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small intestinal bacterial overgrowth in inactive Crohn's disease:Influence of thiopurine and biological treatment

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World Journal of Gastroenterology 2014年第38期20卷 13999-14003页

作者： Cristina sánchez-Montes vicente Ortiz Guillermo Bastida Ester Rodríguez maría Yago Belén Beltrán mariam Aguas marisa Iborra vicente Garrigues Julio Ponce pilar Nos Gastroenterology Unit Hospital Universitari i Politécnic La Fe

AIM: To investigate the influence of thiopurines and biological drugs on the presence of small intestinal bacterial overgrowth (sIBO) in patients with inactive Crohn’s disease (CD).

关键词： Crohn’ s disease Bacterial overgrowth Thiopurines Biologics Inflammatory bowel disease

Hepatitis B transmission by cell and tissue allografts:How safe is safe enough?

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World Journal of Gastroenterology 2014年第23期20卷 7434-7441页

作者： pilar solves vicente Mirabet Manuel Alvarez Blood Bank Hospital Universitari I Politècnic La FeBulevar Sur46026 ValenciaSpain

More than 2 million human tissue transplants(bone,tendon,cartilage,skin,cornea,amniotic membrane,stem cells,heart valve,blood vessel,etc.),are performed worldwide every *** and tissues are shared between countries which have different regulations and laboratory equipment and represent a risk of hepatitis B virus(HBV)transmission that has become a global safety *** the risk of transfusiontransmitted HBV infection from blood donations has been estimated,the rate of HBV transmission from donors to recipients of allografts is unknown and varies between different *** are various important ways of reducing the transmission risk,but donor screening and donor testing are still the main factors for preventing HBV *** detection is included in the routine screening tests for cell and tissue *** standard test for preventing transplant-transmitted hepatitis B is the hepatitis B surface *** implementation of methods involving nucleic acid amplification and the new generation of reactives to detect viral antibodies or antigens with an immunoassay,has increased the sensitivity and the specificity of the screening *** objective of our research was to review the literature and critically analyse the different steps for avoiding HBV transmission in cell and tissue donors,focusing on the screening tests performed.

关键词： Hepatitis B virus Tissue bank Tissue transplantation

Role of 9-Lipoxygenase and α-Dioxygenase Oxylipin Pathways as Modulators of Local and systemic Defense

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Molecular Plant 2012年第4期5卷 914-928页

作者： Jorge vicente Tomas Cascon Begonya Vicedo pilar Garcia-Agustin Mats Hamberg Carmen Castresana Department of Plant Molecular Genetics Centro National de Biotecnologia (CNB-CSIC) Darwin 3 28049 Madrid Spain Grupo de Bioquimica y Biotecnologia Vegetal Area de Fisiologfa Vegetal Departarnento de Ciencias Agrarias y del Medio Natural. ESTCE Universitat Jaume I Campus de Riu Sec. 12071 Castellon Spain Division of Physiological Chemistry II Department of Medical Biochemistry and Biophysics Karolinska Institutet S-171 77 Stockholm Sweden

Plant 9-1ipoxygenases （9-LOX） and α-dioxygenases （α-DOX） initiate the synthesis of oxylipins after bacterial infection. Here, the role of these enzymes in plants＇ defense was investigated using individual Arabidopsis thaliana Ioxl and doxl mutants and a double Ioxl doxl mutant. studies with Pseudomonas syringae pv. tomato （Pst） revealed the enhanced susceptibility of Ioxl to the virulent strain Pst DC3000 and the partial impairment of Ioxl and doxl mutants to activate systemic acquired resistance. Notably, both defects were enhanced in the Ioxl doxl plants as compared with individual mutants. We found that pre-treatment with 9-LOX- and α-DOX-generated oxylipins protected plant tissues against bacterial infection. The strongest effect in this respect was exerted by 9-ketooctadecatrienoic acid （9-KOT）, which is produced from linolenic acid by 9-LOX. Quantification of 9-KOT revealed its accumulation after bacterial infection. The levels were reduced in Ioxl and Ioxl doxl plants but strongly increased in the doxl mutant due to metabolic interaction of the two pathways. Transcriptional analyses indicated that 9-KOT pre-treatment modifies hormone homeostasis during bacterial infection. The nature of the changes detected suggested that 9-KOT interferes with the hormonal changes caused by bacterial effectors. This notion was substantiated by the finding that 9-KOT failed to reduce the growth of PstDC3OOOhrpA, a mutant compromised in effector secretion, and of the avirulent strain Pst DC3000 avrRpml. Further support for the action of the 9-LOX- and α-DOX-oxylipin pathways as modulators of hormone homeostasis was the ob- servation that Ioxl doxl seedlings are hypersensitive to the growth-inhibitory effect of ABA and showed enhanced ac- tivation of ABA-inducible marker genes as compared with wild-type plants.

关键词： oxylipins plant defense ABA Arabidopsis.

Comparison of different statistical approaches to evaluate morphometric sperm subpopulations in men

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Asian Journal of Andrology 2016年第6期18卷 819-823页

作者： Jesus L Yaniz sandra vicente-Fiel Caries soler pilar Recreo Teresa Carretero Araceli Bono Jose M Berne pilar santolaria TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain Department of Functional Biology and Physical Anthropology University of Valencia 46100 Burjassot Valencia Spain R+D Department Proiser R+D Scientific Park University of Valencia 46980 Paterna Valencia Spain TECNOGAM research group Environmental Sciences Institute (IUCA) Departament of Human Anatomy and Histology Faculty of Health and Sport Sciences of Huesca Plaza Universidad 3 22002 Huesca Spain TECNOGAM research group Environmental Sciences Institute (IUCA) Hospital General San Jorge de Huesca Area de Urologla Av. Martfnez de Velasco 36 22004 Huesca Spain

This study was designed to characterize morphometric sperm subpopulations in normozoospermic men by using different statistical methods and examining their suitability to classify correctly different sperm nuclear morphologies present in human ejaculates. Ejaculates from 21 normozoospermic men were collected for the study. After semen collection and analysis, samples were prepared for morphometric determination. At least 200 spermatozoa per sample were assessed for sperm morphometry by computer-assisted sperm morphometry analysis （CAsA-Morph） using fluorescence. Clustering and discriminant procedures were performed to identify sperm subpopulations from the morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three morphometric subpopulations （large-round 30.4%, small-round 46.6%, and large-elongated 22.9%）. In the second analysis, using discriminant methods, the classification was made independently of size and shape. Three morphological categories according to nuclear size （small 〈10.90 μm^2, intermediate 10.91-13.07 μm^2, and large 〉13.07 μm^2） and four categories were defined on 400 canonical cells （100 × 4） from 10 men according to sperm nuclear shape （oval, pyriform, round, and elongated）. Thereafter, the resulting classification functions were used to categorize 4200 spermatozoa from 21 men. Differences in the class distribution were observed among men from both clustering and discriminant procedures. It was concluded that the combination of CAsA-Morph fluorescence-based technology with multivariate cluster or discriminant analyses provides new information on the description of different morphometric sperm subpopulations in normal individuals, and that important variations in the distribution of morphometric sperm subpopulations may exist between men, with possible functional implications.

关键词： computer-assisted sperm morphometry analysis man sperm subpopulations

A comparative study of the morphometry of sperm head components in cattle, sheep, and pigs with a computer-assisted fluorescence method

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Asian Journal of Andrology 2016年第6期18卷 840-843页

作者： Jesus L Yaniz sara Capistros sandra vicente-Fiel Carlos O Hidalgo pilar santolaria TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences University of Zaragoza Huesca Spain Animal Genetics and Reproduction Area Regional Agrifood Research and Development Service (SERIDA) 33394 Deva Gijan Spain

The aim of this study was to compare the sperm nuclear and acrosomal morphometry of three species of domestic artiodactyls; cattle （Bos taurus）, sheep （Ovis aries）, and pigs （sus scrofa）. semen smears of twenty ejaculates from each species were fixed and labeled with a propidium iodide-Pisum sativum agglutinin （PI/PsA） combination. Digital images of the sperm nucleus, acrosome, and whole sperm head were captured and analyzed. The use of the PI/PsA combination and CAsA-Morph fluorescence-based method allowed the capture, morphometric analysis, and differentiation of most sperm nuclei, acrosomes and whole heads, and the assessment of acrosomal integrity with a high precision in the three species studied. For the size of the head and nuclear area, the relationship between the three species may be summarized as bull 〉 ram 〉 boar. However, for the other morphometric parameters （length, width, and perimeter）, there were differences in the relationships between species for sperm nuclei and whole sperm heads. Bull sperm acrosomes were clearly smaller than those in the other species studied and covered a smaller proportion of the sperm head. The acrosomal morphology, small in the bull, large and broad in the sheep, and large, long, and with a pronounced equatorial segment curve in the boar, was species-characteristic. It was concluded that there are clear variations in the size and shape of the sperm head components between the three species studied, the acrosome being the structure showing the most variability, allowing a clear distinction of the spermatozoa of each species.

关键词： artiodactyls computer-assisted sperm morphometry analysis fluorescence microscopy image analysis sperm morphometry

From liver to hormones:The endocrine consequences of cirrhosis

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World Journal of Gastroenterology 2024年第9期30卷 1073-1095页

作者： Juan Eduardo Quiroz-Aldave Elman Rolando Gamarra-Osorio maría del Carmen Durand-Vásquez Luciana del pilar Rafael-Robles Jhean Gabriel Gonzáles-Yovera maría Alejandra Quispe-Flores Luis Alberto Concepción-Urteaga Alejandro Román-González José Paz-Ibarra marcio José Concepción-Zavaleta Non-communicable Diseases Endocrinology Research LineHospital de Apoyo ChepénChepén 13871Peru Department of Endocrinology Hospital Víctor Lazarte EchegarayTrujillo 13006Peru Department of Endocrinology Clínica Javier PradoLima 15046Peru Department of Gastroenterology Hospital Nacional Guillermo Almenara IrigoyénLima 15018Peru Department of Endocrinology Hospital de Emergencias GrauLima 15001Peru Internal Medicine Hospital Regional Docente de TrujilloTrujillo 13011Peru Department of Endocrinology Hospital Universitario de San Vicente FundaciónMedellin 050010Colombia Internal Medicine Universidad de AntioquiaMedellín 050010Colombia school of Medicine Universidad Nacional Mayor de San MarcosLima 15081Peru Department of Endocrinology Hospital Nacional Edgardo Rebagliati MartinsLima 15072Peru Carrera de Medicina Humana Universidad Científica del SurLima 15067Peru

Hepatocrinology explores the intricate relationship between liver function and the endocrine *** liver diseases such as liver cirrhosis can cause endocrine disorders due to toxin accumulation and protein synthesis *** its importance,assessing endocrine issues in cirrhotic patients is frequently *** article provides a comprehensive review of the epidemiology,pathophysiology,diagnosis,and treatment of endocrine disturbances in liver *** review was conducted using the PubMed/Medline,EMBAsE,and scielo databases,encompassing 172 *** cirrhosis is associated with endocrine disturbances,including diabetes,hypoglycemia,sarcopenia,thyroid dysfunction,hypogonadotropic hypogonadism,bone disease,adrenal insufficiency,growth hormone dysfunction,and secondary *** optimal tools for diagnosing diabetes and detecting hypoglycemia are the oral glucose tolerance test and continuous glucose monitoring system,*** can be assessed through imaging and functional tests,while other endocrine disorders are evaluated using hormonal assays and imaging *** options include metformin,glucagon-like peptide-1 analogs,sodium-glucose co-transporter-2 inhibitors,and insulin,which are effective and safe for diabetes *** standards are followed for managing hypoglycemia,and hormone replacement therapy is often necessary for other endocrine *** transplantation can address some of these problems.

关键词： Diabetes mellitus Hypoglycemia Thyroid diseases Hypogonadism Metabolic bone diseases Liver cirrhosis

Computer-assisted sperm morphometry fluorescence-based analysis has potential to determine progeny sex

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Asian Journal of Andrology 2016年第6期18卷 858-862页

作者： pilar santolaria Alfredo Pauciullo Miguel A silvestre sandra vicente-Fiel Leyre Villanova Alain Pinton Juan Viruel Ester sales Jesus L Yaniz TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Animal Production and Food Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte SIN 22071 Huesca Spain Department of Agriculture Forest and Food Sciences University of Torino Largo Paolo Braccini 2 10095 Grugliasco TO Italy Department of Functional Biology and Physical Anthropology University of Valencia 469100 Burjassot Valencia Spain UMR 1388 INRA-ENVT INPT Animal Cytogenetis GenPhysE National veterinary school of Toulouse 23 Chemin des Capelles 31076 Toulouse Cedex 3 France Mediterranean Institute of marine and terrestrial Biodiversity and Ecology (IMBE) University of Aix Marseille Chemin de la Batterie des Lions 13007 Marseille France TECNOGAM research group Environmental Sciences Institute (IUCA) Department of Agricultural and Environmental Sciences Higher Polytechnic School University of Zaragoza Ctra. Cuarte S/N 22071 Huesca Spain

This study was designed to determine the ability of computer-assisted sperm morphometry analysis （CAsA-Morph） with fluorescence to discriminate between spermatozoa carrying different sex chromosomes from the nuclear morphometrics generated and different statistical procedures in the bovine species. The study was divided into two experiments. The first was to study the morphometric differences between X- and Y-chromosome-bearing spermatozoa （sX and sY, respectively）. spermatozoa from eight bulls were processed to assess simultaneously the sex chromosome by FIsH and sperm morphometry by fluorescence-based CAsA-Morph. sX cells were larger than sY cells on average （P 〈 0.001） although with important differences between bulls. A simultaneous evaluation of all the measured features by discriminant analysis revealed that nuclear area and average fluorescence intensity were the variables selected by stepwise discriminant function analysis as the best discriminators between sX and sY. In the second experiment, the sperm nuclear morphometric results from CAsA-Morph in nonsexed （mixed sX and sY） and sexed （sX） semen samples from four bulls were compared. FIsH allowed a successful classification of spermatozoa according to their sex chromosome content. X-sexed spermatozoa displayed a larger size and fluorescence intensity than nonsexed spermatozoa （P 〈 0.05）. We conclude that the CAsA-Morph fluorescence-based method has the potential to find differences between X- and Y-chromosome-bearing spermatozoa in bovine species although more studies are needed to increase the precision of sex determination by this technique.

关键词： bull computer-assisted sperm morphometry analysis sexing sperm morphometry

Efficacy of a strategy for Implementing Guidelines for the Control of Cardiovascular Risk in Primary Healthcare

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World Journal of Cardiovascular Diseases 2020年第3期10卷 117-130页

作者： maria Jesús Fernández-Luque Luis maría sánchez-Gómez vicente Pastor y Aldeguer Carmen suárez-Fernández Belén sierra-García soledad Mayayo-vicente marta Ruiz-López ángela Gallego-Arenas Rosa sánchez Alcalde pilar Loeches-Belinchón Javier López-González Francisco Rodríguez-salvanés Blanca Novella-Arribas Dirección General de Investigación Docencia y Documentación Consejería de Sanidad Comunidad de Madrid Madrid Spain Instituto de Investigación sanitaria del Hospital Universitario de La Princesa (IP) Madrid Spain Universidad Autónoma de Madrid Facultad de Medicina Madrid Spain Hospital Universitario de la Princesa SERMAS Consejería de Sanidad Comunidad de Madrid Madrid Spain Gerencia Asistencial de Atención Primaria D.G. de Coordinación de Asistencia Sanitaria SERMAS Consejería de Sanidad Comunidad de Madrid Madrid Spain Unidad de Evaluación de Tecnologías sanitarias de Madrid Procesos Integrados de Salud Consejería de Sanidad Madrid Spain

Background: A number of strategies exist for the implementation of clinical practice guides (CPGs). Aim: To assess the efficacy of implementing a cardiovascular risk CPG based on an educational method involving opinion leaders, and the habitual method of dissemination among primary healthcare teams. Design and setting: Controlled, blinded, community intervention trial randomised by clusters. Methods: 21 primary healthcare centres were randomly assigned to either the intervention arm (n = 11) or the control arm (n = 10). The study subjects were patients aged ≥45 years assigned to the centres. The overall impact of the intervention was measured as the difference between the increase in the proportion of patients whose medical records showed the recording of all the variables necessary to calculate cardiovascular risk in both arms. Analyses were performed with Generalized Lineal Model on an intention-to-treat basis. Results: 917 subjects were included at the beginning of the trial (437 in the intervention arm and 480 in the control arm). 826 subjects were included in the final evaluation (436 in the intervention group and 390 in the control arm). At the end of the trial, the recording of the variables necessary for the calculation of the cardiovascular risk in the intervention group had increased more than in the control group (difference between increases 7.49% (95% CI 4.62 - 10.35)) after adjusting for confounding variables. Conclusions: Compared to the habitual method of dissemination, the implementation of this CPG using an educational method involving opinion leaders, improved the recording of the variables needed to calculate patients’ cardiovascular risk.

关键词： Randomized Controlled Trial Cluster Analysis Clinical Practice Guidelines Primary Healthcare Risk Factors Cardiovascular Diseases

synthesis and structural Characterization of Monocrystalline α-V<sub>2sub>O<sub>5sub> Nanowires

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Materials sciences and Applications 2016年第9期7卷 484-495页

作者： maría Luisa Tafoya Ronquillo Patricia santiago Jacinto pilar Ovalle Luis Rendón Vázquez Elizabeth Chavira martínez Ernesto marinero vicente Garibay Instituto de Física UNAM Circuito de la Investigación s/n Ciudad Universitaria México D.F. México Instituto de Investigaciones en Materiales UNAM Circuito de la Investigación s/n Ciudad Universitaria México D.F. México Purdue University Purdue Mall West Lafayette IN USA Instituto Mexicano del Petróleo IMP México D.F. México

A stable one-dimensional system in an orthorhombic α-V2O5 nanowires monocrystalline structure was obtained by a solvothermal method from a polymorphic V2O5 structure. The starting material was firstly submitted to ac... 详细信息

A stable one-dimensional system in an orthorhombic α-Vsub>2sub>Osub>5sub> nanowires monocrystalline structure was obtained by a solvothermal method from a polymorphic Vsub>2sub>Osub>5sub> structure. The starting material was firstly submitted to acid hydrolysis in Hsub>2sub>Osub>2sub> followed by a solvothermal treatment. The outcome of this procedure, a metastable phase of the one-dimensional system Vsub>10sub>Osub>24sub>·12Hsub>2sub>O/Vsub>3sub>Osub>7sub>·Hsub>2sub>O, was subsequently reoxidized by controlled heating in an open air system. The final product was an orange crystalline solid mainly formed by monocrystalline nanowires of α-Vsub>2sub>Osub>5sub> having lengths of tens of micrometers and widths of about 75 nm with a preferential [200] growth direction. It was found that the pH value of the initial solution plays an important role in the formation of the crystalline phase in the final products. Characterization was performed by X-ray diffraction (XRD), scanning electron microscopy (sEM) and high-resolution transmission electron microscopy (HRTEM). This study offers an alternate route for the synthesis of vanadium oxides and related compounds.

关键词： Nanostructures Oxides Crystal Growth X-Ray Diffraction Crystal structure