extract of kangen-karyu inhibited BACE1 and GSk-3βin concentration-dependent manners when compared with reference drugs,quercetin and *** six components of kangen-karyu,the water extracts of Salviae Miltiorrhizae Radix or Cyperi Rhizoma exhibited significant inhibitory effects on BACE1 and GSk-3β.Among the constituents of Salviae Miltiorrhizae Radix extract,salvianolic acid C,salvianolic acid A,rosmarinic acid,and magnesium lithospermate B significantly inhibited *** addition,they inhibited GSk-3βwith an IC50 value range of 6.97 to 135.35μ*** these results,one of the effectiveness and its mechanisms of action of kangen-karyu against AD may be the inhibition of BACE1 and GSk-3β,and one of the active ingredients of kangen-karyu is Salviae Miltiorrhizae Radix and its constituents.

关键词： Alzheimer’s disease β-Site amyloid precursor protein-cleaving enzyme 1 Glycogen synthase kinase-3β kangen-karyu Salviae Miltiorrhizae Radix Salvianolic acid C Salvianolic acid B

Protein kinase A-mediated phosphorylation of HERG potassium channels in a human cell line

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华医学杂志(英文版) 2002年第5期000卷 28-36页

作者： wEI Zhang, Dierk Thomas, Christoph A karle, Sven kathfer, Johannes Schenkel, Volker A w kreye, Eckhard Ficker Barbara A wible and Johann kiehn Department of Cardiology, Medical University Hospital Heidelberg, Heidelberg, Germany (Zhang w, T Department of Cardiology Medical University Hospital Heidelberg Heidelberg Germany Department of Physiology and Pathophysiology University of HeidelbergHeidelberg Germany Rammelkamp Center for Education and Research Case Western Reserve University School of Medicine MetroHealth Medical Center Cleveland USA

Objective To investigate the molecular mechanism of human ether a go go related gene (HERG) potassium channels regulated by protein kinase A (PkA) in a human cell line Methods HERG channels were stably expressed in human embryonic kidney (HEk) 293 cells, and currents were measured with the patch clamp technique The direct phosphorylation of HERG channel proteins expressed heterologously in Xenopus laevis oocytes was examined by 32 P labeling and immunoprecipitation with an anti HERG antibody Results Elevation of the intracellular cAMP concentration by incubation with the adenylate cyclase activator, forskolin (10?μmol/L), and the broad range phosphodiesterase inhibitor, IBMX (100?μmol/L), caused a HERG tail current reduction of 83 2% In addition, direct application of the membrane permeable cAMP analog, 8 Br cAMP (500?μmol/L), reduced the tail current amplitude by 29 3% Intracellular application of the catalytic subunit of protein kinase A (200 U/ml) led to a tail current decrease by 56 9% and shifted the activation curve by 15 4 mV towards more positive potentials HERG wT proteins showed two phosphorylated bands, an upper band with a molecular mass of ≈155 kDa and a lower band with a molecular mass of ≈135 kDa, indicating that both the core and the fully glycosylated forms of the protein were phosphorylated Conclusions PkA mediated phosphorylation of HERG channels causes current reduction in a human cell line The coupling between the repolarizing cardiac HERG potassium current and the protein kinase A system could contribute to arrhythmogenesis under pathophysiological conditions

关键词： adrenergic stimulation · arrhythmia · cyclic AMP · HERG potassium channel · protein kinase A

Effect of co-presence of NSAIDs with cadmium:i)evaluation of NSAIDbearing water for washing Cd from soil,ii)Cd removal from NSAIDbearing water using magnetic graphene oxide

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Emerging Contaminants 2022年第1期8卷 151-161页

作者： Amjad H.El-Sheikh Haneen k.Abu-Dgham Mahmoud S.Sunjuk Nabil N.Al-Hashimi Department of Chemistry Faculty of ScienceThe Hashemite UniversityP.O.Box 330127Zarqa13133Jordan Faculty of Pharmacy The Hashemite UniversityP.O.Box 330127Zarqa13133Jordan

Cadmium“Cd”is a toxic pollutant that may present in soil and *** work evaluates:i)the use of non-steroidal anti-inflammatory drugs“NSAIDs”-bearing water for washing soil containing Cd(Ⅱ),ii)removal of Cd(Ⅱ)from NSAID-bearing water by adsorption onto magnetic graphene oxide which can be easily separated by strong *** studied NSAIDs are aspirin,ketoprofen,ibuprofern and *** Cd(Ⅱ)-NSAIDs complexes were synthesized and characterized by *** was initially oxidized by either nitric acid,or ammonium persulphate method,or hummer's *** was then deposited on graphene oxide to give the corresponding magnetic graphene oxides(NA-MGO,APSMGO and hum-MGO,respectively).The effect of the following factors on Cd(Ⅱ)uptake was investigated:NSAIDs type,pH,graphene oxidation method,magnetite:graphene oxide mass ratio in the adsorbent,(Cd(Ⅱ):NSAID)molar *** Cd(Ⅱ)uptake was achieved using“magnetic graphene oxidized with ammonium persulphate where the mass ratio of magnetite to graphene oxide was 2:1”in the presence of diclofenac at *** best Cd(Ⅱ):diclofenac molar ratio was 1:*** maximum adsorption capacity of Cd(Ⅱ)was found to be 83 mg L*** regeneration of the adsorbent was possible by 0.3 M HNO3 solution and 80%of adsorption efficiency was maintained after five *** presence of coexisting ions,80%of the adsorption efficiency was *** NSAIDs-containing waters were used for washing Cd-containing soil;the maximum removal efficiencies of Cd were 18%and 16%using 5 mM diclofenac or 10 mM aspirin,*** diclofenac or aspirin-spiked real pharmaceutical wastewater gave 28%removal of *** optimum adsorption method was used for removal of Cd(Ⅱ)from diclofenac-containing soil-washing water,where two successive adsorptions were needed for complete Cd uptake.

关键词： Adsorption Cadmium Magnetic graphene oxide NSAIDs Pharmaceutical wastewater Soil washing

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

糖蛋白聚糖的功能

氨基酸和生物资源 1985年第4期13卷 64-68页

作者： kenneth Olden Bruno A.Bernard Martin J.hum-phries Tet-kin Yeo kiang-Teck Yeo Sandra L.white Sheila A.Newton Hans C.Bauer J.Brian Parent 王鄂生

近来几个实验室同时获得的结果已被评论,它增强了早期的见解,糖蛋白聚糖部分在(1)维持蛋白质的构象和稳定性,(2)肽的蛋白(酶)水解加工和对抗非控制性的蛋白(酶)水解的稳定性,(3)生物活性的中介作用,(4)糖蛋白在细胞内的区分与外在化(ext... 详细信息

近来几个实验室同时获得的结果已被评论,它增强了早期的见解,糖蛋白聚糖部分在(1)维持蛋白质的构象和稳定性,(2)肽的蛋白(酶)水解加工和对抗非控制性的蛋白(酶)水解的稳定性,(3)生物活性的中介作用,(4)糖蛋白在细胞内的区分与外在化(externalization),(5)胚胎发育与分化等方面起着重要的作用。

关键词：蛋白聚糖核糖核酸酶胚胎发育非糖基化蛋白解的稳定性外在化胞内运输溶酶体粗面内质网生物活性

SIRT3过表达通过调控细胞代谢转换抑制肾肿瘤细胞的增殖

在线全文

学校读者我要写书评

暂无评论

现代生物医学进展 2019年第5期19卷 801-806页

作者：刘欢陈宇凌徐仁华许嘉桐邓海腾清华大学生命科学学院教育部生物信息学重点实验室北京100084 滨州医学院护理学院山东烟台264003

目的:研究SIRT3对肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)769-P细胞增殖和抗氧化能力的影响,并进一步探究其作用机制。方法:在769-P细胞的基础上构建SIRT3过表达稳转细胞系;利用CCk-8试剂检测769-P SIRT3过表达细胞的增... 详细信息

目的:研究SIRT3对肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)769-P细胞增殖和抗氧化能力的影响,并进一步探究其作用机制。方法:在769-P细胞的基础上构建SIRT3过表达稳转细胞系;利用CCk-8试剂检测769-P SIRT3过表达细胞的增殖速度;利用CellROX~Deep Red染料并结合流式细胞分析检测SIRT3过表达对769-P细胞中ROS水平的影响;利用定量蛋白质组学和代谢组学的方法,探究SIRT3对769-P细胞的作用机制。结果:CCk-8实验结果表明,769-P SIRT3过表达细胞的生长速度与对照细胞相比下降了约48%;定量蛋白质组学分析显示,769-P SIRT3过表达细胞中ALDOA、ALDOA、ENO2、PkM、LDHA、LDHB表达量下调约0.4至0.7倍,SDHB和CS上调约1.3倍;代谢组学分析显示,PEP、pyruvic acid、lactate、carnitine水平下降约0.4至0.7倍,isocitric acid和acetyl-CoA水平升高分别约1.3和2.8倍;分析还显示SIRT3过表达上调SOD2、TXN、GPX4和GLRX5的表达量约1.3至2倍,降低ROS水平约40%,增强细胞对过氧化氢的耐受力。结论:SIRT3过表达引起769-P细胞的代谢转换,从而抑制其增殖;且上调769-P细胞中抗氧化酶的表达,降低ROS水平,增强细胞的抗氧化能力。

关键词： SIRT3 肾透明细胞癌定量蛋白质组学代谢组学

Spatial distribution and seasonal variation of char-EC and soot-EC in the atmosphere over China

在线全文

学校读者我要写书评

暂无评论

Spatial distribution and seasonal variation of char-EC and s...

尿道多重耐药菌定植与老年前列腺增生症术后尿路感染关联性分析

第五届海峡两岸气溶胶技术研讨会

作者： S.C.Lee k.F.Ho Research Center of Urban Environmental Technology and Management Department of Civil and Structural EngineeringThe Hong Kong Polytechnic UniversityHung HumKowloonHong Kong

EC is a collective term that encompasses all thermally altered carbonaceous material generated from fossil fuel combustion and biomass burning,and can be subdivided into two classes: soot and *** study on PM carbonaceous aerosols (Cao et al.,2007,***.,112, D22S11,doi:10.1029/2006JD008205,2007) measured with the thermal optical reflectance (TOR) method in fourteen Chinese cities are refined by subdividing total EC into char-EC and soot-EC. Average char-EC concentrations vary greatly among the fourteen cities and for winter and summer periods,with concentrations of 8.67 and 2.41 μg m in winter and summer,respectively;while spatial and seasonal soot-EC vary small,with average concentrations of 1.26 and 1.21 μg m in winter and summer,*** to EC,spatial distributions of char-EC are influenced by the fuel consumptions and the Asian monsoon *** small spatial and seasonal variation of soot-EC is consistent with its regional-to-global *** correlations between char-EC and total EC suggest that EC concentrations may be determined mainly by char-EC *** primary emission particles,char-EC and soot-EC ratio is good indicator of EC source profiles since it varies by different source ***-EC/soot-EC ratios show summer minima and winter maxima in all these cities,which are consistent with the fuel *** correlation between char-EC/soot-EC and OC/EC,as well as seasonal small variation of OC/EC,suggests that OC/EC may not be good indicator for EC source identification.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

中国消毒学杂志 2019年第3期36卷 206-208页

作者：谢朝云蒙桂鸾熊芸谢强杨忠玲陈应强杨怀贵州医科大学第三附属医院贵州都匀558000 贵州省人民医院

目的验证老年良性前列腺增生症患者尿道多重耐药菌定植与术后尿路感染的关联性。方法采用巢式病例对照研究,对2014年2月-2018年1月某医院前列腺增生症老年患者的术后尿路感染发生情况进行调查,比较病例组与对照组间尿道多重耐药菌(MDROS... 详细信息

目的验证老年良性前列腺增生症患者尿道多重耐药菌定植与术后尿路感染的关联性。方法采用巢式病例对照研究,对2014年2月-2018年1月某医院前列腺增生症老年患者的术后尿路感染发生情况进行调查,比较病例组与对照组间尿道多重耐药菌(MDROS)定植率差异;分析前列腺增生症术后尿路感染主要危险因素及其与MDROs的相关性。结果共调查术后尿路感染病例278例,与556例对照组相比,其尿道MDROS定植率明显增高,总住院时间和术后住院时间均较长;尿道MDROs定植与术后尿路感染风险呈正相关(OR=1. 599,95%CI:1. 004～2. 546)。结论存在尿道MDROs定植的老年良性前列腺增生症患者术后尿路感染发生风险较大,需重视抗菌药物的选择。

关键词：良性前列腺增生症手术部位感染多重耐药菌定植

刺玫果提取物总黄酮含量以及体外抗氧化活性的测定

在线全文

学校读者我要写书评

暂无评论

化学工程师 2018年第11期32卷 26-28页

作者：孟永海怀雪翟春梅王知斌陈忠新黑龙江中医药大学黑龙江哈尔滨15004

测定刺玫果提取物总黄酮含量及抗氧化活性。方法:以芦丁为对照品测得刺玫果水提物和醇提物总黄酮含量,利用DPPH自由基抗氧化活性指数确定刺玫果水提物和醇提物的清除自由基能力。结果:刺玫果水提物总黄酮含量为96.4mg·g-1;刺玫果醇提... 详细信息

测定刺玫果提取物总黄酮含量及抗氧化活性。方法:以芦丁为对照品测得刺玫果水提物和醇提物总黄酮含量,利用DPPH自由基抗氧化活性指数确定刺玫果水提物和醇提物的清除自由基能力。结果:刺玫果水提物总黄酮含量为96.4mg·g-1;刺玫果醇提物总黄酮含量为220.6mg·g-1。刺玫果水提物自由基清除率IC50为1.323;醇提物IC50为0.078。结论:刺玫果水提物和醇提物总黄酮含量及其抗氧化活性存在较大差距。刺玫果醇提物总黄酮含量高于水提物;且醇提物抗氧化活性优于水提物。

关键词：刺玫果总黄酮抗氧化活性