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Detection of Human Papillomavirus DNA and E6/E7 mRNA from HPV Genotypes 16, 18, 31 and 33 in Histologically Confirmed Cases of Cervical Cancer and Precancerous Lesions in Burkina Faso

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American Journal of molecular Biology 2023年第4期13卷 276-290页

作者： Théodora mahoukèdè Zohoncon Shoukrat Ohuwa Toyin Bello Prosper Bado Rogomenoma Alice Ouédraogo Estelle Ouédraogo Ina marie Angèle Traoré Abdoul Karim Ouattara Florencia Wenkunni Djigma Albert Théophane Yonli Assita Sanou-Lamien Olga mélanie Lompo Jacques Simpore Laboratoire de Biologie moléculaire et de Génétique (LABIOGENE) Université Joseph KI-ZERBO Ouagadougou Burkina Faso Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Faculté des Sciences de la Santé Université Saint Thomas d’Aquin (USTA) Ouagadougou Burkina Faso Centre Hospitalo-Universitaire Yalgado Ouedraogo Ouagadougou Burkina Faso Pathological Anatomy and Cytology Laboratory Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Introduction: Cervical cancer, caused by persistent high-risk human papillomavirus (HPV) infection, remains a global public health problem. The cellular transformation and maintenance of the malignant phenotype of these HPVs are attributed to the viral oncoproteins E6 and E7. Objective: This study aims to detect the presence of human papillomavirus DNA and E6/E7 oncoprotein mRNA of HPV genotypes 16, 18, 31 and 33 in cases of cervical cancer and precancerous lesions, histologically confirmed in Burkina Faso. methods: This descriptive cross-sectional study focused on cases of cervical cancer and high-grade intraepithelial neoplasia (CIN) and was conducted from June to December 2022. One hundred (100) samples of fixed and paraffin-embedded tissues were collected from the pathological anatomy and cytology laboratories of hospitals in the capital of Burkina Faso. High-risk human papillomavirus (HR-HPV) DNA was detected using multiplex real-time PCR, while the presence of E6 and E7 mRNA in cervical cancer and high-grade CIN samples was determined using real-time Reverse Transcriptase-PCR (RT-PCR) with Taqman probes. Results: The mean age of women diagnosed with cervical cancer and high-grade CIN was 50.81 ± 13.65 years, ranging from 22 to 82 years. Cervical cancer and high-grade CIN were positive for at least one high-risk human papillomavirus (HR-HPV) in 80% of cases. The most prevalent genotypes observed were HPV16, 18, 31, and 33, collectively accounting for 70.08% of cases. Of the 89 samples that tested positive for HR-HPV genotypes 16, 18, 31, and 33, 88 (98.88%;95% CI: [94.58 - 99.94]) were also positive for the presence of mRNA encoding the E6 and E7 oncoproteins of HPV16, 18, 31, and 33. Conclusion: In the presence of HPV DNA, testing for E6 and E7 oncoprotein mRNA could serve as a promising biomarker and valuable tool for improved assessment of the progression to cervical cancer.

关键词： HPV E6/E7 Cervical Cancer Precancerous Lesions Burkina Faso

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几种三阴性乳腺癌标志物的临床病理意义

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中华病理学杂志 2017年第1期46卷 2-8页

作者：黄小彦池瑞霞刘铭李景曦曾婉姗谢文杰澳门仁伯爵综合医院病理解剖科新彊医科大学第一附属医院病理科香港中文大学威尔斯医院病理解剖及细胞学系

乳腺癌是一种高度异质性肿瘤，这种异质性体现在不同肿瘤类型之间以及同一肿瘤内部之间等多层次的差异，亦表现在传统的组织病理分型／免疫组织化学（ immunohistochemical ， IHC ）特征的分型和近期依据基因表达谱（ gene expression ... 详细信息

乳腺癌是一种高度异质性肿瘤，这种异质性体现在不同肿瘤类型之间以及同一肿瘤内部之间等多层次的差异，亦表现在传统的组织病理分型／免疫组织化学（ immunohistochemical ， IHC ）特征的分型和近期依据基因表达谱（ gene expression profiling ， GEP）的分子分型上［1］。根据最新版WHO分类，基于不同的生长方式、组织学结构和细胞学形态，乳腺癌可分为21种不同的组织学类型［2］。不同类型的肿瘤具有不同的组织学形态、基因表达及预后特征。使用分子分型可将乳腺癌分为不同的亚型，包括表达雌激素受体（ ER）相关因子的亚型（ luminal型）、表达人类表皮生长因子受体2（ HER2）相关通路因子的亚型（ HER2过表达亚型）和表达基底因子但不表达激素受体通路的亚型（ basal like breast cancer ， BLBC ）。在日常应用中，依据GEP分析的分子分型常常被IHC分型所替代。通过IHC分型方法［3］，乳腺癌被划分为激素受体（ ER、PR）阳性组和阴性组，后者根据HER2表达情况进一步分为HER2过表达乳腺癌和三阴性（ ER、PR、HER2阴性）乳腺癌（ triple negative breast carcinoma ， TNBC ）。2013年， St Gallen国际乳腺癌会议专家组再次确认IHC分类法，以乳腺癌IHC分型替代GEP分子分型：luminal A型（ ER ＋， PR≥20％， HER2－， ki-67＜20％）；luminal B型（ ER＋， PR＜20％和／或HER2＋和／或ki-67≥20％）；HER2过表达型（ ER －， PR －， HER2＋）；基底样型（ER－，PR－，HER2－）［4］。尽管这种IHC分类方法便于使用，但与GEP分型并不完全一致［4-5］。

关键词：乳腺癌 HER2过表达阴性临床病理癌标志物人类表皮生长因子受体2 组织病理分型 carcinoma

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糖尿病易感基因Clecl6a调控线粒体自噬

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中华糖尿病杂志 2014年第12期 923-923页

作者：高莉武晓泓于淼 Soleimanpour SA Gupta A Bakay m

研究显示,C.型凝集素域家庭16成员A(Clecl 6a;ki AA0350)是1型糖尿病、多发性硬化症和肾上腺功能障碍疾病的一种易感基因,但其功能尚不清楚。来自美国宾夕法尼亚大学佩雷尔曼医学院的Sol ei manpour等通过使用同位素随机或有针对性的差...

研究显示,C.型凝集素域家庭16成员A(Clecl 6a;ki AA0350)是1型糖尿病、多发性硬化症和肾上腺功能障碍疾病的一种易感基因,但其功能尚不清楚。来自美国宾夕法尼亚大学佩雷尔曼医学院的Sol ei manpour等通过使用同位素随机或有针对性的差异化的交互技术(I-DIRT)、质谱分析及免疫共沉淀方法 ,发现Clecl 6a是一种与E3泛素连

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kiT是胰腺内分泌肿瘤的独立预后标记

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临床与实验病理学杂志 2009年第6期25卷 634-634页

作者： Zhang L Smyrk T C Oliveira A m( et al.) 郎志强(摘译) 不详烟台毓璜顶医院病理科烟台264000

单凭组织学特点预测胰腺内分泌肿瘤（PET）的预后比较困难，最可靠的依据是转移或临近器官受累。但对于有些PET，尤其是无功能性肿瘤是影像学检查偶然发现的，并没有转移或局部浸润的证据，就很难预测其临床行为。WHO分类使用肿瘤大小... 详细信息

单凭组织学特点预测胰腺内分泌肿瘤（PET）的预后比较困难，最可靠的依据是转移或临近器官受累。但对于有些PET，尤其是无功能性肿瘤是影像学检查偶然发现的，并没有转移或局部浸润的证据，就很难预测其临床行为。WHO分类使用肿瘤大小、血管浸润、核分裂计数和ki-67指数作为预后标准。

关键词：胰腺内分泌肿瘤预后 kiT ki-67指数无功能性肿瘤影像学检查 WHO分类核分裂计数

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铬钼和铬钼钒压力容器钢的氢脆问题

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大型铸锻件 1983年第2期 F0003-F0003页

作者： m.Tvrdy

本文对四种Crmo和CrmoV压力容器钢在各种不同的热处理条件下测量与时间有关的ki值与断裂的变化关系和在氢环境下kiSH的临界值。假定CrmoV各种等级的钢比具有相应强度的各类Crmo钢更不易氢脆,前者所表现的kiSH临界值比后者高。这些发现... 详细信息

本文对四种Crmo和CrmoV压力容器钢在各种不同的热处理条件下测量与时间有关的ki值与断裂的变化关系和在氢环境下kiSH的临界值。假定CrmoV各种等级的钢比具有相应强度的各类Crmo钢更不易氢脆,前者所表现的kiSH临界值比后者高。这些发现是针对当前出现的各种合金元素及其对于在奥氮体晶界区有害杂质元素的显微偏析的影响进行分析的,晶界区的这些杂质元素可能与氢元素组合,从而造成晶间断裂。未文还对这些材料的稳定性裂纹增长作了力学方面的研究。

关键词：压力容器钢氢脆杂质元素晶间断裂热处理条件 Crmo钢显微偏析合金元素

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Detection of Low-Risk and High-Risk Oncogenic Human Papillomavirus in Archived Tissues from ENT Tumors in Burkina Faso

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Journal of Biosciences and medicines 2023年第9期11卷 171-180页

作者： Ali Kandé maïmouna Ilboudo Djénéba Ouermi Ina marie Angèle Traore Abdoul Karim Ouattara Théodora mahoukèdè Zohoncon Esther mah Alima Traore Florencia W. Djigma Dorcas Obiri-Yeboah Henriette Poaty Yvette marie Chantal Gyebre Olga m. Lompo Charlemagne mR Ouedraogo Jacques Simpore Centre de Recherche Biomoléculaire Pietro Annigoni (CERBA) Ouagadougou Burkina Faso Laboratoire de Biologie moléculaire et de génétique (LABIOGENE) UFR/SVT Université Joseph Ki-Zerbo Ouagadougou Burkina Faso University Post Office Department of Microbiology and Immunology School of Medical Sciences University of Cape Coast Cape Coast Ghana Centre Hospitalier Universitaire Yalgado Ouedraogo Université Joseph Ki-Zerbo Ouagadougou Burkina Faso

Human papillomavirus (HPV) is classified into high-risk HPV (HR-HPV) and HPV (LR-HPV) according to their oncogenic potential. These viruses can be found in the cervix, vagina, vulva, anus and in the ENT sphere. HPV ENT infections can lead to benign or malignant tumors in which we could find both LR-HPV and HR-HPV genotypes. The objective of this study was to investigate the genotypes of HR-HPV and LR-HPV in archived tissue samples derived from both benign and malignant tumors of the ear, nose, and throat (ENT) in Ouagadougou, Burkina Faso. One hundred and twenty formalin-fixed, paraffin-embedded archived tissues of the ENT sphere from 26 benign tumors and 94 malignant tumors were included. The tissues were first deparaffinized with xylem. The extracted DNA was used to test for high-risk and low-risk HPV by Real-Time multiplex PCR. HPV DNA was found in 57.7% (15/26) of benign tumors and 43.61% (41/94) of malignant tumors. The prevalence of HPV infection was 46.67% (56/120) in all tumors combined. The most common HPV genotypes found were HPV 11 (34.28%), HPV 6 (30%), HPV56 (14.28%) and HPV 33 (8.57%). There were 21.43% (12/56) cases of genotypes co-infections with 10 cases of double infection and 2 cases of triple infection. Both low-risk and high-risk HPV are found in ENT tumors with relatively high HPV prevalence.

关键词： HPV Archived Tissues ENT Tumor Burkina Faso

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Effects of Hydrolysed Collagen Associated or Not with Physical Agents on Tissue and muscle Flaccidity—Firmez method: Case Study

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Open Journal of Applied Sciences 2022年第1期12卷 82-98页

作者： Rafaella Rêgo maia Patrícia Froes meyer Rodrigo marcel Valentim Da Silva Eneida De morais Carreiro Thaynar Gonzaga Da Silva Lígia De Oliveira Revorêdo Stephany Luanna Queiroga Farias Ciro Dantas Soares michelle Cordeiro Sanz mariana Adélia Girotto Bussade José Dos Santos Bussade mônica Etrusco Pedrosa Federal University of Rio Grande do Norte Natal Brazil University Centre of Rio Grande do Norte (UNIRN) Natal Brazil Estácio de Sá University Natal Brazil Potiguar University—UNP Natal Brazil Campinas State University (UNICAmP) Campinas Brazil Higher School of Sciences of Santa Casa de misericórdia Vitória Brazil University of Säo Judas Tadeu S&#228o Paulo Brazil Federal University of Rio de Janeiro Rio de Janeiro Brazil University of medical Sciences of minas Gerais Minas Gerais Brazil

The combination of therapies may be the promising future for the treatment of esthetic alterations more efficiently and in less time, in order to obtain an excellent result. Our objective was to evaluate the effectiveness of hydrolysed collagen associated with radiofrequency and electrostimulation in the treatment of flaccidity in the abdominal and gluteal regions. The sample consisted of 6 women, evaluated through evaluation protocols, questionnaires, dynamometry, ultrasound exams, and histological analysis. The volunteers were distributed into three subgroups: hydrolysed collagen group (G-1), radiofrequency + hydrolysed collagen group (G-2) and radiofrequency + hydrolysed collagen + electrostimulation group (G-3). The ultrasound analysis of the abdominal muscles showed an increase in muscle thickness in all groups, but not significant (p > 0.05). The histological analysis showed an evident increase in type I collagen in groups G-2 and G-3, with proliferating cells (Ki67+). The results of dynamometry showed significant values in different times (p mbilical perimetry analysis, when compared between the groups with 30 days (p = 0.03) and with 60 days (p = 0.04). All participants rated the treatment positively. The use of combined therapies has shown superior results for the treatment of abdominal and gluteal flaccidity when compared with its isolated use, being the combination of therapies important in the treatment of flaccidity.

关键词： Sagging Collagen Radiofrequency Electro-Stimulation

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Her-2和ki-67在胃癌组织中的表达及其相关性研究

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长治医学院学报 2017年第2期31卷 93-97页

作者：叶海主何连君袁倩倩赵文英刘晓平皖南医学院第一附属医院弋矶山医院 241001

目的:检测胃癌组织标本中Her-2及ki-67的表达情况并探究其相关性及两者与肿瘤临床病理参数关系。方法:收集胃腺癌组织标本213例,免疫组织化学(IHC)检测Her-2和ki-67的表达状态;荧光原位杂交(FISH)进一步检测Her-2基因扩增情况并分析它... 详细信息

目的:检测胃癌组织标本中Her-2及ki-67的表达情况并探究其相关性及两者与肿瘤临床病理参数关系。方法:收集胃腺癌组织标本213例,免疫组织化学(IHC)检测Her-2和ki-67的表达状态;荧光原位杂交(FISH)进一步检测Her-2基因扩增情况并分析它们与临床病理参数之间关系。结果:213例胃腺癌组织中,Her-2和ki-67阳性表达率分别为39.44%(84/213)和81.22%(173/213),Her-2基因表达与肿瘤的浸润深度、分化程度、TNm分期和淋巴结转移相关(P0.05);ki-67表达与分化程度、TNm分期、浸润深度、淋巴结转移和有无脉管癌栓有关(P0.05)。Her-2基因扩增与ki-67高表达呈正相关(r=0.177,Pki-67表达情况对病情评估以及预后判断有重要意义,给个体化分子靶向治疗提供科学依据。

关键词：胃腺癌 Her-2 ki-67 免疫组化荧光原位杂交

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极早产儿童6岁时神经系统和发育性残疾

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世界核心医学期刊文摘(妇产科学分册) 2005年第5期 6-6页

作者： marlow N,Wolke D ,Bracewell m.A ,Samara m ,宁亮 Academic Division of Child Health Queens medical Centre East Block Nottingham NG7 2UH United kingdom Dr

BACKGROUND: Birth before 26 weeks of gestation is associated with a high preva lence of neurologic and developmental disabilities in the infant during the firs t two years of life. mETHODS: We studied at the time of early school age childre n who had been born at 25 or fewer completed weeks of gestation in the United ki ngdom and Ireland in 1995. Each child had been evaluated at 30 months of age. Th e children underwent standardized cognitive and neurologic assessments at six ye ars of age. Disability was defined as severe (indicating dependence on caregiver s), moderate, or mild according to predetermined criteria. RESULTS: Of 308 survi ving children, 241 (78 percent) were assessed at a median age of six years and f our months; 160 classmates delivered at full term served as a comparison group. Although the use of test reference norms showed that cognitive impairment (defin ed as results more than 2 SD below the mean) was present in 21 percent of the ch ildren born extremely preterm (as compared with 1 percent in the standardized da ta), this value rose to 41 percent when the results were compared with those for their classmates. The rates of severe, moderate, and mild disability were 22 pe rcent, 24 percent, and 34 percent, respectively; disabling cerebral palsy was pr esent in 30 children (12 percent). Among children with severe disability at 30 m onths of age, 86 percent still had moderate-to-severe disability at 6 years of age. In contrast, other disabilities at the age of 30 months were poorly predic tive of developmental problems at 6 years of age. CONCLUSIONS: Among extremely p reterm children, cognitive and neurologic impairment is common at school age. A comparison with their classroom peers indicates a level of impairment that is gr eater than is recognized with the use of standardized norms.

关键词：神经系统发育性儿童

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Research on rat models of hypobaric hypoxia-induced pulmonary hypertension

Research on rat models of hypobaric hypoxia-induced pulmonar...

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青藏铁路运营十周年学术研讨会

作者： T.-T.mA Y WANG X.-L.ZHOU H.JIANG R.GUO L.-N.JIA H.CHANG Y GAO X.-Y.YAO Z.-m.GAO L.PAN Department of Geriatric medicine Beijing Shijitan HospitalCapital Medical University Department of Hypoxia Laboratory Beijing Shijitan HospitalCapital Medical University Department of Pathology Beijing Shijitan HospitalCapital Medical University Health Science Center of Peking University

OBJECTIVE:Rat models of hypobaric hypoxia-induced pulmonary hypertension are commonly used in studies of chronic mountain sickness,while there are few researches specially focusing on these rats *** study aims to exploring possible pathogenesis of hypobaric hypoxia-induced pulmonary hypertension by experimenting on hypobaric hypoxiainduced PH rat models at different *** AND mETHODS:32 healthy male SD rats were randomly divided into six groups of different degree and time period of hypobaric *** mean pulmonary arterial pressure（m PAP）,right ventricular pressure（RVSP）,the right ventricle（RV）,left ventricular（LV）,ventricular septal（S）,the right ventricular hypertrophy index（RVHI）[calculated under the formula of RV/（LV + S）],hematoxylin-eosin staining,elastic fibers staining,the ratio of the thickness of vascular wall to its outer diameter（mT%）,the ratio of the cross-sectional area of the middle vascular wall to the total vascular cross-sectional area（mA%）;the a-SmA,and the ki6 expressions were detected to evaluated the pulmonary ***:There were significant differences of the mPAP,RVSP and RVHI value between the hypobaric hypoxia groups and the control group（p ＜ 0.05）.The mPAP,RVSP,RVHI,mT%,mA%,α-SmA,and ki6 of rats in model groups at an altitude of 3Km were higher than those of the control group,which raised gradually with the number of weeks *** mPAP,RVSP,RV/（LV + S） value,mT%,mA%,a-SmA,and ki67 of the 5Km-4W group were significantly higher than those of the control group（p ＜ 0.05）.CONCLUSIONS:Rat models with pulmonary hypertension at different altitudes have been successfully established by automatic adjusting hypobaric hypoxia *** to a low oxygen environment at a simulate-altitude of 3km for 8 weeks have caused the pathological remodeling of pulmonary vascular walls and pulmonary hypertension,and further led to a series of pathological changes,including right ventricular ***

关键词： Hypobaric hypoxia Pulmonary hypertension Animal models

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