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Study on Distribution of Four Pseudomonas Species in Living Environment Using Multiplex PCR

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Open Journal of Stomatology 2024年第2期14卷 77-86页

作者： Sachiyo Hayashi Koji umezawa Osamu Tsuzukibashi Akira Fukatsu Mana Fuchigami Chiaki Komine Hiroshi Yamamoto Mio Hagiwara-Hamano Yukiko Iizuka Satoshi Uchibori Masanobu Wakami Hiroshi Murakami Taira Kobayashi Masahiko Fukumoto Takato Nomoto Department of Special Needs Dentistry Nihon University School of Dentistry at Matsudo Chiba Japan Department of Laboratory Medicine for Dentistry for the Compromised Patient Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Surgery Nihon University School of Dentistry at Matsudo Chiba Japan Department of Fixed Prosthodontics Nihon University School of Dentistry at Matsudo Chiba Japan Department of Oral Implantology Nihon University School of Dentistry at Matsudo Chiba Japan

Purpose: The genus Pseudomonas is a ubiquitous microorganism frequently detected from immunocompromised patients. The inherent resistance to numerous antimicrobial agents contributes to the opportunistic character of this pathogen exhaustive monitoring of this pathogen is considered of critical importance to public health organizations. The reliable identification method able to distinguish genetic close Pseudomonas species is needed, because these organisms are difficult to differentiate by phenotypic or biochemical methods. The purpose of the present study was to design species-specific primers in order to identify and detect four Pseudomonas species which are frequently detected from the human oral cavities, and to investigate the distribution of these organisms in the living environment using a multiplex PCR. Methods: Polymerase chain reaction (PCR) primers were designed based on partial sequences of the rpoD gene of four Pseudomonas species. Swab samples were collected from fifty washstands, and the distribution of Pseudomonas species was investigated using a conventional PCR at genus level and a multiplex PCR at species level. Results: Multiplex PCR method developed in this study was able to distinguish four Pseudomonas species clearly. The genus Pseudomonas was detected from all samples (100%), whereas P. putida, P, aeruginosa, P. stutzeri and P. fluorescens were detected at 44%, 8%, 4% and 2% in fifty swab samples, respectively. Conclusion: Our developed one-step multiplex PCR method is accurate, specific, cost-effective, time-saving, and works without requiring DNA extraction. It was indicated that washstands were the uninhabitable environment for P. putida, P, aeruginosa, P. stutzeri and P. fluorescens.

关键词： Genus Pseudomonas Multiplex PCR Living Environment

Woody plant cell walls:Fundamentals and utilization

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Molecular Plant 2024年第1期17卷 112-140页

作者： Wei Li Ying-Chung Jimmy Lin Ying-Lan Chen Chenguang Zhou Shuang Li Nette De Ridder Dyoni M.Oliveira Lanjun Zhange Baocai Zhang Jack P.Wang Changzheng Xu Xiaokang Fu Keming Luo Ai-Min Wu Taku Demura Meng-Zhu Lu Yihua Zhou Laigeng Li Toshiaki umezawa Wout Boerjan Vincent L.Chiang State Key Laboratory of Tree Genetics and Breeding Northeast Forestry UniversityHarbin 150040China Department of Life Science National Taiwan UniversityTaipeiTaiwanChina Department of Biotechnology and Bioindustry Sciences College of Bioscience and BiotechnologyNational Cheng Kung UniversityTainanTaiwanChina Department of Plant Biotechnology and Bioinformatics Ghent UniversityTechnologiepark 719052 GhentBelgium VIB Center for Plant Systems Biology Technologiepark 719052 GhentBelgium State Key Laboratory of Plant Genomics Insttute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Forest Biotechnology Group Department of Forestry and Environmental ResourcesNorth Carolina State UniversityRaleighNC 27695USA Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation Integrative Science Center of Germplasm CreationSchool of Life SciencesSouthwest UniversityChongqing 400715China Guangdong Key Laboratory for Innovative Developmentand Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitecturesSouth China Agricultural UniversityGuangzhou510642China Center for Digital Green-innovation Nara Institute of Science and TechnologykomaNara 630-0192Japan State Key Laboratory of Subtropical Siviculture College of Forestry and BiotechnologyZhejang A&F UniversityHangzhou 311300China CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences300 Fenglin RoadShanghai 200032China Laboratoryof MetabolicScienceofForest Plants and Microorganisms Rsearch Institute for Sustainable HumanosphereKyoto UniversityUjiKyoto 611-0011 Japan

Cell walls in plants,particularly forest trees,are the major carbon sink of the terrestrial *** and biosynthetic features of plant cell walls were revealed early on,focusing mostly on herbaceous model *** developments in genomics,transcriptomics,epigenomics,transgenesis,and associated analytical techniques are enabling novel insights into formation of woody cell ***,we review multilevel regulation of cell wall biosynthesis in forest tree *** highlight current approaches to engineering cell walls as potential feedstock for materials and energy and survey reported field tests of such engineered transgenic *** outline opportunities and challenges in future research to better understand cell type biogenesis for more efficient wood cell wall modification and utilization for biomaterials or for enhanced carbon capture and storage.

关键词： regulation of cell wall formation biosynthesis of cell wall components lignin cellulose hemicellulose cell wall utilization

A regression model-based method for indoor positioning with compound location fingerprints

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Geo-Spatial Information Science 2019年第2期22卷 107-113,I0003页

作者： Tomofumi Takayama Takeshi umezawa Nobuyoshi Komuro Noritaka Osawa Hewlett-Packard Japan LtdTokyoJapan Graduate School of Engineering Chiba UniversityChibaJapan

This paper proposed and evaluated an estimation method for indoor *** method combines location fingerprinting and dead reckoning differently from the conventional *** uses compound location fingerprints,which are composed of radio fingerprints at multiple points of time,that is,at multiple positions,and displacements between them estimated by dead *** avoid errors accumulated from dead reckoning,the method uses short-range dead *** method was evaluated using 16 Bluetooth beacons installed in a student room with the dimensions of 11×5 m with furniture *** Received Signal Strength Indicator(RSSI)values of the beacons were collected at 30 measuring points,which were points at the intersections on a 1×1 m grid with no obstacles.A compound location fingerprint is composed of RSSI vectors at two points and a displacement vector between *** Forests(RF)was used to build regression models to estimate positions from location *** root mean square error of position estimation was 0.87 m using 16 Bluetooth *** error is lower than that received with a single-point baseline model,where a feature vector is composed of only RSSI values at one *** results suggest that the proposed method is effective for indoor positioning.

关键词： Indoor positioning integrated estimation radio fingerprinting dead reckoning machine learning non-linear regression

Optimization of fuel consumption and NO_(x) emission for mild HEV via hierarchical model predictive control

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Control Theory and Technology 2022年第2期20卷 221-234页

作者： Yuka umezawa Ken Yamauchi Hiroki Seto Toshiro Imamura Toru Namerikawa Graduate School of Science and Technology Keio University3-14-1 HiyoshiKohoku-ku.YokohamaKanagawa 223-8522Japan Isuzu Advanced Engineering Center Ltd 8 TsuchidanaFujisawa.Kanagawa 252-0881.Japan

In this paper,we consider the fuel economy optimization problem for a mild hybrid electric vehicle(HEV)using hierarchical model predictive *** the proposed algorithm,two problems are addressed:eco-driving and torque *** the eco-driving problem,vehicle speed was *** the reduction in fuel consumption and NOx emissions,the torque required to follow the target speed was ***,in the torque distribution problem,the distribution between the engine and motor torques were *** this phase,engine characteristics were *** problems differ in terms of time scales;therefore,a hierarchical model predictive control is ***,the numerical simulation results demonstrated the efficacy of this research.

关键词： Mild HEV Energy management Model predictive control Hierarchical control

A visual analytics system to support the formation of a hypothesis from calcium wave data

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Visual Informatics 2018年第1期2卷 2-13页

作者： Kozen umezawa Hiroaki Natsukawa Yosuke Onoue Koji Koyamadab Department of Electrical Engineering Graduate School of EngineeringKyoto UniversityKyotoJapan Academic Center for Computing and Media Studies Kyoto UniversityKyotoJapan Advanced Research Center for the Promotion of Interdisciplinary Education and Research Kyoto UniversityKyotoJapan

In most species,calcium waves in the oocyte are considered common phenomena in the activation of ***,the mechanism of calcium waves has not yet been *** collaborating with biologists studying Caenorhabditis elegans(***),which is widely used as a model organism,we observed that the following requirements must be satisfied to form a useful hypothesis based on calcium waves captured using high-speed in vivo imaging:(1)the ability to obtain an overview of how the calcium waves are propagated and(2)the ability to understand the propagation of waves in a narrow ***,conventional visualization methods cannot satisfy these requirements ***,we propose a visual analytics system that allows users to understand and explore calcium wave images using cross-correlation analysis of the time-series data of the Ca^(2+)fluorescence intensity at each *** interface of this system comprises an overview visualization,a detail visualization,and user interactions to satisfy these requirements and realize exploratory *** views present an overview visualization that displays the clustering results of a directed graph calculated using cross-correlation *** views enable the users to understand the overview of wave propagation,thereby helping users find a region of *** detail visualization shows the relationship between the region of interest and other ***,users can use the proposed system with overview-detail and brush-link exploration to assign meaning to the region of interest and construct a hypothesis for its *** this paper,we demonstrate how the proposed visual analytics approach works and how new hypotheses can be formed using the analysis of *** calcium waves.

关键词： Visual Knowledge Discovery Time Series Data Bioinfonnatics Visualization

Phosphorylation of LTF1, an MYB Transcription Factor in Populus, Acts as a Sensory Switch Regulating Lignin Biosynthesis in Wood Cells

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Molecular Plant 2019年第10期12卷 1325-1337页

作者： Jinshan Gui Laifu Luo Yu Zhong Jiayan Sun Toshiaki umezawa Laigeng Li National Key Laboratory of Plant Molecular Genetics and CAS Center for Excellence in Molecular Plant Sciences Shanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of the Chinese Academy of Sciences Beijing 100049China School of Life Science Lanzhou UniversityLanzhou 730000China Research Institute for Sustainable Humanosphere Kyoto UniversityUjiKyoto 611-0011Japan

Lignin is specifically deposited in plant secondary cell walls,and initiation of lignin biosynthesis is regulated by a variety of developmental and environmental ***,the mechanisms governing the regulation of lignin biosynthesis remain to be *** this study,we identified a lignin biosynthesis-associated transcription factor(LTF)from Populus,LTF1,which binds the promoter of a key lignin biosynthetic gene encoding 4-coumarate-CoA ligase(4CL).We showed that LTF1 in its unphosphorylated state functions as a regulator restraining lignin *** LTF1 becomes phosphorylated by PdMPK6 in response to external stimuli such as wounding,it undergoes degradation through a proteasome pathway,resulting in activation of *** of a phosphorylation-null mutant version of LTF1 led to stable protein accumulation and persistent attenuation of lignification in wood *** together,our study reveals a mechanism whereby LTF1 phosphorylation acts as a sensory switch to regulate lignin biosynthesis in response to environmental *** discovery of novel modulators and mechanisms modifying lignin biosynthesis has important implications for improving the utilization of cell-wall biomass.

关键词： lignin Populus secondary xylem cell wall

Engineered three-dimensional rabbit oral epithelial–mesenchymal–muscular hybrid sheets

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International Journal of Oral Science 2016年第3期8卷 145-154页

作者： Shigeki Yamane Kazunari Higa Takashi umezawa Masamitsu Serikawa Jun Shimazaki Shinichi Abe Department of Anatomy Tokyo Dental College Tokyo Japan Department of Ophthalmology/Cornea Center Tokyo Dental College Chiba Japan

Regenerative muscles are required for swallowing and mastication, and are important for functional recovery from diseases involving oral muscular defects. Therefore, we generated three-layer hybrid sheets, similar to oral mucosal structures containing submucosal muscles, using rabbit oral mucosa epithelial, mesenchymal, and myoblastic progenitor cells, and examined the structural proteins. Each cell type was obtained from rabbit oral mucosa using enzymatic digestion. Isolated mesenchymal and myoblastic cells were multi-differentiated into osteoblasts, adipocytes, and chondrocytes or myotubes. Isolated epithelial cells were cultured on collagen gels containing isolated mesenchymal cells for 2 weeks, and these epithelial-mesenchymal cell sheets were laminated onto myoblastic cell sheets. The engineered hybrid sheets were multi-stratified in the epithelial and myoblastic layers in a time-dependent manner, expressing intermediate cytoskeletal filament proteins of epithelium and muscle. Hybrid sheets also expressed extracellular matrix basement membrane proteins. Immature cell markers for epithelial and myoblastic cells were observed continuously in hybrid sheet cultures. We established engineered three-dimensional rabbit oral mucosa hybrid sheets containing each immature cell type in vitro.

关键词： mesenchymal stem cell multi-differentiation myoblast oral mucosa three-dimensional culture

Favorable response after radiation therapy for intraductal papillary mucinous neoplasms manifesting as acute recurrent pancreatitis:A case report

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World Journal of Clinical Cases 2022年第30期10卷 11116-11121页

作者： Ayaka Harigai Kiyoshi Kume Noriyoshi Takahashi So Omata Rei umezawa Keiichi Jingu Atsushi Masamune Department of Radiation Oncology Tohoku University Graduate School of MedicineSendai 980-8574Japan Division of Gastroenterology Tohoku University Graduate School of MedicineSendai 980-8574Japan

BACKGROUND There has been an increasing number of elderly patients with intraductal papillary mucinous neoplasm(IPMN),who are surgically intolerant and require less invasive treatment options,which are *** the present study,we report a case of IPMN presenting with acute recurrent pancreatitis(ARP),in which radiation therapy effectively prevented further attacks of ARP and reduced tumor *** SUMMARY An 83-year-old man was referred to our hospital with an asymptomatic incidental pancreatic *** ultrasound imaging and magnetic resonance cholangiopancreatography revealed a multiloculated tumor in the head of the pancreas,with dilated pancreatic ducts and mural *** patient was diagnosed with mixed-type IPMN,and five years later,he developed *** endoscopic pancreatic ductal balloon dilatations failed to prevent further ARP *** was considered clinically inappropriate because of his old age and *** was referred to our department for radiation therapy targeted at those lesions causing intraductal hypertension and radiation was administered at a dose of 50 *** magnetic resonance imaging scan taken ten weeks after treatment revealed a decrease in tumor size and improvement of pancreatic duct *** months later,he remains symptom-free from *** This case highlights the important role of radiation therapy in mitigating the signs and symptoms of ARP in patients with inoperable IPMN.

关键词： Intraductal papillary mucinous neoplasm Acute recurrent pancreatitis Pancreas Radiation therapy Case report

Zinc-Based Metal-Organic Frameworks for High-Performance Supercapacitor Electrodes:Mechanism Underlying Pore Generation

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Energy & Environmental Materials 2023年第2期6卷 100-112页

作者： Shigeyuki umezawa Takashi Douura Koji Yoshikawa Daisuke Tanaka Vlad Stolojan S.Ravi P.Silva Mika Yoneda Kazuma Gotoh Yasuhiko Hayashi Seiwa Electric Mfg.Co.Ltd. Kyoto 610-0192Japan Graduate School of Natural Science and Technology Okayama UniversityOkayama 700-8350Japan School of Science and Technology Kwansei Gakuin UniversityHyogo 669-1337Japan JST PRESTO2-1 GakuenHyogo 669-1337Japan Advanced Technology Institute University of SurreyGuilfordSurreyGU27XHUK

Heat treatment of metal-organic frameworks(MOFs)has provided a wide variety of functional carbons coordinated with metal *** this study,two kinds of zinc-based MOF(ZMOF),C_(16)H_(10)O_(4)Zn(ZMOF1)and C_(8)H_(4)O_(4)Zn(ZMOF2),were ***1 and ZMOF2 were carbonized at 1000℃,forming CZMOF1 and CZMOF2,*** specific surface area(S_(BET))of CZMOF2 was~2700 m^(2)g^(−1),much higher than that of CZMOF1(~1300 m^(2)g^(−1)).A supercapacitor electrode based on CZMOF2 achieved specific capacitances of 360,278,and 221 F g^(−1)at 50,250,and 1000 mA g^(−1)in an aqueous electrolyte(H2SO_(4)),respectively,the highest values reported to date for ZMOF-derived electrodes under identical *** practical applicability of the CZMOF-based supercapacitor was verified in non-aqueous *** initial capacitance retention was 78%after 100000 charge/discharge cycles at 10 A g^(−1).Crucially,the high capacitance of CZMOF2 arises from pore generation during *** 1000℃,pore generation is dominated by the Zn/C ratio of ZMOFs,as carbon atoms reduce the zinc oxides formed during *** 1000℃,a high O/C ratio becomes essential for pore generation because the oxygen functional groups are *** findings will provide insightful information for other metal-based MOFderived multifunctional carbons.

关键词： metal-organic frameworks pore generation porous carbons supercapacitor zinc oxides