检索结果-南通市图书馆

世界核心医学期刊文摘（胃肠病学分册） 2005年第1期1卷 53-53页

作者： Ishii m. Sugai T. HabanoW. Nakamura S.-I. 姜建涛 Division of Pathology Central Clinic Laboratory Iwate Medical University Moriaka 020-8505 Japan

Background. The point mutations occurring in codons 12 and 13 of the ki-ras gene are useful genetic markers to identify intratumoral heterogeneity. A single tumor crypt, which consists of monoclonal cells, can be obtained using the crypt isolation method. ki-ras gene mutations have been examined using the crypt isolation method to determine whether multiclonarity is present within the same tumor. methods. ki-ras gene mutations were analyzed using a crypt isolation technique coupled with polymerase chain reaction and direct sequencing in 21 sporadic colorectal carcinomas. The specimens were divided into two groups: a representative sample, which was composed of more than 50 tumor crypts, and a single tumor crypt sample. The latter consisted of 10 single tumor crypts, which were obtained from the same tumor separately. Results. ki-ras gene mutations were found in 11 of 21 representative samples and in 12 of 21 single tumor crypt samples. In the 11 samples with ki-ras mutation, ki-ras mutations were also found in most single tumor crypts. Among the 12 base substitutions found, G:C to A:T transitions were the most commonly observed. There were no differences between the two samples in the types of ki-ras mutations found. One ki-ras mutation that was not detected in the representative sample was observed in only a single tumor crypt. Conclusions. most carcinomas appear to have a homogeneous composition that may result from the successful progression of one of the clones having a ki-ras mutation. Additional mutations in the ki-ras gene were rarely observed in colorectal carcinomas.

关键词：腺管结直肠癌 ki-ras基因基因突变聚合酶链反应遗传标志分离技术碱基替换异质性序列分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases

引用

World Journal of Gastroenterology 2001年第3期7卷 381-386页

作者： Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang medical University in 1984,earned master degree in 1990,studied in the Surgical Department of kiel University and worked in the Lab of molecular Oncology of kiel University from 1994-1997achieved m.D.from kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. Department of General Surgery First Affiliated Hospital College of Medicine Zhejiang University 79 Qingchun Road Hangzhou 310003 Zhejiang ProvinceChina. Cjhui@***

AIm: To establish a relevant animal model of human gastrointestinal cancer, which can be used for repetitive investigations, so as to improve our understanding and management of carcinogenesis and cancer metastasis. mETHODS: Intact tissues of human colorectal and pancreatic cancers were transplanted in nude mice. The biological characteristics of the original and the corresponding transplanted tumors were investigated by HE staining, PAS staining and immunostaining. The metastases in the livers and lungs of nude mice were investigated by immunostaining with biotinylated mab KL-1 and by RT-PCR using CK20 specific primers. RESULTS: There were totally 9 of 16 surgical specimens growing in nude mice subcutaneously and/or orthotopically (4 of 6 colorectal and 5 of 10 pancreatic cancer). Tumor cell content of the specimens and freezing of tissue specimens are important factors influencing the growth of transplanted tumor. In the group of fresh tumor tissues with greater than 50% tumor cell content, the success rate of the transplantation was 100% (3 cases of pancreatic cancer and 3 cases of colorectal cancer). The orthotopically trans-planted tumors resemble the original tumor morphologically and biologically, including TAA expression such as CEA by immunohistochemistry, and CEA level in the serum of mice. ki-67 labeling index and the expression of TAA especially K-ras, 17-1A and RA-96, are associated with the potential of tumor growth in nude mice. micrometastases in the lungs and livers of tumor bearing mice can be detected by immunostaining with biotinylated mab KL-1 and CK20-specific RT-PCR. CONCLUSION: An orthotopic transplantation model for human colon and pancreatic cancer in nude mice has been set up. We have also established sensitive detection methods with CK-immunohistochemistry and CK20-RT-PCR to study xenotransplanted human cancer and its metastatic cancer cells in the liver and lung of nude mice. This study may be helpful in understanding the mechanism of ca

关键词： Animals Disease models, Animal Female Gastrointestinal Neoplasms Humans male mice mice, Nude Neoplasm Seeding Neoplasm Transplantation Research Support, Non-U.S. Gov't Transplantation, Heterologous

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

幽门螺杆菌感染影响人胃窦部主要分泌细胞群

引用

世界核心医学期刊文摘（胃肠病学分册） 2005年第10期1卷 12-12页

作者： VanDe Bovenkamp J.H.B. Korteland-Vanmale A.m. Büller H.A. J. Dekker 雷小英 Rudolf magnus Institute of Neuroscience Universiteitsweg 100 3584 CG Utrecht Netherlands Dr.

We have investigated how gastric H. pylori infection affects antrum secretory cell types by studying the expression of secretory proteins in antrum epithelium. Antrum biopsy specimens were prospectively collected from 102 individuals (49 H. pylori-infected). Immunohistochemistry was performed for secretory mucins (mUC5AC, mUC5B, mUC6), Trefoil factor family (TFF)-peptides (TFF1, TF-F2), endocrine peptides (gastrin, chromogranin A), and proliferating cells (ki-67). Protein expression was quant-ified morphometrically. H. pylori infection was significantly correlated to mucosal inflammation and to epithelial atrophy and proliferation. In H. pylori-infected patients the number of proliferating cells increased significantly, and the zone of proliferating cells shifted toward the surface epithelium of the antral glands. Infection was correlated with decreased mUC5AC, TFF1, and TFF2 expression and increased mUC6 and mUC5B expression. Endocrine cells expressing chromagranin A and gastrin shifted toward the surface epithelium of the antral glands in H. pylori-infected patients. H. pylori infection and concomitant inflammation induced increased epithelial proliferation and triggered coordinate deregulation of secretory cell populations in the antrum. In particular, infection led to a coordinated increase in cells expressing mUC6 and mUC5B at the expense of mUC5AC-producing cells.

关键词：细胞群部主三叶因子分泌细胞嗜铬粒蛋白胃泌素黏膜炎症免疫组化法表层细胞活检标本

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

乳腺癌18F-FDG PET/CT显像的瘤内异质性纹理特征分析在疗效和预后预测中的作用

引用

中华核医学与分子影像杂志 2018年第11期38卷 774-774页

作者：唐军 David molina-García Ana maría García-Vicente Julián Pérez-Beteta mariano Amo-Salas Alicia martínez-González maría Jesús Tello-Galán Angel Soriano-Castrejón Víctor m.Pérez-García 上海交通大学医学院附属苏州九龙医院核医学科 mathematical Oncology Laboratory (moLAB) Universidad de Castilla-La Mancha

目的评价局部晚期乳腺癌(LABC)F-脱氧葡萄糖(FDG)PET/CT显像的纹理分析参数在疗效和预后预测中的价值。方法该前瞻性研究包括68例LABC患者,纳入者均有新辅助化疗(NC)指征,并在化疗前行F-FDG PET/CT显像。对乳腺癌穿刺标本依据分子表型... 详细信息

目的评价局部晚期乳腺癌(LABC)F-脱氧葡萄糖(FDG)PET/CT显像的纹理分析参数在疗效和预后预测中的价值。方法该前瞻性研究包括68例LABC患者,纳入者均有新辅助化疗(NC)指征,并在化疗前行F-FDG PET/CT显像。对乳腺癌穿刺标本依据分子表型进行分组[雌激素受体(ER)、孕激素受体(PR)及人表皮生长因子2(HER2)的表达、细胞增殖核抗原ki-67指数等)],并在NC结束后将患者分为有效组和无效组。所有患者经标准的随访后得到无病生存时间(DFS)和总生存时间(OS)。利用计算机软件对乳腺癌病灶进行三维(3D)图像分割,随后进行纹理特征分析,获得行程长度矩阵(RLm)和共生矩阵(Cm)参数。对不同危险度的纹理特征与各种分子表型的关系进行分析。使用Kaplan-meier生存分析和Cox比例风险分析对纹理参数、分子表型和NC的肿瘤组织学反应等在预测疗效和预后中的作用进行研究。通过受试者工作特征(ROC)曲线分析取得最佳截断值、曲线下面积(AUC)及与OS、DFS相关的灵敏度和特异性。结果 NC对18例患者有效,其DFS和OS分别为(70.87±21.85)个月和(76.77±18.80)个月。长行程增强(LRE)和长行程高灰阶增强(LRHGE)与不同的危险度分层有关,而低灰阶行程增强(LGRE)、LRHGE和行程长度不均匀性(RLNU)均与NC的疗效相关。单因素分析结果表明纹理特征分析各变量与OS和DFS相关。多因素Cox回归分析结果显示,短行程高灰阶强化(SRHGE)下的PET分期与OS相关,而PET分期、高灰阶行程强化(HGRE)均与DFS相关。结论F-FDG PET/CT图像的纹理特征分析各变量可预测新辅助化疗的疗效和预后,并在预测OS和DFS时与PET分期具有相关性。

关键词：乳腺癌 18F-FDG PET/CT 纹理特征新辅助化疗疗效总生存时间无病生存时间

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：