Current technologies to identify protein kinase substrates in high throughput
Current technologies to identify protein kinase substrates in high throughput作者机构:Department of Biochemistry Purdue University West Lafayette IN 47907 USA Department of Medicinal Chemistry & Molecular Pharmacology Purdue University West Lafayette IN 47907 USA Department of Chemistry Purdue University West Lafayette IN 47907 USA Purdue University Center for Cancer Research Purdue University West Lafayette IN 47907 USA
出 版 物:《Frontiers in Biology》 (生物学前沿(英文版))
年 卷 期:2013年第8卷第2期
页 面:216-227页
核心收录:
学科分类:1305[艺术学-设计学(可授艺术学、工学学位)] 0710[理学-生物学] 13[艺术学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 081104[工学-模式识别与智能系统] 08[工学] 0817[工学-化学工程与技术] 0804[工学-仪器科学与技术] 081101[工学-控制理论与控制工程] 0811[工学-控制科学与工程]
基 金:in part by National Institutes of Health by National Institute of Food and Agriculture (NIFA)
主 题:phosphorylation kinase substrate in vitro kinase assay high throughput screening mass spectrometry,phosphoproteomics
摘 要:Since the discovery of protein phosphorylation as an important modulator of many cellular processes, the involvement of protein kinases in diseases, such as cancer, diabetes, cardiovascular diseases, and central nervous system pathologies, has been extensively documented. Our understanding of many disease pathologies at the molecular level, therefore, requires the comprehensive identification of substrates targeted by protein kinases. In this review, we focus on recent techniques for kinase substrate identification in high throughput, in particular on genetic and proteomic approaches. Each method with its inherent advantages and limitations is discussed.