检索结果-南通市图书馆

DEVELOPMENT OF NEEDLE-BASED MIcROENDOScOPY FOR FLUOREScENcE MOLEcULAR IMAGING OF BREAST TUMOR MODELS

Journal of Innovative Optical Health Sciences 2009年第4期2卷 343-352页

作者： cHAO-WEI cHEN tiffany R.BLAcKWELL RENEE NAPHAS PAUL T.WINNARD JR VENU RAMAN KRISTINE GLUNDE YU cHEN Fischell Department of Bioengineering and Electrical and computer Engineering University of Maryland College ParkMD 20742USA In Vivo cellular and Molecular Imaging center(IcMIc)Department of Radiology Johns Hopkins University School of Medicine BaltimoreMD 21205USA§yuchen@umd.edu¶kglunde@mri.jhu.edu

Fluorescence molecular imaging enables the visualization of basic molecular processes such as gene expression,enzyme activity,and disease-specific molecular interactions in vivo using targeted contrast agents,and therefore,is being developed for early detection and in situ characterization of breast *** advances in developing near-infrared fluorescent imaging contrast agents have enabled the specific labeling of human breast cancer cells in mouse model *** synergy with contrast agent development,this paper describes a needle-based fluorescence molecular imaging device that has the strong potential to be translated into clinical breast biopsy *** microendoscopy probe is based on a gradient-index(GRIN)lens interfaced with a laser scanning *** of the imaging performance,including the field-of-view,transverse resolution,and focus tracking characteristics were *** MDA-MB-231 breast cancer xenografts stably expressing the tdTomato red fluorescent protein(RFP)were used to detect the tumor cells in this tumor model as a proof of principle *** further development,this technology,in conjunction with the development of clinically applicable,injectable fluorescent molecular imaging agents,promises to perform fluorescence molecular imaging of breast cancers in vivo for breast biopsy guidance.

关键词： Breast cancer fluorescence molecular imaging microendoscopy optical imaging tdTomato fluorescent protein.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Blastomyces dermatitidis: Stability studies on different yeast lysate antigens

引用

Open Journal of Immunology 2013年第3期3卷 98-102页

作者： tiffany R. Allison Joshua c. Wright Gene M. Scalarone Department of Biological Sciences Idaho State University Pocatello USA

In Trial 1, 19 lots of Blastomyces dermatitidis (T-58;Tennessee dog isolate) were assayed to determine the stability of the reagents following storage. The reactivity of the antigens, produced from 1989 to 2012 and stored at 4°c, was determined by comparing antibody detection (enzyme-linked immunosorbent assay;ELISA) in 12 serum specimens from immunized rabbits. All of the 19 reagents produced during this 23-year period exhibited a high degree of stability and were able to detect antibody in the sera. Mean absorbance values ranged from 0.798 (1989) to 0.827 (2012) and a mean value for all 19 antigens of 0.728. In a related evaluation, Trial 2, B. dermatitidis lysate antigens prepared from 8 isolates (dog, human, soil) at two different time periods were assayed as above to determine reactivity. The time of storage between the first and second reagents varied from 4 to 17 years. The results indicated that all 16 of the lysate antigens detected antibody in the 15 rabbit serum specimens with mean absorbance values ranging from 0.346 to 0.682, but variations in reactivity were observed depending on the lysate and the serum specimen assayed. This comparative study provided evidence that the antigenic reagents do exhibit some lot-to-lot variation in reactivity, but they did not lose any appreciable potency during prolonged storage.

关键词： Blastomyces dermatitidis Lysate Antigens Antibody Detection Stability ELISA

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Accessibility to Oral Antiviral Therapy for Patients with chronic Hepatitis c in the United States

引用

Journal of clinical and Translational Hepatology 2016年第2期4卷 76-82页

作者： Sammy Saab Melissa Jimenez tiffany Fong crystal Wu Sherona Bau Zoha Jamal Jonathan Grotts David Elashoff Department of Medicine University of CaliforniaLos AngelesCAUSA Department of Surgery University of CaliforniaLos AngelesCAUSA Department of Surgery University of CaliforniaLos AngelesCAUSA Department of Biostatistics University of CaliforniaLos AngelesCAUSA

Background:Hepatitis c (HcV) direct acting antiviral agents (DAAs) are safe,effective,and *** contraindications to interferon-based treatment are no long *** aims of this study were to understand the predictors of approval to drug ***:We studied all consecutive patients with HcV prescribed DAAs between October 2014 and July *** on demographic,socioeconomic status,comorbidities,baseline laboratory values,and assessment of liver disease severity,insurance,and specialty pharmacy type were *** analyses were performed to identify predictors of prescription ***:In total,410 patients were prescribed DAAs between October 2014 and July *** those,332 (81%) patients were insurance approved for *** the 332 patients accepted,251 were accepted after the first prescription attempt,and 38 were accepted after the second and third *** number of attempts for the other 43 approved patients was *** age (p =0.001),employment (p =0.001),lack of comorbidities (p =0.02),liver transplantation (p =0.018),and advanced liver disease (p =0.001)were more likely associated with obtaining *** income was not associated with insurance *** the multivariate analysis,Medicare insurance (odds ratio [OR]) 2.67,95% confidence interval [cI] 0.96-7.20),lack of nonliver comorbidities (OR 2.72,95% cI 1.35-5.43),and the presence of advanced liver disease (OR 1.82,95% cI 1.04-3.24) independently predicted drug ***:Despite the availability of DAAs for HcV,barriers from insurance carriers continue to impair widespread *** with advanced liver disease,Medicare,and without comorbidities are most likely to be insurance approved for DAAs.

关键词： Hepatitis c Healthcare access Antiviral therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fabrication of all-transparent polymer-based and encapsulated nanofluidic devices using nano-indentation lithography

引用

Microsystems & Nanoengineering 2017年第1期3卷 373-381页

作者： cong Wu tiffany G.Lin Zhikun Zhan Yi Li Steve c.H.Tung William c.Tang Wen J.Li Department of Mechanical and Biomedical Engineering City University of Hong KongHong Kong 999077China Department of Biomedical Engineering University of CaliforniaIrvineCA 92697-2715USA Department of Automation Yanshan UniversityQinghuangdao 066004China Department of Mechanical Engineering University of ArkansasFayettevilleAR 72701USA

In this paper,we describe a novel and simple process for the fabrication of all-transparent and encapsulated polymeric nanofluidic devices using nano-indentation ***,a nanomechanical probe is used to‘scratch’nanoscale channels on polymethylmethacrylate(PMMA)substrates with sufficiently high ***,polydimethylsiloxane(PDMS)is used twice to duplicate the nanochannels onto PDMS substrates from the‘nano-scratched’PMMA substrates.A number of experiments are conducted to explore the relationships between the nano-indentation parameters and the nanochannel dimensions and to control the aspect ratio of the fabricated *** addition,traditional photolithography combined with soft lithography is employed to fabricate microchannels on another PDMS‘cap’*** manually aligning the substrates,all uncovered channels on two separate PDMS substrates are bonded to achieve a sealed and transparent nanofluidic device,which makes the dimensional transition from microscale to nanoscale *** smallest dimensions of the achievable nanochannels that we have demonstrated thus far are of~20 nm depth and~800 nm width,with lengths extendable beyond 100μ*** flow experiments are performed to verify the reliability of the *** types of colloidal solution are used to visualize the fluid flow through the nanochannels,that is,ethanol is mixed with gold colloid or fluorescent dye(fluorescein isothiocyanate),and the flow rate and filling time of liquid in the nanochannels are estimated based on time-lapsed image *** simplicity of the fabrication process,bio-compatibility of the polymer substrates,and optical transparency of the nanochannels for flow visualization are key characteristics of this approach that will be very useful for nanofluidic and biomolecular research applications in the future.

关键词： indentation lithography nanochannel nanofluidic device nanofluidic flow

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

鼠疫耶尔森菌纤维蛋白溶解酶原激活因子蛋白及其特异性片段的原核表达

引用

中国地方病学杂志 2012年第5期31卷 482-485页

作者：杜春红王鹏 tiffany c.Ho 董兴齐宋志忠吴锡南张建中云南省地方病防治所云南省鼠疫防控技术重点实验室大理671000 昆明医科大学公共卫生学院中国疾病预防控制中心传染病预防控制所

目的重组表达鼠疫耶尔森菌纤维蛋白溶解酶原激活因子（Plasminogen activator，PIa）蛋白及其特异性片段并检测其免疫反应性。方法以鼠疫EV76株为模板，PcR扩增pla,pla-c基因，并与质粒原核表达载体（prokaryotic expression vector，pE... 详细信息

目的重组表达鼠疫耶尔森菌纤维蛋白溶解酶原激活因子（Plasminogen activator，PIa）蛋白及其特异性片段并检测其免疫反应性。方法以鼠疫EV76株为模板，PcR扩增pla,pla-c基因，并与质粒原核表达载体（prokaryotic expression vector，pET）32a（＋）连接，将pET32a（＋）-pla、pET32a（＋）-pla—c分别转化***21（DE3）诱导表达；产物经镍离子金属螯合亲和层析纯化，免疫印迹（Westernblot）法鉴定其免疫反应性。结果所表达的Pla蛋白相对分子质量（Mr）为52．8×10。，以包涵体形式存在；Pla—c蛋白Mr为24．0x10s，以可溶形式存在；这两个蛋白质均可与鼠疫免疫血清产生特异结合反应。结论所表达的鼠疫菌Pla及其特异性片段具有免疫反应性，为辅助诊断鼠疫提供了选择。

关键词：鼠疫耶尔森菌纤维蛋白溶解酶原激活因子蛋白原核表达免疫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Full-field interferometric imaging of propagating action potentials

引用

Light(Science & Applications) 2018年第1期7卷 51-61页

作者： Tong Ling Kevin c.Boyle Georges Goetz Peng Zhou Yi Quan Felix S.Alfonso tiffany W.Huang Daniel Palanker Hansen Experimental Physics Laboratory Stanford UniversityStanfordCA 94305USA Department of Ophthalmology Stanford UniversityStanfordCA 94305USA Department of Electrical Engineering Stanford UniversityStanfordCA 94305USA Department of Molecular and cellular Physiology Howard Hughes Medical InstituteStanford UniversityStanfordCA 94305USA Department of chemistry Stanford UniversityStanfordCA 94305USA

currently,cellular action potentials are detected using either electrical recordings or exogenous fluorescent probes that sense the calcium concentration or transmembrane *** imaging has a low temporal resolution,while voltage indicators are vulnerable to phototoxicity,photobleaching,and ***,we report full-field interferometric imaging of individual action potentials by detecting movement across the entire cell *** spike-triggered averaging of movies synchronized with electrical recordings,we demonstrate deformations up to 3 nm(0.9 mrad)during the action potential in spiking HEK-293 cells,with a rise time of *** time course of the optically recorded spikes matches the electrical *** the shot noise limit of the camera(~2 mrad/pix)precludes detection of the action potential in a single frame,for all-optical spike detection,images are acquired at 50 kHz,and 50 frames are binned into 1 ms steps to achieve a sensitivity of 0.3 mrad in a single *** a selfreinforcing sensitivity enhancement algorithm based on iteratively expanding the region of interest for spatial averaging,individual spikes can be detected by matching the previously extracted template of the action potential with the optical *** allows all-optical full-field imaging of the propagating action potentials without exogeneous labels or electrodes.

关键词： action averaging recording

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Anti-inflammatory mechanisms of the novel cytokine interleukin-38 in allergic asthma

引用

cellular & Molecular Immunology 2020年第6期17卷 631-646页

作者： Xiaoyu Sun Tianheng Hou Edwin cheung tiffany Nga-Teng Iu Victor Wai-Hou Tam Ida Miu-Ting chu Miranda Sin-Man Tsang Paul Kay-Sheung chan christopher Wai-Kei Lam chun-Kwok Wong Department of chemical Pathology The Chinese University of Hong KongHong KongChina cancer centre/centre of Precision Medicine Research and Training Faculty of Health SciencesUniversity of MacaoMacaoChina Institute of chinese Medicine and State Key Laboratory of Research on Bioactivities and clinical Applications of Medicinal Plants The Chinese University of Hong KongHong KongChina Department of Microbiology The Chinese University of Hong KongHong KongChina Faculty of Medicine and State Key Laboratory of Quality Research in chinese Medicines Macao University of Science and TechnologyMacaoChina Li Dak Sum Yip Yio chin R&D centre for chinese Medicine The Chinese University of Hong KongHong KongChina

We elucidated the anti-inflammatory mechanisms of IL-38 in allergic *** bronchial epithelial cells and eosinophils were cocultured upon stimulation with the viral RLR ligand poly(I:c)/LyoVec or infection-related cytokine TNF-αto induce expression of cytokines/chemokines/adhesion *** dust mite(HDM)-induced allergic asthma and humanized allergic asthma NOD/ScID murine models were established to assess anti-inflammatory mechanisms in ***-38 significantly inhibited induced proinflammatory IL-6,IL-1β,ccL5,and cXcL10 production,and antiviral interferon-βand intercellular adhesion molecule-1 expression in the coculture *** cytometry and RNA-sequencing analysis revealed that IL-38 could antagonize the activation of the intracellular STAT1,STAT3,p38 MAPK,ERK1/2,and NF-κB pathways,and upregulate the expression of the host defense-related gene POU2AF1 and anti-allergic response gene *** injection of IL-38 into HDM-induced allergic asthma mice could ameliorate airway hyperreactivity by decreasing the accumulation of eosinophils in the lungs and inhibiting the expression of the Th2-related cytokines IL-4,IL-5,and IL-13 in the bronchoalveolar lavage fluid(BALF)and lung *** examination indicated lung inflammation was alleviated by reductions in cell infiltration and goblet cell hyperplasia,together with reduced Th2,Th17,and innate lymphoid type 2 cell numbers but increased proportions of regulatory T cells in the lungs,spleen,and lymph ***-38 administration suppressed airway hyperreactivity and asthma-related IL-4 and IL-5 expression in humanized mice,together with significantly decreased ccR3^(+) eosinophil numbers in the BALF and lungs,and a reduced percentage of human cD4^(+)cRTH2^(+)Th2 cells in the lungs and mediastinal lymph ***,our results demonstrated the anti-inflammatory mechanisms of IL-38 and provided a basis for the development of a regulatory cytokine-based treatment for allergic

关键词： allergic asthma eosinophils IL-38 ILc2s Tregs

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

鼠疫菌caf1基因特异性片段的克隆表达及其抗原与抗体反应

引用

中国媒介生物学及控制杂志 2012年第6期23卷 489-491,495页

作者：杜春红王鹏 tiffany c Ho 张建中宋志忠董兴齐云南省地方病防治所云南省鼠疫防控技术重点实验室云南大理671000 中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室北京102206

目的克隆表达鼠疫菌caf1基因上的特异性片段并检测。方法分别选取F1上c端(caf1-c)及N端(caf1-N)两段B细胞表位进行克隆表达;PcR扩增目的基因,并与表达质粒pET32a(+)进行连接,重组质粒转化大肠埃希菌BL21(DE3)并诱导表达;表达产物经NiNT... 详细信息

目的克隆表达鼠疫菌caf1基因上的特异性片段并检测。方法分别选取F1上c端(caf1-c)及N端(caf1-N)两段B细胞表位进行克隆表达;PcR扩增目的基因,并与表达质粒pET32a(+)进行连接,重组质粒转化大肠埃希菌BL21(DE3)并诱导表达;表达产物经NiNTA亲和层析柱纯化,并以Westernblot法检测其抗原与抗体反应。结果分别诱导表达出大小为26000的caf1-c和25000的caf1-N两个片段,2个重组蛋白均以可溶形式存在,并与鼠疫免疫血清有良好的反应。结论克隆表达出具有抗原与抗体反应的鼠疫菌F1抗原上的两段B细胞表位,为鉴定是否是F1位点交叉奠定了基础。

关键词：鼠疫 F1抗原特异性片段克隆表达

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overuse and underuse of calcium and vitamin D in women with osteoporosis: A survey in a primary care setting

引用

Open Journal of Preventive Medicine 2013年第9期3卷 570-575页

作者： Milagros Silva Adam Romeiser David W. Baker Aashish K. Didwania tiffany Brown Joseph M. Feinglass Ji Young Lee Nancy c. Dolan Division of General Internal Medicine Department of Medicine Northwestern University Feinberg School of Medicine Chicago USA

Introduction: Optimal calcium and vitamin D intake is important components of the treatment of osteoporosis. The national average calcium and vitamin D intake for women over age 50 is below the recommended levels for optimal bone health. The aim of this study was to assess whether deficiencies in calcium and vitamin D intake exist in women with osteoporosis in a general medicine practice, as well as evaluate whether physicians accurately document calcium and vitamin D supplementation in the health record. Methods: Using the Electronic Health Record (EHR), we identified all female patients age 50 and older with the diagnosis of osteoporosis who were seen at the study site clinic, an urban academic general medicine practice, between January 1st, 2010 and December 1st, 2010. Women were randomly selected to receive an invitation to participate in a telephone survey on osteoporosis treatment. Results: One hundred and sixteen women completed the telephone survey. The mean calcium intake was 1524 mg per day. Forty-nine percent of women surveyed reported taking less than 1200 mg/day of supplemental calcium. When considering reported calcium intake from diet, 33% percent consumed less than 1200 mg/day and 52% percent consumed more than 1500 mg/day. Twenty eight percent of patients were taking less than 800 IU/day of vitamin D;only four percent were taking more than 4000 IU/day. Agreement between patients’ self-reported intake of calcium, vitamin D and multivitamin supplements and physicians’ documentation of these supplements was 52% for calcium, 53% for vitamin D and 61% for multivitamin. conclusion: Among women with osteoporosis getting regular care in a general medicine practice, approximately a third are getting less than the recommended daily amount of calcium and a quarter less than the recommended amount of vitamin D. In addition, a significant proportion of women are getting excessive daily amounts of calcium, which may also be a quality concern. Rates of agreement b

关键词： calcium Supplementation Ambulatory Electronic Health Record Vitamin Supplementation Osteoporosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

书桌般大小的连续波功率1千瓦cO2激光器

引用

真空电子技术 1971年第1期 12-14页

作者： William B. tiffany

普通的千瓦级输出功率的 cO2激光器,要求的典型激光长度为20米。目前,使用气体输运激光器能达到1千瓦的连续波输出,共有效长度仅为1米,其效率可与现有的最好的cO2激光器相此拟。此激光器结构紧凑,在汽车、飞机、核燃料、玻璃、陶瓷以及... 详细信息

普通的千瓦级输出功率的 cO2激光器,要求的典型激光长度为20米。目前,使用气体输运激光器能达到1千瓦的连续波输出,共有效长度仅为1米,其效率可与现有的最好的cO2激光器相此拟。此激光器结构紧凑,在汽车、飞机、核燃料、玻璃、陶瓷以及建筑等工业中可作为切割、打孔和焊接工具。

关键词：激光器输运 cO2 co 光激射器电子器件二氧化碳平均自由路程

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：