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American Journal of Molecular Biology 2020年第3期10卷 246-258页

作者： Nadeem Sharif Muhammad Danish Mehmood Syed Zeeshan Haider Naqvi Huma Anwar ul-Haq Syed Shoaib ahmed Muhammad usman Ghani Muhammad Shoaib Mubashir Hussain Institute of Molecular Biology and Biotechnology University of Lahore Lahore Pakistan Ottoman Pharma (Immuno Division) Lahore Pakistan College of Veterinary Sciences FSH & VS Peshawar Pakistan Kohat University of Science & Technology Kohat Pakistan

Hydro-Pericardium Hepatitis (HPH) is an emerging infectious disease of commercial poultry, caused by different serotypes of Fowl Adeno Virus. The vertical transmission of the virus into the progeny may results in devastating damage, causing huge economic losses to its farmers. In present study, molecular typing is performed on basis of partially conserved hexon gene sequences, using a unique set of primers having common reverse oligo for simultaneous detection of FAdV1, FAdV-4 and FAdV-11. A total of 14 fowl adeno virus strains were isolated from 100 suspected adeno virus liver samples, collected from different districts in Pakistan, between 2018 and 2019. FASTA’s sequence alignment and phylogenetic analysis revealed that out of the 14, one isolate which belonged to group A showed 27% similarity with FAdV-1, while three isolates showed 99%, 95% & 45% similarity to FAdV-4 (Group C). Whereas, ten isolates showed more than 99% similarity to FAdV-11 (Group D). The serotypes FAdV1, FAdV-4 and FAdV-11 are prevailing in the breeder and broilers. These results hold great importance in rapid, reliable and simultaneous detection of the three FAdV serotypes. Therefore, fowl adeno virus vaccine production for commercial poultry shall be according to the prevalent field serotypes.

关键词： Hydro-Pericardium Hepatitis (HPH) Hexon Gene Polymerase Chain Reaction Phylogenetic Analysis Sanger’s Sequence

Countrywide Survey for MERS-Coronavirus Antibodies in Dromedaries and Humans in Pakistan

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Virologica Sinica 2018年第5期33卷 410-417页

作者： Ali Zohaib Muhammad Saqib Muhammad Ammar Athar Jing Chen Awais-ur-Rahman Sial Saeed Khan Zeeshan Taj Halima Sadia usman Tahir Muhammad Haleem Tayyab Muhammad Asif Qureshi Muhammad Khalid Mansoor Muhammad Ahsan Naeem Bing-Jie Hu Bilal ahmed Khan Ikram Din Ujjan Bei Li Wei Zhang Yun Luo Yan Zhu Cecilia Waruhiu Iahtasham Khan Xing-Lou Yang Muhammad Sohail Sajid Victor Max Corman Bing Yan Zheng-Li Shi CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China University of Ctunese Academy of Sciences Beijing 100049China Faculty of Veterinary Medicine University of AgricultureFaisalabad 38040Pakistan Laboratory of Cancer Biomarkers and Liquid Biopsy College of PharmacyHenan UniversityHenan 475004China Department of Clinical Studies Faculty of Veterinary and Animal SciencesPMAS-Arid Agriculture UniversityRawalpindi 46000Pakistan Dow University of Health Science Pakistan Karachi 74200 Department of Microbiology Government College University FaisalabadFaisalabad 38000Pakistan Livestock and Dairy Development Department(L&DD) Lahore 54000Pakistan College of Veterinary and Animal Sciences The Islamia University of BahawalpurBahawalpur 63100Pakistan State Key Laboratory of Agricultural Mlcroblology Huazhong Agricultural UniversityWuhan 430070China Liaqat University of Medical and Health Science Pakistan Jamshoro 76090 Section of Epidemiology and Public Health Department of Chnical SciencesUniversity of Veterinary and Animal SciencesLahore subcampusJhang 35200Pakistan Institute of Virology Chadte-Universitatsmedizin BerlinCorporate member of Free UniversityHumboldt-University and Berlin Institute of HealthBerlin 10117Germany German Center for Infection Research(DZIF) Berlin 10117Germany

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050)samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged [ 10 years (81.37%) followed by those aged 3.1–10 years (78.65%) and B 3 years (58.19%).Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016–2017. Among the sampled population, 840 humans were camel *** we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.

关键词： Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Camel -Human Pakistan

National guidelines for the diagnosis and treatment of hilar cholangiocarcinoma

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World Journal of Gastroenterology 2024年第9期30卷 1018-1042页

作者： Faisal Saud Dar Zaigham Abbas Irfan ahmed Muhammad Atique usman Iqbal Aujla Muhammad Azeemuddin Zeba Aziz Abu Bakar Hafeez Bhatti Tariq Ali Bangash Amna Subhan Butt Osama Tariq Butt Abdul Wahab Dogar Javed Iqbal Farooqi Faisal Hanif Jahanzaib Haider Siraj Haider Syed Mujahid Hassan Adnan Abdul Jabbar Aman Nawaz Khan Muhammad Shoaib Khan Muhammad Yasir Khan Amer Latif Nasir Hassan Luck Ahmad Karim Malik Kamran Rashid Sohail Rashid Mohammad Salih Abdullah Saeed Amjad Salamat Ghias-un-Nabi Tayyab Aasim Yusuf Haseeb Haider Zia Ammara Naveed Department of Hepatopancreatic Biliary Surgery&Liver Transplant Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Hepatogastroenterology and Liver Transplantation Dr.Ziauddin University HospitalKarachi 75600SindhPakistan University of Aberdeen Aberdeen B243FXUnited Kingdom Department of Pathology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Gastroenterology&Hepatology Pakistan Kidney and Liver Institute&Research CentreLahore 54000Pakistan Department of Radiology Aga Khan UniversityKarachi 74800Pakistan Department of Oncology Hameed Latif HospitalLahore 54000Pakistan Division of Hepatopancreatic Biliary Surgery&Liver Transplantation Shifa International HospitalIslamabad 44000Pakistan Department of Hepatopancreatic Biliary Surgery&Liver Transplant Shaikh Zayed Hospital and Post Graduate Medical InstituteLahore 54000Pakistan Department of Medicine Aga Khan University HospitalKarachi 74800Pakistan Department of Liver Transplant Pir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Medicine&Gastroenterology Lifecare Hospital and Research CentrePeshawar 25000Khyber PakhtunkhwaPakistan Department of Hepatopancreatobiliary&Liver Transplant Bahria International HospitalLahore 54000Pakistan Department of Surgery Hepatopancreatobiliary&Liver TransplantDow University of Health SciencesKarachi 74800Pakistan Department of Gastroenterology Hepatology&NutritionPir Abdul Qadir Shah Jeelani Institute of Medical SciencesGambat 66020Pakistan Department of Oncology Dr.Ziauddin HospitalKarachi 74800Pakistan Department of Radiology Rehman Medical InstitutePeshawar 25000Pakistan Army Liver Transplant Unit Pak Emirates Military HospitalRawalpindi 46000Pakistan Department of Gastroenterology Sindh Institute of Urology and TransplantationKarachi 75500Pakistan Rashid Nursing Home and Cancer Clinic Rashid Nursing Home and Cancer ClinicRawalpindi 46000Pakistan Department of Gastroentero

A consensus meeting of national experts from all major national hepatobiliary centres in the country was held on May 26,2023,at the Pakistan Kidney and Liver Institute&Research Centre(PKLI&RC)after initial consultations with the *** Pakistan Society for the Study of Liver Diseases(PSSLD)and PKLI&RC jointly organised this *** effort was based on a comprehensive literature review to establish national practice guidelines for hilar cholangiocarcinoma(hCCA).The consensus was that hCCA is a complex disease and requires a multidisciplinary team approach to best manage these *** coordinated effort can minimise delays and give patients a chance for curative treatment and effective *** diagnostic and staging workup includes high-quality computed tomography,magnetic resonance imaging,and magnetic resonance *** cytology or biopsy utilizing endoscopic retrograde cholangiopancreatography is a mainstay for ***,histopathologic confirmation is not always required before *** ultrasound with fine needle aspiration of regional lymph nodes and positron emission tomography scan are valuable adjuncts for *** only curative treatment is the surgical resection of the biliary tree based on the Bismuth-Corlette *** patients with unresectable hCCA can be considered for liver *** chemotherapy should be offered to patients with a high risk of *** use of preoperative biliary drainage and the need for portal vein embolisation should be based on local multidisciplinary *** with acute cholangitis can be drained with endoscopic or percutaneous biliary *** chemotherapy with cisplatin and gemcitabine has shown improved survival in patients with irresectable and recurrent hCCA.

关键词： Hilar cholangiocarcinoma Bismuth-Corlette classification Memorial Sloan Kettering Cancer Centre Staging Preoperative biliary drainage Portal vein embolisation Surgical resection Hepatectomy

Mapping of Soil Properties Using Geographical Information System (GIS): A Case Study of Hassan usman Katsina Polytechnic

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Open Journal of Civil Engineering 2018年第4期8卷 544-554页

作者： Jafar Adam Samaila Saleh Adekunle T. Olowosulu ahmed H. Ashara S. Srividhya Department of Civil Engineering Hassan Usman Katsina Polytechnic Katsina Nigeria Department of Civil Engineering Ahmadu Bello University Zaria Nigeria Department of Civil Engineering SRM University Chennai India

GIS relates and integrates spatial data from various sources, for storage, retrieval, analyses and display. This paper presents the analysis and mapping of Geotechnical data using natural neighbour interpolation tool of GIS. The Campus of Hassan usman Katsina Polytechnic Nigeria was as the study area, and Arc GIS 10.2.1 was used to perform the analyses. The study used soils samples from ten trial pit distributed within the study area and subjected to various laboratory tests. Index and engineering properties of the samples were determined. Map database developed in a GIS environment and the result of the laboratory tests stored, were analysed and displayed using GIS. The research results show maps of various soil properties. The research result provides estimated data for preliminary designing of engineering infrastructures in the area.

关键词： Geographic Information System Soil Properties and Hassan usman Katsina Polytechnic

The Pattern of Seminal Fluid Analysis among Male Partners Attending an Infertility Clinic in a Nigerian Tertiary Health Institution

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Open Journal of Obstetrics and Gynecology 2020年第7期10卷 957-967页

作者： Amina Gambo Umar Abubakar Abubakar Panti Marcus Mbakwe Yakubu ahmed Jamila Abubakar Garba Daniel Chukunyere Nnadi Department of Obstetrics and Gynaecology Usman Danfodiyo University Sokoto Nigeria Department of Obstetrics and Gynaecology Usman Danfodiyo University Teaching Hospital Sokoto Nigeria

Background: Semen analysis has remained an objective, inexpensive and readily available means of assessing male factor infertility. Aim and Objective: This study aims to determine the prevalence of male infertility, the types and semen quality among infertile couples attending infertility clinic at UDUTH, Sokoto. Materials and Method: This is a retrospective study carried out between January, 2012 to December, 2016. All case notes of infertile patients managed were retrieved The data obtained was analysed using SPSS version 20 and the results were presented in text, tables and charts. Results: Eight hundred and seventy-six out of 11,938 total gynaecological consultations over the study period were due to infertility, giving a prevalence of 7.3%. Among them, 34.4% (320) accounted for male factor infertility. The age ranged between 22 to 75 years with a mean of 37.44 years ± 7.44. Majority, 50.2% were within the ages of 35 - 44 years and up to 73.8% of them had at least secondary education and were mostly, 39.7% civil servants. Up to 75.4% had sperm abnormalities, among whic

关键词： Seminal Fluid Analysis Oligospermia Infertility Clinic Oligoasthenoteratozospermia

JUNO sensitivity on proton decay p→νK^(+)searches

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Chinese Physics C 2023年第11期47卷 7-22页

作者： Angel Abusleme Thomas Adam Shakeel Ahmad Rizwan ahmed Sebastiano Aiello Muhammad Akram 安丰鹏安琪 Giuseppe Andronico Nikolay Anfimov Vito Antonelli Tatiana Antoshkina Burin Asavapibhop João Pedro Athayde Marcondes de André Didier Auguste Nikita Balashov Wander Baldini Andrea Barresi Davide Basilico Eric Baussan Marco Bellato Antonio Bergnoli Thilo Birkenfeld Sylvie Blin David Blum Simon Blyth Anastasia Bolshakova Mathieu Bongrand Clément Bordereau Dominique Breton Augusto Brigatti Riccardo Brugnera Riccardo Bruno Antonio Budano Mario Buscemi Jose Busto Ilya Butorov Anatael Cabrera Barbara Caccianiga 蔡浩蔡啸蔡严克蔡志岩 Riccardo Callegari Antonio Cammi Agustin Campeny 曹传亚曹国富曹俊 Rossella Caruso Cédric Cerna 常劲帆 Yun Chang 陈平平 Po-An Chen 陈少敏陈旭荣 Yi-Wen Chen 陈义学陈羽陈长程捷程雅苹 Alexey Chetverikov Davide Chiesa Pietro Chimenti Artem Chukanov Gérard Claverie Catia Clementi Barbara Clerbaux Selma Conforti Di Lorenzo Daniele Corti Flavio Dal Corso Olivia Dalager Christophe De La Taille 邓智邓子艳 Wilfried Depnering Marco Diaz Xuefeng Ding 丁雅韵 Bayu Dirgantara Sergey Dmitrievsky Tadeas Dohnal Dmitry Dolzhikov Georgy Donchenko 董建蒙 Evgeny Doroshkevich Marcos Dracos Frédéric Druillole 杜然杜书先 Stefano Dusini Martin Dvorak Timo Enqvist Heike Enzmann Andrea Fabbri Ulrike Fahrendholz 范东华樊磊方建方文兴 Marco Fargetta Dmitry Fedoseev Li-Cheng Feng 冯启春 Richard Ford Amélie Fournier 甘浩男 Feng Gao Alberto Garfagnini Arsenii Gavrikov Marco Giammarchi Agnese Giaz Nunzio Giudice Maxim Gonchar 龚光华宫辉 Yuri Gornushkin Alexandre Göttel Marco Grassi Christian Grewing Vasily Gromov 顾旻皓谷肖飞古宇关梦云 Nunzio Guardone Maria Gul 郭聪郭竞渊郭万磊郭新恒郭宇航 Paul Hackspacher Caren Hagner 韩然 Yang Han Muhammad Sohaib Hassan 何苗何伟 Tobias Heinz Patrick Hellmuth 衡月昆 Rafael Herrera 贺远强侯少静 Yee Hsiung Bei-Zhen Hu 胡航胡健润胡俊胡守扬胡涛胡宇翔胡焯钧黄春豪黄桂鸿黄翰雄黄文昊黄鑫黄性涛黄永波惠加琪霍雷霍文驹 Cédric Huss Safeer Hussain Ara Ioannisian Roberto Isocrate Beatrice Jelmini Kuo-Lun Jen Ignacio Jeria 季筱璐吉星曌贾慧慧贾俊基蹇司玉蒋荻蒋炜江晓山金如意荆小平 Cécile Jollet Jari Joutsenvaara Sirichok Jungthawan Leonidas Kalousis Philipp Ka Pontificia Universidad Católica de Chile SantiagoChile IPHC Universitéde StrasbourgCNRS/IN2P3F-67037 StrasbourgFrance Pakistan Institute of Nuclear Science and Technology IslamabadPakistan INFN Catania and Dipartimento di Fisica e Astronomia dell Universitàdi Catania CataniaItaly East China University of Science and Technology ShanghaiChina University of Science and Technology of China HefeiChina Joint Institute for Nuclear Research DubnaRussia INFN Sezione di Milano and Dipartimento di Fisica dell Universitàdi Milano MilanoItaly Department of Physics Faculty of ScienceChulalongkorn UniversityBangkokThailand IJCLab UniversitéParis-SaclayCNRS/IN2P391405 OrsayFrance Department of Physics and Earth Science University of Ferrara and INFN Sezione di FerraraFerraraItaly INFN Milano Bicocca and University of Milano Bicocca MilanoItaly INFN Sezione di Padova PadovaItaly III.Physikalisches Institut B RWTH Aachen UniversityAachenGermany Eberhard Karls Universität Tübingen Physikalisches InstitutTübingenGermany Institute of High Energy Physics BeijingChina SUBATECH Universitéde NantesIMT AtlantiqueCNRS-IN2P3NantesFrance Univ.Bordeaux CNRSLP2IUMR 5797F-33170 GradignanFrance Department of Physics National Taiwan UniversityTaipei Dipartimento di Fisica e Astronomia dell’Universitàdi Padova and INFN Sezione di Padova PadovaItaly University of Roma Tre and INFN Sezione Roma Tre RomaItaly Aix Marseille Univ CNRS/IN2P3CPPMMarseilleFrance Wuhan University WuhanChina INFN Milano Bicocca and Politecnico of Milano MilanoItaly National United University Miao-Li Dongguan University of Technology DongguanChina Tsinghua University BeijingChina Institute of Modern Physics Chinese Academy of SciencesLanzhouChina Institute of Physics National Yang Ming Chiao Tung UniversityHsinchu North China Electric Power University BeijingChina Sun Yat-Sen University GuangzhouChina Beijing Institute of Spacecraft Environment Engineering BeijingChina Universidade Estadual de Londrina LondrinaBrazil INFN Sez

The Jiangmen Underground Neutrino Observatory(JUNO)is a large liquid scintillator detector designed to explore many topics in fundamental *** this study,the potential of searching for proton decay in the p→νK^(+)mode with JUNO is *** kaon and its decay particles feature a clear three-fold coincidence signature that results in a high efficiency for ***,the excellent energy resolution of JUNO permits suppression of the sizable background caused by other delayed *** on these advantages,the detection efficiency for the proton decay via p→νK^(+)is 36.9%±4.9%with a background level of 0.2±0.05(syst)±0.2(stat)events after 10 years of data *** estimated sensitivity based on 200 kton-years of exposure is 9.6×1033 years,which is competitive with the current best limits on the proton lifetime in this channel and complements the use of different detection technologies.

关键词： proton decay grand unified theories JUNO liquid scintillator detector

Performance and calibration of quark/gluon-jet taggers using 140 fb^(-1) of pp collisions at √s=13 TeV with the ATLAS detector

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Chinese Physics C 2024年第2期48卷 1-25页

作者： G. Aad B. Abbott K. Abeling N.J. Abicht S.H. Abidi A. Aboulhorma H. Abramowicz H. Abreu Y. Abulaiti B.S. Acharya C. Adam Bourdarios L. Adamczyk S.V. Addepalli M.J. Addison J. Adelman A. Adiguzel T. Adye A.A. Affolder Y. Afik M.N. Agaras J. Agarwala A. Aggarwal C. Agheorghiesei A. Ahmad F. Ahmadov W.S. ahmed S. Ahuja X. Ai G. Aielli A. Aikot M. Ait Tamlihat B. Aitbenchikh I. Aizenberg M. Akbiyik T.P.A. Åkesson A.V. Akimov D. Akiyama N.N. Akolkar K. Al Khoury G.L. Alberghi J. Albert P. Albicocco G.L. Albouy S. Alderweireldt M. Aleksa I.N. Aleksandrov C. Alexa T. Alexopoulos F. Alfonsi M. Algren M. Alhroob B. Ali H.M.J. Ali S. Ali S.W. Alibocus M. Aliev G. Alimonti W. Alkakhi C. Allaire B.M.M. Allbrooke J.F. Allen C.A. Allendes Flores P.P. Allport A. Aloisio F. Alonso C. Alpigiani M. Alvarez Estevez A. Alvarez Fernandez M. Alves Cardoso M.G. Alviggi M. Aly Y. Amaral Coutinho A. Ambler C. Amelung M. Amerl C.G. Ames D. Amidei S.P. Amor Dos Santos K.R. Amos V. Ananiev C. Anastopoulos T. Andeen J.K. Anders S.Y. Andrean A. Andreazza S. Angelidakis A. Angerami A.V. Anisenkov A. Annovi C. Antel M.T. Anthony E. Antipov M. Antonelli F. Anulli M. Aoki T. Aoki J.A. Aparisi Pozo M.A. Aparo L. Aperio Bella C. Appelt A. Apyan N. Aranzabal S.J. Arbiol Val C. Arcangeletti A.T.H. Arce E. Arena J-F. Arguin S. Argyropoulos J.-H. Arling O. Arnaez H. Arnold G. Artoni H. Asada K. Asai S. Asai N.A. Asbah J. Assahsah K. Assamagan R. Astalos S. Atashi R.J. Atkin M. Atkinson H. Atmani P.A. Atmasiddha K. Augsten S. Auricchio A.D. Auriol V.A. Austrup G. Avolio K. Axiotis G. Azuelos D. Babal H. Bachacou K. Bachas A. Bachiu F. Backman A. Badea P. Bagnaia M. Bahmani A.J. Bailey V.R. Bailey J.T. Baines L. Baines O.K. Baker E. Bakos D. Bakshi Gupta V. Balakrishnan R. Balasubramanian E.M. Baldin P. Balek E. Ballabene F. Balli L.M. Baltes W.K. Balunas J. Balz E. Banas M. Bandieramonte A. Bandyopadhyay S. Bansal L. Barak M. Barakat E.L. Barberio D. Barberis M. Barbero M.Z. Barel K.N. Barends T. Barillari M-S. Barisits T. Barklow P. Baron D.A. Baron Mo The ATLAS Collaboration

The identification of jets originating from quarks and gluons,often referred to as quark/gluon tagging,plays an important role in various analyses performed at the Large Hadron Collider,as Standard Model measurements and searches for new particles decaying to quarks often rely on suppressing a large gluon-induced *** paper describes the measurement of the efficiencies of quark/gluon taggers developed within the ATLAS Collaboration,using √s=13 TeV proton–proton collision data with an integrated luminosity of 140 fb^(-1) collected by the ATLAS *** taggers with high performances in rejecting jets from gluon over jets from quarks are studied:one tagger is based on requirements on the number of inner-detector tracks associated with the jet,and the other combines several jet substructure observables using a boosted decision tree.A method is established to determine the quark/gluon fraction in data,by using quark/gluon-enriched subsamples defined by the jet *** in tagging efficiency between data and simulation are provided for jets with transverse momentum between 500 GeV and 2 TeV and for multiple tagger working points.

关键词： ATLAS JET QUARK GLUON TAGGING