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Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections

Light(Science & Applications) 2022年第3期11卷 35-51页

作者： Luis E.Villegas-Hernández Vishesh Dubey Mona Nystad Jean-Claude Tinguely David A.Coucheron Firehun T.Dullo Anish Priyadarshi Sebastian Acuña Azeem Ahmad JoséM.Mateos Gery Barmettler urs ziegler Åsa Birna Birgisdottir Aud-Malin Karlsson Hovd Kristin Andreassen Fenton Ganesh Acharya Krishna Agarwal Balpreet Singh Ahluwalia Department of Physics and Technology UiT The Arctic University of NorwayKlokkargårdsbakken N-9019TromsøNorway Department of Clinical Medicine Women’s Health and Perinatology Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Obstetrics and Gynecology University Hospital of North NorwayTromsøNorway Center for Microscopy and Image Analysis University of ZurichZürichSwitzerland Division of Cardiothoracic and Respiratory Medicine University Hospital of North NorwayTromsøNorway Department of Clinical Medicine Clinical Cardiovascular Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Medical Biology RNA and Molecular Pathology Research GroupUiT The Arctic University of NorwayTromsøNorway Division of Obstetrics and Gynecology Department of Clinical ScienceIntervention and TechnologyKarolinska InstituteStockholmSweden

Histology involves the observation of structural features in tissues using a *** diffraction-limited optical microscopes are commonly used in histological investigations,their resolving capabilities are insufficient to visualize details at subcellular *** a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases,the system complexity,high operating cost,lack of multi-modality,and low-throughput imaging of these methods limit their wide adoption for histological *** this study,we introduce the photonic chip as a feasible high-throughput microscopy platform for super-resolution imaging of histological *** cryopreserved ultrathin tissue sections of human placenta,mouse kidney,pig heart,and zebrafish eye retina prepared by the Tokuyasu method,we demonstrate diverse imaging capabilities of the photonic chip including total internal reflection fluorescence microscopy,intensity fluctuation-based optical nanoscopy,single-molecule localization microscopy,and correlative light-electron *** results validate the photonic chip as a feasible imaging platform for tissue sections and pave the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples both in research and clinical settings.

关键词： resolution modal feasible

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