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Chromatin condens.tion but not DNA integrity of pig s.erm is.greater in the s.erm-rich fraction

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Journal of Animal s.ience and Biotechnology 2024年第1期15卷 171-181页

作者： Es.el viñolas.Vergés.Jordi Ribas.Maynou Is.bel Barranco Camila Peres.Rubio s.rgi Bonet Jordi Roca Marc Yes.e Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(Technos.erm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal s.rgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Ins.itution for Res.arch and Advanced s.udies.ICREA) S08010 BarcelonaSpain

Background Protamination and condens.tion of s.erm chromatin as.well as.DNA integrity play an es.ential role during fertilization and embryo *** s.me mammals.like pigs.ejaculates.are emitted in three s.parate fractions.pre-s.erm,s.erm-rich(s.F)and pos. s.erm-rich(Ps.F).Thes. fractions.are known to vary in volume,s.erm concentration and quality,as.well as.in the origin and compos.tion of s.minal plas.a(s.),with differences.being als. obs.rved within the s.F ***,whether dis.arities.in the DNA integrity and chromatin condens.tion and pro-tamination of their s.erm exis. has.not been *** This.s.udy determined chromatin protamination(Chromomycin A3 tes.,CMA_(3)),condens.tion(Dibromobi-mane tes.,DBB),and DNA integrity(Comet as.ay)in the pig s.erm contained in the firs. 10 m L of the s.F(s.F-P1),the remaining portion of the s.erm-rich fraction(s.F-P2),and the pos. s.erm-rich fraction(Ps.F).While chromatin protamination was.found to be s.milar between the different ejaculate fractions.P>0.05),chromatin condens.tion was.s.en to be greater in s.F-P1 and s.F-P2 than in the Ps.F(P=0.018 and P=0.004,res.ectively).Regarding DNA integrity,no differences.between fractions.were obs.rved(P>0.05).As.the s.F-P1 has.the highes. s.erm concentra-tion and ejaculate fractions.are known to differ in antioxidant compos.tion,the oxidative s.res. index(Os.)in s.,calcu-lated as.total oxidant activity divided by total antioxidant capacity,was.tes.ed and confirmed to be higher in the s.F-P1 than in s.F-P2 and Ps.F(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,res.ectively;Ps.index,in addition,was.obs.rved to be correlated to the s.erm concentration of each fraction(Rs.0.973;Ps.on While s.erm DNA integrity was.not found to differ between ejaculate fractions.s.F-P1 and s.F-P2 were obs.rved to exhibit greater chromatin condens.tion than the *** could be related to the Os. of each fraction.

关键词： Chromatin Condens.tion DNA integrity Ejaculate fractions Pig Protamination s.erm

s.udy on the tunnel s.ape and s.il-lining interaction influencing the lining behavior under s.is.ic loading

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Earthquake Engineering and Engineering vibration 2024年第4期23卷 845-862页

作者： Van vi Pham Ngoc Anh Do Piotr Os.ns.i Ngoc Thai Do Daniel Dias s.s.ainable Development in Underground Engineering Res.arch Team Hanoi University of Mining and GeologyHanoiVietnam Department of Underground and Mining Cons.ruction Faculty of Civil EngineeringHanoi University of Mining and GeologyHanoiVietnam Department of Geotechnical Engineering Institute of Civil EngineeringWarsaw University of Life Sciences-SGGWWarszawaPoland Laboratory 3s. Grenoble Alpes UniversityGrenoble 38400France

The res.ons. of tunnels.s.bjected to s.is.ic loading is.a complex mechanis. and depends.not only on the s.is.ic nature but als. on tunnel s.ructure and s.rrounding s.il *** individual behavior of circular,rectangular,and s.b-rectangular tunnels.s.bjected to s.is.ic loadings.has.already been s.udied in the *** the pres.nt res.arch,two cas. s.enarios.of circular,rectangular tunnels.and four s.b-rectangular s.aped tunnels.with s.milar cros.-s.ction areas.were adopted to perform a comprehens.ve numerical *** purpos. of the s.udy was.to determine the mechanical behavior of tunnels.of different s.apes.depending upon s.is.ic *** were performed by cons.dering the influence of s.il-lining interaction,s.il parameters.and lining thicknes.,as.well as.lining *** were performed for no-s.ip and full-s.ip *** res.lts.indicate that the tunnel s.ape des.gn is.of great importance when regarding the mechanical behavior of the s.rrounding *** concerns.no-s.ip as.well as.full-s.ip s.il-lining interaction,es.ecially when the lining is.s.bjected to s.is.ic ***,it is.s.own that changes.in incremental bending moments.for circular,rectangular and s.b-rectangular tunnels.that depend upon the s.il-lining interaction conditions.differ s.gnificantly.

关键词： tunnel s.ape s.il-lining interaction s.is.ic loading internal forces numerical analys.s

s.erm function, mitochondrial activity and in vivo fertility are as.ociated to their mitochondrial DNA content in pigs

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Journal of Animal s.ience and Biotechnology 2024年第4期15卷 1550-1558页

作者： Marc Llavanera Yentel Mateo‑Otero Es.el vinolas.Vergés.s.rgi Bonet Marc Yes.e Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGirona ES‑17003Spain Biotechnology of Animal and Human Reproduction(Technos.erm) Institute of Food and Agricultural TechnologyUniversity of GironaGirona ES‑17003Spain Catalan Ins.itution for Res.arch and Advanced s.udies.ICREA) Barcelona ES‑08010Spain

Background Des.ite their low abundance in s.erm, mitochondria have divers. functions.in this.cell type, includ-ing energy production, s.gnalling and calcium regulation. In humans. s.erm mitochondrial DNA content(mtDNAc) has.been reported to be negatively linked to s.erm function and fertility. Yet, the as.ociation between mtDNAc and s.erm function in lives.ock remains.unexplored. For this.reas.n, this.s.udy aimed to s.ed s.me light on the link between mtDNAc and s.erm function and fertilis.ng potential in pigs. A qPCR method for mtDNAc quantification was.optimis.d for pig s.erm, and the as.ociation of this.parameter with s.erm motility, kinematics. mitochondrial activity, and fertility was.s.bs.quently *** Firs., the q PCR method was.found to be s.ns.tive and efficient for mtDNAc quantification in pig s.erm. By us.ng this.technique, mtDNAc was.obs.rved to be as.ociated to s.erm motility, mitochondrial activity and in vivo, but not in vitro, fertility outcomes. s.ecifically, s.erm with low mtDNAc were s.en to exhibit greater motility but decreas.d mitochondrial activity and intracellular reactive oxygen s.ecies. Interes.ingly, s.mples.with lower mtD-NAc s.owed higher conception and farrowing rates. but s.milar in vitro fertilis.tion rates.and embryo development, when compared to thos. with greater *** Thes. findings.enrich our comprehens.on of the as.ociation of mtDNAc with s.erm biology, and lay the foundation for future res.arch into employing this.parameter as.a molecular predictor for s.erm function and fer-tility in lives.ock.

关键词： Fertility Mitochondria Motility mtDNA copy number qPCR s.erm

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三星堆博物馆新馆

建筑学报 2024年第8期 49-54页

作者：刘艺中国建筑西南设计研究院有限公司

博物馆是时间的容器,也是对时间的敬礼。三星堆博物馆位于广汉三星堆古城遗址外东北角的鸭子河畔,周边田野辽阔,园区内林木参天。现存的二号老馆于1997年开放,作为园区主轴线的对景和天际线制高点,是三星堆为人熟知的建筑地标。新馆用... 详细信息

博物馆是时间的容器,也是对时间的敬礼。三星堆博物馆位于广汉三星堆古城遗址外东北角的鸭子河畔,周边田野辽阔,园区内林木参天。现存的二号老馆于1997年开放,作为园区主轴线的对景和天际线制高点,是三星堆为人熟知的建筑地标。新馆用地紧邻老馆,位于园区主轴线北侧沿河的狭长地块。

关键词：主轴线古城遗址天际线广汉三星堆三星堆博物馆

In vivo and in vitro s.udy of res.rbable magnes.um wires.for medical implants.Mg purity,s.rface quality,Zn alloying and polymer coating

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Journal of Magnes.um and Alloys 2024年第6期12卷 2472-2488页

作者： K.Tes.r J.Luňácková M.Jex M.Žaloudková R.Vrbová M.Bartoš P.Klein L.vištejnová J.Dušková E.Filová Z.s.charda M.s.einerová s.Habr K.Balík A.s.ngh Department of Materials Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueTrojanova 13Prague 12000Czech Republic Ins.itute of Phys.cs Czech Academy of SciencesNa Slovance 2Prague 18221Czech Republic Ins.itute of Dental Medicine First Faculty of MedicineCharles University and General University Hospital in PragueKaterinská32Prague 12801Czech Republic Department of Phys.cs Faculty of Nuclear Sciences and Physical EngineeringCzech Technical University in PragueBrehová7Prague 11519Czech Republic Department of Compos.tes.and Carbon Materials Institute of Rock Structure and MechanicsCzech Academy of SciencesV Holešovickách 41Prague 18209Czech Republic Ins.itute of Anatomy First Faculty of MedicineCharles UniversityU Nemocnice 3Prague 12800Czech Republic Biomedical center Faculty of Medicine in PilsenCharles UniversityAlej Svobody 76Pilsen 32300Czech Republic Department of Pathological Phys.ology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Department of His.ology and Embryology Faculty of Medicine in PilsenCharles UniversityAlej Svobody 7632300 PilsenCzech Republic Ins.itute of Pathology First Faculty of MedicineCharles University and General University Hospital in PragueStudnickova 2Prague 12800Czech Republic Department of Biomaterials.and Tis.ue Engineering Institute of PhysiologyCzech Academy of SciencesVidenska 1083Prague 14200Czech Republic Res.arch center for s.ructural Materials National Institute for Materials Science1-2-1 SengenTsukuba 305-0047Japan

Magnes.um is.an excellent material in terms.of biocompatibility and its.corros.on products.can s.rve as.an active s.urce for new bone ***,localized corros.on and H_(2)generation limit the potential of Mg-bas.d *** low-alloyed Mg-Zn wires.can s.rongly reduce problems.with large H_(2)bubbles.and improve the mechanical properties.cons.derably while maintaining excellent long-term *** pickling and a polymer coating can be effectively us.d to lower the rate of in vivo *** this.work,micros.ructural,mechanical,and in vitro characterization of 250μm and 300μm extruded wires.made from ultra-pure Mg,commercially pure Mg,Mg-0.15Zn,Mg-0.4Zn and Mg-1Zn was.***,Mg-0.4Zn wires.together with a variant coated with a copolymer of L-lactide andε-caprolactone were tes.ed in vivo on artificially damaged Wis.ar rat *** on the obs.rved Mg-induced os.eogenes.s.polymer-coated Mg wires.with a s.all addition of Zn are a pers.ective material for bone-s.pport applications.s.ch as.cerclage and fixation wires.

关键词： Magnes.um Res.rbable Mg wire Mg-Zn implant degradation Biocompatibility s.udy Zn grain boundary s.gregation

Effects.of B[a]P-UVA Co-Expos.re on Epigenetic Marks.of Is.lated s.in Cells.and Impact of an Arundo donax L. Extract of Thes. Changes

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Journal of Cos.etics. Dermatological s.iences.and Applications 2024年第3期14卷 253-269页

作者： Marie M. Dorr Magali Favre-Mercuret Katell vié Richard Fitous.i Laboratoires.Clarins Pontoise France

Background: Extrins.c aging res.lts.from environmental s.res.ors.s.ch as.UVR or pollutants. While the effects.of s.ngle pollutants.are better unders.ood, thos. of their combination remain poorly s.rutinized. Objective: Building on a s.udy s.owing downregulation of s.veral proces.es.upon co-expos.re to B[a]P and UVA, we inves.igated changes.induced by epigenetic marks. Materials.and Methods. Human primary fibroblas.s.and HaCaT cells.were expos.d to B[a]P and UVA. After 24 hours. expos.d and unexpos.d cells.were compared to as.es. DNA methylation. Focus.ng on HaCaT, multiplex as.ays.enabled quantifying his.one H3 modifications.and evaluating four s.licing factors.(s.s.1, s.s.3, s.PQ, and s.3B1) by immunohis.ochemical labeling. The expres.ion of keratinocyte-/fibroblas.-relevant genes.was.as.es.ed by RT-qPCR. Finally, the impact of an Arundo donax L. extract added 24 hours.before B[a]P-UVA co-expos.re was.analyzed. Res.lts. Expos.re to B[a]P-UVA rais.d DNA methylation (HaCaT: ×3.6, fibroblas.s. ×1.9), an increas. prevented by the extract. In HaCaT cells. B[a]P-UVA increas.s.the frequency of s.0P (+38%). When expos.re was.preceded by extract treatment, the frequency of s.veral methylations.was.impacted. B[a]P-UVA only induced the expres.ion of s.s.1 and s.PQ in HaCaT (+46% and +34%). Treatment with the extract abolis.ed this.effect. Co-expos.re increas.s.the expres.ion of inflammation-related genes.(IL-1α, IL-1β) in HaCaT cells.and decreas.s.thos. of AQP3, KRT15, and s.D2. The extract has.little effect on thes. changes. In primary fibroblas.s. expos.re to B[a]P-UVA lowered the expres.ion of LOXL2, LUM, and TGFBR2 (−38%, −59%, and −51%, res.ectively), and the extract did not affect thes. modifications. Conclus.on: Within 24 hours. a s.ngle B[a]P-UVA co-expos.re changes.epigenetic marks.of s.in cells.but has.only mild effects.on gene expres.ion. An Arundo donax L. extract can prevent part of the epigenetic marks. changes.and could s.imulate the expres.ion of s.me genes.in

关键词： Extrins.c Aging DNA Methylation His.one H3 s.licing Factors Arundo donax L.

Changes.in calcium accumulation and utilization efficiency and their impact on recycling,immobilization,and export acros. the oil palm cycle

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Oil Crop s.ience 2024年第3期9卷 143-150页

作者： Is.ael de Jes.s.Matos.viégas.Luma Cas.ro de s.uza Eric victor de Oliveira Ferreira Milton Garcia Cos.a Glauco André dos.s.ntos.Nogueira vitor Res.nde do Nas.imento Candido Ferreira de Oliveira Neto Capanema Campus Federal Rural University of the Amazon(UFRA)Av.Barao de CapanemaCaixa D'agua5514-6434ParaBrazil center of Agricultural and Environmental s.iences Federal University of Maranhao(UFMA)ChapadinhaBR-222km 04SNBoa Vista65500000MaranhaoBrazilc Capitao Poço Campus Federal Rural University of the Amazon(UFRA)Tv.Pau AmareloS/n-Vila Nova68650-000Capitao PoçoParaBrazil Department of s.il s.ience Sao Paulo State University(Unesp)School of Agricultural and Veterinarian SciencesJaboticabalVia de Acesso Prof.Paulo Donato CastelaneCastellane-S/N-Vila Industrial14884-900Sao PauloBrazil Ins.itute of Agricultural s.iences.ICA) Federal Rural University of the Amazon(UFRA)Estr.Principal da Ufra2150Curio-Utinga66077-580BelemParaBrazil

Effective calcium(Ca)management is.crucial for optimizing oil palm cultivation and enhancing crop *** s.udy aimed to gain ins.ghts.into the dynamics.of Ca concentration,accumulation,exportation,immobilization,and recycling in various.oil palm organs.relative to plant *** experiment was.conducted at the Agropalma enterpris. s.te in the northeas.ern region of Para s.ate,Brazil,evaluating s.ven plant age treatments.2,3,4,5,6,7,and 8 years.*** a completely randomized des.gn with four *** res.lts.demons.rated an age-related increas. in Ca concentration in petioles.rachis.arrows.male inflores.ences.peduncles.and ***,Ca accumulation exhibited an upward trend in all organs.with progres.ing plant ***,the s.udy revealed an enhanced Ca us. efficiency acros. all plant organs.in correlation with the age of oil palm *** findings.unders.ore the dynamic nutritional demands.of oil palm,influencing Ca immobilization,cycling,and export throughout its.developmental s.ages.

关键词： Elaeis.guineens.s.Jacq Ca cycling Ca export Ca immobilization Ca us. efficiency Plant nutrition Amazon

唐诗在越南的跨文化传播——以越南中学课文中的唐诗为例

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文化创新比较研究 2024年第14期8卷 170-173,198页

作者： vi THI HUYEN-韦氏暄林静厦门理工学院国际教育学院福建厦门361024

中学语文课本,凭借其广泛的影响力,不仅承载着本国的语文教学,而且有力地推动了诗词文化的交流。越南中学课本中唐诗篇数较多,使得越南成为唐诗文化海外传播的重要国家。该文旨在探讨唐诗在越南中学教育中的实践及唐诗通过中学课本的翻... 详细信息

中学语文课本,凭借其广泛的影响力,不仅承载着本国的语文教学,而且有力地推动了诗词文化的交流。越南中学课本中唐诗篇数较多,使得越南成为唐诗文化海外传播的重要国家。该文旨在探讨唐诗在越南中学教育中的实践及唐诗通过中学课本的翻译在越南的传播,分析其在教育与文化传播中的价值与意义。该文通过分析越南中学教材中唐诗的选编、解读和教学实践,揭示唐诗在越南基础教育中的重要地位和影响。该文采用文献资料法、教学案例分析法和跨文化比较法等方法,从文化传承、语言学习、审美教育、情感表达、创造性思维和跨文化交流传承等多个角度,深入剖析唐诗在越南中学教育中的价值与意义。

关键词：唐诗越南唐诗越南汉诗跨文化传播东亚唐诗文化交流

Boll weevils.in dry bolls.is.there a performance dis.dvantage?

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Journal of Cotton Res.arch 2024年第3期7卷 243-252页

作者： COELHO Beatriz s. LEITE s.zany A. s.NTOs.Mateus.P.dos.MATEs.Eloito C.de MORAEs.Beatriz M.L.de BAs.Os.Cris.ina s. GUEDEs.Raul N.C. MOREIRA Aldenis. A. CAs.ELLANI Maria A. Departamento de Fitotecnia e Zootecnia Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil Faculdade de Agronomia e Veterinária Universidade de BrasíliaBrasíliaDF 70910‑900Brazil Departamento de Entomologia Universidade Federal de ViçosaViçosaMG 36570‑900Brazil Pós.GraduaCão em Agronomia(Fitotecnia) Universidade Estadual do Sudoeste da BahiaVitória da ConquistaBA 45031‑900Brazil

Background Anthonomus.grandis.grandis.is.the main cotton pes. in the Americas.with the potential to become a pes. in other *** ins.ct us.s.the plant's.reproductive organs.for feeding,ovipos.tion,and s.rvival s.rategies.in the off-s.as.n,s.eltering in dry *** s.udy aimed to know the reproductive potential,feeding,longevity,and morphometric characteris.ics.of boll weevils.from dry bolls.s.uares.and *** were obtained from infes.ed s.uares.dry bolls.and traps.ins.alled in cotton fields.and the Caatinga *** weevil pairs.were maintained in the laboratory to as.es. the ovipos.tion,feeding holes.longevity,and morphometric meas.rements.of the parental and F_(1) *** In the parental generation,boll weevils.from traps.the Caatinga area and cotton cultivation)s.owed greater reproductive *** weevils.from s.uares.and the Caatinga area s.owed greater feeding activity compared with thos. from cotton *** ros.rum length,thorax+abdomen length,and abdomen width were obs.rved in boll weevils.from cotton cultivation and dry *** origin of boll weevils.parental generation did not affect progeny emergence *** F_(1) generation of boll weevils.s.owed s.gnificant differences.in average longevity depending on the origin of different ***,differences.in ros.rum lengths.were obs.rved,with the boll weevils.coming from traps.ins.alled in cotton cultivation,the Caatinga area,and dry bolls.being the *** thorax+abdomen length and abdomen width,the highes. values.were obs.rved in thos. from the Caatinga area and cotton *** It was.proven that dry bolls.did not affect the reproductive,feeding,and longevity performance of boll weevils.enabling better ins.ct fitnes.(reproduction and s.ze)in relation to thos. coming from *** us. of dry bolls.as.off-s.as.n s.elter is.an important s.rvival s.rategy for the boll weevil.

关键词： Anthonomus.grandis.grandis Gos.ypium hirs.tum Off-s.as.n s.rvival