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Journal of Bioresources and Bioproducts 2024年第1期9卷 43-57页

作者： Korbinian Sinzinger ulrike obst Samed Güner Manuel Döring Magdalena Haslbeck Doris Schieder Volker Sieber Chair of Chemistry of Biogenic Resources Campus Straubing for Biotechnology and SustainabilityTechnical University of MunichStraubing 94315Germany Catalysis Research Center Technical University of MunichGarching 85748Germany SynBiofoundry@TUM Technical University of MunichStraubing 94315Germany School of Chemistry and Molecular Biosciences The University of QueenslandSt.Lucia 4072Australia

The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial processes.However,making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a challenge.Moreover,the production of the enzymes themselves comes with unavoidable impacts,e.g.,the need to utilize secondary feedstocks.Here,we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris fermentation.We demonstrate these capabilities by producing a phytase secreted by P.pastoris that is grown on cyanobacterium hydrolysate and buffered glycerol-complex(BMGY)medium,with genetic expression conditions identified by high-throughput screening of a randomized se-cretion library.When our best-performing strain is grown in a fed-batch fermentation on BMGY,we reach over 7000 U/mL in three days.

关键词： Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris

Extending the production boundary of the System of National Accounts (SNA) to classify and account for ecosystem services

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Ecosystem Health and Sustainability 2018年第11期4卷 19-32页

作者： Mark Eigenraam Carl obst Institute for the Development of Environmental-Economic Accounting(IDEEA) MelbourneAustralia

There is a broad acceptance to depicting the relationship between ecosystems and human well-being using the concept of ecosystem services,emanating in large from the findings and research published in the Millennium Ecosystem Assessment in 2005.While the generic concept of ecosystem services provides an excellent platform for discussion,the ongoing lack of clarity surrounding the definition,clas sification and measurement of ecosystem services,is emerging as a barrier to more extensive collaboration across disciplines.This paper applies the principles of national accounting to bring additional rigor and consistency to the discussion on ecosystem services.In this paper we revisit four fundamental aspects of the System of National Accounts(SNA)that underpin the measurement of the economy,namely,the definition of economic units;the definition of production;the recording of transactions and the recording assets.By considering each of these aspects in the context of the United Nations'System of Environmental-Economic Accounting,the paper presents a framework to describe the relationship between ecosystems and human aativity that can then be used to consistently define,classify,measure and account for ecosystem services.

关键词： Ecosystem accounting system of national accounts ecosystem services ecosystem asset environmental accounting

Accounting for liabilities related to ecosystem degradation

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Ecosystem Health and Sustainability 2018年第11期4卷 2-17页

作者： Sue Ogilvy Roger Burritt Dionne Walsh Carl obst Peter Meadows Peter Muradzikwa Mark Eigenraam Fenner School of Environment&Society Australian National UniversityCanberraAustralia Northern Territory Department of Primary Industry&Resources DarwinAustralia IDEEA Group FairfieldAustralia Melbourne Sustainable Society Institute The University of MelbourneParkilleAustralia Australian Bureau of Statistics BelconnenAustralia

Introduction:A growing belief that accounting can and should play a role in halting and reversing degradation of ecosystems is leading to conceptual and methodological develop-ments that recognize the cost of degradation,attribute the cost to the entities responsible and assure that entities can't ignore the economic burden associated with it.Outcome:Demonstration accounts prepared around a scenario where agricultural use of land includes an obligation to maintain ecosystem condition.The accounts are compliant and coherent with both the intermational accounting standards for individual entities and the United Nations'System of Environmental-Economic Accounting.Discussion:Accounting for labilities for ecosystem degradation demonstrates that,where the liability reflects the lost economic value of the ecosystem,the accounts communicate a reduction in the total net worth of the economy and a redistribution of net worth away from the party responsible for the degradation.The inclusion of both libilties for degradation and the cost of degradation does not lead to double-counting the economic impact of degradation.Conclusion:Accounting principles and frameworks encourage greater accountability for entities responsible for ecosystem condition by providing greater visibility of the economic cost to individual entities,governments and nations.

关键词： Ecosystem accounting liabilities degradation sustainability sustainable development goals(SDG)

Mafic Dykes on Bornholm(Denmark) as Indicators of Precambrian to Permian Extensional Events at the SW Margin of the Fennoscandian Shield

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Acta Geologica Sinica(English Edition) 2016年第S1期90卷 100-页

作者： Karsten obst Institute of Geography and Geology University of Greifswald

About 200 mafic dykes intruded Mesoproterozoic gneisses and granites of Bornholm,a Danish island situated in the southern Baltic Sea between Sweden,Germany and Poland.Field studies along the northern coast document

关键词： as Indicators of Precambrian to Permian Extensional Events at the SW Margin of the Fennoscandian Shield Mafic Dykes on Bornholm Denmark

氧化镁在氧气顶吹转炉初渣中的溶解行为与饱和含量

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国外钢铁钒钛 1990年第1期3卷 71-78页

作者： obst.,KH 单麟天

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绵羊生产力的标准—每公顷配种体重

国外畜牧学（草食家畜） 1992年第3期 20-23页

作者： J.M.obst 刘建设

绵羊体重变化和每公顷绵羊只数是促使绵羊生产率改变的两个主要因素。单产一直是评定和选择种羊场和商品羊场毛用羊生产力的主要标准。随着载畜量的增加,每只羊的增重呈直线下降。Jones等(1974)在肉牛和绵羊中已算出每公顷体增重和载畜... 详细信息

绵羊体重变化和每公顷绵羊只数是促使绵羊生产率改变的两个主要因素。单产一直是评定和选择种羊场和商品羊场毛用羊生产力的主要标准。随着载畜量的增加,每只羊的增重呈直线下降。Jones等(1974)在肉牛和绵羊中已算出每公顷体增重和载畜量之间的曲线相关。最近的研究(obst,1987,obst等,1991)已强调说明,绵羊生产力的标准应该是每公顷生产力,而不是每只的生产性能。潜在绵羊生产力的上下限主要是根据草场生产力水平和草场利用程度设定的。

关键词：绵羊生产力配种羊

微型浮游生物应为湖泊中的方解石沉淀负责吗?

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AMBIO－人类环境杂志 2004年第B12期33卷 535-540页

作者： Maria Dittrich Martin obst 张国梁（译）盖峥（校） EAWAG的瑞士联邦环境科学拜鲁特大学不详

湖泊中方解石的沉积是环境变迁的重要记录。为了解译这些档案,有必要了解方解石的起源。普遍认为方解石的沉淀能被细菌和藻类所诱导。然而,详细的机制仍不清楚。本综述概括了已知的在方解石沉淀和自养微型浮游生物之间的相互作用。我们... 详细信息

湖泊中方解石的沉积是环境变迁的重要记录。为了解译这些档案,有必要了解方解石的起源。普遍认为方解石的沉淀能被细菌和藻类所诱导。然而,详细的机制仍不清楚。本综述概括了已知的在方解石沉淀和自养微型浮游生物之间的相互作用。我们考虑了来自野外和实验室研究两方面的发现。野外研究显示,在贫营养湖中方解石的沉淀与微型蓝细菌的繁盛有密切的关系。室内实验使微藻类诱导沉淀的机制清晰化了。实验也显示由微型蓝细菌诱导的沉淀受到各种不同因素的影响,包括无机碳的吸收和细胞壁结构。最近的研究指出还必须考虑环境条件如湖水组成的影响。我们得出结论,需要原位观察受控条件下在微型浮游生物细胞上的沉淀过程以改善我们对矿物细菌相互作用的理解。

关键词：微型浮游生物沉淀过程方解石湖泊实验室研究环境变迁相互作用野外研究细胞壁结构

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低COD污水絮凝工艺模型

环境科学导刊 2020年第1期39卷 59-62页

作者：王卉杨方洲韩小溪王亦民段博宇 Yuri obst 澳大利亚蓝棋环境科技有限公司云南昆明650000 昆明市土壤肥料工作站云南昆明650000 澳大利亚贝林微滤有限公司云南昆明650000

对低COD污水处理絮凝环节中不同影响因素进行分析,构建了模型,并对模型进行统计检验。对比模型预测结果与真实结果,可以看出,该模型能准确指导PAC、PAM的投加并对有机污染物的去除率进行可靠估计。

关键词：污水处理低COD污水 PAC PAM COD去除工艺模型

Study on Lymphokine-Activated Killer Cells Induced from Cord Blood

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Chinese Medical Journal 1994年第8期 18-18页

作者： Wang Yi-feng, et al. Affiliated Hospital, Shandong Med Uni, Jinan 250012. Chin J obst Gynecol 1994

水泡状胎块排空前后单纯阴道转移结节的病理分析

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临床与实验病理学杂志 1989年第2期5卷 82-84页

作者：傅兴生万桂英梁玉屏黄舜琴胡丽莎江西省妇产医院

根据水泡状胎块第一次刮宫、阴道转移结节及切除子宫的病理资料及尿中hCG测定的分析,发现水泡状胎块在排空前发生转移结节的12例,在切除子宫标本中无一例发生滋养细胞及绒毛的深肌层浸润。水泡状胎块排空后30例发生转移结节的切除子宫... 详细信息

根据水泡状胎块第一次刮宫、阴道转移结节及切除子宫的病理资料及尿中hCG测定的分析,发现水泡状胎块在排空前发生转移结节的12例,在切除子宫标本中无一例发生滋养细胞及绒毛的深肌层浸润。水泡状胎块排空后30例发生转移结节的切除子宫标本中,有11例呈深肌层浸润。经统计学处理P<0.05,差异有显著性。尿中hCG测定在排空前发生转移结节11例在4周内转为阴性;排空后发生转移的15例在4～8周转为阴性,4例在8周以上转为阴性,故此认为,单纯阴道转移发生在水泡状胎块发生前,比排空后预后更好。

关键词：水泡状胎块阴道结节病理