Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves

作     者：Ronghui Pan Sigrun Reumann Piotr Lisik Stefanie Tietz Laura J.Olsen Jianping Hu 

作者机构：MSU-Department of Energy Plant Research LaboratoryMichigan State UniversityEast LansingMI 48824USA Department of MolecularCellularand Developmental BiologyUniversity of MichiganAnn ArborMIUSA Center of Organelle ResearchUniversity of StavangerN-4021 StavangerNorway Department of Plant Biochemistry and Infection BiologyInstitute of Plant Science and MicrobiologyUniversity of HamburgD-22609 HamburgGermany Plant Biology DepartmentMichigan State UniversityEast LansingMI 48824USA 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2018年第60卷第11期

页      面：1028-1050页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by grants from the National Science Foundation to J.H.(MCB 0618335 MCB 1330441)and L.J.O.(MCB 0618279) 

主　　题：Proteome analysis of peroxisomes from dark-treated senescent Arabidopsis leaves Figure YFP ATF 

摘      要：Peroxisomes compartmentalize a dynamic suite of biochemical reactions and play a central role in plant metabolism, such as the degradation of hydrogen peroxide, metabolism of fatty acids, photorespiration, and the biosyn- thesis of plant hormones. Plant peroxisomes have been traditionally classified into three major subtypes, and in-depth mass spectrometry （MS）-based proteomics has been per- formed to explore the proteome of the two major subtypes present in green leaves and etiolated seedlings. Here, we carried out a comprehensive proteome analysis of perox- isomes from Arabidopsis leaves given a 48-h dark treatment. Our goal was to determine the proteome of the third major subtype of plant peroxisomes from senescent leaves, and further catalog the plant peroxisomal proteome. We identified a total of 111 peroxisomal proteins and verified the peroxisomal localization for six new proteins with potential roles in fatty acid metabolism and stress response by in vivo targeting analysis. Metabolic pathways compartmentalized in the three major subtypes of peroxisomes were also compared, which revealed a higher number of proteins involved in the detoxification of reactive oxygen species in peroxisomes from senescent leaves. Our study takes an important step towards mapping the full function of plant peroxisomes.