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Journal of Huazhong University of Science and Technology(Medical Sciences) 1999年第3期19卷 219-222页

作者： XIA Qinghua ) 1, NIE Haiyang 2, CHEN Daoda, CHEN Jianying, TIAN Yuan, ZHANG Jinhui 1 Department of General Surgery, Xiehe hospital, tongji Medical University, Wuhan 430022 2 Department of Orthopedics, Puqi Peoples hospital, Puqi 437300 Department of General Surgery Xiehe Hospital Tongji Medical University Wuhan 430022

A new targeting anticancer system was prepared by using hydroxyapatite particles (2 mm in diameter) as carrier material and adriamycin as anticancer agent. The adsorption and release properties of the complexes were assayed by fluorometry in vivo and in vitro and the curative effect on W 256 sarcoma of rat was observed. The results showed that one particle of hydroxyapatite could adsorb approximately 0.08 mg adriamycin and they can maintain a steady and slow release of adriamycin from hydroxyapatite for one month. When hydroxyapatite adriamycin complexes were implanted into the liver of rat, liver adriamycin concentration at the implanted region was obviously higher than that achieved by injection of adriamycin solution. The locally implanted complexes obviously inhibited the growth of subcutaneous implanted tumor of rat, and increased the survival rate of rat with implanted liver tumor.

关键词： hydroxyapatite adriamycin implanted tumor anticancer effect

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成人急性淋巴细胞白血病的诊疗进展

中国癌症杂志 2014年第10期24卷 738-744页

作者：李萍梁爱斌同济大学附属同济医院上海市同济医院血液科

急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)是一种常见的恶性血液疾病。虽然成人ALL患者在诱导缓解治疗后完全缓解率可达80%以上,但大多数患者最终出现复发,长期生存率低。本研究对近年来成人ALL诊疗的进展进行综述,为成人... 详细信息

急性淋巴细胞白血病(acute lymphoblastic leukemia,ALL)是一种常见的恶性血液疾病。虽然成人ALL患者在诱导缓解治疗后完全缓解率可达80%以上,但大多数患者最终出现复发,长期生存率低。本研究对近年来成人ALL诊疗的进展进行综述,为成人ALL患者的诊疗提供参考和依据,以进一步改善该类患者的生存质量。

关键词：成人急性淋巴细胞白血病分子生物学特性费城染色体危险分层造血干细胞移植

Association of Vitamin D Receptor Gene Polymorphisms with Calcium Oxalate Calculus Disease

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第1期23卷 38-41页

作者：王少刚刘继红胡少群叶章群 DepartmentofUrology TongjiHospitalTongjiMedicalCollegeHuazhongUniversityofScienceandTechnology

To study the relationship between polymorphism of vitamin D receptor (VDR) allele with formation of calcium oxalate calculus and find the predisposing genes of calcium oxalate calculus, we screened out 150 patients who suffered from calcium oxalate calculus. 36 of them had idiopathic hypercalciuria according to analysis of calculus component and assay of urine calcium. The polymorphisms of VDR gene Taq1, Apa1 and Fok1 were detected using PCR-RFLP technique and the correlation were analyzed between the polymorphism and urinary calculus or between the polymorphism and hypercalciuria. The difference in each genotypic frequency of the allele of promoter Fok1 between calculus group and healthy group or between idiopathic hypercalciuria calculus group and health group was significant. The content of 24-h urine calcium of those who had genotype ff was obviously higher than that of those who have other genotypes in the same group. There was no significant difference in the polymorphism of gene Apa1 and Taq1 between each two groups. It is concluded that hypercalciuria and calcium oxalate calculus were related to the polymorphism of VDR gene's promoter Fok1 allele, but it had nothing to do with the polymorphism of gene Apa1 and Taq1. The genotype ff was a candidate heredity marker of calcium calculus disease.

关键词： gene polymorphism vitamin D receptor calcium oxalate calculus hypercalciuria

Ziprasidone, haloperidol and clonazepam intramuscular administration in the treatment of agitation symptoms in Chinese patients with schizophrenia: A network meta-analysis

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General Psychiatry 2018年第5期31卷 54-62页

作者： Liang Su Zheng Lu Shenxun Shi Yifeng Xu Shanghai Mental Health Center Shanghai Jiao Tong University School of MedicineShanghaiChina Department of Psychiatry Huashan HospitalFudan UniversitySchool of MedicineShanghaiChina Department of Psychiatry Tongji HospitalTongji UniversitySchool of MedicineShanghaiChina

Background Agitation is very common in patients with acute stage schizophrenia, and injection of antipsychotics and clonazepam is widely used. Network meta-analysis of these comparisons among three injection treatments has been seldom *** To compare the efficacy and safety of various injections for agitation symptoms in Chinese patients with *** Searches were made in PubMed, Embase and Web of Knowledge, Cochrane Library, Wanfang data, CNKI, SinoMed and VIP databases up to 18 February 2018. Standard search strategies were performed by two reviewers according to the Cochrane Review Group. The Consolidated Standards of Reporting Trials statement was used to assess the methodological quality of the studies. STATA was used to perform meta-analysis. The Cochrane Grades of Recommendation, Assessment, Development and Evaluation(GRADE) was used to assess the strength of *** A total of 15 studies were included in the network meta-analysis. There were 11 studies comparing ziprasidone with haloperidol, and four studies comparing haloperidol with clonazepam. The results showed that ziprasidone is more effective than haloperidol and clonazepam(sucra: 77.2, 72.8 and 0) in the treatment of agitation symptoms. There was the effect size(standardised mean difference(SMD)) in the three groups: haloperidol: SMD=2.278, 95% CI 1.836 to 2.719; ziprasidone: SMD=2.536, 95% CI 2.082 to 2.990; and clonazepam: SMD=1.360, 95% CI 0.127 to 2.593. The acceptability was assessed by the incidence of excessive sedation, which showed that ziprasidone and haloperidol were similar with both being superior to clonazepam(sucra: 0.3, 0.7 and 99.0). Ziprasidone had significantly less adverse effects than haloperidol in effects of extrapyramidal system(EPS)(z=5.01, p<0.001). There were no statistically significant differences between haloperidol and ziprasidone in tachycardia and abnormal ECG(z=1.69, p=0.091; z=0.87, p=0.386; respectively). Based on GRADE, the strength

关键词： Network meta-analysis Embase and Web of Knowledge

Establishment of a Simple and Useful Way for Preimplantation Genetic Diagnosis of Chromosomal Diseases

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第3期27卷 315-317页

作者：罗海宁朱桂金刘群陈雯李舟 Department of Gynecology and Obstetrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

In order to establish a simple and useful way for preimplantation genetic diagnosis (PGD) of chromosomal diseases in general IVF laboratory, the methods that are most commonly used in the embryo biopsy, fixation of blastomere and fluorescence in situ hybridization were compared. The three aspects of PGD were analyzed respectively. There was no significant difference in further de- velopment capacity of embryos between mechanical (79.7%) and chemical biopsy group (78.6%) (P>0.05). In this study, more cells were successfully fixed with the Tween/HCL method (93.8%) than with the methanol/acetic acid method (80.5%, P0.05). The hy- bridization efficiency of fluorescence in situ hybridization was 89.5% in successive denaturation method and 90.9% in codenaturation method with the difference being not significant (P>0.05). In conclusion, the mechanical or chemical method, Tween/HCL fixation method and codenaturation fluorescence in situ hybridization method can constitute a simple and useful way for PGD of chro- mosomal diseases.

关键词： biopsy fixation fluorescence in situ hybridization preimplantation genetic diagnosis

Cardioprotective Effects of Melatonin on Recovery of Rat Donor Hearts after 12-Hour Preservation

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2003年第4期23卷 407-410页

作者：高思海李平潘铁成杨辰垣 Department of Thoracic and Cardiac Surgery

The cardioprotective effects of melatonin on recovery of rat donor hearts after 12 h of preservation were investigated. Wistar rats weighing 200 to 250 g ( n =24) were randomly divided into 3 groups. In the non storage group ( n =8), donor hearts were not stored. In the melatonin group ( n =8), donor hearts were stored in 4 ℃ St. Thomas solution with melatonin (0.1 mmo1/L). In the control group ( n =8), donor hearts were stored in 4 ℃ St. Thomas solution only. The coronary flow (CF), cardiac function, coronary vasodilatory response, creatine kinase (CK) and high energy phosphate levels were measured after the hearts had been preserved for 12 h. Transmission electron microscopy was used to examine the microstructural changes after 12 h of preservation. The recovery of cardiac function and coronary vasodilatory response were significantly improved in the melatonin group ( P <0.01). CK release decreased greatly in the melatonin group ( P <0.01). High energy phosphate levels were significantly better preserved in the melatonin group ( P <0.01). Histological findings were much better in the melatonin group than in the control group. These results suggest that melatonin has cardioprotective effects on the recovery of rat donor hearts after 12 h of preservation.

关键词： melatonin donor heart preservation transplantation ischemia reperfusion

Expression of hTERT, p53 and PCNA in Cystitis Glandularis

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2007年第4期27卷 437-439页

作者：位志峰叶章群陈志强 Department of Urology Nanjing General Hospital of Nanjing Military Command of PLA Department of Urology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology

To examine the expression of human telomere reverse transcriptase （hTERT）, p53 and proliferating cell nuclear antigen （PCNA） in cystitis glandulafis, 38 patients were divided into two grouips： group A （including 18 cases of papillary cystitis glandularis） and group B （including 20 subjects with normal bladder mucosa）. All the cases were immunohistochemically examined by using antibodies specifically against p53 and PCNA, and hTERT was determined by in situ hybridization. hTERT was found in 6 cases （33.3%） and p53 was detected in 4 cases （22.2%） in group A, while they were not detected in group B. There were significant differences in hTERT and p53 expression between groups A and B （P〈0.05 for both）. PCNA was detected in 7 cases （38.9%） in group A and 1 case （5.0%） in group B, and significant difference in PCNA expression was found between the two groups （P〈0.05）. The expressions of hTERT, p53 and PCNA were significantly higher in group A than in group B, suggesting that papillary cystitis glandularis is predisposed to cancerous change, and p53, PCNA, hTERT may be related to the malignant alteration.

关键词： cystitis glandularis human telomere reverse transcriptase p53 proliferating cell nuclear antigen

Monitoring of Biological Responses of Tumor Cells after Irradiation with ^(99m)Tc-MIBI——An In Vitro Study

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第2期21卷 152-155页

作者：夏劲松吴华赵明鲜于志群 Department of Nuclear Medicine Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

To explore the possibility to employ 99m Tc MIBI to monitor biological response of tumor cells after irradiation and to observe the relation between the radiation doses and the uptake levels of 99m Tc MIBI in tumor cells, the cells were irradiated with a single dose of 2 Gy, 10 Gy and 20 Gy respectively. The uptake of 99m Tc MIBI in each dosage group was determined before and 24, 48, 72 h after irradiation respectively. Apoptosis index , plating efficiency of tumor cells was simultaneously determined. There was a positive correlation between uptake levels of 99m Tc MIBI and AI. A negative correlation was noted between the uptake levels and PE . It is suggested that 99m Tc MIBI may be used as a tracer to monitor the change of viability state of tumor cells after being irradiated with different doses.

关键词： 99m Tc MIBI tumor cells irradiation biological effect

Study on the Changes in Microvessel Density in Hepatocellular Carcinoma Following Transcatheter Arterial Chemoembolization

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Journal of Huazhong University of Science and Technology(Medical Sciences) 2001年第4期21卷 321-322,331页

作者：易继林廖晓峰杨志芳李兴睿 Department of General Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030

To study the changes in intratumoral microvessel density (MVD) in hepatocellular carcinoma (HCC) following transcatheter arterial chemoembolization (TACE), MVD in 42 HCC specimens histologically verified was studied by using immunohistochemical method. Of all the specimens, 20 were obtained from the patients treated with surgical resection alone, 22 from those with second stage surgical resection after TACE. The results showed that the MVD in HCC tissues was 53.4±21 9 in the TACE group and 27.6±9.2 in the single operating group, respectively, with the difference being significant between them ( P <0.001). It was suggested that TACE might contribute to angiogenesis of HCC, possibly due to anoxic stress and ischemia reperfusion injury.

关键词： hepatocellular carcinoma chemoembolization, therapeutic microvessel density

Triplex operation for portal hypertension with esophageal variceal bleeding:report of 140 cases

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 534-537页

作者： Liu-Shun Feng, Ke Li, Qi-Ping Peng, Xiu-Xian Ma, Yong-Fu Zhao, Pei-Qin Xu and Xiao-Ping Chen Zhengzhou, China Department of General Surgery, First Affiliated Hospi- tal , Zhengzhou University, Zhengzhou 450052 , China Hepatic Surgery Center, tongji hospital, tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China Department of General Surgery First Affiliated Hospital Zhengzhou University Zhengzhou 450052 China.

BACKGROUND:Portal hypertension is a common dis ease. The surgical therapy of this disease focuses on the re sultant upper digestive tract bleeding, which can imperi patients' life directly. This study was to evaluate the effect of triplex operation ( mesocaval C shunt with artificia graft, ligation of the coronary vein and splenic artery) on portal hypertension and its associated upper digestive tract bleeding. METHODS: A retrospective study was made on clinical da- ta of 140 patients undergoing triplex operation, who had suffered from portal hypertension and upper digestive tract bleeding. RESULTS: Postoperative portal pressure was 25-43 cmH2 O ( preoperative portal pressure 27-45 cmH2 O ) with the average reduction of 10 cmH2O. One patient (0.7%) died of cerebrovascular disease. Five patients (3.5%) suffered from mild hepatic encephalopathy, which was ameliorated through conservative treatment. Lymphatic fistula occurred in 3 patients (2.1% ) who recovered without treatment 5, 10 days and 3 months after operation respectively. One hundred patients were followed up for 1 month to 6 years without recurrent hemorrhage or hepatic encephalopathy. Hypersplenism and ascites disappeared in 70 patients (70% ) and 80 patients (80% ) respectively. A significant reduction of ascites was seen in 12 patients(12% ). The arti- ficial vessels remained unblocking detected by B type ultra- sonography and Doppler sonography in 95 patients (95% ). CONCLUSION: Triplex operation is suitable for patients with the following portal hypertensions; portal hyperten- sion caused by simple occlusion of the hepatic vein (a patho- logical type of Budd-Chiari syndrome); thrombosis of the portal vein or prehepatic portal hypertension because of cavernous transformation; intrahepatic portal hypertension with rebleeding after splenectomy or non-operation, and those patients with liver function in grade A or B according to the Child-Pugh classification.

关键词： hypertension portal vein esophageal and gastric varices hemorrhage gastrointestinal