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Development of a loop‑mediated isothermal amplification assay for detection of Austropeplea tomentosa from environmental water samples

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Animal Diseases 2023年第1期3卷 35-48页

作者： Lily Tran Vignesh A.Rathinasamy travis beddoe Department of Animal Plant and Soil SciencesSchool of AgricultureBiomedicine and EnvironmentLa Trobe UniversityBundooraVIC 3083Australia Full list of author information is available at the end of the article

Lymnaeid snails are key intermediate hosts for the development and survival of Fasciola spp.,the causative agent of Fascioliasis which are economically important parasites infecting humans and livestock *** current control method for treating Fascioliasis is heavily reliant on anthelmintic drugs,particularly Triclabendazole(TCBZ)which has resulted in drug-resistant parasites and poses significant risk as there are no long-term efficacious alternatives *** control measures at the farm level could include both parasite and snail control will play an important role in Fasciola *** and reduce the reliance on anthelmintic *** of such sustainable control measures requires effective identification of snails on the property however Lymnaeid snails are small and difficult to physically *** identification using an environmental DNA approach is a recent approach in which physically locating snails are not *** tomentosa,is the primary intermediate snail host for *** transmission in South-East Australia and we present an in-field loop-mediated isothermal amplification and water filtering method for the detection of *** eDNA from water samples to improve current surveillance *** methodology is highly sensitive with a detection limit of 5×10^(−6)ng/μL,detected in<20 minutes,with cumulative sample preparation and amplification time under 1 *** proposed workflow could assist in monitoring areas to determine the risk of Fascioliasis infection and implement strategies to manage snail populations to ultimately reduce the risk of infection for humans and livestock.

关键词： Fasciola spp. Snail Molecular detection DNA diagnostics LAMP Environmental sampling eDNA

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Go to the back before going forward:Addressing psychological responses in anterior cruciate ligament reconstruction rehabilitation

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Journal of Sport and Health Science 2024年第5期13卷 666-669页

作者： Terese L.Chmielewski Adam Meierbachtol Rick Aberman travis Gunderson Jonathon Sikorski Kathleen Cummer TRIA Orthopedic Center BloomingtonMN 55431USA Rehabilitation Science Graduate Program Department of Family Medicine and Community HealthMedical SchoolUniversity of MinnesotaMinneapolisMN 55455USA Optum Inc. Clinical OperationsPhysical Health DivisionEden PrairieMN 55344USA Dr.Rick Aberman Consulting MinnetonkaMN 55305USA Department of Rehabilitation Medicine Division of Physical TherapyUniversity of WashingtonSeattleWA 98195USA

Helping athletes return to sports participation is a primary goal of anterior cruciate ligament(ACL)reconstruction *** facilitate the achievement of this goal,decades of research studies have sought to identify knee impairments that reduce knee function as well as interventions to resolve them.1Yet,over the past 10 years,research pertaining to psychological responses(i.e.,cognitions and emotions)after ACL reconstruction has grown exponentially—a phenomenon that can be visualized by entering the search terms"psychological"and"anterior cruciate ligament reconstruction"into the PubMed search engine.

关键词： ligament return psychological

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Oncologic outcomes with and without amniotic membranes in robotic-assisted radical prostatectomy:A propensity score matched analysis

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Asian Journal of Urology 2024年第1期11卷 19-25页

作者： Jonathan Noël Daniel Stirt Marcio Covas Moschovas Sunil Reddy Abdel Rahman Jaber Marco Sandri Seetharam Bhat travis Rogers Subuhee Ahmed Anya Mascarenhas Ela Patel Vipul Patel Advent Health Global Robotics Institute CelebrationFLUSA University of Central Florida College of Medicine OrlandoFLUSA Big and Open Data Innovation Laboratory University of BresciaItaly SUNY Upstate Medical University SyracuseNYUSA Kansas City University College of Medicine Kansas CityMOUSA Harvard University CambridgeMAUSA

Objective:Placement of human placenta derived grafts during robotic-assisted radical prostatectomy(RARP)hastens the return of continence and *** long-term impact on the oncologic outcomes remains to be *** objective was to determine the oncologic outcomes of patients with dehydrated human amnion chorion membrane(dHACM)at RARP compared to a matched ***:In a referral centre,from August 2013 to October 2019,599 patients used dHACM in bilateral nerve-sparing *** excluded patients with less than 12 months follow-up,simple prostatectomy,and unilateral *** with dHACM(amnio group)were 529,and were propensity score matched 1:1 to 2465 patients without dHACM(non-amnio group)and a minimum follow-up of 36 *** the time of RARP,dHACM was placed around the neurovascular bundle in the amnio *** and categorical variables in matched groups was tested by two-sample Kolmogorov-Smirnov test and Fisher's exact test *** measured were biochemical recurrence(BCR),adjuvant and salvage therapy ***:Propensity score matching resulted in two groups of 444 *** incidence functions for BCR did not show a difference between the groups(p=0.3).Patients in the non-amnio group required salvage therapy more frequently than the amnio group,particularly after partial nerve-sparing RARP(6.3%vs.2.3%,p=0.001).Limitations are the absence of prospective ***:The data suggest that using dHACM does not have a negative impact on BCR in *** of cancer specific and overall survival will require follow-up study to increase our understanding of these grafts’impact on prostate cancer biology.

关键词： Prostatectomy Prostatecancer Robotic Oncology Outcome Allograft Biomaterial Dehydrated human amnionchorion membrane

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Domestication-related changes in seed dispersal and pigmentation:Visual selection and functional trait?

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Molecular Plant 2023年第8期16卷 1240-1242页

作者： Petr Smykal travis Parker Department of Botany Palacky University in Olomouc77900 OlomoucCzech Republic Department of Plant Sciences University of CaliforniaDavisDavisCA 95616USA

The development of agriculture is one of the most transformative changes in the history of *** the most common changes occurring during plant domestication are reductions in seed dispersal and changes in *** there are archaeological records of these processes,the advancement of genomics offers a tool to achieve greater insight into the process of converting wild plants into crops(Smýkal et al.,2018).This involved and resulted in a set of specific phenotypic changes referred to collectively as the domestication ***,less obvious domestication-related modifications have also been identified,including changes in plant *** processes are often *** example,among visible changes,selection for visual appearance such as pigmentation is governed by modulation of specific metabolic *** genomic tools,the improvement of analytical methods provides the opportunity to reveal metabolomic changes involved in plant domestication.

关键词： Visual collective crops

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The Impact of Sleep-Wake Behaviour on Tennis Match Performance in Junior State Grade Tennis Players

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Journal of Science in Sport and Exercise 2023年第2期5卷 156-166页

作者： Mitchell Turner Philipp Beranek Ian C.Dunican travis Cruickshank School of Medical and Health Sciences Edith Cowan University270 Joondalup DrivePerthWA 6027Australia Centre for Sleep Science School of Human SciencesThe University of Western AustraliaPerthWA 6009Australia Centre for Precision Health School of Medical and Health SciencesEdith Cowan UniversityPerthAustralia Perron Institute for Neurological and Translational Sciences PerthAustralia

Purpose To date,no study has investigated the extent to which sleep-wake behaviour(SWB)influences match performance in junior tennis *** study aimed to assess the influence of SWB for the week and night before on match performance,particularly match analytics and *** This study recruited 10 junior state grade tennis players who wore an actigraphy device and completed a sleep diary for the week before their match on two separate occasions throughout their competition *** wore a global positioning system device to track their movement during matches,and an experienced tennis coach recorded players'match *** This study showed that the sleep fragmentation index was significantly lower the week before matches in females who had won than those who had ***,the sleep fragmentation index was significantly lower the night before a given match than the week *** sleep fragmentation index and sleep latency significantly influenced match performance in junior tennis *** percentage of second serves points won differed between match wins and losses for male players,while winners and forced errors differed for female *** These findings provide a detailed profile of tennis match play in junior state grade *** individual differences,reduced restlessness the night before a match coincides with increased match performance.

关键词： Sleep fragmentation Tennis analytics Global positioning system Sleep quality Sleep duration

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Development of necrotizing enterocolitis after blood transfusion in very premature neonates

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World Journal of Pediatrics 2023年第1期19卷 68-75页

作者： travis L.Odom Jessica Eubanks Nusiebeh Redpath Erica Davenport Dmitry Tumin Uduak S.Akpan Department of Pediatrics University of Texas Health Sciences Campus7703 Floyd Curl DriveSan AntonioTX 78229-3900USA Department of Pediatrics Brody School of Medicine at EastCarolina UniversityGreenvilleNCUSA ECU Health Medical Center GreenvilleNCUSA

Background Prior studies report conflicting evidence on the association between packed red blood cell(PRBC)transfu-sions and necrotizing enterocolitis(NEC),especially in early weeks of life where transfusions are frequent and spontaneous intestinal perforation can mimic *** primary objective of this study was to evaluate the association between PRBC transfusions and NEC after day of life(DOL)14 in very premature *** A retrospective cohort analysis of very premature neonates was conducted to investigate association between PRBC transfusions and NEC after DOL *** endpoints were PRBC transfusions after DOL 14 until the date of NEC diagnosis,discharge,or *** ranked-sum and Fisher's exact tests,Cox proportional hazards regression,and Kaplan-Meier curves were used to analyze *** Of 549 premature neonates,186(34%)received transfusions after DOL 14 and nine(2%)developed NEC(median DOL=38;interquartile range=32-46).Of the nine with NEC after DOL 14,all were previously transfused(P<0.001);therefore,hazard of NEC could not be *** hoc analysis of patients from DOL 10 onward included five additional patients who developed NEC between DOL 10 and DOL 14,and the hazard of NEC increased by a factor of nearly six after PRBC transfusion(hazard ratio=5.76,95%confidence interval=1.02-32.7;P=0.048).Conclusions Transfusions were strongly associated with NEC after DOL *** studies are needed to determine if restrictive transfusion practices can decrease incidence of NEC after DOL 14.

关键词： Necrotizing enterocolitis Packed red blood cells Transfusions Very low birth weight infants

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Self-gating enhanced carrier transfer in semiconductor electrocatalyst verified in microdevice

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Chinese Chemical Letters 2022年第6期33卷 3221-3226页

作者： Haiyan Xiang Yueshao Zheng Yang Chen Yanting Xu travis Shihao Hu Yexin Feng Yu Zhou Song Liu Xuli Chen State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China Hunan Provincial Key Laboratory of Low-Dimensional Structural Physics and Devices School of Physics and ElectronicsHunan UniversityChangsha 410082China Department of Mechanical Engineering California State UniversityLos AngelesCA 90032United States School of Physics and Electronics Central South UniversityChangsha 410082China College of Materials Science and Engineering Hunan UniversityChangsha 410082China

Semiconductor electrocatalysis with weak conductivity can accumulate extremely high carriers at semiconductor-electrolyte interface by self-gating effect,which strongly promotes electrocatalytic *** correlation between semiconductor carrier mobility and electrocatalysis performance is still *** atomic-thin transition metal dichalcogenides based composites have been developed for hydrogen evolution reaction(HER)performed with on-chip *** and electrochemical measurement of individual flack verified the key role of high carrier mobility for enhanced HER *** mobility regulation further demonstrated its high dependence with HER performance under *** study provides new insight into the carrier mobility of the semiconductor in the electrocatalysis,paving the way for designing high-performance semiconductor catalysts.

关键词： Chemical vapor deposition Pt NPs@MoS_(2) Hydrogen evolution reaction Microelectrochemical Self-gating

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Black soldier fly(Diptera:Stratiomyidae)larval heat generation and management

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Insect Science 2023年第4期30卷 964-974页

作者： Chujun Li Nicola F.Addeo travis W.Rusch Aaron M.Tarone Jeffery K.Tomberlin Department of Entomology Texas A&M UniversityCollege StationTexas State Key Laboratory of Biocontrol School of Life ScienceSun Yat-sen UniversityGuangzhouChina Guangzhou Unique Biotechnology Co. LtdGuangzhouChina Department of Veterinary Medicine and Animal Production University of Napoli Federico llNapoliItaly

Mass production of black soldier fly,Hermetia illucens(L.)(Diptera:Stra-tiomyidae),larvae results in massive heat generation,which impacts facility management,waste conversion,and larval *** tested daily substrate temperatures with dif-ferent population densities(i.e.,0,500,1000,5000,and 10000 larvae/pan),different pop-ulation sizes(i.e.,166,1000,and 10000 larvae at a fixed feed ratio)and air temperatures(i.e.,20 and 30℃)on various production *** of shifting larvae from 30 to 20℃on either day 9 or 11 were also *** activity increased substrate tem-peratures significantly(i.e.,at least 10℃above air temperatures).Low air temperature favored growth with the higher population sizes while high temperature favored growth with low population *** greatest average individual larval weights(e.g.,0.126 and 0.124 g)and feed conversion ratios(e.g.,1.92 and 2.08 g/g)were recorded for either 10000 larvae reared at 20℃or 100 larvae reared at 30 *** temperatures from high(30℃)to low(20℃)in between(~10-d-old larvae)impacted larval production weights(16%increases)and feed conversion ratios(increased 14%).Facilities should consider the impact of larval density,population size,and air temperature during black soldier fly mass production as these factors impact overall larval production.

关键词： Allee effect density Hermetia illucens industry substrate heat thermal ecology

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Mixed-Vehicular Aggregated Transportation Network Design Considering En-route Recharge Service Provision for Electric Vehicles

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Journal of Systems Science & Complexity 2018年第5期31卷 1329-1349页

作者： ZHANG Xiang WALLER S travis School of Civil and Environmental Engineering University of New South Wales Australia

This paper addresses the transportation network design problem (NDP) wherein the distance limit and en-route recharge of electric vehicles are taken into account. Specifically, in this work, the network design problem aims to select the optimal planning policy from a set of infrastructure design scenarios considering both road expansions and charging station allocations under a specified construction budget. The user-equilibrium mixed-vehicular traffic assignment problem with en-route recharge (MVTAP-ER) is formulated into a novel convex optimization model and extended to a newly developed bi-level program of the aggregated NDP integrating recharge facility allocation (NDP-RFA). In the algorithmic framework, a convex optimization technique and a tailored GA are adopted for, respectively, solving the subproblem MVTAP-ER and the primal problem NDP-RFA. Systematic experiments are conducted to test the efficacy of the proposed approaches. The results highlight the impacts of distance limits and budget levels on the project selection and evaluation, and the benefits of considering both road improvement policy and recharge service provision as compared to accounting for the latter only. The results also report that the two design objectives, to respectively minimize the total system travel time and vehicle miles travelled, are conflicting for certain scenarios.

关键词：混合车辆网络设计交通网络线路聚集服务设计问题旅行时间

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Tudor and its domains: germ cell formation from a Tudor perspective

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Cell Research 2005年第4期15卷 281-291页

作者： travis THOMSON,Paul LASKO Canada H3A 1B1 Department of Biology McGill University Montreal Quebec Canada H3A 1B1 Department of Biology McGill University Montreal Quebec

In many metazoan species, germ cell formation requires the germ plasm, a specialized cytoplasm which often con-tains electron dense structures. Genes required for germ cell formation in Drosophila have been isolated predominantlyin screens for maternal-effect mutations. One such gene is tudor (tud); without proper tud function germ cell formationdoes not occur. Unlike other genes involved in Drosophila germ cell specification tud is dispensable for other somaticfunctions such as abdominal patterning. It is not known how TUD contributes at a molecular level to germ cell forma-tion but in tud mutants, polar granule formation is severely compromised, and mitochondrially encoded ribosomal RNAsdo not localize to the polar granule. TUD is composed of 11 repeats of the protein motif called the Tudor domain. Thereare similar proteins to TUD in the germ line of other metazoan species including mice. Probable vertebrate orthologuesof Drosophila genes involved in germ cell specification will be discussed.

关键词： Drosophila tudor germ cells germ plasm Tudor domains.

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