Uncovering Small RNAs in Penicillium digitatum by Transcriptome Sequencing
Uncovering Small RNAs in Penicillium digitatum by Transcriptome Sequencing作者机构：College of Life and Environmental Science Hangzhou Normal University Hangzhou China Worcester-Hangzhou Joint Molecular Plant Health Laboratory School of Science and the Environment University of Worcester Worcester UK
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2022年第13卷第7期
页 面：1006-1022页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Penicillium digitatum Transcriptome Sequencing MicroRNA Small Interfering RNA
摘 要：Small RNAs in Penicillium digitatum were identified and analyzed via transcriptome sequencing on the BGISEQ-500 platform. A total of 15 predicted miRNAs and 10718 novel siRNAs were found. Their length distribution, sequence, predicted construction, base bias, expression levels and potential targets were determined as well. Through pathway and KEGG enrichment analysis, the miRNA target genes were mostly involved in carbohydrate metabolism, transport and catabolism, translation and amino acid metabolism. The target genes involved in aflatoxin biosynthesis and proteasome had a higher rich factor value. The results will provide a theoretical foundation for understanding the developmental and pathogenic mechanisms of P. digitatum at the transcriptional level.
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