Polyurethane-degrading fungi from soils contaminated with rocket propellant and their ability to decompose alkyne terminated polybutadiene with urethane

作     者：Ren GC Pang AM Gao Y Wu SX Ge ZQ Zhang TF Wanasinghe DN Khan S Mortimer PE Xu JC Gui H 

作者机构：Center of Excellence in Fungal ResearchMae Fah Luang UniversityChiang Rai 57100Thailand Guiyang Nursing Vocational CollegeGuiyang 550081GuizhouChina Honghe Center for Mountain FuturesKunming Institute of BotanyChinese Academy of SciencesHonghe County 654400YunnanChina School of ScienceMae Fah Luang UniversityChiang Rai 57100Thailand Science and Technology on Aerospace Chemical Power LaboratoryHubei Institute of Aerospace ChemotechnologyXiangyang441003HubeiChina 

出 版 物：《Studies in Fungi》 (真菌研究（英文）)

年 卷 期：2021年第6卷第1期

页      面：224-239页

学科分类：0710[理学-生物学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1203[管理学-农林经济管理] 08[工学] 0903[农学-农业资源与环境] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0706[理学-大气科学] 0901[农学-作物学] 0902[农学-园艺学] 0836[工学-生物工程] 0713[理学-生态学] 

基　　金：This work was financed by Open Research Fund Program of Science and Technology on Aerospace Chemical Power Laboratory(STACPL320181B04) We also would like to thank the support from the National Natural Science Foundation of China(NSFC21975066,NSFC21875061). 

主　　题：enzyme Fusarium solani mass loss PU PUPB 

摘      要：A large amount of propellant materials are produced every year,and storage and disposal of these propellant materials seriously contributes to environmental pollution.Alkyne terminated polybutadiene with urethane segments(PUPB)is the macromolecule backbone of these propellant materials,and degradation of PUPB is central to the eco-friendly treatment of propellant materials.In this study,we isolated a polyurethane(PU)-and PUPB-degrading fungus from soils contaminated with rocket propellant,and the fungus H14 was identified as Fusarium solani(Mart.)Sacc.based on macro-and micro-morphology as well as phylogenetic analyses.The ability of F.solani H14 to degrade PU film and PUPB patches was evaluated via mass loss,scanning electron microscopy(SEM)and enzyme production ability.Mass loss analyses revealed a 25.8%reduction in mass of PU and 1.3%reduction in mass of PUPB after F.solani H14 was incubated with PU and PUPB for 90 days,respectively.We found that F.solani H14 mycelia significantly colonized both PU and PUPB.SEM images showed that the surface of PU films and PUPB patches formed holes,underwent folding and experienced damage as well as irregular fissuring from the erosion of fungal hypha.Moreover,two possible degradative enzymes,lipase and esterase,were produced by F.solani.Our study opens a new avenue of research for eco-friendly treatments of explosive materials and propellants.This paper represents the first article on the degradation of PUPB patches.