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Identification and Analysis of miRNAs and siRNAs in Botrytis cinerea

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American Journal of Plant Sciences 2022年第5期13卷 623-649页

作者： Yaoyao Liu Miao Sun Qinru Yu Ran Li Xu Hu Pengcheng Zhang tongfei lai Ting Zhou College of Life and Environmental Science Hangzhou Normal University Hangzhou China

Small RNAs in Botrytis cinerea were analyzed via high-throughput sequencing on BGISEQ-500 platform. A total of 8 novel miRNAs and 110 novel siRNAs were predicted. Sequence information, construction, length distribution, base bias and expression levels of miRNAs and siRNAs were determined as well. Through GO and KEGG enrichment analysis, the miRNA target genes are mostly located in membrane and organelle, possessed binding and catalytic activities, and involved in signal transduction and carbohydrate metabolism. The results will provide a theoretical foundation for understanding the developmental and pathogenic mechanisms of B. cinerea at the transcriptional level.

关键词： Botrytis cinerea MicroRNA Small Interfering RNA Biological Function

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界面吸附对高分子液体膜去润湿动力学的影响

高等学校化学学报 2024年第5期45卷 149-154页

作者：张苡宁卫来石彤非徐林伊犁师范大学物理科学与技术学院新疆凝聚态相变与微结构重点实验室伊宁835000 广东工业大学轻工化工学院广州510006 中国科学院深圳先进技术研究院珠海中科先进技术研究院有限公司生物材料中心珠海519000

以线形聚苯乙烯(LPS)薄膜三臂星形聚苯乙烯(3SPS)薄膜作为研究对象,探讨了受限体系中高分子链界面吸附对高分子溶液膜界面稳定性的影响.实验结果表明,吸附在基底上的高分子链对薄膜去润湿的驱动力形成拖拽力,增大了薄膜去润湿的阻力.随... 详细信息

以线形聚苯乙烯(LPS)薄膜三臂星形聚苯乙烯(3SPS)薄膜作为研究对象,探讨了受限体系中高分子链界面吸附对高分子溶液膜界面稳定性的影响.实验结果表明,吸附在基底上的高分子链对薄膜去润湿的驱动力形成拖拽力,增大了薄膜去润湿的阻力.随着退火时间的延长,线形链和三臂星形链在基底上的吸附量逐渐增加,LPS和3SPS薄膜在丙酮蒸汽中由发生去润湿到稳定存在,孔边在薄膜去润湿过程中由不稳定转变为稳定,去润湿速度逐渐降低,平衡接触角逐渐减小.当退火时间相同时,3SPS薄膜在丙酮蒸汽中的去润湿速度比LPS薄膜更低;去润湿后3SPS薄膜在基底上的平衡接触角比LPS薄膜更小,3SPS薄膜的毛细驱动力小于LPS薄膜.

关键词：高分子薄膜去润湿界面吸附

In Search of Regulators of LeSPL-CNRby South-Western Blotting and Yeast One-Hybrid Library Screening System

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American Journal of Plant Sciences 2018年第5期9卷 1037-1050页

作者： Xiaohong Wang Bishun Ye Ying Wang Ting Zhou tongfei lai Research Centre for Plant RNA Signaling College of Life and Environmental Sciences Hangzhou Normal University Hangzhou China Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City College of Life and Environmental Science Hangzhou Normal University Hangzhou China

LeSPL-CNR is a crucial transcription factor for fruit ripening of Solanum lycopersicum. The cnr (colorless non-ripening) epimutation resulted from hypermethylation in a 286 bp region of LeSPL-CNR promoter inhibits normal fruit ripening. In present study, potential regulators of LeSPL-CNR, which could bind to the specific 286 bp region, were screened via south-western blotting and yeast one-hybrid (Y1H) library screening system. Results indicated that a total of 13 and 19 candidate proteins were acquired respectively, and both ribulose-1,5-bisphosphate carboxylase/oxygenase and 40S ribosomal protein were identified by two methods. These would provide some information for revealing roles of DNA methylation and the regulatory mechanism for LeSPL-CNR.

关键词： Solanum lycopersicum LeSPL-CNR 286 bp Region South-Western Blotting Yeast One-Hybrid

A Novel Protection and Utilization Pattern for Urban Wetland—As an Example of Hemu Wetland in Hangzhou, China

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Journal of Water Resource and Protection 2015年第4期7卷 300-311页

作者： Nongnong Shi Minger Ye tongfei lai Zhiming Yu Yunli Pan Mengying Yu Xiaoqi Zhou School of Life and Environmental Sciences Hangzhou Normal University Hangzhou China School of Agriculture and Biological Technology Zhejiang University Hangzhou China

This paper proposes an innovative plan and its implement strategy of Hemu urban wetland. The plan takes into consideration its historical background and current status, its protection significance, strategies, solutions and support conditions. It highlights the landscape characteristics of rivers and lakes, and restores its historical flood prevention functions and climate regulation for the city. Especially, this paper proposes to achieve the restoration of “Mulberry-sided Fish Pond” farming style—an important agricultural heritage created by the ancestors inside the wetland, which is a unique and creative way to use low land for ecological agriculture and cycling economy. The proposed wetland sewage treatment schemes would be of practical importance to improve and enhance the ecological environment and promote the construction of ecological civilization in the region. The “Two zones—One block and Three complexes” protection and utilization plan is reasonable and feasible. It fully presents the functions of the wetland for its production, living, life forms and ecology, and is characterized by joint protection of the wetland by original residents and social public. This will result in active, instead of passive, wetland protection, like turning a “blood transfusion protection” to “hematopoietic protection”, which reflects the sustainable development. The paper offers valuable information and strategies for planning and development of urban wetland.

关键词： Urban Wetland Protection and Utilization Mulberry-Sided Fish Pond Ecosystem Two Zones—One Block and Three Complexes

Proteomics-Based Analysis of Phalaenopsis amabilisin Response toward CymbidiumMosaic Virus and/or OdontoglossumRingspot Virus Infection

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American Journal of Plant Sciences 2013年第9期4卷 1853-1862页

作者： tongfei lai Yanfu Deng Pengchen Zhang Zhijuan Chen Feng Hu Qi Zhang Yihua Hu Nongnong Shi Research Centre of Plant Signaling School of Life and Environmental Sciences Hangzhou Normal University Hangzhou China.

Stress response at the protein level to viral infection in orchid plants has not been extensively investigated to date. To understand the proteomic basis of Phalaenopsis amabilis’s responses to Cymbidium Mosaic virus (CymMV), and/or Odontoglossum ring spot virus (ORSV), the total proteins were extracted from Phalaenopsis amabilis leaves infected with CymMV, ORSV, or both respectively. Differentially expressed proteins were identified by two-dimensional electrophoresis, and 27 of these proteins that had significant changes were further examined by mass spectrometry. Comparing CymMV-infected leaves with mock-inoculated ones, 2 proteins were significantly up-regulated, 9 were significantly down-regulated and 1 previously undetected protein was identified. 10 proteins were significantly up-regulated, 3 significantly down-regulated and 1 previously undetected protein was identified in ORSV-infected leaves. 6 proteins were significantly up-regulated and 9 significantly down-regulated proteins were found in co-infected leaves. These identified proteins are involved in disease resistance, stress response, transcriptional regulation, energy metabolism, protein modification and the previously unknown proteins were not involved with known protein pathways. Proteins significantly up-regulated were ATP sulfurylase, down-regulated proteins included glutamate decarboxylase isozyme 2, RNA polymerase alpha subunit and chloroplastic peptide deformylase 1A were proteins with similar alteration trend after all infection treatments. Significantly up-regulated were Thioredoxin H-type and down-regulated Cytosolic phosphoglycerate kinase I which were proteins that have been shown to be specifically regulated by the infection with CymMV. Significantly up-regulated were proteins like Rubisco large subunit, Triosephosphate isomerase, NADP-specific isocitrate dehydrogenase and Cinnamoyl CoA reductase CCR2 by the infection of ORSV. Protein regulation in coinfected leaves followed a pattern simi

关键词： Phalaenopsis amabilis Two-Dimensional Electrophoresis Mass Spectrometry Cymbidium Mosaic Virus Odontoglossum Ringspot Virus

锐顶镰孢菌Fusarium acuminatum小RNA的鉴定与分析

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杭州师范大学学报（自然科学版） 2022年第4期21卷 403-411,432页

作者：李冉夏旋燕孙苗周婷赖童飞杭州师范大学生命与环境科学学院浙江杭州311121

用高通量测序技术分析了Fusarium acuminatum细胞内小RNA的组成、长度分布、碱基偏好性、分类以及潜在的调控靶标,预测了11个新的miRNAs和6503个潜在的siRNAs.其中,miRNAs的靶基因多定位于膜组织,具有催化或结合活性,主要与代谢相关.研... 详细信息

用高通量测序技术分析了Fusarium acuminatum细胞内小RNA的组成、长度分布、碱基偏好性、分类以及潜在的调控靶标,预测了11个新的miRNAs和6503个潜在的siRNAs.其中,miRNAs的靶基因多定位于膜组织,具有催化或结合活性,主要与代谢相关.研究结果为农业生产中合理有效防治***引起的病害提供了理论基础.

关键词： Fusarium acuminatum 小RNA miRNA siRNA

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基于蛋白质组学的癸醛抑制扩展青霉机制分析

杭州师范大学学报（自然科学版） 2023年第4期22卷 411-419页

作者：俞沁如刘瑶瑶李冉胡奕然王寒怡赖童飞杭州师范大学生命与环境科学学院浙江杭州311121

扩展青霉是果蔬采后病害主要致病菌之一,对果蔬产业具有巨大的危害.植物精油主要抑菌成分癸醛能够显著抑制扩展青霉的生长发育,并对扩展青霉诱导的苹果青霉病具有较好的防治效果.利用iTRAQ技术探讨了外源癸醛对扩展青霉蛋白质组水平的影... 详细信息

扩展青霉是果蔬采后病害主要致病菌之一,对果蔬产业具有巨大的危害.植物精油主要抑菌成分癸醛能够显著抑制扩展青霉的生长发育,并对扩展青霉诱导的苹果青霉病具有较好的防治效果.利用iTRAQ技术探讨了外源癸醛对扩展青霉蛋白质组水平的影响,结果表明,基于差异表达蛋白分析,癸醛处理能够破坏扩展青霉胞内氧化还原状态的平衡,抑制胞内氨基酸代谢,影响隔膜蛋白的表达和空间结构,进而抑制扩展青霉的生长发育.研究结果为阐明癸醛的抑菌机制提供了理论基础.

关键词：扩展青霉癸醛蛋白质组抑菌机制

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江山白菇F21品系菇根的生化特性分析

食品科学 2016年第17期37卷 90-95页

作者：程小华汪莹戚琴芹王娅妮赖童飞周婷浙江商业职业技术学院旅游烹饪学院浙江杭州310053 杭州师范大学生命与环境科学学院农产品质量安全控制技术研究杭州市重点实验室浙江杭州310036

以金属含量、氨基酸组成、差异表达蛋白以及抗氧化酶活性为指标对江山白菇F_(21)品系菇根的生化组成进行了分析。结果表明:与菇身相比,菇根中粗脂肪、铁、磷以及必需氨基酸中的亮氨酸和赖氨酸含量较低,而灰分、砷、钙和镁元素含量较高;... 详细信息

以金属含量、氨基酸组成、差异表达蛋白以及抗氧化酶活性为指标对江山白菇F_(21)品系菇根的生化组成进行了分析。结果表明:与菇身相比,菇根中粗脂肪、铁、磷以及必需氨基酸中的亮氨酸和赖氨酸含量较低,而灰分、砷、钙和镁元素含量较高;通过菇根与菇身的比较蛋白质组学分析,共鉴定出9种差异表达蛋白,涉及双组分信号转导系统、乙酰辅酶A代谢、蛋白质合成、乙醛酸及二羧酸代谢、氮代谢多个生物学过程,同时菇根中的菇毒素含量显著低于菇身;货架期菇根中的抗氧化酶活性较低。

关键词：江山白菇F21品系菇根氨基酸组成比较蛋白质组学抗氧化酶活性

番茄果实成熟过程中色素合成相关基因的表达分析

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杭州师范大学学报（自然科学版） 2020年第4期19卷 382-390页

作者：陈远志孙杨莹叶必顺赖童飞杭州师范大学生命与环境科学学院植物RNA信号传导中心浙江杭州311121

对番茄果实成熟过程中色素含量进行检测,并利用实时定量PCR技术对色素合成相关基因的表达进行分析.结果表明,番茄果实成熟过程中,叶绿素含量逐渐降低,花青素含量变化不显著,番茄红素及类黄酮含量显著升高,大部分调控色素合成的转录因子... 详细信息

对番茄果实成熟过程中色素含量进行检测,并利用实时定量PCR技术对色素合成相关基因的表达进行分析.结果表明,番茄果实成熟过程中,叶绿素含量逐渐降低,花青素含量变化不显著,番茄红素及类黄酮含量显著升高,大部分调控色素合成的转录因子基因、类胡萝卜素代谢途径以及类黄酮代谢途径相关基因在果实破色后呈现先上升后下降的趋势.

关键词：番茄成熟色素合成转录因子类胡萝卜素类黄酮