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Improved resolution of mixed STR profiles using a fully automated differential cell lysis/DNA extraction method

Forensic Sciences Research 2020年第2期5卷 106-112页

作者： Matthew C.Goldstein Jordan O.Cox Lori B.Seman tracey dawson cruz Department of Forensic Science Virginia Commonwealth UniversityRichmondVirginiaUSA

Sexual assault evidence often contains sperm cells,which are typically separated from nonsperm cells using manual differential lysis *** goal of this study was to evaluate the automated QIAGEN QIAcube for this purpose and to compare it to manual QIAGEN and manual organic differential methods using DNA yields and STR profile data for *** yields were determined by qPCR,followed by multiplex STR amplification,CE analysis,and mixture *** automated method was capable of effective cell separation,producing DNA yields sufficient for STR ***,sperm fraction human:male DNA ratios from the QIAcube samples were consistently closer to the desired 1:1 and STR profiles were less likely to result in mixtures,with 6–8fewer female alleles detected(median 1.5 alleles).Ultimately,using the QIAcube for automated differential processing of semen-containing mixtures reduces the need for downstream mixture interpretation and improves STR profile quality with substantially less hands-on time.

关键词： Forensic sciences forensic genetics sexual assault evidence QIAcube differential lysis sperm lysis automated DNA extraction mixture analysis

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Effects of storage time on DNA profiling success from archived latent fingerprint samples using an optimised workflow

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Forensic Sciences Research 2022年第1期7卷 61-68页

作者： Sydney I.Menchhoff April D.Solomon Jordan O.Cox Madison E.Hytinen Marilyn T.Miller tracey dawson cruz Department of Forensic Science Virginia Commonwealth UniversityRichmondVirginiaUSA Jefferson Parish Sheriff’s Office Regional DNA Laboratory HarveyLouisianaUSA

Due to recent improvements in forensic DNA testing kit sensitivity,there has been an increased demand in the criminal justice community to revisit past convictions or cold *** of these cases have little biological evidence other than touch DNA in the form of archived latent fingerprint lift *** this study,a previously developed optimised workflow for this sample type was tested on aged fingerprints to determine if improved short tandem repeat(STR)profiles could be ***-year-old samples processed with the optimised workflow produced an average of approximately five more STR alleles per profile over the traditional *** optimised workflow also produced detectable alleles in samples aged out to 28 *** the methods tested,the optimised workflow resulted in the most informative profiles from evidence samples more representative of the forensic *** workflow is recommended for use with archived latent fingerprint samples,regardless of the archival time.

关键词： Forensic sciences forensic genetics low template DNA touch DNA aged samples archived latent fingerprints optimised workflow

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Land use and dingo baiting are correlated with the density of kangaroos in rangeland systems

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Integrative Zoology 2023年第2期18卷 299-315页

作者： Stuart J.dawson tracey L.KREPLINS Malcolm S.KENNEDY Juanita RENWICK Mark A.COWAN Patricia A.FLEMING Terrestrial Ecosystem Science and Sustainability Harry Butler InstituteMurdoch UniversityMurdochAustralia Department of Primary Industries and Regional Development(present address) South PerthAustralia Department of Primary Industries and Regional Development NorthamAustralia Queensland Department of Environment and Science Australia Queensland Department of Environment and Science MoggillAustralia Department of Biodiversity Conservation and AttractionsWoodvaleAustralia

Rangelands worldwide have been subject to broadscale modiﬁcation,such as widespread predator control,intro-duction of permanent livestock water and altered vegetation to improve *** Australia,these landscape changes have resulted in kangaroos(*** macropods)populations increasing over the past 200 ***-garoos are a key contributor to total grazing pressure and in conjunction with livestock and feral herbivores have been linked to land *** used 22 years of aerial survey data to investigate whether the density of 3 macropod species in the southern rangelands of Western Australia was associated with:(i)land use,including type of livestock,total livestock,density of feral goats,type of land tenure,and kangaroo commercial harvest effort;(ii)predator management,including permitted dingo control effort,estimated dingo abundance,and presence of the State Barrier Fence(a dingo exclusion fence);and(iii)environmental variables:ruggedness,rainfall,fractional cover,and total standing dry *** kangaroos(Osphranter rufus)were most abundant inﬂat,open vegetation,on pastoral land,where area permitted for dingo control was high,and numbers were positively associated with antecedent rainfall with a 12-month *** grey kangaroos(Macropus fuliginosus)were most abundant onﬂat,agricultural land,but less abundant in areas with high permitted dingo ***(Osphranter robustus)were most abundant in rugged pastoral land with open vegetation,where permitted dingo control was *** environmental variables are key drivers of landscape productivity and kangaroo populations,anthropogenic factors such as land use and permitted dingo control are strongly associated with kangaroo abundance.

关键词： herbivores livestock macropods overgrazing rangelands total grazing pressure

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