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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2012年第2期13卷 83-93页

作者： Hui ZHANG Lu ZHANG Li-juan PENG Xiao-wu DONG Di wu vivian chi-hua wu Feng-qin FENG Department of Food Science and Nutrition Zhejiang UniversityHangzhou 310058China ZJU-ENS Joint Laboratory of Medicinal Chemistry College of Pharmaceutical SciencesZhejiang UniversityHangzhou 310058China Department of Food Science and Human Nutrition University of MaineOrono 04469USA

Fatty acids and derivatives(FADs)are resources for natural *** order to screen for additional potent antimicrobial agents,the antimicrobial activities of FADs against Staphylococcus aureus were examined using a microplate *** of fatty acids were the most potent class of fatty acids,among which monotridecanoin possessed the most potent antimicrobial *** conventional quantitative structure-activity relationship(QSAR)and comparative molecular field analysis(CoMFA)were performed to establish two statistically reliable models(conventional QSAR:R2=0.942,Q 2 LOO=0.910;CoMFA:R 2=0.979,Q 2=0.588,respectively).Improved forecasting can be achieved by the combination of these two models that provide a good insight into the structureactivity relationships of the FADs and that may be useful to design new FADs as antimicrobial agents.

关键词： Fatty acid derivatives Quantitative structure-activity relationship Comparative molecular field analysis Antimicrobial activity

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牛蒡提取物抗氧化活性及抑菌作用研究

食品工业科技 2010年第6期31卷 131-133页

作者：贺菊萍梁思赵勇孙晓红 vivian chi-hua wu 潘迎捷上海海洋大学食品学院上海201306 徐州工程学院食品工程学院江苏徐州221008 美国缅因大学食品科学与人类营养系美国缅因州044695735

分别采用1,1-二苯基苦基苯肼(DPPH)和羟基自由基的清除效果以及还原力生成体系,研究了牛蒡皮乙醇提取物(PA)、牛蒡皮水提取物(PW)、牛蒡肉乙醇提取物(MA)和牛蒡肉水提取物(MW)的抗氧化性;以纸片扩散法和最小抑菌浓度的确定研究了四种处... 详细信息

分别采用1,1-二苯基苦基苯肼(DPPH)和羟基自由基的清除效果以及还原力生成体系,研究了牛蒡皮乙醇提取物(PA)、牛蒡皮水提取物(PW)、牛蒡肉乙醇提取物(MA)和牛蒡肉水提取物(MW)的抗氧化性;以纸片扩散法和最小抑菌浓度的确定研究了四种处理方式所得提取物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、黑曲霉和酵母菌的抑菌性能。结果显示,四种处理方式所得牛蒡提取物均具有一定的抗氧化作用,其中PA提取物抗氧化性最好;PA、PW和MA具有较好的抑菌效果,PA抑菌效果最好。

关键词：牛蒡抗氧化抑菌

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牛蒡皮提取液对猪肉中微生物的抑制作用

农业工程学报 2011年第S2期27卷 242-246页

作者：贺菊萍赵勇孙晓红 vivian chi-hua wu 唐晓阳潘迎捷上海海洋大学食品学院上海201306 徐州工程学院食品工程学院徐州221008 Department of Food science and Human Nutrition University of Mainemaine044695735

为了研究牛蒡皮提取液对猪肉中微生物的抑制作用,采用体积分数为250、500、750、1 000μL/mL牛蒡皮提取液(每1 mL原液由500 mg牛蒡皮提取制的)分别处理新鲜及接种单增李斯特菌猪肉,之后将处理好的猪肉分别置于25℃和4℃培养,于0、12、24... 详细信息

为了研究牛蒡皮提取液对猪肉中微生物的抑制作用,采用体积分数为250、500、750、1 000μL/mL牛蒡皮提取液(每1 mL原液由500 mg牛蒡皮提取制的)分别处理新鲜及接种单增李斯特菌猪肉,之后将处理好的猪肉分别置于25℃和4℃培养,于0、12、24、48 h(25℃)和0、1、3、5、7 d(4℃)测定其中菌落总数和单增李斯特菌数量;对25℃条件下经各浓度提取液处理24 h后猪肉的感官质量进行了评价;透射电镜观察了25℃条件下经500μL/mL牛蒡提取液处理7h后单增李斯特菌细胞形态的变化。结果显示,与对照组相比较,25℃条件下经500、750、1000μL/mL牛蒡皮提取液处理后,猪肉中菌落总数和单增李斯特菌均显著(P<0.05)降低,抑菌效果和提取液浓度呈正相关;牛蒡皮提取液浓度为500μL/mL时猪肉感官质量较佳;牛蒡提取液对单增李斯特菌的抑制表现为对其细胞壁和胞内物质的破坏。本研究为今后牛蒡提取液作为天然食品添加剂在食品中的应用提供参考。

关键词：农业废弃物微生物食品添加剂牛蒡猪肉菌落总数单增李斯特菌抑菌感官评价电镜分析

环介导恒温扩增技术可视化检测携带tdh基因的致病性副溶血性弧菌

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食品工业科技 2014年第24期35卷 71-75页

作者：陈梅萍孙晓红姜文洁赵勇 vivian chi-hua wu 潘迎捷上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心农业部水产品贮藏保鲜质量安全风险评估实验室(上海)上海201306 美国缅因大学食品与农业系美国缅因州04469

建立了环介导恒温扩增技术检测携带tdh基因的致病性副溶血性弧菌。基于副溶血性弧菌高度保守的tdh基因序列,设计了6条特异性引物,两条外引物F3、B3,两条内引物FIP、BIP及两条环引物LF、LB。在Bst DNA Polymerase作用下,60℃恒温水浴进... 详细信息

建立了环介导恒温扩增技术检测携带tdh基因的致病性副溶血性弧菌。基于副溶血性弧菌高度保守的tdh基因序列,设计了6条特异性引物,两条外引物F3、B3,两条内引物FIP、BIP及两条环引物LF、LB。在Bst DNA Polymerase作用下,60℃恒温水浴进行扩增。对10种细菌共21株菌进行LAMP扩增,所试6株副溶血性弧菌均为阳性,说明引物具有高度特异性。本LAMP方法对纯培养物的灵敏度可达到9.42cfu/m L。对污染食品中副溶血性弧菌的灵敏度为25.3cfu/25g,40～60min内即可完成检测。本方法操作简便、特异性强、灵敏度高,可以为临床提供简单、快速、高灵敏度和高特异性的检测应用。

关键词：副溶血性弧菌环介导恒温扩增 SYBR Green I 耐热溶血毒素(TDH) 快速检测

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牛蒡皮抑菌性能及其抑菌组分研究

食品工业 2014年第5期35卷 178-182页

作者：贺菊萍赵勇孙晓红 vivian chi-huawu 赵文秀潘迎捷上海海洋大学食品学院上海201306 徐州工程学院食品工程学院徐州221008 缅因大学食品科学与人类营养学系缅因04469-5735

比较研究了不同溶剂提取所得牛蒡皮的抑菌性能，对牛蒡皮中抑茵活性物质进行了分析。分别以氯仿、无水乙醇、丙酮、异丁醇、乙酸乙酯、石油醚提取牛蒡皮，滤纸片法研究了6种处理方法所得牛蒡提取物对大肠杆菌O157：H7，肠炎沙门氏菌，... 详细信息

比较研究了不同溶剂提取所得牛蒡皮的抑菌性能，对牛蒡皮中抑茵活性物质进行了分析。分别以氯仿、无水乙醇、丙酮、异丁醇、乙酸乙酯、石油醚提取牛蒡皮，滤纸片法研究了6种处理方法所得牛蒡提取物对大肠杆菌O157：H7，肠炎沙门氏菌，单增李斯特菌，金黄色葡萄球菌，副溶血性弧菌，恶臭假单胞菌、荧光假单胞菌、巨型葡萄球菌的抑菌效果。结果表明，6种提取物对供试菌种均有一定的抑茵作用，其中，乙酸乙酯和乙醇提取物抑菌效果最好。分别以石油醚、氯仿、乙酸乙酯、正丁醇对牛蒡皮提取物进行萃取，对各部分抑菌活性进行分析。石油醚相抑茵活性显著（p〈0．05）优于其他萃取相。采用硅胶层析柱对石油醚相进行分离纯化得到9个级分S1～S9，9个级分均具有抑菌活性，从S5中分离得化合物X经质谱与核磁共振鉴定为豆甾醇和 β-谷甾醇的混合物。

关键词：牛蒡皮抑菌分离纯化豆甾醇 β-谷甾醇

REP-PCR及ERIC-PCR法对分离自海产品副溶血性弧菌分型分析

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食品科学 2013年第10期34卷 263-267页

作者：马月姣孙晓红赵勇卢瑛吴启华潘迎捷上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心上海201306 缅因大学食品科学与人类营养系美国欧洛诺04469

目的:采用细菌基因外重复回文序列扩增(REP-PCR)和肠道细菌基因间重复序列扩增(ERIC-PCR)两种方法对副溶血性弧菌2株标准株,13株实验室保存菌株和73株分离菌株共88株进行分子分型。方法:通过REP和ERIC-PCR指纹图谱扩增,利用NTsys-pc软... 详细信息

目的:采用细菌基因外重复回文序列扩增(REP-PCR)和肠道细菌基因间重复序列扩增(ERIC-PCR)两种方法对副溶血性弧菌2株标准株,13株实验室保存菌株和73株分离菌株共88株进行分子分型。方法:通过REP和ERIC-PCR指纹图谱扩增,利用NTsys-pc软件采用Dice系数对指纹图谱进行聚类结果分析。结果:所有供试菌株可通过此两种方法进行分型得到清晰的指纹图谱,并反映出副溶血性弧菌具有丰富的遗传多样性,REP-PCR扩增出5～9条分布在609～4200bp之间的条带,可将副溶血性弧菌分为5个群12类型,分辨指数达0.93,其中tdh+株在相似系数0.86时可聚类在第Ⅰ群;ERIC-PCR扩增出5～11条分布在400～7593bp之间的条带,将副溶血性弧菌分为7个群11个类型,分辨指数为0.94,其中tdh+株在相似系数0.76时可聚类在第Ⅰ群。结论:两种方法均显现出很好的分型能力,能够很好地将环境分离tdh+菌株和标准菌株聚类在一起,其中REP-PCR较ERIC-PCR重复性更好。

关键词：副溶血性弧菌 tdh 肠内细菌基因组间重复序列分析细菌基因外重复回文序列扩增分型

不同产区18种蓝莓提取物抗氧化与抑菌作用研究

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食品工业科技 2015年第1期36卷 119-123页

作者：姜文洁孙晓红朱颖杨晗刘晓钰吴启华潘迎捷上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心上海201306 美国缅因大学食品与农业学院缅因04469

本文以18种栽培蓝莓的乙醇提取物为研究对象,利用体外法研究了不同产区蓝莓提取物对DPPH自由基和羟基自由基的清除作用及还原能力,分别采用福林酚法、亚硝酸钠法和p H示差法测定了18种蓝莓中总酚、黄酮及花青素的含量,同时利用试管二倍... 详细信息

本文以18种栽培蓝莓的乙醇提取物为研究对象,利用体外法研究了不同产区蓝莓提取物对DPPH自由基和羟基自由基的清除作用及还原能力,分别采用福林酚法、亚硝酸钠法和p H示差法测定了18种蓝莓中总酚、黄酮及花青素的含量,同时利用试管二倍稀释法测定了不同产区18种蓝莓提取物对副溶血性弧菌的抑制作用。结果表明,不同产区18种蓝莓对DPPH自由基及羟基自由基均具有良好的清除作用,对DPPH自由基清除率的范围为11%~65%,对羟基自由基清除率的范围为20%~64%,杭州产区的蓝莓还原能力强于上海及青岛两个产区;不同产区18种蓝莓的总酚、黄酮及花青素含量不同,总酚含量较高的蓝莓具有较好的抗氧化能力;不同产区18种蓝莓粗提液对副溶血性弧菌表现出不同程度的抑制作用,其MIC值最小的为15.625mg/m L,最大的为250mg/m L。由此可以得出,不同产区不同品种蓝莓在功能和活性成分上存在差异。

关键词：不同产区蓝莓提取物抗氧化抑菌作用

The potential of berries to serve as selective inhibitors of pathogens and promoters of beneficial microorganisms

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Food Quality and Safety 2017年第1期1卷 3-12页

作者： Alison Lacombe vivian C.H.wu School of Graduate Studies and Research National University of Natural MedicinePortlandOregon ProduceSafety and Microbiology Research Unit Western Regional Research CenterAgricultural Research ServiceUnitedStates Department of AgricultureAlbanyCaliforniaUSA

OBJECTIVES:Berries are distinct from other foods because of their unique compounds with bioprotective effects and antimicrobial/prebiotic *** new knowledge of how these unique phytochemicals differentially affect microbial communities,inhibit foodborne pathogens,and conserve beneficial species,the health claims associated with berries can be further *** review explores components of berries that have antimicrobial or prebiotic properties and incorporates new knowledge gained from both in vitro and in vivo ***:With the continued research efforts,antimicrobials and prebiotics derived from berries may provide an alternative to synthetic preservatives and antibiotics in addition to providing health benefits to *** could be applied to food products or as dietary interventions through elucidating which compounds have antimicrobial properties and how pH and nutrient condition impact their *** addition,these compounds can be added to foods with beneficial microorganisms with minimal impact on their probiotic viability.

关键词： berries probiotics pathogens

Evaluation of biological effects of three neodymium compounds on silkworm,Bombyx mori

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Journal of Rare Earths 2021年第10期39卷 1289-1299,I0005页

作者： vivian Andoh huaiyang Guan Lin Ma Weiguo Zhao Long Li Guohua wu College of Biotechnology Jiangsu University of Science and TechnologyZhenjiang 212018China The Sericultural Research Institute Chinese Academy of Agricultural SciencesZhenjiang 212018China

Neodymium(Nd)is one of the most famous members of rare earth elements *** the increasing global demand for Nd,it can inevitably enter the environment and affect the ecological environment through the food chain and other ***,it is very important to study the biological effects of *** this study,the biological effects of 3 kinds of Nd compounds,*** oxide(Nd_(2)O_(3)),neodymium chloride(NdCl3)and neodymium nitrate(Nd(NO_(3))_(3)),were evaluated by using silkworm models through the method of intravascular *** of the three Nd compounds was prepared into 6 groups at different concentrations(40,50,60,70,80,90μg/μL).The results show that within the certain concentration range,Nd compounds show great damage to the growth of silkworms since high death rate is observed:the death rate from Nd2 O3 group is floating between 30%and 80%,the one from NdCl3 group is between 17%and 33%and that from Nd(NO3)3 group is between 23%and 37%.It also reveals that Nd compounds have some negative effects on the body length and weight of ***,the histophysiological study shows that the three Nd compounds cause no obvious negative effects on the cell morphologies in midgut,fat body and silk *** XRD and FTIR spectra show that the secondary and the crystalline structures of silks are not destroyed,the TGA and DTG data exhibit that the thermal stability of silks is not damaged,and the tensile test shows that the mechanical properties of silks are not affected significantly by the intake of Nd *** general,the intake of Nd compounds has significant inhibitory effects on silkworm growth but little negative impact on silk structures or cell morphology,the study can provide useful toxicological data for the safe application of Nd compounds in various fields.

关键词： Nd compounds Bombyx mori Model animal Biological effects Rare earths

Can public report cards affect consumer’s private learning of quality? An evaluation of the impact of Florida hospital report cards on acute myocardial infarction patient admission patterns

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Health 2013年第12期5卷 2181-2189页

作者： vivian Y. wu Pai Liu Palo Alto Research Center Palo Alto USA Sol Price School of Public Policy Schaeffer Center for Health Policy and Economics University of Southern California Los Angeles USA

The empirical literature on hospital report cards typically assumes that report card cannot interact with consumer’s private learning. This study examines the impact of the implementation of FL hospital quality reporting system in late 2004 on hospital admission patterns using a pre-post difference-in-difference design. The estimation model allows for the possibility that report-card learning may interact with non-report-card learning. The study sample is comprised of all patients admitted to any FL hospital between 2000 and 2008 with a principal diagnosis of acute myocardial infarction (AMI). We find that hospital admission patterns for AMI patients did not respond to report card information. However, we find evidence consistent with the possibility that the implementation of a report card system may stimulate consumers (either patients or physicians) to seek higher quality hospitals through private information channels.

关键词： Hospital Report Cards Hospital Admission Pattern Private Learning