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A Review of Skin Banking Guidelines and Standards Worldwide:Towards the Harmonization of Guidelines for Skin Banking in Therapeutic Applications for the Regions under the Asia Pacific Burn Association(APBA)

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Burns & Trauma 2020年第1期8卷 82-93页

作者： Wee Ling Heng Qi Wei Wang Renuka Sornarajah Jacynthe Tremblay Nandita Melati Putri Suzina Sheikh Ab Hamid Pornthep Pungrasmi Hsian-Jenn Wang Dong Chul Kim daizo Saito Nhu lam Nguyen Wan Azman Wan Sulaiman Aditya Wardhana Vinita Puri Hajime Matsumura Niann-Tzyy dai Rajeev BAhuja Gaoxing Luo Weifeng He Si Jack Chong Alvin Wen Choong Chua transplant Tissue Centre Singhealth Duke-NUS Transplant CentreSingapore Heart Valve and Tissue Bank Lady Ridgeway Hospital for ChildrenDr Danister De Silva MawathaColomboSri Lanka Jacynthe Tremblay Consultation QuebecCanada Burn Unit Faculty of MedicineCipto Mangunkusumo General HospitalJakartaIndonesia Otorhinolaryngology-Head and Neck Surgery School of Medical SciencesHealth CampusUniversiti Sains MalaysiaKelantanMalaysia Plastic and Reconstructive Surgery Division King Chulalongkorn Memorial HospitalBangkokThailand Plastic and Reconstructive Surgery Taipei Medical UniversityTaipeiChinese Taipei Plastic and Reconstructive Surgery Bundang Jesaeng HospitalSeongnamSouth Korea Division of Traumatology Research InstituteNational Defence Medical CollegeSaitamaJapan Vietnam National Burn Hospital HanoiVietnam Reconstructive Sciences Programme School of Medical SciencesHealth CampusUniversiti Sains MalaysiaKelantanMalaysia Department of Plastic Surgery King Edward Memorial Hospital and Seth Gordhandas Sunderdas Medical CollegeMumbaiIndia Department of Plastic and Reconstructive Surgery Tokyo Medical UniversityTokyoJapan Division of Plastic and Reconstructive Surgery Department of SurgeryTri-Service General HospitalNational Defense Medical CenterTaipei Plastic and Aesthetic Surgery Sir Ganga Ram HospitalNew DelhiIndia Institute of Burn Research Southwest HospitalArmy Medical UniversityChongqingChina Department of Plastic Reconstructive and Aesthetic SurgerySingapore General HospitalSingapore Musculoskeletal Sciences Academic Clinical Programme Duke-NUS Medical SchoolSingapore

Currently,there are no harmonized guidelines which govern skin banking in the Asia Pacific ***,skin banks are either unregulated or rely on their nation’s legislation or international accreditation to uphold their quality standards.A new set of skin banking guidelines was devel-oped through a comprehensive review and collation of best international practices for the Asia Pacific Burn Association(APBA)members,from donor screening and testing,to skin recovery,processing,storage and distribution,and quality *** regulatory requirements reviewed include the European directives,Australia’s Therapeutic Goods Administration and Sin-gapore’s tissue banking *** technical and quality management recommendations are referenced from the American Association of Tissue Banks(AATB),the United States Food and Drug Administration standards and guidance documents,various relevant European guides,Japanese Society of Tissue transplantation guidelines and the Asia Pacific Association of Surgical Tissue *** mainly from the AATB standards,the new Asia Pacific Burn Association Guidelines for Skin Banking in Therapeutic Applications offer a comprehensive manual,address-ing:governance and contracts;staff responsibilities;quality management;facilities,equipment and supplies management;donor consent and testing;and recommendations of good practices pertaining to skin recovery,processing,storage and *** complementing current generic regulations,they provide technical specifications of major aspects unaddressed in most *** inaugural set of new regional skin banking guidelines would be a start for regional members of the APBA to adopt,and will hopefully culminate in a set of standards so that,in the long run,skin allografts from this region can be of similar quality,which can simplify import process and facilitate the exchange of allografts between members.

关键词： Skin banking Skin allografts Skin transplantation Tissue banking Tissue donation

Investigation of Two Precipitation Methods for Extracting Immunoglobulin Y(IgY)from Egg Yolks

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Veterinary Science Research 2021年第2期3卷 23-30页

作者： tran Thi Quynh Lan tran Trong Kha Faculty of Animal Science and Veterinary Medicine Nong Lam University-HCM city6th QuarterLinh Trung WardThu Duc DistrictHo Chi Minh cityVietnam

Two groups of hens(control and immunization group)were arranged in an experimental design with an immunization schedule of 3 injections of BSA *** antibodies were extracted from egg yolks by two precipitation processes(chloroform and polyethylene glycol precipitates)and quantified using a standard curve of protein *** purification of IgY was confirmed by *** protein extracted from egg yoks were less contaminated with yellow pigments(lutein and zeaxanthin)nd by using chloroform *** 2 week post-immunization,IgY-1 concentration increased respectively to 3903±726μ***(chloroform-1 extraction process)and 2937±294μ***(PEG extraction process)(P

关键词： Immunoglobulin Y Egg yolk Precipitation Polyethylene glycol Chloroform

Strigolactones interact with other phytohormones to modulate plant root growth and development

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固态发酵酶制剂充分释放水产饲料中潜在的营养物质

The Crop Journal 2022年第6期

作者： Huwei Sun Weiqiang Li David J.Burritt Hongtao Tian Heng Zhang Xiaohan Liang Yuchen Miao Mohammad Golam Mostofa lam-Son Phan tran

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饲料广角 2009年第1期 46-48页

作者： Le Thanh Huang tran Ngoc Thien Kim Alison Leary 越南Nong lam大学水产系奥特奇亚太生物技术研究中心

1 前言亚太地区的水产养殖业面临许多挑战．饲料是人们关注的重点之一。饲料原料价格上涨．某些原料供应短缺以及质量降低已导致饲料原料市场的动荡和变化不定：植物蛋白原料已不再是笃定的饲料蛋白来源：生物燃料的乙醇工业与动物养殖... 详细信息

1 前言亚太地区的水产养殖业面临许多挑战．饲料是人们关注的重点之一。饲料原料价格上涨．某些原料供应短缺以及质量降低已导致饲料原料市场的动荡和变化不定：植物蛋白原料已不再是笃定的饲料蛋白来源：生物燃料的乙醇工业与动物养殖业竞争原料．已大大改变了饲料原料的成本结构。寻求可替代的饲料原料．饲料利用最佳化和动物生产最佳化的技术．以及减少营养物质排泄和减少饲料浪费的技术在当今的水产养殖和水产饲料配方中起着越来越重要的作用。

关键词：水产饲料营养物质固态发酵酶制剂水产养殖业饲料原料动物养殖业亚太地区

The characteristics of codon usage pattern in hepatitis B virus with rtM204V/I mutation by pyrosequencing

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The characteristics of codon usage pattern in hepatitis B vi...

应用因子分析模型评价白酒行业上市公司财务竞争力

中华医学会第十六次全国病毒性肝炎及肝病学术会议

作者： Wei Hou Chenyang dai Hangyu Pei Wukui Cao Yuqiang Mi Jiming Yang Wei Lu Tianjin Second Peoples Hospital and Tianjin Institute of Hepatology

Background/aims The aim of this study was to investigate the characteristics of tyrosine-methionine-aspartate -aspartate(YMDD) mutation and analyze the codon usage pattern of YMDD variants in patients with lamivudine(lam) -resistant chronic hepatitis B(CHB).Methods 514 CHB inpatients and outpatients from our hospital with confirmed genotypic resistance to lam were enrolled in this study between 2008 and 2012.

关键词：

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酿酒科技 2013年第7期 117-120页

作者：黄妍戴新民安徽工业大学研究生院安徽马鞍山243000

为提高白酒企业的财务竞争力,通过定性分析与定量分析相结合,以及整体分析和专项分析相结合,应用SPSS19.0统计软件分别对白酒行业18家上市公司从盈利能力、偿债能力、营运能力、成长能力4个方面的13项财务指标进行处理,建立了一套较为... 详细信息

为提高白酒企业的财务竞争力,通过定性分析与定量分析相结合,以及整体分析和专项分析相结合,应用SPSS19.0统计软件分别对白酒行业18家上市公司从盈利能力、偿债能力、营运能力、成长能力4个方面的13项财务指标进行处理,建立了一套较为科学的企业财务竞争力评价体系,并依据所建模型对典型企业的财务竞争力进行实证分析,提出提升企业财务竞争力的可行性措施。

关键词：白酒行业财务竞争力 SPSS 因子分析财务评价

慢性乙型肝炎拉米夫定治疗后病毒基因变异的分析

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中国现代医学杂志 2010年第21期20卷 3350-3353页

作者：刘伟李代红天津市第一中心医院输血科天津300192

目的探讨拉米夫定治疗慢性乙型肝炎发生病毒变异的情况,从而有效地指导临床治疗和预后判断。方法采用基因测序技术检测74例经拉米夫定治疗后发生病毒变异的血清进行乙肝病毒P区全序列测定,按不同变异位点分为4组(rtV173L、rtL180M、rtM2... 详细信息

目的探讨拉米夫定治疗慢性乙型肝炎发生病毒变异的情况,从而有效地指导临床治疗和预后判断。方法采用基因测序技术检测74例经拉米夫定治疗后发生病毒变异的血清进行乙肝病毒P区全序列测定,按不同变异位点分为4组(rtV173L、rtL180M、rtM204V/I、rtL180M与rtM204V/I联合组),比较4个位点变异患者用药前及发生变异后的ALT、HBV DNA含量的变化情况。结果 74例拉米夫定变异患者rtV173L发生变异4例,rtL180M发生变异13例,rtM204V/I发生变异22例,rtL180M与rtM204V/I联合组发生变异35例。服药前各组ALT、HBV DNA含量差异无显著性(P>0.05),rtL180M与rtM204V/I联合组发生变异的患者ALT和HBV DNA含量较单一变异组高,差异有显著性(P<0.05)。结论乙型肝炎拉米夫定治疗发生变异位点为rtV173L、rtL180M、rtM204V/I、rtL180M与rtM204V/I联合组。联合变异组各项生化指标以及HBV DNA含量变化明显,提示临床预后较差。

关键词：乙型肝炎拉米夫定基因变异

PSA及NCAM表达差异COS-7细胞的建立及PSA对COS-7细胞迁移侵袭能力的影响

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中国药理学通报 2007年第12期23卷 1565-1570页

作者：程亚彬李静戴功耿美玉中国海洋大学医药学院分子药理室山东青岛266003

目的构建PSA与NCAM表达差异的COS-7细胞,并检测PSA的表达对细胞粘附、迁移侵袭能力的影响,为今后进一步研究PSA介导的细胞迁移侵袭信号通路变化,阐明PSA对肿瘤转移影响的分子机制奠定基础。方法该文通过双转染质粒构建PSA与NCAM表达差异... 详细信息

目的构建PSA与NCAM表达差异的COS-7细胞,并检测PSA的表达对细胞粘附、迁移侵袭能力的影响,为今后进一步研究PSA介导的细胞迁移侵袭信号通路变化,阐明PSA对肿瘤转移影响的分子机制奠定基础。方法该文通过双转染质粒构建PSA与NCAM表达差异的COS-7细胞株,利用流式细胞术及Western blot方法检验转染效率;采用MTT法检测PSA表达差异COS-7细胞的增殖;粘附分析法检测COS-7细胞对细胞外基质Fn的粘附能力;利用tran-swell小室比较PSA与NCAM表达差异的COS-7细胞迁移、侵袭能力。结果PSA与NCAM表达差异COS-7细胞构建成功,PSA可降低COS-7细胞与基底膜的粘附,增强其迁移及侵袭能力。结论该文所建立的PSA与NCAM表达差异的COS-7细胞株可用于今后进行PSA对细胞迁移侵袭信号通路影响的机制研究。

关键词： PSA NCAM COS-7 迁移侵袭肿瘤

DPHL:A DIA Pan-human Protein Mass Spectrometry Library for Robust Biomarker Discovery

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Genomics, Proteomics & Bioinformatics 2020年第2期18卷 104-119页

作者： Tiansheng Zhu Yi Zhu Yue Xuan Huanhuan Gao Xue Cai Sander R.Piersma Thang V.Pham Tim Schelfhorst Richard R.G.D.Haas Irene V.Bijnsdorp Rui Sun Liang Yue Guan Ruan Qiushi Zhang Mo Hu Yue Zhou Winan J.Van Houdt Tessa Y.S.Le Large Jacqueline Cloos Anna Wojtuszkiewicz Danijela Koppers-Lalic Franziska Bottger Chantal Scheepbouwer Ruud H.Brakenhoff Geert J.L.H.van Leenders Jan N.M.Ijzermans John W.M.Martens Renske D.M.Steenbergen Nicole C.Grieken Sathiyamoorthy Selvarajan Sangeeta Mantoo Sze S.Lee Serene J.Y.Yeow Syed M.F.Alkaff Nan Xiang Yaoting Sun Xiao Yi Shaozheng dai Wei Liu Tian Lu Zhicheng Wu Xiao Liang Man Wang Yingkuan Shao Xi Zheng Kailun Xu Qin Yang Yifan Meng Cong Lu Jiang Zhu Jin'e Zheng Bo Wang Sai Lou Yibei dai Chao Xu Chenhuan Yu Huazhong Ying Tony K.Lim Jianmin Wu Xiaofei Gao Zhongzhi Luan Xiaodong Teng Peng Wu Shi'ang Huang Zhihua Tao Narayanan G.Iyer Shuigeng Zhou Wenguang Shao Henry lam Ding Ma Jiafu Ji Oi L.Kon Shu Zheng Ruedi Aebersold Connie R.Jimenez Tiannan Guo Zhejiang Provincial Laboratory of Life Sciences and Biomedicine Westlake UniversityHangzhou 310024China Key Laboratory of Structural Biology of Zhejiang Province School of Life SciencesWestlake UniversityHangzhou 310024China Institute of Basic Medical Sciences Westlake Institute for Advanced StudyHangzhou 310024China School of Computer Science Shanghai Key Laboratory of Intelligent Information ProcessingFudan UniversityShanghai 200433China Thermo Fisher Scientific(BREMEN)GmbH Bremen 28195Germany OncoProteomics Laboratory Department of Medical OncologyVU University Medical CenterVU UniversityAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamUrologyCancer Center AmsterdamAmsterdam 1011The Netherlands Thermo Fisher Scientific Shanghai 201206China The Netherlands Cancer Institute Surgical OncologyAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamSurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamPediatric Oncology/HematologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamHematologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamMedical OncologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamNeurosurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamPathologyCancer Center AmsterdamAmsterdam 1011The Netherlands Amsterdam UMC Vrije Universiteit AmsterdamOtolaryngology/Head and Neck SurgeryCancer Center AmsterdamAmsterdam 1011The Netherlands Erasmus MC University Medical Center PathologyRotterdam 1016LVThe Netherlands Erasmus MC University Medical Center SurgeryRotterdam 1016LVThe Netherlands Erasmus MC University Medical Center Medical OncologyRotterdam 1016LVThe Netherlands Department of Anatomical Pathology Singapore General HospitalSingapore 169608Singapore Division of Medical Scie

To address the increasing need for detecting and validating protein biomarkers in clinical specimens,mass spectrometry(MS)-based targeted proteomic techniques,including the selected reaction monitoring(SRM),parallel reaction monitoring(PRM),and massively parallel dataindependent acquisition(DIA),have been *** optimal performance,they require the fragment ion spectra of targeted peptides as prior *** this report,we describe a MS pipeline and spectral resource to support targeted proteomics studies for human tissue *** build the spectral resource,we integrated common open-source MS computational tools to assemble a freely accessible computational workflow based on *** then applied the workflow to generate DPHL,a comprehensive DIA pan-human library,from 1096 data-dependent acquisition(DDA)MS raw files for 16 types of cancer *** extensive spectral resource was then applied to a proteomic study of 17 prostate cancer(PCa)***,PRM validation was applied to a larger study of 57 PCa patients and the differential expression of three proteins in prostate tumor was *** a second application,the DPHL spectral resource was applied to a study consisting of plasma samples from 19 diffuse large B cell lymphoma(DLBCL)patients and 18 healthy control *** expressed proteins between DLBCL patients and healthy control subjects were detected by DIA-MS and confirmed by *** data demonstrate that the DPHL supports DIA and PRM MS pipelines for robust protein biomarker *** is freely accessible at https://***/page/***?id=IPX0001400000.

关键词： Data-independent acquisition Parallel reaction monitoring Spectral library Prostate cancer Diffuse large B cell lymphoma