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Effi ciency of phosphorus accumulation by plankton,periphyton developed on submerged artifi cial substrata and metaphyton:in-situ observation in two shallow ponds

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Journal of Oceanology and Limnology 2021年第3期39卷 928-945页

作者： Marija PEĆIĆ Slađana POPOVIĆ vesna MILUTINOVIĆ Gordana SUBAKOV SIMIĆ Ivana TRBOJEVIĆ Dragana PREDOJEVIĆ Institute of Botany and Botanical Garden‘Jevremovac’ Faculty of BiologyUniversity of BelgradeBelgrade 11000Serbia Institute of Chemistry Technology and MetallurgyScientific InstitutionNational InstituteCenter for Ecology and TechnoeconomicsUniversity of BelgradeBelgrade 11000Serbia Institute of Public Health of Belgrade Belgrade 11000Serbia

Phosphorus overenrichment of shallow ponds prevailing in wetlands leads to their eutrophication causing the collapse of those vulnerable *** potential of phosphorus accumulation by periphyton developed on artificial substrata has been investigated in two shallow ponds(Bara?ka and?iroki Rit)in northwest Serbia and compared to the same ability of plankton and *** periphyton substrate carrier has been submerged from May to *** continuously(CS)and monthly developed(MS)periphyton were *** component of all investigated communities has been qualitatively *** accumulation of only 14.7 mg TP/m^(2) was recorded in three-month exposed periphyton *** exposed from July to August reached maximal 12.7 mg TP/m^(2).Plankton community that was characterized by more diverse and abundantly developed algal component was more effective in phosphorus accumulation(0.7 mg/g dry weight)in comparison with dominantly inorganic and diatom-dominated periphyton in Bara?*** conditions caused by recent revitalization(dredging organic matter and mud from pond basin—redigging)as well as rapid desiccation of?iroki Rit disabled making an unambiguous conclusion about the efficiency of phosphorus accumulation among different communities,but suggested slight potential of phosphorus harvesting by metaphyton in this *** to the shorter exposure time that brings the reduced risk of unpredictable changes in the ecosystem,as well as the considerable amount of accumulated phosphorus,large-scale application of one-month exposed periphyton developed on artificial substrates would be more advisable for phosphorus harvesting in nutrient affected shallow ponds.

关键词： periphyton phosphorus accumulation shallow ponds (phyto)plankton metaphyton eutrophication

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Detection of gelatinase B activity in serum of gastric cancer patients

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World Journal of Gastroenterology 2006年第1期12卷 105-109页

作者： vesna V Dragutinovi Neboja S Radovanovi Lidija T Izrael-■ivkovi Miroslav M Vrvi Institute of Chemistry School of MedicineUniversity of BelgradeViegradska 2611 000 BelgradeSerbia and Montenegro Institute of Digestive Diseases Center of Esophagogastric SurgeryClinical Center of SerbiaKoste Todorovia 611 000 BelgradeSerbia and Montenegro Faculty of Chemistry University of BelgradeStudentski trg 1611001 BelgradeSerbia and Montenegro

AIM： To determine the proteolytic activity and expression of gelatinase B in serum of gastric cancer patients and their correlation with the stage of the tumor. METHODS： Sera from 23 patients who underwent surgery for primary gastric cancer as the experimental group and from 11 as the control group were used to determine the proteolytic activity and its inhibition by EDTA and 1,10-phenanthroline. Gelatinase B activity was detected by SDS polyacrylamide gel electrophoresis （SDS-PAGE） and SDS-PAGE zymography. RESULTS： Proteolytic enzyme activity was increased in gastric cancer patients when compared to the control group （,0〈0.05）. The proteinases were determined to be metalloproteinases upon inhibition test with specific metalloproteinase inhibitors 1,10-phenanthroline （P〈0.05） and EDTA （P〈0.01）. SDS-PAGE and SDS-PAGE zymography revealed gelatinase B （proMMP-9） activity and its molecular mass of 92 ku. CONCLUSION： Proteinase activity is overexpressed in serum of gastric cancer patients. Gelatinase B in serum plays an important role in the progression of gastric cancer. ProMMP-9 can be used as a marker for invasiveness of gastric *** activity; Inhibition

关键词： Matrix metalloproteinase-9 Gastric cancer

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Low-dose tenofovir is more potent than adefovir and is effective in controlling HBV viremia in chronic HBeAg-negative hepatitis B

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World Journal of Gastroenterology 2007年第30期13卷 4096-4099页

作者： Paolo Del Poggio Maurizio Zaccanelli Maria Oggionni Silvia Colombo Carlo Jamoletti vesna Puhalo Hepatology Unit Treviglio Hospital (Bg) Italy Blood Bank (Molecular Biology Section) Treviglio Hospital (Bg) Italy

AIM: To study the efficacy of tenofovir disoproxil fumarate (TDF) at low dose in a small open trial of chronic hepatitis B patients with advanced stage disease. METHODS: Eleven patients were treated with TDF 75 mg for a median period of 80 (range, 24-576) wk and then 7 cases were shifted to an adefovir 10 mg treatment group. All patients had been pre-treated with lamivudine: 5 had YMDD resistant mutants and 6 wild- type virus. When TDF was started, 4 patients had low- level viremia and 6 were PCR-negative. RESULTS: During TDF treatment, PCR remained negative in 10 patients, transaminase levels were normal and no significant viral breakthrough was observed. The drug was well tolerated in all cases. When TDF 75 mg was substituted with adefovir 10 mg, 3 out of 7 patients had a persistent viral rebound (2700-130 000 copies/mL), in whom lamivudine had to be reintroduced. CONCLUSION: Low-dose TDF monotherapy can control HBV viremia for an extended period of time without the emergence of resistance and is more potent than adefovir at the standard dosage. The use of a reduced dose of TDF could diminish the cost of therapy in low-income countries, but further studies in a larger population and in HBeAg-positive subjects are needed.

关键词： Tenofovir Chronic Hepatitis B Adefovir Nucleotide analogues Low-income countries

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Febrile cholestatic disease as an initial presentation of nodular lymphocyte-predominant Hodgkin lymphoma

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World Journal of Gastroenterology 2010年第35期16卷 4491-4493页

作者： Anna Mrzljak Slavko Gasparov Ika Kardum-Skelin vesna Colic-Cvrlje Slobodanka Ostojic Kolonic Slobodanka Ostojic-Kolonic University of ZagrebSchool of Medicine Department of Gastroenterology University Hospital Merkur Department of Pathology and Cytology University Hospital Merkur Laboratory for Cytology and Hematology Department of MedicineUniversity Hospital Merkur Department of Hematology University Hospital Merkur

Febrile cholestatic liver disease is an extremely unusual presentation of Hodgkin lymphoma(HL).The liver biopsy of a 40-year-old man with febrile episodes and cholestatic laboratory pattern disclosed an uncommon subtype of HL,a nodular lymphocyte-predominant HL(NLPHL).Liver involvement in the early stage of the usually indolent NLPHL's clinical course suggests an aggressiveness and unfavorable *** a liver biopsy early in the diagnostic algorithm enables accurate diagnosis and appropriate *** rare,HL should be considered in the differential diagnosis of cholestasis.

关键词： Cholestatic disease Hodgkin lymphoma Nodular lymphocyte-predominant Hodgkin lymphoma

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An unusual presentation of “silent” disseminated pancreatic neuroendocrine tumor

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World Journal of Gastroenterology 2004年第19期10卷 2919-2921页

作者： Dragomir Marisavljevic Natasa Petrovic Nikola Milinic vesna Cemerikic Miodrag Krstic Olivera Markovic Dragoljub Bilanovic Medical Center"Bezanijska kosa" BelgradeYugoslavia Clinical Center Serbia BelgradeYugoslavia

To present a patient diagnosed with pancreatic carcinoidthat was extremely rare and produced an atypical carcinoidsyndrome. We reported a 58-year old male patient whopresented with long standing, prominent cervicallymphadenopathy and occasional watery *** and immunohistochemical examinationof lymph node biopsy showed a metastatic neuroendocrinetumor, which was histological type A of carcinoid (EMA+,cytokeratin+, CEA-, NSE+, chromogranin A+, synaptophysin+,insulin-). Bone marrow biopsy showed identical *** site of the tumor was pancreas and diagnosis wasmade according to cytological and immunocytochemicalanalysis of the tumor cells obtained with aspiration biopsyof pancreatic mass (12 mm in diameter) under endoscopicultrasound guidance. However, serotonin levels in bloodand urine samples were normal. It is difficulty to establishthe precise diagnosis of a "functionally inactive" pancreaticcarcinoid and aspiration biopsy of pancreatic tumor underendoscopic ultrasound guidance can be used as a newpotent diagnostic tool.

关键词：基因表达播散性胰腺神经内分泌内分泌肿瘤腹泻

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GSTM1-null and GSTT1-active genotypes as risk determinants of primary open angle glaucoma among smokers

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International Journal of Ophthalmology(English edition) 2018年第9期11卷 1514-1520页

作者： Miroslav Stamenkovic vesna Lukic Sonja Suvakov Tatjana Simic Ivan Sencanic Marija Pljesa-Ercegovac vesna Jaksic Sinisa Babovic Marija Matic Aleksandra Radosavljevic Ana Savic-Radojevic Tatjana Djukic University Eye Clinic Medical Center ZvezdaraBelgrade 11000Serbia Faculty of Special Education and Rehabilitation University of BelgradeBelgrade 11000Serbia Institute of Medical and Clinical Biochemistry Belgrade 11000Serbia Faculty of Medicine University of BelgradeBelgrade 11000Serbia Hospital for Eye Diseases Clinical Center of SerbiaBelgrade 11000Serbia

AIM： To evaluate glutathione transferase theta 1 and mu 1（GSTT1 and GSTM1） polymorphisms as determinants of primary open angle glaucoma（POAG） risk, independently or in combination with cigarette smoking, hypertension and diabetes ***： A case-control study with 102 POAG patients and 202 age and gender-matched controls was carried out. Multiplex-polymerase chain reaction method was used for the analysis of GSTM1 and GSTT1 polymorphisms. The differences between two groups were tested by the t-test or χ~2 test. Logistic regression analysis was used for assessing the risk for disease ***： The presence of GSTM1-null genotype did not contribute independently towards the risk of POAG. However, individuals with GSTT1-active genotype were at almost two-fold increased risk to develop glaucoma（P=0.044） which increased up to 4.36 when combined with GSTM1-null carriers（P=0.024）. When glutathione transferase（GST） genotypes were analyzed in association with cigarette smoking, hypertension and diabetes, only carriers of GSTT1-active genotype had significantly increased risk of POAG development in comparison with GSTT1-null genotype individuals with no history of smoking, hypertension and diabetes, respectively （OR=3.52, P=0.003; OR=10.02, P〈0.001; OR=4.53, P=0.002）. CONCLUSION： The results obtained indicate that both GSTM1-null and GSTT1-active genotypes are associated with increased POAG risk among smokers, suggesting potential gene-environment interaction in glaucoma development.

关键词： GSTM1 GSTT1 primary open angle glaucoma risk smoking hypertension diabetes mellitus

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Treatment options in stage I seminoma

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Oncology Research 2022年第3期30卷 117-128页

作者： UROS BUMBASIREVIC MARKO ZIVKOVIC MILOS PETROVIC vesna CORIC NIKOLA LISICIC NEBOJSA BOJANIC Clinic of Urology University Clinical Center of SerbiaBelgrade11000Serbia Faculty of Medicine University of BelgradeBelgrade11000Serbia Institute of Medical and Clinical Biochemistry Faculty of MedicineUniversity of BelgradeBelgrade11000Serbia

Seminomas are most commonly diagnosed in clinical stage I(CSI).After orchiectomy,approximately 15%of patients in this stage have subclinical *** radiotherapy(ART)delivered to the retroperitoneum and ipsilateral pelvic lymph nodes has been the mainstay of treatment for many *** highly efﬁcient,with long-term cancer-speciﬁc survival(CSS)rates approaching almost 100%,ART is associated with considerable long-term consequences,particularly cardiovascular toxicity and increased risk of secondary malignancies(SMN).Therefore,active surveillance(AS)and adjuvant chemotherapy(ACT)were developed as alternative treatment *** AS prevents patient overtreatment,it is associated with strict follow-up regimens and increased radiation exposure due to repeated *** to equivalent CSS rates to ART,and lower toxicity,one course of adjuvant carboplatin presents the cornerstone of chemotherapy for CSI *** is almost 100%for patients with CSI seminoma,regardless of the chosen treatment ***,a personalized approach in treatment selection is ***,routine radiotherapy for CSI seminoma patients is no longer ***,it should be reserved for patients who are unﬁt or unwilling for AS or ***ﬁcation of prognostic factors for disease relapse allowed for the development of risk-adapted treatment strategy and stratiﬁcation of patients in low-risk and high-risk *** risk-adapted policy needs further validation,surveillance is currently recommended in low-risk patients,while ACT is reserved for patients with a higher risk of relapse.

关键词： Chemotherapy Radiotherapy Risk-adapted strategy Seminoma Surveillance

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Validation of the Glaucoma Quality of Life-15 Questionnaire in Serbian language

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International Journal of Ophthalmology(English edition) 2018年第10期11卷 1674-1684页

作者： Ivan Sencanic Tatjana Gazibara Jelena Dotlic Miroslav Stamenkovic vesna Jaksic Marija Bozic Anita Grgurevic Clinic for Eye Disease "Prof. dr Ivan Stankovic" University Medical Center "Zvezdara" Institute of Epidemiology Faculty of Medicine University of Belgrade Faculty of Medicine University of Belgrade Faculty of Special Education and Rehabilitation University of Belgrade Clinic for Eye Disease "Prof. dr Djordje Nesic" Clinical Center of Serbia

AIM：To translate the Glaucoma Quality of Life-15（GQL-15）to Serbian language and asses its validity and reliability in the population of Serbian ***：The study included 177 glaucoma *** parameters（visual acuity,mean defect and square root of loss variance of visual field）and socio-demographic data were *** were stratified according to the Nelson’s glaucoma staging system as mild,moderate and advanced *** patients filled out the GQL-15 and National Eye Institute Visual Function Questionnaire（NEI-VFQ 25）.The GQL-15 was translated following the internationally-accepted methodology,and its psychometric properties were assessed by using classical test theory and Rasch ***：The mean total score for the GQL-15 was 20.68±*** Cronbach’s alpha coefficient for the whole scale was 0.89（central and near vision,α=0.24;peripheral vision,α=0.85;glare and dark adaptation,α=0.83）.Factor analysis established 4 factors（70.3% of variance）：two corresponding to the original factors and two new factors specific for the Serbian *** GQL-15 score correlated positively with almost all clinical parameters and NEI-VFQ 25 proving good criterion *** of the GQL-15 total score on test-retest confirmed appropriate scale reproducibility（ρ=0.96,P〈0.001）.The GQL-15 discriminated well advanced from mild and moderate *** Rasch analysis we obtained adequate item（0.95）reliability *** all items had infit and outfit mean squares in the accepted ***：Serbian version of the GQL-15 demonstrates adequate reliability and *** version of the GQL-15is a valid instrument for evaluation of quality of life among Serbian speaking patients with glaucoma and can be applied in daily clinical work.

关键词： glaucoma GQL-15 scale validation Serbian

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Preparatory training attenuates drastic response of the insulin-like growth factor binding protein 1 at the point of maximal oxygen consumption in handball players

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Journal of Sport and Health Science 2017年第3期6卷 372-377页

作者： Olgica Nedic Milos Sunderic Goran Miljus Zoran Valdevit Vladimir Jakovljevic Marija Glibetic vesna Vucic Institute for the Application of Nuclear Energy University of BelgradeBelgrade 11080Serbia Faculty of Sport and Physical Education University of BelgradeBelgrade 11000Serbia Department of Physiology Faculty of Medical SciencesUniversity of KragujevacKragujevac 34000Serbia Centre of Research Excellence in Nutrition and Metabolism Institute for Medical ResearchUniversity of BelgradeBelgrade 11000Serbia

Background:Intensive exercise changes physiological need for glucose and several biochemical pathways responsible for its metabolism *** them are those which involve insulin,insulin-like growth factor(IGF-1),and IGF-binding proteins(IGFBPs).Different types and degrees of exercise,as well as an athlete's fitness,may induce a range of responses regarding concentrations and time needed for the *** idea of the work was to find out whether and how insulin/IGF axis responds to additional physical activity in the already trained subjects and if so,is the adaptation potentially beneficial from the aspect of metabolic ***:The effect of 4-week intensive training on campus(preparatory training) on the levels of insulin,IGF-1,and IGFBPs during maximal progressive exercise test(MPET) on a treadmill was compared to the results obtained during MPET conducted after a regular training season of a female elite handball team(n = 17,age:17 ± 1 years,height:171 ± 8 cm,weight:65 ± 8 kg,body mass index:22 ± 1 kg/m^2 at the beginning of the study;there were no significant changes at the end).Serum samples were obtained from players immediately before the test(basal),at the end of the test after reaching the point of maximal oxygen consumption(VO_(2max)),and after ***:The concentration of insulin decreased at VO_(2max),but remained higher in players after preparatory training(12.2 ± 2.5 m U/L vs.8.9 ± 4.4 m U/L,p = 0.049).The level of IGFBP-1 decreased in players at VO_(2max) in either case of training,but it remained much higher in tests performed after the preparatory regime than before(p = 0.029).Concentrations of IGF-1,IGFBP-2,-3,and-4 did not change ***:The inverse relation between insulin and IGFBP-1 was lost during MPET,as these 2 molecules changed in the same *** results obtained suggest less severe stress-induced depression of insulin and IGFBP-1 after preparatory *** another metabolic mechanism

关键词： Female IGF-binding proteins(IGFBPs) Insulin Insulin-like growth factor I(IGF-1) Progressive exercise test VO_(2max)

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On Some Topological Properties of Normed Boolean Algebras

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Advances in Pure Mathematics 2024年第5期14卷 367-387页

作者： vesna Gotovac Đogaš Faculty of Science University of Split Split Croatia

This paper concerns the compactness and separability properties of the normed Boolean algebras (N.B.A.) with respect to topology generated by a distance equal to the square root of a measure of symmetric difference between two elements. The motivation arises from studying random elements talking values in N.B.A. Those topological properties are important assumptions that enable us to avoid possible difficulties when generalising concepts of random variable convergence, the definition of conditional law and others. For each N.B.A., there exists a finite measure space ( E,ℰ,μ ) such that the N.B.A. is isomorphic to ( ℰ ˜ , μ ˜ ) resulting from the factorisation of initial σ-algebra by the ideal of negligible sets. We focus on topological properties ( ℰ ˜ , μ ˜ ) in general setting when μ can be an infinite measure. In case when μ is infinite, we also consider properties of ℰ ˜ fin ⊆ ℰ ˜ consisting of classes of measurable sets having finite measure. The compactness and separability of the N.B.A. are characterised using the newly defined terms of approximability and uniform approximability of the corresponding measure space. Finally, conditions on ( E,ℰ,μ ) are derived for separability and compactness of ℰ ˜ and ℰ ˜ fin .

关键词： Compact Locally Compact Polish Space Separable

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