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Genes & Diseases 2022年第6期9卷 1506-1520页

作者： Jon Christiansen Anne-Katrin Guttsches ulrike schara-schmidt Matthias Vorgerd Christoph Heute Corinna Preusse Werner Stenzel Andreas Roos Department of Pediatric Neurology Centre for Neuromuscular DisordersCentre for Translational Neuro-and Behavioral SciencesUniversity Hospital EssenUniversity Duisburg-Essen45147 EssenGermany Department of Neurology Heimer Institute for Muscle ResearchUniversity Hospital BergmannsheilRuhr University Bochum44789 BochumGermany Department of Adult and Pediatric Diagnostic Interventional RadiologyNuclear MedicineBG University Hospital Bergmannsheil Bochum44789 BochumGermany Charite´e Universitatsmedizin Berlin Freie Universitat Berlin10117 BerlinGermany Humboldt-Universitat zu Berlin Department of Neuropathology10117 BerlinGermany Department of Neurology with Institute for Translational Neurology University Hospital MunsterAlbert-Schweitzer-Campus 148149 MunsterGermany Children’s Hospital of Eastern Ontario Research Institute University of OttawaOttawaON K1H 8L1Canada

Anoctamin-5(ANO5)is a multi-pass membrane protein localized to the sarcolemma and the sarcoplasmic *** were linked to rare autosomal recessive muscle ***,we summarize the clinical spectrum,imaging data and molecular research findings as well as results of animal modeling,which significantly moved forward the understanding of mechanisms underlying ANO5-related muscle *** that precise histological information on inflammatory processes taking place in patient-derived muscle are still lacking,an(immuno)histological study on biopsies derived from six ANO5-patients was performed showing focal accumulation of necrotic fibers,mild fiber-size variances and *** addition,MRI data of four ANO5-patients(including a 10-year follow-up in one patient)are presented and discussed in the context of previously published ***,data presented in this article combining a review of the literature with own myopathological findings address scientific trends and open questions on ANO5-related muscle diseases,which would be of significant interest for a wide neuromuscular diseases *** conclude,a clear genotypeephenotype correlation does not exist,and ANO5-related muscle disorders might represent the next entity of a clinical continuum with varying degree of muscle cell *** addition,results of pre-clinical studies allowed the definition of suitable cell and animal models characterized by certain histological and functional pathologies resembling the human *** models might serve as suitable systems for testing of interventional concepts in future.

关键词： Anoctamin-5 LGMDR12 MMD3 Muscle inflammation Muscle MRI

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The Pichia pastoris enzyme production platform:From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass

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Journal of Bioresources and Bioproducts 2024年第1期9卷 43-57页

作者： Korbinian Sinzinger ulrike Obst Samed Güner Manuel Döring Magdalena Haslbeck Doris Schieder Volker Sieber Chair of Chemistry of Biogenic Resources Campus Straubing for Biotechnology and SustainabilityTechnical University of MunichStraubing 94315Germany Catalysis Research Center Technical University of MunichGarching 85748Germany SynBiofoundry@TUM Technical University of MunichStraubing 94315Germany School of Chemistry and Molecular Biosciences The University of QueenslandSt.Lucia 4072Australia

The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial ***,making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a ***,the production of the enzymes themselves comes with unavoidable impacts,e.g.,the need to utilize secondary ***,we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris *** demonstrate these capabilities by producing a phytase secreted by *** that is grown on cyanobacterium hydrolysate and buffered glycerol-complex(BMGY)medium,with genetic expression conditions identified by high-throughput screening of a randomized se-cretion *** our best-performing strain is grown in a fed-batch fermentation on BMGY,we reach over 7000 U/mL in three days.

关键词： Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris

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Small mammal community composition impacts bank vole (Clethrionomys glareolus) population dynamics and associated seroprevalence of Puumala orthohantavirus

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Integrative Zoology 2024年第1期19卷 52-65页

作者： Felicitas Maria BUJNOCH Daniela REIL Stephan DREWES ulrike MROSENFELD Rainer G.ULRICH Jens JACOB Christian IMHOLT Julius Kühn-Institute Federal Research Centre for Cultivated PlantsInstitute for Epidemiology and Pathogen DiagnosticsRodent ResearchMünsterGermany University of Münster Institute for Evolution and BiodiversityMünsterGermany Friedrich-Loeffler-Institut Federal Research Institute for Animal HealthInstitute of Novel and Emerging Infectious DiseasesGreifswald-Insel RiemsGermany

Rodents are important reservoirs for zoonotic pathogens that cause diseases in *** is hypoth-esized to be closely related to pathogen prevalence through multiple direct and indirect *** example,the presence of non-host species can reduce contact rates of the main reservoir host and thus reduce the risk of transmission(“dilution effect”).In addition,an overlap in ecological niches between two species could lead to increased interspecific competition,potentially limiting host densities and reducing density-dependent pathogen transmission *** this study,we investigated the relative impact of population-level regulation of direct and indirect drivers of the prevalence of Puumala orthohantavirus(PUUV)in bank voles(Clethrionomys glareolus)during years with high *** compiled data on small mammal community composition from four regions in Germany between 2010 and *** equation modeling revealed a strong seasonality in PUUV control mechanisms in bank *** abundance of shrews tended to have a negative relationship with host abundance,and host abundance positively influenced PUUV seroprevalence,while at the same time increasing the abundance of competing non-hosts like the wood mouse(Apodemus sylvaticus)and the yellow-neckedfield mouse(Apodemusflavicollis)were associated with reduced PUUV seroprevalence in the *** results indicate that for PUUV in bank voles,dilution is associated with increased interspecific *** pressures leading to the decline of Apodemus *** a specific habitat could lead to the amplification of mechanisms promoting PUUV transmission within the host populations.

关键词： bank vole biodiversity Puumala orthohantavirus small mammals

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湖泊沉积植物DNA宏条形码技术在青藏高原植被调查中的应用

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中国科学:地球科学 2024年

作者：吴铠李凯贾伟瀚 Kathleen R.STOOF-LEICHSENRING ulrike HERZSCHUH 倪健廖梦娜田芳浙江师范大学生命科学学院 Polar Terrestrial Environmental Systems Research Group Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Institute of Environmental Science and Geography University of Potsdam Institute of Biochemistry and Biology University of Potsdam 首都师范大学资源环境与旅游学院

得益于环境DNA技术的快速发展,沉积DNA(sedDNA)正在成为偏远地区植被监测和调查的便捷方法,并具有较大的发展潜力.青藏高原气候环境条件恶劣,同时小型湖泊众多,利用sedDNA进行植被调查不仅有助于了解高原现代植被状况,也是进行高原古植... 详细信息

得益于环境DNA技术的快速发展,沉积DNA(sedDNA)正在成为偏远地区植被监测和调查的便捷方法,并具有较大的发展潜力.青藏高原气候环境条件恶劣,同时小型湖泊众多,利用sedDNA进行植被调查不仅有助于了解高原现代植被状况,也是进行高原古植被重建的潜在需求.本研究以中国青藏高原西南部59个泡子/湖泊表层沉积物为研究对象,使用通用植物引物g-h扩增叶绿体trn L(UAA)内含子P6环区,获取了sedDNA中的植物组成信息,并将其与野外植被调查记录和沉积物花粉数据进行对比分析,探讨了基于沉积物的植物DNA宏条形码技术在青藏高原地区现代植被监测和古植被重建中的适用性和局限性.研究结果表明,植物DNA宏条形码信号共记录了186个陆生植物类群,其中30.1%的序列可以鉴定到种水平.植物sedDNA方法可以揭示调查地点周围的主要植物类群(包括菊科、莎草科和禾本科)以及重要植被组合.植物sedDNA的类群数量和分类学分辨率均超过花粉分析(75个类群,其中5.3%的类群可以鉴定到种水平).与花粉保留大量的区域植物信号(包括松属和蒿属)不同,植物sedDNA反映了高度局地的植物信号,凸显了植物sedDNA在局地植被监测和重建中的重要性.综上所述,(小型)湖泊植物sedDNA宏条形码研究在未来的青藏高原区域植被监测和重建中值得更多的关注.

关键词：沉积DNA 宏条形码花粉植被组成青藏高原

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An orally-available monovalent SMAC mimetic compound as a broad-spectrum antiviral

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Protein & Cell 2024年第1期15卷 69-75页

作者： Miao Mei Maria_Antonietta Impagnatiello Jun Jiao Ulrich Reiser ulrike Tontsch-Grunt Ju Zhang Paul Nicklin Bingke Yu Yu Wang Yuan He Xu Tan Tsinghua-Peking Center for Life Sciences Beijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureMOE Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical BiologySchool of Pharmaceutical SciencesTsinghua UniversityBeijing 100084China Chinese Institutes for Medical Research Beijing 100069China Boehringer Ingelheim RCV GmbH&Co KG ViennaAustria College of Life Sciences and Oceanography Shenzhen UniversityShenzhen 518060China Research Beyond Borders Boehringer IngelheimBiberach an der RissGermany Research Beyond Borders Boehringer IngelheimShanghai 200120China

DearEditor,The coronavirus disease 2019(COVID-19)pandemic caused great global morbidity and mortality concurrent with astronomical socioeconomic damages and *** now,the culpable pathogen,SARS-CoV-2,has infected over 6 hundred million people resulting in more than 6 million deaths worldwide(WHO SARSCoV-2 cases).There is an urgent need for rapid development of effective and affordable therapeutics for COVID-19 patients.

关键词： mortality spectrum compound

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Application of plant DNA metabarcoding of lake sediments for monitoring vegetation compositions on the Tibetan Plateau

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Science China Earth Sciences 2024年

作者： Kai WU Kai LI Weihan JIA Kathleen R.STOOF-LEICHSENRING ulrike HERZSCHUH Jian NI Mengna LIAO Fang TIAN College of Life Sciences Zhejiang Normal University Polar Terrestrial Environmental Systems Research Group Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Institute of Environmental Science and Geography University of Potsdam Institute of Biochemistry and Biology University of Potsdam College of Resource Environment and Tourism Capital Normal University

Benefiting from the rapid development of environmental DNA (eDNA) technologies,sedimentary DNA (sedDNA)emerges as a promising tool for monitoring plant compositions in remote *** Tibetan Plateau (TP),renowned for its harsh environment and numerous ponds and lakes,presents a potentially demanding region for the application of sedDNA on vegetation ***,we used the g and h universal primers for the P6 loop region of the chloroplast trn L (UAA)intron to amplify plant DNA in surface sediments from 59 ponds and small lakes on the southwestern *** applicability and limitations of using plant DNA metabarcoding for modern vegetation monitoring and palaeo-vegetation reconstructions have been assessed by comparing sedDNA,pollen,and vegetation survey *** results showed that plant DNA metabarcoding recorded 186 terrestrial taxa,of which 30.1%can be identified at the species *** plant sedDNA approach can effectively disclose the dominant plant taxa (including Asteraceae,Cyperaceae and Poaceae) and significant vegetation assemblages in the vicinity of the investigated *** number of taxa and taxonomic resolution of plant sedDNA exceeded that of pollen analysis(75 taxa detected,5.3%can be identified at species level).Unlike pollen that retains a broad spectrum of regional plant signals(including Pinus and Artemisia),plant sedDNA mirrors very local plants,underscoring its utility in local vegetation monitoring and *** conclude,plant DNA metabarcoding of (small) lake sediments warrant increased attention in the future for local vegetation monitoring and reconstructions on the TP.

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Development of an active-site titrant for SARS-CoV-2 main protease as an indispensable tool for evaluating enzyme kinetics

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Acta Pharmaceutica Sinica B 2024年第5期14卷 2349-2357页

作者： Rabea Voget Julian Breidenbach Tobias Claff Alexandra Hingst Katharina Sylvester Christian Steinebach Lan Phuong Vu Renato H.Weiße ulrike Bartz Norbert Sträter Christa E.Müller Michael Gütschow Pharmaceutical Institute Pharmaceutical&Medicinal ChemistryUniversity of BonnBonn 53121Germany Institute of Bioanalytical Chemistry Center for Biotechnology and BiomedicineLeipzig UniversityLeipzig 04103Germany Department of Natural Sciences University of Applied Sciences Bonn-Rhein-SiegRheinbach 53359Germany

A titrant for the SARS-CoV-2 main protease(M^(pro))was developed that enables,for the first time,the exact determination of the concentration of the enzymatically active M^(pro) by active-site *** covalent binding mode of the tetrapeptidic titrant was elucidated by the determination of the crystal structure of the enzyme–titrant *** fluorogenic substrates of M^(pro),including a prototypical,internally quenched Dabcyl-EDANS peptide,were compared in terms of solubility under typical assay *** exploiting the new titrant,key kinetic parameters for the M^(pro)-catalyzed cleavage of these substrates were determined.

关键词： COVID-19 SARS-CoV-2 Main protease Peptide nitriles Fluorogenic substrates Active-site titration X-ray crystallography Inner filter effect

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Intercultural Trust in Global Contexts:Synthesizing a Western Nomological Approach with a Chinese Systems Approach

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Journal of Systems Science and Systems Engineering 2024年第2期33卷 162-186页

作者： Rong Du Mingqian Li Shizhong Ai Cathal MacSwiney Brugha Uirike Reisach School of Economics and Management Xidian UniversityXi'an 710071China UCD School of Business University College DublinDublinIreland Information Management Department Neu-Ulm University of Applied SciencesBavariaGermany

Intercultural trust in global contexts plays a central role in helping people from different cultures to communicate comfortably,which is essential for *** to construct a framework that might foster international cooperation,and thus be helpful for coping with global emergencies,we relate a Western nomological approach to an Eastern systems approach to analyse intercultural trust in global *** cultural impacts on intercultural trust and the nomological framework of cultural differences,we propose an intercultural trust model to interpret how cultural differences influence trust.A qualitative study of Chinese-Irish interactions was conducted to interpret this *** organized 10 seminars on intercultural trust,and interviewed 16 people to further explore the respondents'deeper feelings and experiences about intercultural trust in global *** this study,we have identified factors impacting on intercultural trust,and found that intercultural trust can be developed and improved in various *** llustrate these ways,we have provided tactics and methods for building intercultural trust in global *** are highlighted for organizations to avoid cultural clashes and relevant political or economic risks.

关键词： Intercultural trust global contexts systems approach Western approach Chinese approach

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Complementarity of lacustrine pollen and sedimentary DNA in representing vegetation on the central-eastern Tibetan Plateau

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Frontiers of Earth Science 2023年第4期17卷 1037-1048页

作者： Fang TIAN Meijiao CHEN Weihan JIA ulrike HERZSCHUH Xianyong CAO College of Resource Environment and Tourism Capital Normal UniversityBeijing 100048China Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research Polar Terrestrial Environmental Systems Research GroupPotsdam 14473Germany University of Potsdam Institute of Environmental Science and GeographyPotsdam 14476Germany University of Potsdam Institute of Biochemistry and BiologyPotsdam 14476Germany Group of Alpine Paleoecology and Human Adaptation(ALPHA) State Key Laboratory of Tibetan Plateau Earth SystemEnvironment and Resources(TPESER)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China

Plant environmental DNA extracted from lacustrine sediments(sedimentary DNA,sedDNA)has been increasingly used to investigate past vegetation changes and human impacts at a high taxonomic ***,the representation of vegetation communities surrounding the lake is still *** this study,we compared plant sedDNA metabarcoding and pollen assemblages from 27 lake surface-sediment samples collected from alpine meadow on the central-eastern Tibetan Plateau to investigate the representation of sedDNA *** general,the identified components of sedDNA are consistent with the counted pollen taxa and local plant *** to pollen identification,sedDNA data have higher taxonomic resolution,thus providing a potential approach for reconstructing past plant *** sedDNA signal is strongly influenced by local plants while rarely affected by exogenous *** of the overrepresentation of local plants and PCR bias,the abundance of sedDNA sequence types is very variable among sites,and should be treated with caution when investigating past vegetation cover and climate based on sedDNA *** finding suggests that sedDNA analysis can be a complementary approach for investigating the presence/absence of past plants and history of human land-use with higher taxonomic resolution.

关键词： Tibetan Plateau plant DNA metabarcoding pollen lake sediment plant diversity vegetation history

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Machine learning for exploring small polaron configurational space

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npj Computational Materials 2022年第1期8卷 1183-1191页

作者： Viktor C.Birschitzky Florian Ellinger ulrike Diebold Michele Reticcioli Cesare Franchini University of Vienna Faculty of Physics and Center for Computational Materials ScienceViennaAustria University of Vienna Vienna Doctoral School in PhysicsBoltzmanngasse 51090ViennaAustria Institute of Applied Physics Technische Universität Wien1040ViennaAustria Department of Physics and Astronomy’Augusto Righi’ Alma Mater Studiorum-Universitàdi BolognaBologna40127Italy

Polaron defects are ubiquitous in materials and play an important role in many processes involving carrier mobility,charge transfer and surface *** small polarons’spatial distributions is essential to understand materials properties and ***,the required exploration of the configurational space is computationally demanding when using first principles ***,we propose a machine-learning(ML)accelerated search that determines the ground state polaronic *** ML model is trained on databases of polaron configurations generated by density functional theory(DFT)via molecular dynamics or random *** establish a mapping between configurations and their stability,we designed descriptors modelling the interactions among polarons and charged point *** used the DFT+ML protocol to explore the polaron configurational space for two surface-systems,reduced rutile TiO_(2)(110)and Nb-doped SrTiO_(3)(001).The ML-aided search proposes additional polaronic configurations and can be utilized to determine optimal polaron distributions at any charge concentration.

关键词： configuration polaron charge

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