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Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves

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journal of Integrative Plant Biology 2018年第11期60卷 1028-1050页

作者： Ronghui Pan Sigrun Reumann Piotr lisik Stefanie Tietz laura j.Olsen jianping Hu MSU-Department of Energy Plant Research laboratory Michigan State UniversityEast LansingMI 48824USA Department of Molecular Cellularand Developmental BiologyUniversity of MichiganAnn ArborMIUSA Center of Organelle Research University of StavangerN-4021 StavangerNorway Department of Plant Biochemistry and Infection Biology Institute of Plant Science and MicrobiologyUniversity of HamburgD-22609 HamburgGermany Plant Biology Department Michigan State UniversityEast LansingMI 48824USA

Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide, metabolism of fatty acids, photorespiration, and the biosyn- thesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry （MS）-based proteomics has been per- formed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of perox- isomes from Arabidopsis leaves given a 48-h dark treatment. Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identified a total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the full function of plant peroxisomes.

关键词： Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves Figure YFP ATF

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Synthesis and Characterization of Carbon Conditioned with Iron Nanoparticles Using Pineapple-Peel

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Advances in Nanoparticles 2013年第4期2卷 384-390页

作者： G. García-Rosales l. C. longoria-Gándara S. Martínez-Gallegos j. González-juárez Posgrade Department Instituto Tecnologico de Toluca Metepec México Scientific Reseach and Reactor Instituto Nacional de Investigaciones Nucleares Ocoyoacac México

This paper presents the preparation of carbon conditioned with iron nanoparticles (CI) using a pineapple peel treated with iron salts, carboxymethylcellulose sodium and hexamine. First, the pineapple peel was analyzed by thermo gravimetric analysis (TGA) to determine the optimal temperature for pyrolysis. The formation of carbon conditioned by iron nanoparticles was studied as a function of time at 30 min, 60 min, 90 min, 120 min, 150 min and 180 min. Scanning electron microscopy (SEM) was used to identify changes in the morphology of the materials. The specific area of each material was obtained by the BET method. The elemental composition of pineapple-peel (PP), washed pineapple-peel (WPP) and carbon iron (CI), was determined by neutron activation analysis (NAA). The results show that the optimal time for obtaining spherical iron nanoparticles with a diameter between 10 nm and 30 nm is 180 min on the carbonaceous material with a specific surface area of 167 m2/g.

关键词： Pineapple Carbon Iron-Nanoparticles Synthesis Neutron Activation Analysis

Worsening Clinical Outcome with Increasing Number of So-Called Off-label or Unapproved Indications for Use of Drug Eluting Stents

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World journal of Cardiovascular Diseases 2016年第7期6卷 224-234页

作者： Anders M. Galløe Ulrik Abildgaard Henning Kelbæk Niels Bligaard jørgen l. jeppesen leif Thuesen Peter R. Hansen Department of Cardiology Zealand University Hospital Roskilde Denmark Department of Cardiology Copenhagen University Hospital Gentofte Denmark Department of Cardiology Copenhagen University Hospital Bispebjerg Denmark Department of Medicine Copenhagen University Hospital Glostrup Denmark Department of Cardiology Aalborg University Hospital Aalborg Denmark

Objective: We explored the impact of increasing number of off-label patient and lesion character-istics on 5-year clinical outcome following implantation of first generation drug eluting stents. Furthermore we tried to assess if stent performance might be improved if the off-label character-istics present were taken into account to guide the stent brand selection. Design: A total of 2888 coronary lesions treated with first generation drug eluting stents were classified for eventual presence of 18 different off-label characteristics and correlated to the 5-year clinical outcome by multiple stepwise Cox regression analysis. Results: The composite of cardiac death, myocardial infarction and target vessel revascularization (major adverse cardiac event, MACE) was 16.7% in cases with zero off-label characteristics and showed a stepwise increased rate up to 32.7% in cases with 5 off-label characteristics. The MACE rate for the sirolimus eluting stent appears to be 10% lower than the paclitaxel stent group and it may be further reduced by 10% - 18% if the off-label characteristics present in each case were taken into account to guide the stent brand selection. Conclusion: With increasing number of concomitantly appearing off-label characteristics there is a stepwise increase in MACE rate. The MACE rate may be reduced if the off-label characteristics were used to guide the stent brand selection.

关键词： Drug Eluting Stents

Effect of tuber sections and processing conditions on the physicochemical properties of sweet potato(Ipomoea batatas l.(lam))flour

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Food Quality and Safety 2019年第4期3卷 273-278页

作者： M.C.Iheagwara I.H.Chibuzo j.C.Ibeabuchi Department of Food Science and Technology Federal University of TechnologyOwerri P.M.B 1526 OwerriImo StateNigeria

Objective:The study was conducted to ascertain the effect of tuber sections,heat treatment,and rehydration with process chemicals of varying concentrations on the physicochemical properties of sweet potato(Ipomoea batatas l.(lam))*** and Methods:The flour samples were generated as raw,boiled,and steamed from the head,middle,and tail sections of the *** were compared with their whole tuber *** magnitude and extent of the factorial influence were measured using the effects on swelling index(SI),water absorption capacity(WAC),oil absorption capacity,total soluble solids(TSS),blue value index(BVI),gelling point temperature(GPT),boiling point temperature(BPT),and ***:The results obtained showed that the proximate composition from the tuber sections and the whole did not differ significantly(Pll the test parameter except for pH ***:From the investigation,it was observed that the test variables have significant effects on the physicochemical properties of the sweet potato flour.

关键词： sweet potato tuber sections physicochemical processing flour

Photocatalytic hydrogen production over metal-doped CdxZn1-xS under visible light

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Photocatalytic hydrogen production over metal-doped CdxZn1-x...

Ion acceleration and neutron production in hybrid gas-puff z-pinches on the GIT-12 and HAWK generators

第九届全国氢能学术会议

作者： G.j.liu,Z.H.Zhou,X.H.Zhang,D.W.jing,l.j.Guo Xi''an jiaotong University,China

＜正＞Photocatalytic water splitting using solar energy has been regarded as an attractive solution to resolve the global energy and environmental problems,but most of the photocatalysts that have been reported only work in ultraviolet region due to their wide band *** order to use the solar energy more effectively,visible-light-driven photocatalysts must be *** control the band structure,both of the methods which are doping of foreign elements into active photocatalysts with wide band gap and making solid solutions between photocatalysts with wide and narrow band gaps are often used.

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Matter and Radiation at Extremes 2020年第2期5卷 33-42页

作者： D.Klir S.l.j.ckson A.V.Shishlov V.A.Kokshenev K.Rezac A.R.Beresnyak R.K.Cherdizov j.Cikhardt B.Cikhardtova G.N.Dudkin j.T.Engelbrecht F.I.Fursov j.Krasa j.Kravarik P.Kubes N.E.Kurmaev V.Munzar N.A.Ratakhin K.Turek V.A.Varlachev Czech Technical University in Prague Faculty of Electrical Engineering16627 Prague 6Czech Republic Plasma Physics Division U.S.Naval Research LaboratoryWashingtonDistrict of Columbia 20375USA Institute of High Current Electronics SB RAS Tomsk634055Russia National Research Tomsk Polytechnic University Tomsk634050Russia Institute of Physics Academy of Sciences of Czech Republic18221 Prague 8Czech Republic Nuclear Physics Institute Academy of Sciences of Czech Republic18086 PragueCzech Republic

Z-pinch experiments with a hybrid configuration of a deuterium gas puff have been carried out on theHAWK(NRl,Washington,DC)and GIT-12(IHCE,Tomsk)pulsed power generators at 0.7 MA and 3MA currents,*** GIT-12,neutron yields reached an average value of 231012 neutrons,and deuterons were accelerated up to an energy of 30 *** was 50 times the ion energy provided by the generator driving voltage of 0.6 MV and the highest energy observed in z-pinches and dense plasma *** confirm these unique results independently on another device,we performed several experimental campaigns on *** of the experiments onGIT-12 andHAWKhelped us to understand which parameters are essential for optimized neutron *** theHAWKgenerator is of a similar pulsed power architecture as GIT-12,the experiments on GIT-12 and HAWK are important for the study of how charged-particle acceleration scales with the current.

关键词： acceleration generator independently

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有限差分偏移中的频散

石油物探译丛 1992年第1期 49-63页

作者： M.A.Brzostowski j.l.Black 田希泰

本文介绍一种时间域隐式有限差分偏移频散的实际度量方法,从而可以很容易计算频散随速度,倾角和采样参数(深度间隔、时间间隔和道间隔)的变化。本研究发现小采样间隔反而得不到好的结果,这是因为误差抵消不平衡造成的。另外一项结果是:... 详细信息

本文介绍一种时间域隐式有限差分偏移频散的实际度量方法,从而可以很容易计算频散随速度,倾角和采样参数(深度间隔、时间间隔和道间隔)的变化。本研究发现小采样间隔反而得不到好的结果,这是因为误差抵消不平衡造成的。另外一项结果是:可以使某一同相轴的误差保持平衡,但是不可能使所有同相轴的频散减到最小,因为地震剖面上通常会有各种不同的倾角和速度的缘故。我们还串级有限差分偏移频散计算方法,结果发现串级偏移并不能减小子波频散的幅度,然而它确实能使频散易于控制,因为可以避开多值速度存在时的参数选择问题。我们用模型和野外数据进行偏移试验以检验和证实理论频散度量。

关键词：地震勘探有限差分偏移频散量度

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Ruminal degradability of Guinea grass silage inoculated with Streptoccocus bovis isolated from bovine rumen combined or not with com wheat bran

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Agricultural Sciences 2013年第12期4卷 628-634页

作者： Emerson A. Bonelli Anderson de M. Zanine Alexandre l. de Souza Daniele de j. Ferreira Guilherme R. Alves Animal Science Department Universidade Federal de Mato Grosso Cuiabá Brasil

This experiment aimed to evaluate the ruminal degradability in situ of the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF) and acid detergent fiber (ADF) of Guinea grass silages. The experimental design was completely randomized with six treatments and six repetitions: Guinea grass silage, Guinea grass silage inoculated with 10% wheat bran, Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis jB1;Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis jB1 plus 10% of wheat bran;Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis HC5;Guinea grass silage inoculated with 106 cfu/g of strains of Streptococcus bovis HC5 plus 10% of wheat bran. The silages inoculated with Streptococcus bovis and with added wheat bran resulted in higher values of ruminal degradability of DM, 32.76% and 32.17%, and of crude protein 38.28% and 37.89%. For the effective and potential degradability of DM, CP, NDF and ADF the highest values occurred for silages in these silages as well, in all passage rates. There is a similarity between the responses of Streptococcus bovis jB1 and the HC5, and the wheat bran enhanced the effectiveness of the microbial additive.

关键词： Crude Protein Digestion Dry Matter Fiber Inoculant

施肥对11年生火炬松人工林菌根数量和林木生长的影响

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贵州林业科技 1983年第S1期 7-13页

作者： j.A.Menge l.F.Grand l.W.Haines 管国利

在11年生火炬松(Pinus taeda l.)人工林里进行的施肥研究中、我们发现除施N+P+K肥小区外,所有施N肥小区的菌根根尖数,都少于对照及只施p肥小区的菌根根尖数。由于单独施P肥对菌根根尖数量无影响,所以同时施N肥和P肥时,菌根根尖少,可认为... 详细信息

在11年生火炬松(Pinus taeda l.)人工林里进行的施肥研究中、我们发现除施N+P+K肥小区外,所有施N肥小区的菌根根尖数,都少于对照及只施p肥小区的菌根根尖数。由于单独施P肥对菌根根尖数量无影响,所以同时施N肥和P肥时,菌根根尖少,可认为N肥是其主要原因。

关键词：菌根根尖小区火炬松人工林林木生长