Chip-based multimodal super-resolution microscopy for histological investigations of cryopreserved tissue sections
作者机构：Department of Physics and TechnologyUiT The Arctic University of NorwayKlokkargårdsbakken N-9019TromsøNorway Department of Clinical MedicineWomen’s Health and Perinatology Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Obstetrics and GynecologyUniversity Hospital of North NorwayTromsøNorway Center for Microscopy and Image AnalysisUniversity of ZurichZürichSwitzerland Division of Cardiothoracic and Respiratory MedicineUniversity Hospital of North NorwayTromsøNorway Department of Clinical MedicineClinical Cardiovascular Research GroupUiT The Arctic University of NorwayTromsøNorway Department of Medical BiologyRNA and Molecular Pathology Research GroupUiT The Arctic University of NorwayTromsøNorway Division of Obstetrics and GynecologyDepartment of Clinical ScienceIntervention and TechnologyKarolinska InstituteStockholmSweden
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2022年第11卷第3期
页 面：35-51页
核心收录：
基 金：BSA acknowledges the funding from the Research Council of Norway project#NANO 2021-288565 and project#BIOTEK 2021-285571
主 题：resolution modal feasible
摘 要：Histology involves the observation of structural features in tissues using a *** diffraction-limited optical microscopes are commonly used in histological investigations,their resolving capabilities are insufficient to visualize details at subcellular *** a novel set of super-resolution optical microscopy techniques can fulfill the resolution demands in such cases,the system complexity,high operating cost,lack of multi-modality,and low-throughput imaging of these methods limit their wide adoption for histological *** this study,we introduce the photonic chip as a feasible high-throughput microscopy platform for super-resolution imaging of histological *** cryopreserved ultrathin tissue sections of human placenta,mouse kidney,pig heart,and zebrafish eye retina prepared by the Tokuyasu method,we demonstrate diverse imaging capabilities of the photonic chip including total internal reflection fluorescence microscopy,intensity fluctuation-based optical nanoscopy,single-molecule localization microscopy,and correlative light-electron *** results validate the photonic chip as a feasible imaging platform for tissue sections and pave the way for the adoption of super-resolution high-throughput multimodal analysis of cryopreserved tissue samples both in research and clinical settings.
维普期刊数据库