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A domesticated Harbinger transposase forms a complex with HDA6 and promotes histone H3 deacetylation at genes but not TEs in Arabidopsis

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Journal of Integrative Plant Biology 2021年第8期63卷 1462-1474页

作者： Xishi Zhou Junna He Christos N.Velanis Yiwang Zhu Yuhan He Kai Tang Mingku Zhu Lisa Graser Erica de Leau Xingang wang Lingrui Zhang w.andy tao Justin Goodrich Jian-Kang Zhu Cui-Jun Zhang Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen518124 China College of Horticulture China Agricultural UniversityBeijing100193 China Department of Horticulture and Landscape Architecture Purdue UniversityWest LafayetteIN 47907 USA Institute of Molecular Plant Science School of Biological SciencesUniversity of EdinburghDaniel Rutherford BuildingMax Born CrescentEdinburghEH93BF United Kingdom Shanghai Center for Plant Stress Biology and CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of SciencesShanghai201602 China Institute of Integrative Plant Biology School of Life SciencesJiangsu Normal UniversityXuzhou221116 China University of Applied Sciences Mannheim Paul-Wittsack-Str.10Mannheim68163 Germany Department of Biochemistry Purdue UniversityWest LafayetteIN 47907 USA

In eukaryotes,histone acetylation is a major modification on histone N-terminal tails that is tightly connected to transcriptional ***6 is a histone deacetylase involved in the transcriptional regulation of genes and transposable elements(TEs)in Arabidopsis ***6 has been shown to participate in several complexes in plants,including a conserved SIN3 ***,we uncover a novel protein complex containing HDA6,several Harbinger transposon-derived proteins(HHP1,SANT1,SANT2,SANT3,and SANT4),and MBD domain-containing proteins(MBD1,MBD2,and MBD4).we show that mutations of all four SANT genes in the sant-null mutant cause increased expression of the flowering repressors FLC,MAF4,and MAF5,resulting in a late flowering *** deep sequencing reveals that while the SANT proteins and HDA6 regulate the expression of largely overlapping sets of genes,TE silencing is unaffected in sant-null *** global histone H3 acetylation profiling shows that SANT proteins and HDA6 modulate gene expression through ***,our findings suggest that Harbinger transposon-derived SANT domain-containing proteins are required for histone deacetylation and flowering time control in plants.

关键词： deacetylation flowering Harbinger HDA6 histone acetylation protein complex SANT

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BNIP3 Suppresses PINK1 Proteolytic Cleavage to Promote Mitophagy

BNIP3 Suppresses PINK1 Proteolytic Cleavage to Promote Mitop...

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第十四次全国医学遗传学学术会议

作者： Zhang TM Xue L Li L Tang CY wan ZQ wang RX Zeng ZT Tan JQ Tang BS w.andy tao Zhang ZH The State Key Laboratory of Medical Genetics Xiangya Medical SchoolCentral South University Department of Biochemistry Purdue University Department of Neurology Xiangya HospitalCentral South University Collaborative Innovation Center for Brain Science Fudan University

Objective:Mutations in PINK1 gene cause recessive form of Parkinson’s disease(PD).The PINK1 gene encodes a mitochondrial protein kinase that is processed by mitochondrial proteases resulting in two smaller proteolytic *** not only protects cells against mitochondrial dysfunction and apoptosis,but also regulates clearance of damaged mitochondria together with ***2/adenovirus E1B 19kDa interacting protein 3(BNIP3)is a mitochondrial BH3-only protein known to induce *** present study is to explore whether PINK1/Parkin and BNIP3 function in the same pathway to regulate mitochondria ***:The interaction between PINK1 and BNIP3was determined by co-immunoprecipition *** induction was analyzed with parkin and GFP-LC3 ***:Our results reveal that BNIP3 interacts with PINK1 to promote stabilization and accumulation of full-length PINK1 on outer membrane of damaged mitochondria,therefore,to facilitate parkin recruitment to ***:The results suggest that BNIP3 plays an essential role in PINK1/parkinmediated mitophagy and likely contributes to PD pathogenesis.

关键词： Parkinson’s disease PINK1 BNIP3 mitophagy

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Mapping Proteome-wide Targets of Protein Kinases in Plant Stress Responses

Mapping Proteome-Wide Targets of Protein Kinases in Plant St...

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2018全国植物生物学大会

作者： Pengcheng wang Chuan-Chih Hsu Yanyan Du Peipei Zhu Chunzhao Zhao Xing Fu Chunguang Zhang Juan Sebastian Paez Alberto P.Macho w.andy tao Jian-Kang Zhu Shanghai Center for Plant Stress Biology CASCenter for Excellence in Molecular Plant SciencesChinese Academy of Sciences Department of Biochemistry Purdue University Department of Horticulture and Landscape Architecture Purdue University

Protein kinases are major regulatory components in almost all cellular processes in eukaryotic *** adding phosphate groups,protein kinases regulate the activity,localization,protein-protein interactions,and other features of their target *** is known that protein kinases,such as SNF1-related protein kinases 2 and 3(SnRK2 and SnRK3),calcium dependent protein kinases(CDPKs),and mitogen activated protein kinases(MAPKs) are central components in plant response to environmental stresses,such as drought,high salinity,cold,and pathogen *** phosphorylationdependent signaling plays critical roles in plant stress biology,only a small number of targets of these protein kinases have been identified,and consequently how these protein kinases regulate downstream biological processes and mediate stress responses are still largely *** this study,we developed an isotope labeled protein kinase assay-based approach with enriched phosphoproteomes as kinase-substrate pools to identify the putative substrates of eight protein kinases that function in plant abiotic and biotic stress *** a result,we identified more than five thousand putative targets from 11 protein kinase-stimuli *** also analyzed the conserved motif and functional ontology of these putative kinase *** results provide comprehensive information on the role of these protein kinases in controlling cellular activities and constitute a valuable resource for further studies on the mechanisms underlying plant response to environmental stresses.

关键词： stress phosphoproteomics kinase substrate MPK6 SnRK2 CDPK

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Inhibiting SHP2 reduces glycolysis, promotes microglial M1 polarization, and alleviates secondary inflammation following spinal cord injury in a mouse model

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Neural Regeneration Research 2025年第3期20卷 858-872页

作者： Xintian Ding Chun Chen Heng Zhao Bin Dai Lei Ye tao Song Shuai Huang Jia wang tao You Department of Orthopedics The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Orthopedics Provincial Hospital Affiliated to Anhui Medical UniversityHefeiAnhui ProvinceChina

Reducing the secondary inflammatory response, which is partly mediated by microglia, is a key focus in the treatment of spinal cord injury. Src homology 2-containing protein tyrosine phosphatase 2(SHP2), encoded by PTPN11, is widely expressed in the human body and plays a role in inflammation through various mechanisms. Therefore, SHP2 is considered a potential target for the treatment of inflammation-related diseases. However, its role in secondary inflammation after spinal cord injury remains unclear. In this study, SHP2 was found to be abundantly expressed in microglia at the site of spinal cord injury. Inhibition of SHP2 expression using siRNA and SHP2 inhibitors attenuated the microglial inflammatory response in an in vitro lipopolysaccharide-induced model of inflammation. Notably, after treatment with SHP2 inhibitors, mice with spinal cord injury exhibited significantly improved hind limb locomotor function and reduced residual urine volume in the bladder. Subsequent in vitro experiments showed that, in microglia stimulated with lipopolysaccharide, inhibiting SHP2 expression promoted M2 polarization and inhibited M1 polarization. Finally, a co-culture experiment was conducted to assess the effect of microglia treated with SHP2 inhibitors on neuronal cells. The results demonstrated that inflammatory factors produced by microglia promoted neuronal apoptosis, while inhibiting SHP2 expression mitigated these effects. Collectively, our findings suggest that SHP2 enhances secondary inflammation and neuronal damage subsequent to spinal cord injury by modulating microglial phenotype. Therefore, inhibiting SHP2 alleviates the inflammatory response in mice with spinal cord injury and promotes functional recovery postinjury.

关键词： apoptosis glycolysis inflammatory response microglia neurons polarization spinal cord injury Src homology 2-containing protein tyrosine phosphatase 2

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MAP激酶级联通过调节ICE1蛋白质稳定性来调节冷应答

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科学新闻 2018年第4期 102-102页

作者： Chunzhao Zhao Pengcheng wang Tong Si Chuan-Chih Hsu Lu wang Omar Zayed Zheping Yu Yingfang Zhu Juan Dong w.andy tao 朱健康中国科学院上海植物逆境生物学研究中心

文章简介丝裂原活化蛋白激酶(MAPK)级联信号通路是将环境信号转化为细胞应答反应的重要信号模块。本研究发现,冷处理后MPK3、MPK4和MPK6可被迅速激活。mpk3和mpk6突变体的CBF基因表达升高及耐寒性增强,而组成型激活植物MKK4/5-MPK3/6信... 详细信息

文章简介丝裂原活化蛋白激酶(MAPK)级联信号通路是将环境信号转化为细胞应答反应的重要信号模块。本研究发现,冷处理后MPK3、MPK4和MPK6可被迅速激活。mpk3和mpk6突变体的CBF基因表达升高及耐寒性增强,而组成型激活植物MKK4/5-MPK3/6信号级联则导致CBF基因表达

关键词：基因表达 MAP

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Current technologies to identify protein kinase substrates in high throughput

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Frontiers in Biology 2013年第2期8卷 216-227页

作者： Liang XUE w. andy tao Department of Biochemistry Purdue University West Lafayette IN 47907 USA Department of Medicinal Chemistry & Molecular Pharmacology Purdue University West Lafayette IN 47907 USA Department of Chemistry Purdue University West Lafayette IN 47907 USA Purdue University Center for Cancer Research Purdue University West Lafayette IN 47907 USA

Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.

关键词： phosphorylation kinase substrate in vitro kinase assay high throughput screening mass spectrometry,phosphoproteomics

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Preparation of FeCoNi medium entropy alloy from Fe^(3+)-Co^(2+)-Ni^(2+)solution system

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 92-101页

作者： Zongyou Cheng Qing Zhao Mengjie tao Jijun Du Xingxi Huang Chengjun Liu Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China Engineering Research Center of Frontier Technologies for Low-carbon Steelmaking(Ministry of Education) Shenyang 110819China

In recent years,medium entropy alloys have become a research hotspot due to their excellent physical and chemical *** controlling reasonable elemental composition and processing parameters,the medium entropy alloys can exhibit similar properties to high entropy alloys and have lower *** this paper,a FeCoNi medium entropy alloy precursor was prepared via sol-gel and coprecipitation methods,respectively,and FeCoNi medium entropy alloys were prepared by carbothermal and hydrogen *** phases and magnetic properties of FeCoNi medium entropy alloy were *** showed that FeCoNi medium entropy alloy was produced by carbothermal and hydrogen reduction at 1500℃.Some carbon was detected in the FeCoNi medium entropy alloy prepared by carbothermal *** alloy prepared by hydrogen reduction was uniform and showed a relatively high ***,the hydrogen reduction product exhibited better saturation magnetization and lower coercivity.

关键词： medium entropy alloy sol-gel co-precipitation carbothermal hydrogen reduction

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Targeted gene sequencing and bioinformatics analysis of patients with gallbladder neuroendocrine carcinoma:A case report

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world Journal of Gastrointestinal Oncology 2025年第1期17卷 239-251页

作者： Yun-Chuan Yang Zhi-tao Chen Da-Long wan Hui Tang Mu-Lin Liu Department of Medical College Jinan UniversityGuangzhou 510000Guangdong ProvinceChina Department of General Surgery The First Affiliated Hospital of Bengbu Medical UniversityBengbu 233000Anhui ProvinceChina Department of Hepatobiliary Surgery Shulan(Hangzhou)Hospital Affiliated to Zhejiang Shuren University Shulan International Medical CollegeHangzhou 310022Zhejiang ProvinceChina Department of Hepatobiliary and Pancreatic Surgery The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Pathology The First Affiliated Hospital of Medical School of Zhejiang UniversityHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Gallbladder neuroendocrine carcinoma(NEC)represents a subtype of gallbladder malignancies characterized by a low incidence,aggressive nature,and poor *** its clinical severity,the genetic alterations,mechanisms,and signaling pathways underlying gallbladder NEC remain *** SUMMARY This case study presents a rare instance of primary gallbladder NEC in a 73-year-old female patient,who underwent a radical cholecystectomy with hepatic hilar lymphadenectomy and resection of liver segments IV-B and *** gene sequencing and bioinformatics analysis tools,including STRING,GeneMANIA,Metascape,TRRUST,Sangerbox,cBioPortal and GSCA,were used to analyze the biological functions and features of mutated genes in gallbladder *** mutations(APC,ARID2,IFNA6,KEAP1,RB1,SMAD4,TP53,BTK,GATA1,GNAS,and PRDM3)were identified,and the tumor mutation burden was determined to be 9.52 muts/Mb via targeted gene sequencing.A protein-protein interaction network showed significant interactions among the twelve mutated *** Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were used to assess mutation functions and *** results revealed 40 tumor-related pathways.A key regulatory factor for gallbladder NEC-related genes was identified,and its biological functions and features were compared with those of gallbladder *** Gallbladder NEC requires standardized *** with other gallbladder carcinomas revealed clinical phenotypes,molecular alterations,functional characteristics,and enriched pathways.

关键词： Gallbladder neuroendocrine carcinoma Targeted-gene sequencing Bioinformatics analysis case report Immunocytochemistry Case report

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Spatial transcriptomics combined with single-nucleus RNA sequencing reveals glial cell heterogeneity in the human spinal cord

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Neural Regeneration Research 2025年第11期20卷 3302-3316页

作者： Yali Chen Yiyong wei Jin Liu tao Zhu Cheng Zhou Donghang Zhang Department of Anesthesiology West China HospitalSichuan UniversityChengduSichuan ProvinceChina Laboratory of Anesthesia and Critical Care Medicine National-Local Joint Engineering Research Center of Translational Medicine of AnesthesiologyWest China HospitalSichuan UniversityChengduSichuan ProvinceChina Department of Anesthesiology Longgang District Maternity&Child Healthcare Hospital of Shenzhen City(Longgang Maternity and Child Institute of Shantou University Medical College)ShenzhenGuangdong ProvinceChina Department of Anesthesiology Affiliated Hospital of Zunyi Medical UniversityZunyiGuizhou ProvinceChina

Glial cells play crucial roles in regulating physiological and pathological functions,including sensation,the response to infection and acute injury,and chronic neurodegenerative *** cells include astrocytes,microglia,and oligodendrocytes in the central nervous system,and satellite glial cells and Schwann cells in the peripheral nervous *** the greater understanding of glial cell types and functional heterogeneity achieved through single-cell and single-nucleus RNA sequencing in animal models,few studies have investigated the transcriptomic profiles of glial cells in the human spinal ***,we used high-throughput single-nucleus RNA sequencing and spatial transcriptomics to map the cellular and molecular heterogeneity of astrocytes,microglia,and oligodendrocytes in the human spinal *** explore the conservation and divergence across species,we compared these findings with those from *** the human spinal cord,astrocytes,microglia,and oligodendrocytes were each divided into six distinct transcriptomic *** the mouse spinal cord,astrocytes,microglia,and oligodendrocytes were divided into five,four,and five distinct transcriptomic subclusters,*** comparative results revealed substantial heterogeneity in all glial cell types between humans and ***,we detected sex differences in gene expression in human spinal cord glial ***,in all astrocyte subtypes,the levels of NEAT1 and CHI3L1 were higher in males than in females,whereas the levels of CST3 were lower in males than in *** all microglial subtypes,all differentially expressed genes were located on the sex *** addition to sex-specific gene differences,the levels of MT-ND4,MT2A,MT-ATP6,MT-CO3,MT-ND2,MT-ND3,and MT-CO_(2) in all spinal cord oligodendrocyte subtypes were higher in females than in ***,the present dataset extensively characterizes glial cell heterogeneity and offers a valuable resource for explo

关键词： astrocyte glial cell human microglia oligodendrocyte sex differentiation single-nucleus RNA sequencing spatial transcriptomics species variation spinal cord

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Cholesterol metabolism: physiological versus pathological aspects in intracerebral hemorrhage

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Neural Regeneration Research 2025年第4期20卷 1015-1030页

作者： Ruoyu Huang Qiuyu Pang Lexin Zheng Jiaxi Lin Hanxi Li Lingbo wan tao wang Department of Forensic Science School of Basic Medicine and Biological SciencesSuzhou Medicine College of Soochow UniversitySuzhouJiangsu ProvinceChina

Cholesterol is an important component of plasma membranes and participates in many basic life functions,such as the maintenance of cell membrane stability,the synthesis of steroid hormones,and *** plays a key role in the establishment and maintenance of the central nervous *** brain contains 20%of the whole body’s cholesterol,80%of which is located within myelin.A huge number of processes(e.g.,the sterol regulatory element-binding protein pathway and liver X receptor pathway)participate in the regulation of cholesterol metabolism in the brain via mechanisms that include cholesterol biosynthesis,intracellular transport,and *** brain injuries or diseases involving crosstalk among the processes above can affect normal cholesterol metabolism to induce detrimental ***,we hypothesized that cholesterol-related molecules and pathways can serve as therapeutic targets for central nervous system *** hemorrhage is the most severe hemorrhagic stroke subtype,with high mortality and *** cholesterol levels are associated with the risk of intracerebral ***,secondary pathological changes after intracerebral hemorrhage are associated with cholesterol metabolism dysregulation,such as neuroinflammation,demyelination,and multiple types of programmed cell *** cholesterol accumulation in the brain has been found after intracerebral *** this paper,we review normal cholesterol metabolism in the central nervous system,the mechanisms known to participate in the disturbance of cholesterol metabolism after intracerebral hemorrhage,and the links between cholesterol metabolism and cell *** also review several possible and constructive therapeutic targets identified based on cholesterol metabolism to provide cholesterol-based perspectives and a reference for those interested in the treatment of intracerebral hemorrhage.

关键词： cell death cholesterol metabolism intracerebral hemorrhage myelination therapeutic target

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