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Expression of α_(6) integrin subunit in bovine oocyte and its potential role during fertilization

Advances in Bioscience and Biotechnology 2013年第5期4卷 617-622页

作者： Roseli Fernandes Goncalves Ricardo Pimenta Bertolla Cassia Maria Barroso Orlandi valquiria hyppolito barnabe Department of Animal Reproduction College of Veterinary Medicine and Animal ScienceSao Paulo UniversitySao PauloBrazil Department of Surgery Division of UrologyHuman Reproduction SectionSao Paulo Federal UniversitySao PauloBrazil Laboratory of Animal Endocrinology College of Veterinary MedicineSao Paulo State UniversityAracatubaBrazil

Fertilization in mammals requires the successful completion of a sequence of steps, starting with the transport of gametes in the reproductive tract and ending with sperm-egg membrane fusion to produce a zygote. Although some integrin subunits are known to be associated with the plasma membrane of some mammalian oocytes and spermatozoa, the presence of α6 integrin on bovine oocytes with intact zona pellucida has not been reported. The present study was undertaken to evaluate the expression of α6 integrin subunit in bovine oocyte and to determine if in vitro binding to the zona pellucida and fertilization were affected by treating oocytes with α6 integrin subunit antibody. The α6 integrin subunit was identified on the bovine oocyte by immunocytochemistry. In vitro fertilization was significantly decreased when in vitro matured bovine oocytes were pre-incubated with α6 integrin subunit antibody at concentration 5 and 20 μg/mL, and spermoocyte binding increased. These studies demonstrated the presence of α6 integrin subunit on bovine oocyte, and its importance in fertilization and polyspermy.

关键词： Integrins Bovine Oocytes Fertilization Sperm-Oocyte Binding

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