GSTM1-null and GSTT1-active genotypes as risk determinants of primary open angle glaucoma among smokers

作     者：Miroslav Stamenkovic Vesna Lukic Sonja Suvakov Tatjana Simic Ivan Sencanic Marija Pljesa-Ercegovac Vesna Jaksic Sinisa Babovic Marija Matic Aleksandra Radosavljevic Ana Savic-Radojevic Tatjana Djukic 

作者机构：University Eye ClinicMedical Center ZvezdaraBelgrade 11000Serbia Faculty of Special Education and RehabilitationUniversity of BelgradeBelgrade 11000Serbia Institute of Medical and Clinical BiochemistryBelgrade 11000Serbia Faculty of MedicineUniversity of BelgradeBelgrade 11000Serbia Hospital for Eye DiseasesClinical Center of SerbiaBelgrade 11000Serbia 

出 版 物：《International Journal of Ophthalmology(English edition)》 (国际眼科杂志（英文版）)

年 卷 期：2018年第11卷第9期

页      面：1514-1520页

核心收录：

学科分类：1002[医学-临床医学] 100212[医学-眼科学] 10[医学] 

基　　金：Supported by the Serbian Ministry of Education,Science and Technological Development(No.175052 No.450009) 

主　　题：GSTM1 GSTT1 primary open angle glaucoma risk smoking hypertension diabetes mellitus 

摘      要：AIM： To evaluate glutathione transferase theta 1 and mu 1（GSTT1 and GSTM1） polymorphisms as determinants of primary open angle glaucoma（POAG） risk, independently or in combination with cigarette smoking, hypertension and diabetes ***： A case-control study with 102 POAG patients and 202 age and gender-matched controls was carried out. Multiplex-polymerase chain reaction method was used for the analysis of GSTM1 and GSTT1 polymorphisms. The differences between two groups were tested by the t-test or χ~2 test. Logistic regression analysis was used for assessing the risk for disease ***： The presence of GSTM1-null genotype did not contribute independently towards the risk of POAG. However, individuals with GSTT1-active genotype were at almost two-fold increased risk to develop glaucoma（P=0.044） which increased up to 4.36 when combined with GSTM1-null carriers（P=0.024）. When glutathione transferase（GST） genotypes were analyzed in association with cigarette smoking, hypertension and diabetes, only carriers of GSTT1-active genotype had significantly increased risk of POAG development in comparison with GSTT1-null genotype individuals with no history of smoking, hypertension and diabetes, respectively （OR=3.52, P=0.003; OR=10.02, P〈0.001; OR=4.53, P=0.002）. CONCLUSION： The results obtained indicate that both GSTM1-null and GSTT1-active genotypes are associated with increased POAG risk among smokers, suggesting potential gene-environment interaction in glaucoma development.