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Effect of acupuncture at acupoint of Shenshu (BL 23) on the bones of ovariectomized rats

Journal of Traditional Chinese Medicine 2018年第1期38卷 54-60页

作者： Atsushi Takeda Teruhiko Koike Shigeru Urata torao ishida Yoshiharu Oshida School of Medicine Nagoya Uni- versity Showa-ku 466-8550 Japan Department of Acupuncture Medicine Suzuka University of Medical Science Suzuka 510-0293 Japan Research Center of Health Physical Fitness and Sports Nagoya University Fu- rou-cho Chikusa-ku Nagoya 464-8601 Japan Institute of Traditional Chinese Medicine Suzuka University of Medical Science Suzuka 510-0226 Japan

OBJECTIVE： To investigate the effect of acupuncture at acupoint of Shenshu （BL 23） in ovariectomized rats and sham-operated rats. METHODS： Female Sprague-Dawley rats were divided into the following four groups （n = 12 each）： sham operation only （Sham）, sham operation with acupuncture （Sham ＋ ACP）, ovariectomy only （OVX）, and ovariectomy with acupuncture （OVX ＋ ACP）. Operations were performed at the 9th week of age. Acupuncture of Shenshu （BL 23） was started at the 1 lth week of age and conducted 3 times per week until the 26th week of age. RESULTS： Among ovariectomized rats, the acupuncture-treated rats had significantly lower body weights and cross-linked N-telopeptide of type 1 collagen levels in the urine after 3 weeks of acu- puncture treatment, higher bone mineral density of the lumbar vertebra after 7 weeks, and smaller trabecular separation of the sixth lumbar vertebra, measured by micro-computed tomography, after 17 weeks than control rats. These effects were not observed in sham-operated rats. Blood concentrations of estradiol were significantly higher in the OVX ＋ ACP group than in the untreated group. Acupuncture treatment increased plasma testosterone levels in sham-operated rats, while this increase was not observed in OVX rats. CONCLUSION： Acupuncture of Shenshu （BL 23） prevents bone loss and structural changes by sup- pressing bone resorption.

关键词： Acupuncture Point BL23 (Shenshu) Es- trogens Osteoporosis Ovariectomy

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An Ageusia and Dysosmia Patient Treated by Acupuncture

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Chinese Journal of Integrated Traditional and Western Medicine 2006年第1期12卷 66-67页

作者：张文平 torao ishida Institute of Traditional Chinese Medicine Suzuka University of Medical Science Mie Japan

Occurrence of ageusia, a disease characterized by complete or severe loss of the subjective sense of taste and frequently accompanied with olfaction disorders, is increasing at the rate of 140 thousand more cases every year in Japan according to the latest congressional data, 2002. There may be many reasons for this, such as influenza, diabetes, adverse reaction of antimicrobial drugs, smoke, depression, etc. Disorders of taste and smell could not only cause uncomfort, but also result in a loss of body weight in the patients, and may be harmful to their quality of life.

关键词： An Ageusia and Dysosmia Patient Treated by Acupuncture

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Different frequencies of electroacupuncture and semen coicis decrease glial fibrillary acidic protein expression in rats with hemisection spinal cord injury

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Neural Regeneration Research 2011年第24期6卷 1870-1875页

作者： Chunguang Hou Zhongzheng Li Yi Guo Yongming Guo Yangyang Liu Chao Wang ishida torao Experimental Acupuncture Research Center Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Oriental Medicine Institute of Suzuka Medical Science College Kishiokachou Suzuka 1001-1 Japan

This study established the following groups of rats： a normal group, a sham surgery group, a spinal cord injury model group, a low-frequency electroacupuncture group, a high-frequency electroacupuncture group and a semen coicis group. In all but the normal and sham surgery groups the left half of Tlo was transected. Four hours after model induction, 5-Hz and 100-Hz electroacupuncture were used to stimulate the acupoints I-luantiao （GB 30）, Zusan/i （ST 36）, Zhiyan9 （DU 9） and Xuanshu （DU 5）, or crude extract from semen coicis was intraperitoneally injected, for 8 consecutive weeks. The results indicated that electroacupuncture stimulation and intraperitoneal injection of semen coicis improved the morphology of spinal cord tissue, promoted the recovery of motion-evoked potentials, suppressed glial fibrillary acidic protein expression, and ameliorated motor function in rats with hemisection spinal cord injury. The effects of high-frequency （100 Hz） electroacupuncture ancl semen coicis were significant.

关键词： electroacupuncture semen coicis hemisection spinal cord injury traditional Chinese medicine neural regeneration

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Effects of Fuscoporia obliqua on Postprandial Glucose Excursion and Endothelial Dysfunction in Type 2 Diabetic Patients

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Journal of Traditional Chinese Medicine 2008年第1期28卷 49-57页

作者： Toshihiro Maenaka Masami Oshima Yuka Itokawa Takashi Masubuchi Yasuyuki Takagi Jung-Sook Choi torao ishida Yeunhwa Gu Graduate School of Health Science Suzuka University of Medical ScienceJapan Gyeongdo provincial college Kyoungbook Korea Hi-tech Research Center Suzuka University of Medical Science Japan Graduate School of Health Science Suzuka University of Medical Science Japan Hi-tech Research Center Suzuka University of Medical Science Japan

Postprandial hyperglycemia has been reported to elicit endothelial dysfunction and provoke future cardiovascular complications. A reduction of postprandial blood glucose levels by the glucosidase inhibitor Fuscoporia obliqua was associated with a risk reduction of cardiovascular complications, but the effects of Fuscoporia obliqua on endothelial function have never been elucidated. This study is aimed to assess the efficacy of Fuscoporia obliqua on postprandial metabolic parameters and endothelial function in type 2 diabetic patients. Postprandial peak glucose (14.47±1.27 vs. 8.50±0.53 mmol/liter), plasma glucose excursion (PPGE), and change in the area under the curve (AUC) glucose after a single loading of test meal (total 450 kcal; protein 15.3%; fat 32.3%; carbohydrate 51.4%) were significantly higher in the diet-treated type 2 diabetic patients (n=14) than the age-and sex-matched controls (n=12). The peak forearm blood flow response and total reactive hyperemic flow (flow debt repayment) during reactive hyperemia, indices of resistance artery endothelial function on strain-gauge plethysmography, were unchanged before and after meal loading in the controls. But those of the diabetics were significantly decreased 120 and 240 min after the test meal. A prior administration of Fuscoporia obliqua decreased postprandial peak glucose, PPGE, and AUC glucose. The peak forearm blood flow and flow debt repayment were inversely well correlated with peak glucose, PPGE, and AUC glucose, but not with AUC insulin or the other lipid parameters. Even a single loading of the test meal was shown to impair the endothelial function in type 2 diabetic patients, and the postprandial endothelial dysfunction was improved by a prior use of Fuscoporia obliqua. Fuscoporia obliqua might reduce macrovascular complication by avoiding endothelial injury in postprandial hyperglycemic status.

关键词： Fuscoporia oblique Glucose excursion and Endothelial Dysfunction Type 2 Diabetic Patients Insulin

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A Trial Study of Propranolol and Zhigancao Decoction on the Central Depressant and Anti-osteoporosic Action in Ovariectomized Rats

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Journal of Traditional Chinese Medicine 2008年第1期28卷 64-70页

作者： Zhang Wenping Masayuki Kanehara Zhang Yanjun Guo Yi Shigeru Urata Hiroyuki Nakazawa Takayasu Murakami Atushi Takeda torao ishida Acupuncture Department Suzuka University of Medical Science 1001-1 Kishioka Suzuka Mie 510-0293 Japan Tianjin University of Traditional Chinese Medicine Tianjin China.

Objective： This study was originally designed to observe the effects of propranolol （a β-blocker） and Zhigancao Decoction （炙甘草汤 ZGCD） on bone mass in ovariectomized rats. Methods： Thirty-eight female Sprague-Dawley rats were divided into four groups initially, a sham-operated group （Sham, n=7）, a model ovariectomized （OVX） group （Model, n=7）, a propranolol group （Pro, n=12） and a ZGCD group （ZGCD, n=12）. After 15 weeks of treatment, the expected effects were not found. In order to verify the situations of the experiment, we modified the study by administering calcitonin to a subgroup of the tested Pro and ZGCD rats. Results： The Pro and ZGCD treatments showed decreased heart rate and plasma norepinephrine level, but neither an increased bone mass nor any bone metabolism differences from the model rats were found. However, the OVX-induced bone loss was prevented by the sequent treatment of calcitonin. Conclusions： The results provide no evidence that the β-blocker propranolol may stimulate bone formation, and do not iustify its use for clinical treatment of osteoporosis.

关键词： propranolol Zhigancao Decoction bone sympathetic nerve

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Effects of Propolis on Peripheral White Cells, Antioxidative Activity and Tumor Growth in Irradiated mice

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Journal of Traditional Chinese Medicine 2006年第4期26卷 299-305页

作者： Takashi Nakamura Yuka Itokawa Kwang-Ho Cho Jung-Sook Choi Ikukatsu Suzuki Toshihoro Miura torao ishida Yeunhwa Gu Graduate School of Health Science Suzuka University of Medical Science Japan Gyeongdo provincial college Kyoungbook korea Department of Radiological Science Catholic University of Deagu College of Health Science Deagu Korea Hi-tech Research Center Suzuka University of Medical Science Japan

The effect of continuous water-soluble propolis administration on radioactivity-induced reduction of hemocytes, and the antioxidant and antitumor effects were investigated. Following a 1-week adjustment period, water-soluble propolis was administered intraperitoneally to male ICR mice at a dose of 100 mg/kg every other day for 2 weeks. Following administration, 2 Gy whole-body irradiation was performed and the counts of leukocytes, lymphocytes, and granulocytes and monocytes in the peripheral blood were determined 1, 3, 7, 15 and 30 days after irradiation. In the second experiment, water-soluble propolis was similarly administered to the mice for 2 weeks after a 1-week adjustment period, and 2 Gy whole-body irradiation was performed. The antioxidant effects in hemocytes were then investigated using 2,2＇-azobis（2-amidinopropane） dihydrochloride （AAPH）, a radical generator. In the third experiment, 1×10^6 Sarcoma-180 cells were inoculated into the right thigh of mice, which were divided into four groups： control, water-soluble propolis-treated, 6 Gy irradiated and water-soluble propolis-treated ＋ 6 Gy irradiated groups, and changes in tumor size were measured for 20 days. Results show that administration of water-soluble propolis inhibits the reduction of hemocytes caused by whole-bodv irradiation, enhances antioxidant effects against radioactivity, and inhibits tumor growth.

关键词： Propolis Antioxidant effects radioprotective effects Anti-tumor T lymphocyte

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Radioprotective Effect of Lyophyllum Decastes and the Effect on Immunological Functions in Irradiated Mice

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Journal of Traditional Chinese Medicine 2007年第1期27卷 70-75页

作者： Takashi Nakamura Yuka Itokawa Masayuki Tajima Yuuichi Ukawa Kwang-Ho Cho Jung-Sook Choi torao ishida Yeunhwa Gu Graduate School of Health Science Suzuka University of Medical Science Oji Forest & Product Co. Ltd Department of Radiological Science Catholic University of Deagu College of Heallth Science Gyeongdo Provincial College Hi-tech Research Center Suzuka University of Medical Science

In this study, to explore the radiation protection effects of Lyophyllum Decastes Sing （LDS）, a hot distilled-water extract of LDS was orally administered at a dosage of 250mg/kg every other day for a period of 2 weeks in irradiated mice. An automatic blood cell counter was used to measure white blood cells （lymphocytes, monocyte, and granulocytes） one day before X-ray irradiation, and 3 hours, 12 hours, 24 hours, 3 days, 7 days, 15 days and 30 days after irradiation. The Dunnett test was used to examine statistical significance of differences. The peripheral blood cell counts in the Lyophyllum-administered non-irradiation group revealed an increase in the numbers of ieukocytes, lymphocytes and monocytes. For 2 Gy whole body radiation, a significant statistical difference was found between the X-ray group and the Lyophyllum plus X-ray group in the numbers of leukocytes, lymphocytes and monocytes. The results suggest that Lyophyllum restrains blood cell-count falling after irradiation, which is probably mediated at least in part by hemopoietic function, and NK and LAK activities seems to play a role in preventing secondary irffections associated with irradiation.

关键词： Lyophyllum Decastes (LD) Radiation Lymphocytes NK LAK

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Effects of K. Lysolecithin on Blood Levels of Monoamines in Mice

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Journal of Traditional Chinese Medicine 2007年第3期27卷 212-219页

作者： Yuka Itokwa Aki Kozu Takashi Nakamura Takashi Masubuchi Masami Oshima Kwang-Ho Cho Jung-Sook Choi Toshihiro Miura torao ishida Yeunhwa Gu Suzuka Univercity of Medical Science Graduate School of Health Science Society of Preventive and Alternative Medicine Department of Radiological Science Catholic University of Deagu College of Health Science Gyeongdo Provincial College

In this research, Lysolecithin-a substance made with 100% natural ingredients - was given to ICR mice as medication to measure its periodic effect on the noradrenalin (NA), dopamine (DA), and serotonin (5-HT) levels of the brain. Both ICR and SAM mice were separated into two groups - control group and Lysolecithin (K. Lysolecithin: hydrolytic lysolecithin) medicated group, and given 1-week preparation period. The K. Lysolecithin group was given 500mg/kg of K. Lysolecithin at 0.2mL per dosage for 4 weeks, and the control group was given the same amount of dosage of water during the same period. NA, DA and 5-HT concentrations were measured from the blood before medication and 8 weeks / 12 weeks / 16 weeks after the first medication. For the SAM mice, 8 weeks after they were medicated with K .Lysolecithin, Morris Water Maze Test was conducted for 7 consecutive days and then the concentrations were measured by drawing blood from the heart. The K. Lysolecithin medicated group showed a tendency to have a statistically significant higher concentrations of 5-HT and NA in the blood. Also, periodic examination showed that the monoamine levels were highest in the 12th week and declined thereafter.

关键词： K. Lysolecithin Serotonin Noradrenalin Dopamine Alzheimer's Syndrome

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The More Efficacious Acupoints of Zusanli and Sanyinjiao Than That of Non-acupoints on Bone Mass in Osteopenic Ovariectomized Rats

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Chinese Journal of Integrated Traditional and Western Medicine 2005年第3期11卷 209-216页

作者：张文平 KANEHARA Masayuki 张艳军于志锋张国霞杨幼新孙玉敏张建梅 ishida torao Institute of Traditional Chinese Medicine Suzuka University of Medical Science Tianjin College of Traditional Chinese Medicine

Objective： To clarify whether the acupoints of Zusanli （ST36） and Sanyinjiao （SP6） have specific actions other than non-acupoints to bone. Methods： Forty Sprague-Dawley female rats were divided into five groups： Sham operated （sham） group; Ovariectomized （OVX, model） group; non-acupuncture group; OVX, needling on Zusanli and Sanyinjiao （Acp-A） group; OVX, needling on the reverse sides of Zusanli and Sanyinjiao （Acp-B） group; OVX, periostineal stimulation on the same height as points of Zusanli and Sanyinjiao （Acp-C） group. The experiment was continued for 23 weeks and then all animals were sacrificied. Results： OVX had a significantly higher body weight and lower bone mineral density （BMD） on the lumbar vertebrae, total femora and tibiae than sham rats, however, Acp-A showed a higher BMD compared with the other OVX groups. On the other hand, bone weights, bone strength and bone morphometry such as trabecular volume, trabecular separation, labeled width and bone formation rate also showed the same improvements in Acp-A as compared to the other OVX rats. Conclusion： The stimulation on Zusanli and Sanyinjiao specifically prevented the development of osteopenic rats compared with non-acupoints.

关键词： acupuncture osteopenia ovariectomy bone mineral density histomorphometry

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Effects of Chinese Herbal Medicine Based on Hachimi-jio-gan on Osteopenia in Rats

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Journal of Traditional Chinese Medicine 2006年第1期26卷 72-77页

作者： Masayuki Kanehara Takeshi Ogirima Kaori Tano Toshihiro Maenaka torao ishida Institute of Traditional Chinese Medicine and Graduate School of Health Science Suzuka University of Medical Science1001-1 Kishioka SuzukaMie Pref.510-0293Japan Institute of Traditional Chinese Medicine and Graduate School of Health Science Suzuka University of Medical Science1001-1KishiokaSuzukaMie Pref.510-0293Japan

The effects of Chinese herbal medicines including Hachimi-jio-gan(HJG)and/or Hochu-ekki-to(HET)on osteopenia inrats were *** Chinese herbal medicines were administrated for 8 weeks(7 times/week)starling from 1 weekafter *** and Prescription-2(Prsc-2,the prescription based on HJG)showed protective effect on bone loss ofthe vertebrae after ***,Prescription-1(the prescription based on HJG and HE-T)had no ***,wemade osteopenia model in rats by prednisolone and low calcium ***-2 and HJG were administrated for 20 weeks *** Chinese remedies showed protective effects for osteopenia,with better indices on bone loss of the limbsthan HJG alone in the osteopenia *** can be concluded that Prsc-2 is more effective than HJG for bone loss induced byvarious factors,and the additives in Prsc-2 may enhance the therapeutic effect.

关键词：中医药疗法骨质减少小鼠动物实验

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