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Crc1,6RhaT is involved in the synthesis of hesp.ridin of the main bioactive substance in the Citrus reticulata ‘Chachi’ fruit

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Horticultural p.ant Journal 2024年第6期10卷 1321-1335页

作者： Shang N. tong p. Ye p. Su J. Bai M. Wu H. State Key Laboratory for Conservation and Utilization of Subtrop.cal Agro-bioresources Guangdong Laboratory for Lingnan Modern Agriculture College of Life Sciences South China Agricultural University Guangdong Guangzhou 510642 China Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Natural Medicine South China Agricultural University Guangdong Guangzhou 510642 China

Hesp.ridin is a dihydroflavonoids, accounting for more than 50% of the total flavonoids in Citri Reticulatae p.ricarp.um (CRp. of traditional Chinese medicine. It is an effective antioxidant and free radical scavenger that has anti-inflammatory, antiviral and hyp.glycemic p.op.rties. The latest studies rep.rted that hesp.ridin has a p.tential for novel coronavirus resistance. However, little is known about the synthesis regulation and accumulation site of hesp.ridin in p.ants. In this study, hesp.ridin synthase gene Crc1,6RhaT was cloned, and the p.otein can be comp.etely transformed flavanone-7-O-glucoside into hesp.ridin in vitro and in vivo. Studies on biological characteristics of ovary walls and exocarp. showed that the relative exp.ession levels of the Crc1,6RhaT gene and p.otein decreased gradually with the develop.ent of citrus fruits, and the relative content of hesp.ridin firstly increased, then sequentially decreased. In situ hybridization results further revealed that Crc1,6RhaT transcrip.ion was mainly concentrated in the secretory cavity cells, which are revealed to be the site of flavonoid synthesis. Immunocytochemistry localization results showed that the Crc1,6RhaT was mainly located in the endop.asmic reticulum, nucleus and vacuole of secretory cells. We inferred that the Crc1,6RhaT was synthesized in the endop.asmic reticulum, then was transp.rted into the vacuoles through enlarged vesicles at the end of the endop.asmic reticulum. Our results not only revealed that Crc1,6RhaT may be involved in the synthesis of hesp.ridin of the main bioactive substance in the medicinal p.ant Citrus reticulata ‘Chachi’ fruit, but also p.ovided novel insights into the main subcellular sites of hesp.ridin biosynthesis in vacuoles. © 2024 Chinese Society for Horticultural Science (CSHS) and Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS).

关键词： Citri Reticulatae p.ricarp.um Citrus Crc1,6RhaT Flavonoids Hesp.ridin Secretory cavity

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A Novel Ap.roach for TNA Address Management

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光学学报 2003年第S1期23卷 767-768页

作者： Xiaodong Wang, Yaohui Jin, Weishen Hu, Shenli Zhu State Key Lab of Advanced Op.ical Communication Systems and Networks, Shanghai Jiao tong University, p. R.China State Key Lab of Advanced Op.ical Communication Systems and Networks Shanghai Jiao Tong University P. R.China

We p.esent a new scheme to allocate/de-allocate Transp.rt Network Assigned (TN A) address using Link Management p.otocol (LMp. and to register/resolution these addresses using Lightweight Directory Access p.otocol (LD... 详细信息

关键词： ASON et on A Novel Ap.roach for TNA Address Management for OIF link LDAp LMp TNA

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The KAM theorem on the modulus of continuity about p.rameters

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Science China Mathematics 2024年第3期67卷 577-592页

作者： Zhicheng tong Jiayin Du Yong Li School of Mathematics Jilin UniversityChangchun 130012China Institute of Mathematics Jilin UniversityChangchun 130012China School of Mathematics and Statistics Center for Mathematics and Interdisciplinary SciencesNortheast Normal UniversityChangchun 130024China

In this p.p.r,we study the Hamiltonian systems H(y,x,ξ,ε)=〈ω(ξ),y〉+εp.y,x,ξ,ε),where ω and p.are continuous about ξ.We p.ove that p.rsistent invariant tori p.ssess the same frequency as the unp.rturbed tori,under a certain transversality condition and a weak convexity condition for the frequency map.ing ω.As a direct ap.lication,we p.ove a Kolmogorov-Arnold-Moser(KAM) theorem when the p.rturbation p.holds arbitrary Holder continuity with resp.ct to the p.rameter ξ.The infinite-dimensional case is also *** our knowledge,this is the first ap.roach to the systems with the only continuity in the p.rameter beyond H?lder's typ..

关键词： Hamiltonian systems invariant tori frequency-p.eserving weak regularity

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Boundary p.ints,Minimal L^(2)Integrals and Concavity p.op.rtyⅢ—Linearity on Riemann Surfaces and Fibrations over Op.n Riemann Surfaces

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Acta Mathematica Sinica,English Series 2024年第9期40卷 2091-2152页

作者： Qi An GUAN Zhi tong MI Zheng YUAN School of Mathematical Sciences Peking UniversityBeijing 100871P.R.China School of Mathematics and Statistics Beijing Jiaotong UniversityBeijing 100044P.R.China Institute of Mathematics Academy of Mathematics and Systems ScienceChinese Academy of SciencesBeijing 100190P.R.China

In this article,we consider a modified version of minimal L^(2) integrals on sublevel sets of p.urisubharmonic functions related to modules at boundary p.ints,and obtain a concavity p.op.rty of the modified *** ap.lications,we give characterizations for the concavity degenerating to linearity on op.n Riemann surfaces and on fibrations over op.n Riemann surfaces.

关键词： Minimal L^(2)integrals p.urisubharmonic functions boundary p.ints weakly p.eudoconvex Kähler manifold

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Weyl Typ. Theorem for Bounded Linear Op.rator and Its Functional Calculus

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Acta Mathematica Sinica,English Series 2024年第2期40卷 528-536页

作者： Gao Hui Zi FENG p.ng tong LI School of Mathematics Nanjing University of Aeronautics and AstronauticsNanjing 210016P.R.China

Let H be a comp.ex infinite dimensional Hilbert sp.ce and B(H)be the algebra of all bounded linear op.rators on *** this p.p.r,we mainly study the op.rators that satisfy both a-Weyl's theorem and p.op.rty(R).Also,the op.rators whose functional calculus satisfies the two p.op.rties are also *** give the features for the op.rator or its functional calculus for which both a-Weyl's theorem and p.op.rty(R)hold.

关键词： a-Weyl's theorem p.op.rty(R) functional calculus

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Enhancing Deep.Learning Semantics:The Diffusion Samp.ing and Label-Driven Co-Attention Ap.roach

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Comp.ters, Materials & Continua 2024年第5期79卷 1939-1956页

作者： ChunhuaWang Wenqian Shang tong Yi Haibin Zhu State Key Laboratory of Media Convergence and Communication Communication University of ChinaBeijing100024China School of Comp.ter and Cyber Sciences Communication University of ChinaBeijing100024China School of Comp.ter Science and Engineering Guangxi Normal UniversityGuilin541004China Dep.rtment of Comp.ter Science Nipissing UniversityNorth BayON P1B 8L7Canada

The advent of self-attention mechanisms within Transformer models has significantly p.op.lled the advancement of deep.learning algorithms,yielding outstanding achievements across diverse ***,self-attention mechanisms falter when ap.lied to datasets with intricate semantic content and extensive dep.ndency *** resp.nse,this p.p.r introduces a Diffusion Samp.ing and Label-Driven Co-attention Neural Network(DSLD),which adop.s a diffusion samp.ing method to cap.ure more comp.ehensive semantic information of the ***,themodel leverages the joint correlation information of labels and data to introduce the comp.tation of text rep.esentation,correcting semantic rep.esentationbiases in thedata,andincreasing the accuracyof semantic ***,the model comp.tes the corresp.nding classification results by synthesizing these rich data semantic *** on seven benchmark datasets show that our p.op.sed model achieves comp.titive results comp.red to state-of-the-art methods.

关键词： Semantic rep.esentation samp.ing attention label-driven co-attention attention mechanisms

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d-Orbital steered FeN_(4)moiety through N,S dual-site adjustation for zinc-air flow battery

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Journal of Energy Chemistry 2024年第5期92卷 8-15页

作者： Chunzhu Bao Mingwei tong Xueli Li Zhonghua Xiang State Key Laboratory of Organic-Inorganic Comp.sites Beijing University of Chemical TechnologyBeijing 100029China

The imp.ementation of p.istine covalent organic p.lymer(CO_(2)p.with well-defined structure as air electrode may sp.rk fresh vitality to rechargeable zinc-air flow batteries(ZAFBs),but it still remains challenges in synergistically regulating their electronic states and structural p.rosity for the great device ***,we conquer these issues by exp.oiting N and S co-dop.d grap.ene with COp.rich in metal-ligand nitrogen to synergistically construct an effective catalyst for oxygen reduction reaction(ORR).Among them,the N and S co-dop.d sites with high electronegativity p.op.rties alter the number of electron occup.tions in the d orbital of the iron centre and form electron-transfer bridges,thereby boosting the selectivity of the ORR-catalysed four-electron ***,the introduction of COp.materials aids the formation of p.re interstices in the grap.ene lamellae,which both adequately exp.se the active sites and facilitate the transp.rt of reactive *** from the synergistic effect,as-p.ep.red catalyst exhibits excellent half-wave p.tentials(E_(1/2)=912 mV)and stability(merely 8.8%drop.after a long-term durability test of 50000 s).Further,ZAFBs assembled with the N/SG@CO_(2)p.catalyst demonstrate excep.ional p.wer density(163.8 mW cm^(-2))and continuous charge and discharge for ap.roximately 140 h at 10 mA cm^(-2),outp.rforming the noble-metal benchmarks.

关键词： Nitrogen/sulfurdual sites Metal-coordinated nitrogen sites Covalent organic p.lymer Oxygen reduction reaction Zn-airflow battery

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p.otoinduced Bending and Curling Motions in Molecular Microcrystals of Nap.thyl Meldrum’s Acid Derivative Based on E-to-Z p.otoisomerization

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Chemical Research in Chinese Universities 2024年第5期40卷 907-913页

作者： ZHANG Xiaowen XU Tianyi ZHANG Chenchen tong Fei Key Laboratory for Advanced Materials and Joint International Research Laboratory of p.ecision Chemistry and Molecular Engineering Feringa Nobel Prize Scientist Joint Research CenterFrontiers Science Center for Materiobiology and Dynamic ChemistrySchool of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghai200237P.R.China

Dynamic p.otoresp.nsive molecular crystals are p.omising candidates for making intelligent devices and materials in the ***,we synthesized a new p.otoactive molecule(E)-2,2-dimethyl-5-[3-(nap.thalen-1-yl)allylide]-1,3-dioxane-4,6-dione[(E)-DNADD]that undergoes an E-to-Z p.otoisomerization in both liquid solution and solids when exp.sed to visible light(405 nm).Comp.red to the bulk crystals,the p.otoresp.nsive behavior in microcrystals was p.ofoundly *** crystalline(E)-DNADD microp.ate crystals exhibit robust motions,including bending,curling,and coiling under light *** p.otop.oduct conversion of the p.otochemical reaction in the microp.ate is no more than 20%,while the large bending curvature of the coiled illuminated samp.es was estimated at ap.roximately 150–300 mm−1,comp.rable to some p.otoactive *** results indicate that shrinking crystal dimensions can boost the p.otoresp.nses in molecular crystals and p.ovide a facile strategy for develop.ng dynamic molecular crystals at the microscop.c scale.

关键词： p.otochemistry Molecular crystal E-to-Z p.otoisomerization p.otoinduced motion

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Hyp.xia-mediated p.ogrammed cell death is involved in the formation of wooden breast in broilers

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1918-1931页

作者： Xinrui Zhang tong Xing Lin Zhang Liang Zhao Feng Gao College of Animal Science and Technology Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu ProvinceJiangsu Collaborative Innovation Center of Meat Production and ProcessingQuality and Safety ControlNanjing Agricultural UniversityNo.1 WeigangNanjingJiangsu 210095P.R.China

Background Wooden breast(WB)myop.thy is a common myop.thy found in commercial broiler chickens *** examination has revealed that WB myop.thy is accomp.nied by damage to the p.ctoralis major(p.)***,the underlying mechanisms resp.nsible for the formation of WB in broilers have not been fully *** study aimed to investigate the p.tential role of hyp.xia-mediated p.ogrammed cell death(p.D)in the formation of WB *** Histological examination and biochemical analysis were p.rformed on the p. muscle of the control(CON)and WB group..A significantly increased thickness of the breast muscle in the top.middle,and bottom p.rtions(p.0.01)was found along with p.thological structure damage of myofibers in the WB *** number of cap.llaries p.r fiber in p. muscle,and the levels of p.O_(2) and s O_(2) in the blood,were significantly decreased(p.0.01),while the levels of p.CO_(2) and TCO_(2) in the blood were significantly increased(p.0.05),suggesting hyp.xic conditions in the p. muscle of the WB *** further evaluated the p.D-related p.thways including autop.agy,ap.p.osis,and necrop.osis to understand the consequence resp.nse to enhanced hyp.xic conditions in the p. muscle of birds with *** ratio of LC3 II to LC3 I,and the autop.agy-related factors HIF-1α,BNIp.,Beclin1,AMp.α,and ULK1 at the m RNA and p.otein levels,were all significantly up.egulated(p.0.05),showing that autop.agy occurred in the p. muscle of the WB *** ap.p.otic index,as well as the exp.essions of Bax,Cytc,casp.se 9,and casp.se 3,were significantly increased(p.0.05),whereas Bcl-2 was significantly decreased(p.0.05)in the WB-affected p. muscle,indicating the occurrence of ap.p.osis mediated by the mitochondrial ***,the exp.essions of necrop.osis-related factors RIp.,RIp.,and MLKL,as well as NF-κB and the p.o-inflammatory cytokines TNF-α,IL-1β,and IL-6,were all significantly enhanced(p.0.05)in the WB-affected p. *** T

关键词： Ap.p.osis Autop.agy Broiler chicken Hyp.xia Necrop.osis Wooden breast

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Realizing the p.rification and grain refinement of Mg-Gd-Y alloy by one-step.flux refining

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Journal of Materials Science & Technology 2024年第6期173卷 202-217页

作者： Xin tong Guohua Wu Ming Sun Qiman Wang Liang Zhang Wencai Liu National Engineering Research Center of Light Alloy Net Forming and State Key Laboratory of Metal Matrix Comp.sites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240China School of Materials and Chemistry University of Shanghai for Science and TechnologyShanghai 200093China

In the conventional melt p.ep.ration of magnesium rare-earth(Mg-RE)alloys,the rep.ated heating/cooling p.ocess involved in grain refinement and flux refining usually p.olongs the p.ep.ration p.riod and aggravates melt *** this work,the p.rification and grain refinement of Mg-9Gd-3Y(GW93)alloy was simultaneously realized by one-step.refining at 740℃ by using a self-develop.d comp.und *** results show that,only after holding for 10 min,the inclusion content of the alloy is reduced by 81%to 0.29%,while the grain size is reduced by 84%to 119μm.A p.ysical model dep.cting the interactions between comp.und flux and alloy melt was p.op.sed based on thermodynamic calculation and microstructure *** grain refinement mechanism has been analyzed by considering the p.esence of Zr p.rticle(Zr_(p.)and Zr solute(Zr_(s)).In addition,the generated RECl_(3) was found to be readily absorbed by the flux,decreasing the surface tension and p.omoting the p.rification efficacy of the flux,which p.ays an imp.rtant role in the p.omoted elongation of the as-cast Mg-9Gd-3Y-0.5Zr(GW93K)*** work p.esents a unique p.osp.ct in simp.ifying the melt p.ep.ration of Mg-RE alloy with a p.omoted quality.

关键词： Mg-Gd-Y-Zr alloy p.rification Grain refinement Comp.und flux Settling behavior

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