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生物质资源与工程(英文) 2023年第3期8卷 224-234页

作者： Peifu Kong Junichi Peter Abe Shunsuke Masuo toshiharu enomae Degree Programs in Life and Earth Sciences University of TsukubaIbaraki 305-8572Japan Faculty of Life and Environmental Sciences University of TsukubaIbaraki305-8572Japan

This study aimed to prepare tea tree oil-β-cyclodextrin microcapsules using an optimized co-precipitated method.The impact of the volume fraction of ethanol in the solvent system for microencapsulation on encapsulation efficiency was investigated and analyzed sophisticatedly.Super-high encapsulation efficiency was achieved when a 40%volume fraction of ethanol was used for the microencapsulation procedure,where the recovery yield of microcapsules and the embedding fraction of tea tree oil in microcapsules were as high as 88.3%and 94.3%,respec-tively.Additionally,considering the operation cost,including time and energy consumption,an economical preparation was validated so that it would be viable for large-scale production.Based on the results of morphological and X-ray diffraction analysis,the crystal structure appeared to differ before and after microencapsulation.The results of gas chromatography-mass spectrometry and Fourier transform infrared spectroscopy confirmed the successful formation of microcapsules.Furthermore,the antibacterial activity of the fabricated microcapsules was assessed by a simple growth inhibition test using Bacillus subtilis as the study object,and the hydrophilic property was proved by a water contact angle measurement.

关键词： β-cyclodextrin Co-precipitation Encapsulation efficiency Microcapsule Tea tree oil

Bioinspired cellulose-based membranes in oily wastewater treatment

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Frontiers of Environmental Science & Engineering 2022年第7期16卷 139-172页

作者： Abdul Halim Lusi Ernawati Maya Ismayati Fahimah Martak toshiharu enomae Department of Chemical Engineering Universitas Internasional Semen IndonesiaGresik61122Indonesia Department of Chemical Engineering Kalimantan Institute of TechnologyBalikpapan76127Indonesia Research Center for Biomaterials National Research and Innovation Agency(BRIN)Bogor16911Indonesia Department of Chemistry Faculty of SciencesSepuluh Nopember Institute of TechnologyKampus ITS SukoliloSurabaya60111Indonesia Faculty of Life and Environmental Sciences University of TsukubaTsukuba305-8572Japan

It is challenging to purify oily wastewater, which affects water-energy-food production. One promising method is membrane-based separation. This paper reviews the current research trend of applying cellulose as a membrane material that mimics one of three typical biostructures: superhydrophobic, underwater superoleophobic, and Janus surfaces. Nature has provided efficient and effective structures through the evolutionary process. This has inspired many researchers to create technologies that mimic nature’s structures or the fabrication process. Lotus leaves, fish scales, and Namib beetles are three representative structures with distinct functional and surface properties: superhydrophobic, underwater superoleophobic, and Janus surfaces. The characteristics of these structures have been widely studied and applied to membrane materials to improve their performance. One attractive membrane material is cellulose, whichhas been studied from the perspective of its biodegradability and sustainability. In this review, the principles, mechanisms, fabrication processes, and membrane performances are summarized and compared. The theory of wettability is also described to build a comprehensive understanding of the concept. Finally, future outlook is discussed to challenge the gap between laboratory and industrial applications.

关键词： Cellulose Bioinspired membrane Superhydrophobic surface Underwater superoleophobic surface Oil-water separation

Joint input–output identiﬁcation of unstable systems with kernel regularization

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Control Theory and Technology 2024年第2期22卷 195-202页

作者： Yusuke Fujimoto toshiharu Sugie Department of Environmental Engineering the University of Kitakyushu1-1HibikinoWakamatsu-kuKitakyushu 8080135FukuokaJapan Professor Emeritus Kyoto UniversityYoshida-HonmachiSakyo-kuKyoto 6068501KyotoJapan

This paper discusses closed-loop identiﬁcation of unstable systems.In particular,weﬁrst apply the joint input–output identiﬁcation method and then convert the identiﬁcation problem of unstable systems into that of stable systems,which can be tackled by using kernel-based regularization methods.We propose to identify two transfer functions by kernel regularization,the one from the reference signal to the input,and the one from the reference signal to the output.Since these transfer functions are stable,kernel regularization methods can construct their accurate models.Then the model of unstable system is constructed by ratio of these functions.The effectiveness of the proposed method is demonstrated by a numerical example and a practical experiment with a DC motor.

关键词： Closed-loop identification Kernel regularization Joint input-output identification

FIBER ORIENTATION DISTRIBUTION OF PAPER SURFACE CALCULATED BY IMAGE ANALYSIS

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天津科技大学学报 2004年第A2期19卷 51-57页

作者： toshiharu enomae Yoon-Hee Han Akira Isogai Assistant Professor Paper Science Laboratory Department of Biomaterial SciencesGraduate School of Agricultural and Life SciencesThe University of TokyoJAPANPh.D. Candidate Paper Science Laboratory Department of Biomaterial SciencesGraduate School of Agricultural and Life SciencesThe University of TokyoJAPANProfessor Paper Science Laboratory Department of Biomaterial SciencesGraduate School of Agricultural and Life SciencesThe University of TokyoJAPAN

Anisotropy of paper is an important parameter of paper structure. Image analysis technique was improved for accurate fiber orientation in paper surfaces. Image analysis using Fast Fourier Transform was demonstrated to be an effective means to determine fiber orientation angle and its intensity. Binarization process of micrograph images of paper surface and precise calculation for average Fourier coefficients as an angular distribution by interpolation developed were found to improve the accuracy. This analysis method was applied to digital optical micrographs and scanning electron micrographs of paper. A laboratory handsheet showed a large deviation in the average value of fiber orientation angle, but some kinds of machine-made paper showed about 90 degrees in the orientation angle with very small deviations as expected. Korea and Japanese paper made in the traditional ways showed its own characteristic depending on its hand making processes.

关键词：各向异性方向性分布图象分析快速傅立叶变换方向性强度日本纸韩国纸纸浆纤维

The supercomplex formation between the chloroplast NADH dehydrogenase-like complex and photosystem Ⅰ

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Molecular Plant 2022年第4期15卷 589-590页

作者： toshiharu Shikanai Graduate School of Science Kyoto UniversityKyotoJapan

The chloroplast genomes of land plants encode 11 genes(ndhA-ndhK)for proteins similar to the subunits of respiratory complex I(NADH dehydrogenase)with some exceptions.It was a mystery why the chloroplast genomes encode the genes related to respiratory electron transport.We understand now that the genes encode the subunits of the chloroplast NADH dehydrogenaselike(NDH)complex mediating the antimycin A-resistant pathway of cyclic electron transport around photosystem I(Peltier et al.,2016).This year,cryo-EM images of the NDH complex associated with two copies of photosystem I(PSI)complex(NDH-PSI supercomplex)from barley(Shen et al.,2022)and Arabidopsis(Su et al.,2022)were published.The chloroplast NDH complex may not be the complex enigma in both physiological function and structure.Here I briefly summarize what is clear and what is left unanswered focusing on the structural aspect.

关键词： structure. NADH complex

A review of isolated muscular ventricular septal defect

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World Journal of Pediatrics 2020年第2期16卷 120-128页

作者： toshiharu Miyake Department of Pediatrics Kindai University Nara Hospital1248-1OtodaIkomaNara 630-0293Japan

Background Color Doppler echocardiography greatly facilitates the diagnosis of isolated muscular ventricular septal defect with a small shunt.Data sources Original research articles were collected from database,including PubMed and Google scholar.Relevant articles about muscular ventricular septal defect were included.Results The frequency of isolated muscular ventricular septal defect is 5.7%in preterm infants and 1.1-5.3%in term infants.Spontaneous closure in muscular ventricular septal defect occurs with higher frequency and earlier than in perimembranous ventricular septal defect.Approximately 80-90%of isolated muscular ventricular septal defect closes spontaneously by 12 months of age.Midventricular muscular ventricular septal defect is spontaneously closed earlier in the short term,but no site difference is found in the long term.The spontaneous closure mechanism is regarded as aposition of the muscle tissueor fibrous tissue formation in the right ventricular side,but in rare cases involves aneurysm formation of the fibrous tissue.Regarding spontaneous closure of isolated muscular ventricular septal defect diagnosed for the fetus,further studies are needed.Chromosomal microarray analysis of fetuses with isolated muscular ventricular septal defect has revealed that it is not a severe risk factor of chromosomal abnormalities.Conclusions This paper presents a review of the history of the diagnosis and frequency of ventricular septal defect,with discussion of its natural history from the fetal period to after birth in patients with isolated muscular ventricular septal defect.

关键词： Color Doppler echocardiography Fetus Infant Spontaneous closure

Contribution of Cyclic and Pseudo-cyclic Electron Transport to the Formation of Proton Motive Force in Chloroplasts

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Molecular Plant 2017年第1期10卷 20-29页

作者： toshiharu Shikanai Hiroshi Yamamoto Department of Botany Graduate School of Science Kyoto University Oiwake-cho Kitashirakawa Sakyo-ku Kyoto 606-8502 Japan CREST Japan Science and Technology Agency Chiyoda-ku Tokyo 102-0076 Japan

Photosynthetic electron transport is coupled to proton translocation across the thylakoid membrane, re- sulting in the formation of a trans-thylakoid proton gradient （△pH） and membrane potential （△ψ）. Ion trans-porters and channels localized to the thylakoid membrane regulate the contribution of each component to the proton motive force （pmf）. Although both △pH and △ψ contribute to ATP synthesis as pmf, only ~pH downregulates photosynthetic electron transport via the acidification of the thylakoid lumen by inducing thermal dissipation of excessive absorbed light energy from photosystem II antennae and slowing down of the electron transport through the cytochrome bsf complex. To optimize the tradeoff between efficient light energy utilization and protection of both photosystems against photodamage, plants have to regulate the pmf amplitude and its components, △pH and △ψ. Cyclic electron transport around photosystem I （PSI） is a major regulator of the pmf amplitude by generating pmf independently of the net production of NADPH by linear electron transport. Chloroplast ATP synthase relaxes pmf for ATP synthesis, and its activity should be finely tuned for maintaining the size of the pmf during steady-state photosynthesis. Pseudo-cyclic electron transport mediated by flavodiiron protein （FIv） forms a large electron sink, which is essential for PSI photoprotection in fluctuating light in cyanobacteria. FIv is conserved from cyanobacteria to gymno- sperms but not in angiosperms. The Arabidopsis proton gradient regulation 50（pgr5） mutant is defective in the main pathway of PSI cyclic electron transport. By introducing Physcomitrella patens genes encoding Flvs, the function of PSI cyclic electron transport was substituted by that of FIv-dependent pseudo-cyclic electron transport. In transgenic plants, the size of the pmf was complemented to the wild-type level but the contribution of △pH to the total pmf was lower than that in the wild type. In the pgr5 mutant, the size of the pmf was drastically lowered by the absence o

关键词： alternative electron transport cyclic electron transport around photosystem I ion channel flavodiironprotein proton motive force pseudo-cyclic electron transport

Sewerage Force Adjustment Technology for Energy Conservation in Vacuum Sanitation Systems

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Chinese Journal of Mechanical Engineering 2013年第2期26卷 334-340页

作者： GUO Zhonghua LI Xiaoning KAGAWA toshiharu School of Mechanical Engineering Nanjing University of Science and Technology Precision and Intelligence Laboratory Tokyo Institute of Technology

The vacuum sanitation is the safe and sound disposal approach of human excreta under the specific environments like flights, high speed trains and submarines. However, the propulsive force of current systems is not adjustable and the energy consumption does not adapt to the real time sewerage requirement. Therefore, it is important to study the sewerage force adjustment to improve the energy efficiency. This paper proposes an energy conservation design in vacuum sanitation systems with pneumatic ejector circuits. The sewerage force is controlled by changing the systematic vacuum degree according to the amount of the excreta. In particular, the amount of the excreta is tested by liquid level sensor and mass sensor. According to the amount of the excreta, the relationship between the excreta amount and the sewerage force is studied to provide proper propulsive force. In the other aspect, to provide variable vacuum degrees for different sanitation requirements, the suction and discharge system is designed with pneumatic vacuum ejector. On the basis of the static flow-rate characteristics and the vacuum generation model, the pressure response in the ejector circuit is studied by using the static flow rate characteristics of the ejector and air status equation. The relationship is obtained between supplied compressed air and systematic vacuum degree. When the compressed air is supplied to the ejector continuously, the systematic vacuum degree increases until the vacuum degree reaches the extreme value. Therefore, the variable systematic vacuum degree is obtained by controlling the compressed air supply of the ejector. To verify the effect of energy conservation, experiments are carried out in the artificial excreta collection, and the variable vacuum-degree design saves more than 30% of the energy supply. The energy conservation is realized effectively in the new vacuum sanitation systems with good application prospect. The proposed technology provides technological support for the energy conservation of vacuum sanita

关键词： energy conservation vacuum sanitation system pneumatic ejector circuit vacuum degree

Promising anticancer therapy: combination of immune checkpoint inhibitors and molecular-targeted agents

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Hepatobiliary Surgery and Nutrition 2020年第6期9卷 777-779页

作者： toshiharu Sakurai Naoshi Nishida Masatoshi Kudo Department of Gastroenterology and Hepatology Kindai University Faculty of MedicineOsakaJapan

Hepatocellular carcinoma(HCC)is the fourth leading cause of cancer death worldwide,and prognosis remains unsatisfactory when the disease is diagnosed at an advanced stage.Sorafenib resistance is one of the main obstacles to the treatment of advanced HCC.Several studies have proposed that the activation of escape pathways from RAF/MEK/ERK/STAT3 might result in sorafenib resistance(1-3).Leung et al.showed that HCC cells with higher SHP2 expression was associated with a lower sensitivity towards sorafenib treatment(4).Consistently,SHP2 inhibitor SHP099 abrogated sorafenib resistance in cancer cells by blocking RAS/MEK/ERK and AKT signaling pathways(4).Unlike other tyrosine phosphatases,SHP2 functions as a positive regulator of proliferative signals.

关键词： STAT3 death treatment

Scaling relation of domain competition on(2+1)-dimensional ballistic deposition model with surface diffusion

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Journal of Semiconductors 2016年第9期37卷 12-17页

作者： Kenyu Osada Hiroyasu Katsuno toshiharu Irisawa Yukio Saito Department of Physics Gakushuin University Computer Centre Gakushuin University Department of Physical Science Ritsumeikan University Department of Physics Keio University

During heteroepitaxial overlayer growth multiple crystal domains nucleated on a substrate surface compete with each other in such a manner that a domain covered by neighboring ones stops growing.The number density of active domains ρ decreases as the height h increases.A simple scaling argument leads to a scaling law of ρ~ h^（-γ） with a coarsening exponent γ=d/z,where d is the dimension of the substrate surface and z the dynamic exponent of a growth front.This scaling relation is confirmed by performing kinetic Monte Carlo simulations of the ballistic deposition model on a two-dimensional（d=2） surface,even when an isolated deposited particle diffuses on a crystal surface.

关键词： domain competition ballistic deposition model Kardar-Parisi-Zhang universality class surface diffusion