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内皮型一氧化氮合酶与血管结构及功能的相关性

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中国临床康复 2005年第15期9卷 62-63,i001页

作者：韩鹂孙雪梅乔彤南京大学医学院附属鼓楼医院科研部江苏省南京市210008

目的:应用内部核糖体进入位点构建携带内皮型一氧化氮合酶和血管内皮生长因子的双基因表达载体,研究外源性基因内皮型一氧化氮合酶和血管内皮生长因子在人脐静脉内皮细胞ECV304中的转染表达,探讨内皮型一氧化氮合酶与血管结构及功能的... 详细信息

目的:应用内部核糖体进入位点构建携带内皮型一氧化氮合酶和血管内皮生长因子的双基因表达载体,研究外源性基因内皮型一氧化氮合酶和血管内皮生长因子在人脐静脉内皮细胞ECV304中的转染表达,探讨内皮型一氧化氮合酶与血管结构及功能的相关性。方法:实验于2003-12/2004-12在南京鼓楼医院科研部完成。将Phcmvsp1a-enos中内皮型一氧化氮合酶基因和PcDNA-vegf中血管内皮生长因子基因定向克隆到真核表达载体PcDNA-ires中,构建重组质粒PcDNA-enos-ires-vegf,经酶切,聚合酶链反应扩增和部分DNA测序分析证实后,转染人脐静脉内皮细胞,硝酸还原酶法检测转染后细胞中一氧化氮和一氧化氮合酶活性,免疫组化和荧光双标记及westernblotting检测转染后细胞蛋白水平的表达,Trizol提取总RNA,反转录-聚合酶链反应测定转染后细胞mRNA水平的表达。结果:成功构建携带内皮型一氧化氮合酶和血管内皮生长因子的双基因表达载体PcDNA-enos-ires-vegf,双基因内皮型一氧化氮合酶和血管内皮生长因子在人脐静脉内皮细胞基因组中获得整合及表达。重组基因PcDNA-enos-ires-vegf组与对照组PcDNA-enos在一氧化氮含量和一氧化氮合成酶活性的表达差异无显著性意义。PcDNA-enos-ires-vegf转染细胞后转染效率为30%～40%。

关键词：血管结构相关性人脐静脉内皮细胞内皮型一氧化氮合酶基因双基因表达载体血管内皮生长因子基因内部核糖体进入位点一氧化氮合成酶活性聚合酶链反应扩增一氧化氮合酶活性 blotting DNA测序分析 western ECV304 真核表达载体

TabHLH27 orchestrates root growth and drought tolerance to enhance water use efficiency in wheat

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Journal of Integrative Plant Biology 2024年第7期66卷 1295-1312页

作者： Dongzhi Wang Xiuxiu Zhang Yuan Cao Aamana Batool Yongxin Xu Yunzhou qiao Yongpeng Li Hao Wang Xuelei Lin Xiaomin Bie Xiansheng Zhang Ruilian Jing Baodi Dong Yiping tong Wan Teng Xigang Liu Jun Xiao Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Center for Agricultural Resources Research Institute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuang 050022China Key Laboratory of Crop Biology College of Life SciencesShandong Agricultural UniversityTai'an 271018China State Key Laboratory of Crop Gene Resources and Breeding Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Research Center of the Basic Discipline of Cell BiologyHebei Collaboration Innovation Center for Cell Signaling and Environmental AdaptationHebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang 050024China Centre of Excellence for Plant and Microbial Science(CEPAMS) JIC‐CASBeijing 100101China

Cultivating high-yield wheat under limited water resources is crucial for sustainable agriculture in semiarid *** water scarcity,plants activate drought response signaling,yet the delicate balance between drought tolerance and development remains *** genome-wide association studies and transcriptome profiling,we identified a wheat atypical basic helix-loop-helix(b HLH)transcription factor(TF),Tab HLH27-A1,as a promising quantitative trait locus candidate for both relative root dry weight and spikelet number per spike in *** HLH27-A1/B1/D1 knockout reduced wheat drought tolerance,yield,and water use efficiency(WUE).Tab HLH27-A1 exhibited rapid induction with polyethylene glycol(PEG)treatment,gradually declining over *** activated stress response genes such as Ta CBL8-B1 and Ta CPI2-A1 while inhibiting root growth genes like Ta SH15-B1 and Ta WRKY70-B1 under short-term PEG *** distinct transcriptional regulation of Tab HLH27-A1 involved diverse interacting factors such as Ta ABI3-D1 and Tab *** variations of Tab HLH27-A1influence its transcriptional responses to drought stress,with Tab HLH27-A1^(Hap-II)associated with stronger drought tolerance,larger root system,more spikelets,and higher WUE in ***,the excellent Tab HLH27-A1^(Hap-II)was selected during the breeding process in China,and introgression of Tab HLH27-A1^(Hap-II)allele improved drought tolerance and grain yield,especially under water-limited *** study highlights Tab HLH27-A1's role in balancing root growth and drought tolerance,providing a genetic manipulation locus for enhancing WUE in wheat.

关键词： drought tolerance GWAS root growth wheat WUE

Thermal noise-driven resonant sensors

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Microsystems & Nanoengineering 2024年第3期10卷 259-271页

作者： Yan qiao Alaaeldin Elhady Mohamed Arabi Eihab Abdel-Rahman Wenming Zhang School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghaiChina Department of Systems Design Engineering University of WaterlooWaterlooONCanada

MEMS/NEMS resonant sensors hold promise for minute mass and force ***,one major challenge is that conventional externally driven sensors inevitably encounter undesired intrinsic noise,which imposes a fundamental limitation upon their signal-to-noise ratio(SNR)and,consequently,the ***,this restriction becomes increasingly pronounced as sensors shrink to the *** this work,we propose a counterintuitive paradigm shift that turns intrinsic thermal noise from an impediment to a constituent of the sensor by harvesting it as the driving force,obviating the need for external actuation and realizing‘noise-driven’*** sensors employ the dynamically amplified response to thermal noise at resonances for stimulus *** demonstrate that lightly damped and highly compliant nano-structures with high aspect ratios are promising candidates for this class of *** overcome the phase incoherence of the drive force,three noise-enabled quantitative sensing mechanisms are *** validated our sensor paradigm by experimental demonstrating noise-driven pressure and temperature ***-driven sensors offer a new opportunity for delivering practical NEMS sensors that can function at room temperature and under ambient pressure,and a development that suggests a path to cheaper,simpler,and low-power-consumption sensors.

关键词： noise resonant overcome

Emerging mechanisms and implications of c GAS-STING signaling in cancer immunotherapy strategies

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Cancer Biology & Medicine 2024年第1期21卷 45-64页

作者： Jiawen Zhang Sihui Yu qiao Peng Ping Wang Lan Fang tongji University Cancer Center Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200072China Reproductive Medicine Center Department of Obstetrics and GynecologyShanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai 201620China Department of Obstetrics and Gynecology Zhongshan HospitalFudan UniversityShanghai 200032China

The intricate interplay between the human immune system and cancer development underscores the central role of immunotherapy in cancer *** this landscape,the innate immune system,a critical sentinel protecting against tumor incursion,is a key *** cyclic GMP-AMP synthase(c GAS)and stimulator of interferon genes(STING)pathway has been found to be a linchpin of innate immunity:activation of this signaling pathway orchestrates the production of type I interferon(IFN-α/β),thus fostering the maturation,differentiation,and mobilization of immune effectors in the tumor ***,STING activation facilitates the release and presentation of tumor antigens,and therefore is an attractive target for cancer *** strategies to activate the STING pathway,including use of pharmacological agonists,have made substantial advancements,particularly when combined with immune checkpoint *** approaches have shown promise in preclinical and clinical settings,by enhancing patient survival *** review describes the evolving understanding of the c GAS-STING pathway's involvement in tumor biology and ***,this review explores classical and non-classical STING agonists,providing insights into their mechanisms of action and potential for optimizing immunotherapy *** challenges and complexities,the c GAS-STING pathway,a promising avenue for enhancing cancer treatment efficacy,has the potential to revolutionize patient outcomes.

关键词： cGAS-STING pathway type I interferon cyclic dinucleotide STING agonist cancer immunotherapy

Tailoring the austenite characteristics via dual nanoparticles to synergistically optimize the strength-ductility in cold rolled medium Mn steel

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Journal of Materials Science & Technology 2024年第2期169卷 158-171页

作者： Qizhe Ye Haokai Dong Qinyi Guo Yishuang Yu Lijie qiao Yu Yan Beijing Advanced Innovation Center for Materials Genome Engineering Corrosion and Protection CenterInstitute for Advanced Materials and TechnologyUniversity of Science and Technology BeijingBeijing 100083China School of Mechanical and Automotive Engineering South China University of TechnologyGuangzhou 510641China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China School of Biomedical Engineering Shanghai Jiao Tong UniversityShanghai 200240China

In this work,we proposed a novel Cu/θdual nanoparticles strategy to tailor the austenite characteris-tics of a medium Mn steel via a tempering-annealing process to optimize the mechanical *** explored the effects of Cu-rich particles and cementite precipitated in the tempering process on the austenite reversion during the subsequent annealing *** experiments and numerical simula-tions verified that the austenite inherited from cementite had a finer size and a higher Mn enrichment compared with the austenite originating from the tempered martensite *** addition,quantitative evaluations revealed that the pinning effect exerted by the Cu-rich particles could significantly hinder theα/γinterface migration and the recrystallized grain growth,thereby further refining the final *** contributions from the effects of dual nanoprecipitates on the austenite reversion,the heterogeneous austenite grains inherited from varying nucleation sites ensured the sustained and gradual deformation-induced martensite and twinning ***,the Cu-added steels subjected to a tempering-annealing process achieved synergetic enhancement of the tensile strength from 1055 MPa to 1250 MPa and elongation from 33%to 45%.This strategy may provide new guidance for the development and alloy design of high-performance medium Mn steels.

关键词： Dual nanoparticles Medium Mn steel Tempering-annealing process Austenite reversion Mechanical properties

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船舶主机气囊隔振系统动态特性分析

振动与冲击 2007年第7期26卷 122-124页

作者：徐伟何琳吕志强李铜桥海军工程大学振动与噪声研究所武汉430033 海军驻哈尔滨汽轮机厂代表室哈尔滨150046

提出了将气囊隔振器应用于船舶主机隔振,介绍了该隔振装置的特性,并进行了动力学分析。分析表明,气囊隔振系统能够大幅度减小主机激振力向船体基座的传递。在其他条件不变时,系统具有定常频率特性,各气囊压力的分布对系统固有频率、力... 详细信息

提出了将气囊隔振器应用于船舶主机隔振,介绍了该隔振装置的特性,并进行了动力学分析。分析表明,气囊隔振系统能够大幅度减小主机激振力向船体基座的传递。在其他条件不变时,系统具有定常频率特性,各气囊压力的分布对系统固有频率、力传递特性没有影响,而对主机输出端的位移有一定影响,应该通过优化设计使联轴节的振动最小。

关键词：船舶主机气囊隔振系统动态特性

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考虑收益的航空升舱服务产品动态定价方法

交通运输系统工程与信息 2023年第3期23卷 76-84页

作者：赵桂红马晨傲赵巧同李建伏中国民航大学经济与管理学院天津300300 中国民航大学计算机科学与技术学院天津300300 维克森林大学商学院美国温斯顿-塞勒姆NC 27109

为使升舱服务产品实现旅客和航空公司利益共赢,本文构建旅客与航空公司均衡效用模型,采用Weibull分布函数刻画旅客对特定价格接受概率,并将其嵌入均衡效用模型,得出旅客在不同选择概率下的升舱服务产品价格;同时,为使模型更符合实际,在... 详细信息

为使升舱服务产品实现旅客和航空公司利益共赢,本文构建旅客与航空公司均衡效用模型,采用Weibull分布函数刻画旅客对特定价格接受概率,并将其嵌入均衡效用模型,得出旅客在不同选择概率下的升舱服务产品价格;同时,为使模型更符合实际,在模型中加入升舱服务产品的价格约束以及座位数约束,并证明在收益最大情况下,升舱服务最优价格一定在其价格约束区间内;最后,通过算例分别分析单一等级舱位升舱和多等级舱位升舱问题,得出两种情况下对不同舱位提供升舱服务产品的最优价格,以及对应动态定价策略下航空公司的收益。研究结果表明:与传统静态定价相比,若航空公司在升舱服务产品定价时考虑旅客需求效用,既能减少航空公司高端舱位的空置率,提高收益,又能使旅客以较低的价格享受升舱服务。此外,相比于单一等级升舱服务产品,多等级升舱服务产品可给航空公司带来更多收益,算例分析表明,航空公司销售两等级升舱服务产品比仅销售单一等级升舱服务产品带来的收益增加39.12%。

关键词：航空运输动态定价均衡效用升舱服务航班收益 Weibull分布

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基于CMOS的低功耗基准电路的设计

微电子学与计算机 2006年第4期23卷 80-82页

作者：童志强邹雪城童乔凌华中科技大学电子科学与技术系湖北武汉430074

基于带隙基准的原理,采用0.6μm、N阱CMOS工艺,文章设计了一种工作在亚阈值区的用于锂离子和锂聚合物电池充电保护芯片的低功耗基准电路。Hspice仿真结果表明:基准电压为1.068V,电源电压由1.8V到8V变化,电路最大消耗电流小于0.15μA;温... 详细信息

基于带隙基准的原理,采用0.6μm、N阱CMOS工艺,文章设计了一种工作在亚阈值区的用于锂离子和锂聚合物电池充电保护芯片的低功耗基准电路。Hspice仿真结果表明:基准电压为1.068V,电源电压由1.8V到8V变化,电路最大消耗电流小于0.15μA;温度由-40℃到80℃变化,其温度系数约为±10ppm/℃。整个充电保护芯片测试结果,其功耗小于0.6μW。

关键词：亚阚值区 CMOS工艺低功耗带隙基准温度系数

蛋白酶体抑制剂硼替佐米及其在肝脏疾病中作用的研究进展

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中华肝脏病杂志 2012年第9期20卷 712-714页

作者：任章朋孙立平童巧霞华中科技大学同济医学院附属协和医院感染科武汉430022

泛素-蛋白酶体通路（ubiquitin-proteasome pathway，UPP）是生物体内进行蛋白质选择性降解的重要途径之一，它广泛参与多种病理生理过程，如细胞周期调控以及信号转导、细胞凋亡、基因转录、受体胞吞、抗原呈递、免疫及炎症反应的调节... 详细信息

泛素-蛋白酶体通路（ubiquitin-proteasome pathway，UPP）是生物体内进行蛋白质选择性降解的重要途径之一，它广泛参与多种病理生理过程，如细胞周期调控以及信号转导、细胞凋亡、基因转录、受体胞吞、抗原呈递、免疫及炎症反应的调节等。其中，蛋白酶体是一种广泛存在的酶复合体，它参与细胞周期调控、细胞凋亡、血管形成中多种蛋白质的降解等过程，从而在细胞存活、纤维化和肿瘤发生和发展过程中起重要作用。因此，应用蛋白酶体抑制剂阻断UPP这个通路已成为近年来抗肿瘤治疗的研究热点。

关键词：肽水解酶类蛋白酶体抑制药肝疾病硼替佐米