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荧光原位杂交法快速鉴定金黄色葡萄球菌和凝固酶阴性葡萄球菌

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中华检验医学杂志 2004年第7期27卷 434-436页

作者：王沛 Welling GW Meessen N Raangs gc 湖北省荆门市第一人民医院检验科 448000 荷兰格罗宁根大学医学微生物系

目的建立一种新的荧光原位杂交法,快速鉴定血培养中的金黄色葡萄球菌(金葡菌)和凝固酶阴性葡萄球菌。方法将荧光同位素标记的针对金葡菌和凝固酶阴性葡萄球菌的核糖体16SrRNA序列的互补的DNA寡核苷酸探针,在同一张玻片上50℃杂交1 h,... 详细信息

目的建立一种新的荧光原位杂交法,快速鉴定血培养中的金黄色葡萄球菌(金葡菌)和凝固酶阴性葡萄球菌。方法将荧光同位素标记的针对金葡菌和凝固酶阴性葡萄球菌的核糖体16SrRNA序列的互补的DNA寡核苷酸探针,在同一张玻片上50℃杂交1 h,用杂交缓冲液50℃洗涤10min,干燥后置荧光显微镜下观察,全过程大约2 h。结果探针的特异性经标准菌株和临床分离菌株证实。将259份临床标本测试与培养法比较,金葡菌的特异性和敏感性均为100％,CoNs的特异性和敏感性分别为100％和95.5％,荧光原位杂交法的最低检出限为103CFU/ml。结论本方法适用于血培养中的金葡菌和凝固酶阴性葡萄球菌的快速鉴定。

关键词：荧光原位杂交法鉴定金黄色葡萄球菌凝固酶阴性葡萄球菌细菌学技术

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Polysubstituted Isoflavonoids from Spatholobus suberectus,Flemingia macrophylla,and Cudrania cochinchinensis

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Natural Products and Bioprospecting 2017年第2期7卷 201-206页

作者： Li-Xia Wang Hai-Rong Zheng Fu-Cai Ren Tian-gc Chen Xiang-Mei Li Xian-Jun Jiang Fei Wang BioBioPha Co. Ltd.Kunming 650201People’s Republic of China

Four hitherto unknown polysubstituted isofiavonoids,including three isofiavans:7,4'-dihydroxy-8,2',3'-trimethoxyisofiavan(1),7,2',4'-trihydroxy-8,3'-dimethoxyisofiavan(2),and 7,2',4'-trihydroxy-5-methoxyisofiavan(3),and one prenylated isofiavone cudraisofiavone M(4)were isolated from the ethanol extracts of Spatholobus suberectus(for 1 and 2),Flemingia macrophylla(for 3),and Cudrania cochinchinensis(for 4),*** structures were established on the basis of extensive spectroscopic *** 1 and 4 exhibited weak cytotoxic activity against five human cancer cell lines(HL-60,A-549,SMMC-7721,MCF-7,and SW-480).

关键词： Spatholobus suberect us Flemingia macrophylla Cudrania cochinchinensis Isofiavan Isofiavone Cytotoxicity

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白血病前期综合征

国外医学（输血及血液学分册） 1989年第3期12卷 146-148页

作者： Bagb.,gc 汤宗章

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注水开发油田的监测技术

油气田开发工程译丛 1992年第6期 2-6页

作者： Thak.,gc 赵奇志

Status of Highly Hazardous Pesticides and Their Mitigation Measures in Asia

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Advances in Entomology 2022年第1期10卷 14-33页

作者： Yubak Dhoj gc Bhakta R. Palikhe Baogen Gu Grenier Beatrice Regional Office for Asia and the Pacific Food and Agriculture Organization of the United Nations (FAO) Bangkok Thailand Pest and Pesticide Management Plant Production and Protection Division Food and Agriculture Organization of the United Nations (FAO) Rome Italy

Pesticide is one of the major inputs in modern agriculture and its uses are increasing annually. Pesticides are seen as inherently benign, in the same way that medicines are. Many local languages even use the same word for “pesticide” and “medicine”. Along with the increasing consumption of pesticides, Highly Hazardous Pesticides (HHPs), which are more toxic are still in widespread use in Asia and constitute a substantial challenge to human health and the environment especially in low- and middle-income countries with less resources, training and capacities to deal with them. Reducing the risks posed by HHPs has become a priority in various international chemicals management policies, and many countries have started taking action accordingly. The objective of the paper is to establish the national status of the use of HHPs in Asia in line with FAO/WHO criteria for HHPs and encourage deliberate consideration of mitigation options for effective management of HHPs. This report is based on the nationally registered pesticide active ingredients and other related information obtained from Pesticide Registration Authorities of 13 Asian countries and research reports of different national and international journals. There are currently about 3557 pesticide products (active ingredients) registered in 13 Asian countries to control pests on crops and for public health uses, and 214 HHPs among the list of registered pesticides still in use in these Asian countries. An analysis of the list of registered formulated products revealed that 61

关键词： HHPs Health Environment Risk Mitigation

Polyurethane-degrading fungi from soils contaminated with rocket propellant and their ability to decompose alkyne terminated polybutadiene with urethane

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Studies in Fungi 2021年第1期6卷 224-239页

作者： Ren gc Pang AM Gao Y Wu SX Ge ZQ Zhang TF Wanasinghe DN Khan S Mortimer PE Xu JC Gui H Center of Excellence in Fungal Research Mae Fah Luang UniversityChiang Rai 57100Thailand Guiyang Nursing Vocational College Guiyang 550081GuizhouChina Honghe Center for Mountain Futures Kunming Institute of BotanyChinese Academy of SciencesHonghe County 654400YunnanChina School of Science Mae Fah Luang UniversityChiang Rai 57100Thailand Science and Technology on Aerospace Chemical Power Laboratory Hubei Institute of Aerospace ChemotechnologyXiangyang441003HubeiChina

A large amount of propellant materials are produced every year,and storage and disposal of these propellant materials seriously contributes to environmental *** terminated polybutadiene with urethane segments(PUPB)is the macromolecule backbone of these propellant materials,and degradation of PUPB is central to the eco-friendly treatment of propellant *** this study,we isolated a polyurethane(PU)-and PUPB-degrading fungus from soils contaminated with rocket propellant,and the fungus H14 was identified as Fusarium solani(Mart.)*** on macro-and micro-morphology as well as phylogenetic *** ability of *** H14 to degrade PU film and PUPB patches was evaluated via mass loss,scanning electron microscopy(SEM)and enzyme production *** loss analyses revealed a 25.8%reduction in mass of PU and 1.3%reduction in mass of PUPB after *** H14 was incubated with PU and PUPB for 90 days,*** found that *** H14 mycelia significantly colonized both PU and *** images showed that the surface of PU films and PUPB patches formed holes,underwent folding and experienced damage as well as irregular fissuring from the erosion of fungal ***,two possible degradative enzymes,lipase and esterase,were produced by *** study opens a new avenue of research for eco-friendly treatments of explosive materials and *** paper represents the first article on the degradation of PUPB patches.

关键词： enzyme Fusarium solani mass loss PU PUPB

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阿联酋国家档案馆档案资源建设分析及思考

档案学通讯 2020年第1期 94-100页

作者：李颖魏歌河北大学管理学院

阿联酋国家档案馆是阿拉伯海湾地区最古老且馆藏最丰富的档案馆。从宏观、中观和微观层面分析其档案资源建设的特点,以及可供借鉴之处,结合我国的国情,对未来我国档案资源建设进行了思考,提出在我国档案资源建设中,应注重宏观层面上对... 详细信息

阿联酋国家档案馆是阿拉伯海湾地区最古老且馆藏最丰富的档案馆。从宏观、中观和微观层面分析其档案资源建设的特点,以及可供借鉴之处,结合我国的国情,对未来我国档案资源建设进行了思考,提出在我国档案资源建设中,应注重宏观层面上对档案资源建设的方向引领和顶层设计,中观层面上积极探索补足档案资源体系短板的创新策略,微观层面上面向技术挑战和技术应用实现数字档案资源的有效积淀。

关键词：档案资源阿联酋数字档案资源

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IL－2与IL－10在LAK细胞活性激活中的相互作用

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国际免疫学杂志 1994年第2期17卷 107-108页

作者： Spagnoli-gc 郝京生董玉环

ＩＬ－２与ＩＬ－１０在ＬＡＫ细胞活性激活中的相互作用〔英〕／ＳｐａｇｎｏｌｉＧＣ…／／ＣｅｌｌＩｍｍｕｎｏｌ．─１９９３，１４６。─３９１～４０５白细胞介素２（ＩＬ－２）单独或与过继性细胞联合应用越来越多地用于肿瘤的... 详细信息

ＩＬ－２与ＩＬ－１０在ＬＡＫ细胞活性激活中的相互作用〔英〕／ＳｐａｇｎｏｌｉＧＣ…／／ＣｅｌｌＩｍｍｕｎｏｌ．─１９９３，１４６。─３９１～４０５白细胞介素２（ＩＬ－２）单独或与过继性细胞联合应用越来越多地用于肿瘤的治疗，但由于严重的毒副作用而限制了...

关键词：细胞活性性细胞生殖细胞 LAK IL 供血员

Effect of soy protein supplementation in patients with chronic hepatitis C:A randomized clinical trial

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World Journal of Gastroenterology 2012年第18期18卷 2203-2211页

作者： Lucivalda PM Oliveira Rosangela P de Jesus Ramona SSB Boulhosa Carlos Mauricio C Mendes Maria Cecilia Gnoatto Denise C Lemaire Maria Betania P Toralles Lourianne N Cavalcante Andre C Lyra Luiz gc Lyra Postgraduate Course in Medicine and Health and Nutrition Science Department Federal University of BahiaBahia 40110-150Brazil Nutrition Science Department Federal University of BahiaBahia 40110-150Brazil Postgraduate Course in Medicine and Health Federal University of BahiaBahia 40110-150Brazil the Medicine and Health Postgraduation program Federal University of BahiaBahia 40110-150Brazil the Radiology Service Prof.Edgard Santos University HospitalFederal University of BahiaBahia 40110-150Brazil Laboratory of Immunology Health Science InstituteFederal University of BahiaBahia 40110-150Brazil Pediatric Department Federal University of BahiaBahia 40110-150Brazil Department of Medicine Division of Gastroenterology and HepatologyFederal University of Bahia and Gastro-hepatology UnitHospital S o Rafael of BahiaBahia 40110-150Brazil the Medicine and Health Postgraduation program Federal University of Bahia and Gastro-hepatology UnitHospital S o Rafael of BahiaBahia 40110-150Brazil

AIM:To evaluate the effects of soy supplementation on insulin resistance,fatty liver and alanine aminotransferase(ALT) levels in non-diabetic patients with chronic hepatitis C(CHC).METHODS:In a prospective,randomized and singleblinded clinical trial,we compared patients with CHC who had casein as a supplement(n = 80)(control group),with patients who consumed a soy supplement diet(n = 80) [intervention group(IG)].Both groups received 32 g/d of protein for 12 ***:Patients' baseline features showed that 48.1% were overweight,43.7% had abdominal fat accumulation,34.7% had hepatic steatosis and 36.3% had an homeostasis model assessment index of insulin resistance(HOMA-IR) ≥ *** analysis showed that protein supplementation diet reduced hepatic steatosis in both groups;however,significant reductions in ALT levels occurred in the soy *** regression modeling indicated that in the presence of severe fibrosis(F3/F4),g glutamyl transferase elevation and high density lipoprotein(HDL) reduction,the intervention group had 75% less chance of developing hepatic steatosis(OR= 0.25;95% CI:0.06-0.82) and 55% less chance of presenting with an ALT level ≥ 1.5 × the upper limit of normal(ULN)(OR = 0.45,95% CI:0.22-0.89).Soy treatment did not have any effect on insulin resistance(OR = 1.92;95% CI:0.80-4.83),which might be attributed to the fact that the HOMAIR values at baseline in most of our patients were in the normal *** hepatic fibrosis,an ALT level > 1.5 × ULN and visceral fat were predictors of an HOMA-IR ≥ *** IG group had a reduced risk of an ALT level > 1.5 × *** HOMA-IR ≥ 3.0 and HDL < 35 mg/dL were also risk factors for increased ***:Soy supplementation decreased ALT levels and thus may improve liver inflammation in hepatitis C virus(HCV) patients;it also reduced hepatic steatosis in a subgroup of patients but did not change insulin *** should be considered in the nutritional care of HCV patients.

关键词： Chronic hepatitis C Soy supplementation Insulin resistance Hepatic steatosis Hepatitis C virus